SSGCID: Target Page

BuviA.00010.y

Short-chain dehydrogenase/reductase SDR

CENTER ID:	BuviA.00010.y
ORGANISM:	Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuviA.00010.y.B1.GE39404	Full length( BuviA.00010.y )		1	267

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|269482.11.peg.2460
RefSeq:	YP_001116195.1
UniProt:	A4JKC7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQPLKQAANT PVVLVTGAAR RAGRAFAEYF ADHGYRTAIH YDRSADAAHT AARAIAERGH DSVALQADLS DAAQISALID QVYARFGRLD VLVNNASVFW QDHFPSFELA AFDQAWAVNC RAPILLTRAF YERARAAGAQ GVVVNVVDQK IKENFHRDHF SYTVAKAALG NLTQMLALSS APVLRVNAVF PGLMLPSDDQ TQADFEHASR ASTPLARIAG PDDVASAILL LTGSAYNGVD FVVDAGQNLI RVDQDVLYKH RSPAGKH

NT Sequence

ATGCAGCCCC TGAAGCAAGC GGCGAACACG CCCGTCGTGC TCGTGACCGG CGCCGCGCGC CGGGCCGGGC GCGCATTCGC CGAGTATTTC GCCGATCACG GCTACCGCAC CGCGATCCAC TACGATCGCT CGGCCGACGC CGCCCATACG GCTGCCCGCG CGATCGCCGA ACGCGGGCAC GATTCAGTCG CGCTGCAGGC CGACCTGTCG GATGCCGCGC AGATCAGCGC GCTGATCGAT CAGGTGTATG CGCGCTTCGG CCGCCTCGAC GTGCTGGTCA ACAATGCGTC GGTGTTCTGG CAGGACCATT TTCCGAGCTT CGAACTCGCG GCGTTCGATC AGGCGTGGGC CGTGAACTGC CGCGCGCCGA TCCTCCTCAC GCGTGCGTTC TACGAGCGGG CGCGCGCGGC CGGCGCGCAG GGCGTCGTGG TCAACGTGGT CGATCAGAAG ATCAAGGAGA ACTTCCACCG CGACCACTTC AGCTACACGG TCGCGAAGGC GGCGCTCGGC AACCTCACGC AGATGCTCGC GCTTTCGTCG GCGCCGGTGC TGCGCGTGAA CGCGGTGTTC CCGGGGCTGA TGCTGCCGAG CGACGATCAA ACGCAGGCCG ACTTCGAACA CGCGAGCCGT GCGTCGACAC CGCTCGCGCG CATCGCCGGC CCCGACGACG TGGCGAGCGC GATCCTGCTG CTCACCGGCA GCGCCTACAA CGGCGTGGAT TTCGTCGTCG ATGCGGGGCA GAACCTGATC CGCGTCGATC AGGACGTGCT GTACAAGCAC CGATCGCCGG CCGGCAAGCA T

Details for BuviA.00010.y.B1.GE39404

HARVESTED ON:	8/5/2015
SEQUENCED ON:	8/13/2015
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	98
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMQ PLKQAANTPV VLVTGAARRA GRAFAEYFAD HGYRTAIHYD RSADAAHTAA RAIAERGHDS VALQADLSDA AQISALIDQV YARFGRLDVL VNNASVFWQD HFPSFELAAF DQAWAVNCRA PILLTRAFYE RARAAGAQGV VVNVVDQKIK ENFHRDHFSY TVAKAALGNL TQMLALSSAP VLRVNAVFPG LMLPSDDQTQ ADFEHASRAS TPLARIAGPD DVASAILLLT GSAYNGVDFV VDAGQNLIRV DQDVLYKXXX PAGKH

Validated NT Sequence

atcttactca cttaatgctt gccggccggc nnnnnntgct tgtacagcac gtcctgatcg acgcggatca ggttctgccc cgcatcgacg acgaaatcca cgccgttgta ggcgctgccg gtgagcagca ggatcgcgct cgccacgtcg tcggggccgg cgatgcgcgc gagcggtgtc gacgcacggc tcgcgtgttc gaagtcggcc tgcgtttgat cgtcgctcgg cagcatcagc cccgggaaca ccgcgttcac gcgcagcacc ggcgccgacg aaagcgcgag catctgcgtg aggttgccga gcgccgcctt cgcgaccgtg tagctgaagt ggtcgcggtg gaagttctcc ttgatcttct gatcgaccac gttgaccacg acgccctgcg cgccggccgc gcgcgcccgc tcgtagaacg cacgcgtgag gaggatcggc gcgcggcagt tcacggccca cgcctgatcg aacgccgcga gttcgaagct cggaaaatgg tcctgccaga acaccgacgc attgttgacc agcacgtcga ggcggccgaa gcgcgcatac acctgatcga tcagcgcgct gatctgcgcg gcatccgaca ggtcggcctg cagcgcgact gaatcgtgcc cgcgttcggc gatcgcgcgg gcagccgtat gggcggcgtc ggccgagcga tcgtagtgga tcgcggtgcg gtagccgtga tcggcgaaat actcggcgaa tgcgcgcccg gcccggcgcg cggcgccggt cacgagcacg acgggcgtgt tcgccgcttg cttcaggggc tgcatatggt ggtggtggtg gtgagccatg gtata

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcagc ccctgaagca agcggcgaac acgcccgtcg tgctcgtgac cggcgccgcg cgccgggccg ggcgcgcatt cgccgagtat ttcgccgatc acggctaccg caccgcgatc cactacgatc gctcggccga cgccgcccat acggctgccc gcgcgatcgc cgaacgcggg cacgattcag tcgcgctgca ggccgacctg tcggatgccg cgcagatcag cgcgctgatc gatcaggtgt atgcgcgctt cggccgcctc gacgtgctgg tcaacaatgc gtcggtgttc tggcaggacc attttccgag cttcgaactc gcggcgttcg atcaggcgtg ggccgtgaac tgccgcgcgc cgatcctcct cacgcgtgcg ttctacgagc gggcgcgcgc ggccggcgcg cagggcgtcg tggtcaacgt ggtcgatcag aagatcaagg agaacttcca ccgcgaccac ttcagctaca cggtcgcgaa ggcggcgctc ggcaacctca cgcagatgct cgcgctttcg tcggcgccgg tgctgcgcgt gaacgcggtg ttcccggggc tgatgctgcc gagcgacgat caaacgcagg ccgacttcga acacgcgagc cgtgcgtcga caccgctcgc gcgcatcgcc ggccccgacg acgtggcgag cgcgatcctg ctgctcaccg gcagcgccta caacggcgtg gatttcgtcg tcgatgcggg gcagaacctg atccgcgtcg atcaggacgt gctgtacaag caccgatcgc cggccggcaa gcattgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat