BuviA.00010.x
Short-chain dehydrogenase/reductase SDR
| CENTER ID: | BuviA.00010.x |
| ORGANISM: | Burkholderia vietnamiensis G4 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuviA.00010.x.B1.GE39403 | Full length( BuviA.00010.x ) | 1 | 254 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuviA.00010.x.B1.PS02539 | Full length( BuviA.00010.x ) | 1 | 254 |
Structures
5IF3
DEPOSITED: 2/25/2016
DETERMINATION: XRay
CLONE: BuviA.00010.x.B1.GE39403
PROTEIN: BuviA.00010.x.B1.PS02539
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_131569 |
| PATRIC ID: | fig|269482.11.peg.2442 |
| RefSeq: | YP_001116179.1 |
| UniProt: | A4JKB1 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNATACAPRV RAIVTGHTRG LGASLAEQLL QQDIAVLGVS RSRHPSLAAT AGDRLVETEL DLSDTAAVAA WLAGGALRSF VDGASLVLLF NNAGVVDPIG PLAAQDPALV ARAVALNVAA PLMLSAALVQ AAAAPTECRV LHVSSGAARN AYAGWSVYCA TKAALDHHAR AVALDANRAL RICSVAPGVV DTGMQATIRS TSEANFPMRE KFDALKASGA LSTPDEAARH LIRYALSDAF GAEPTADVRN LPAG
NT Sequence
ATGAATGCAA CCGCTTGCGC ACCTCGCGTG CGCGCCATCG TCACCGGACA TACGCGCGGC CTCGGCGCAT CGCTCGCCGA ACAATTGCTG CAGCAGGACA TCGCCGTGCT CGGCGTATCG CGCAGCCGGC ATCCGTCGCT CGCCGCAACA GCCGGCGATC GCCTTGTCGA GACCGAGCTC GACCTGTCCG ACACCGCGGC CGTGGCGGCC TGGCTCGCCG GCGGCGCGCT GCGCAGCTTC GTCGACGGCG CATCGCTCGT CCTGCTGTTC AATAACGCAG GCGTCGTCGA TCCGATCGGC CCGCTGGCCG CGCAAGACCC GGCGCTGGTC GCGCGCGCGG TCGCGCTGAA CGTCGCCGCC CCGCTGATGC TGTCGGCCGC GCTCGTGCAA GCCGCAGCGG CGCCGACCGA ATGCCGCGTG CTGCACGTGT CGAGCGGCGC CGCCCGCAAC GCATACGCGG GCTGGAGCGT CTACTGCGCG ACGAAGGCCG CGCTCGATCA CCACGCGCGC GCCGTCGCGC TCGACGCGAA CCGCGCGCTG CGGATCTGCA GCGTCGCGCC GGGTGTCGTC GACACCGGCA TGCAGGCAAC GATCCGCTCG ACCAGCGAAG CCAACTTCCC GATGCGAGAA AAATTCGACG CGCTGAAAGC GAGCGGCGCG CTGTCGACGC CCGACGAGGC CGCGCGGCAT CTGATCCGCT ACGCGCTCAG CGACGCGTTC GGCGCCGAGC CGACGGCCGA CGTCCGCAAC CTGCCGGCCG GC
Details for BuviA.00010.x.B1.GE39403
| HARVESTED ON: | 8/5/2015 |
| SEQUENCED ON: | 8/13/2015 |
| EXPECTED MW: | 27kDa |
| OBSERVED MW: | 26kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMN ATACAPRVRA IVTGHTRGLG ASLAEQLLQQ DIAVLGVSRS RHPSLAATAG DRLVETELDL SDTAAVAAWL AGGALRSFVD GASLVLLFNN AGVVDPIGPL AAQDPALVAR AVALNVAAPL MLSAALVQAA AAPTECRVLH VSSGAARNAY AGWSVYCATK AALDHHARAV ALDANRALRI CSVAPGVVDT GMQATIRSTS EANFPMREKF DALKASGALS TPDEAARHLI RYALSDAFGA EPTADVRNLP AG
Validated NT Sequence
atcttactca cttagccggc cggcaggttg cggacgtcgg ccgtcggctc ggcgccgaac gcgtcgctga gcgcgtagcg gatcagatgc cgcgcggcct cgtcgggcgt cgacagcgcg ccgctcgctt tcagcgcgtc gaatttttct cgcatcggga agttggcttc gctggtcgag cggatcgttg cctgcatgcc ggtgtcgacg acacccggcg cgacgctgca gatccgcagc gcgcggttcg cgtcgagcgc gacggcgcgc gcgtggtgat cgagcgcggc cttcgtcgcg cagtagacgc tccagcccgc gtatgcgttg cgggcggcgc cgctcgacac gtgcagcacg cggcattcgg tcggcgccgc tgcggcttgc acgagcgcgg ccgacagcat cagcggggcg gcgacgttca gcgcgaccgc gcgcgcgacc agcgccgggt cttgcgcggc cagcgggccg atcggatcga cgacgcctgc gttattgaac agcaggacga gcgatgcgcc gtcgacgaag ctgcgcagcg cgccgccggc gagccaggcc gccacggccg cggtgtcgga caggtcgagc tcggtctcga caaggcgatc gccggctgtt gcggcgagcg acggatgccg gctgcgcgat acgccgagca cggcgatgtc ctgctgcagc aattgttcgg cgagcgatgc gccgaggccg cgcgtatgtc cggtgacgat ggcgcgcacg cgaggtgcgc aagcggttgc attcatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHMN ATACAPRVRA IVTGHTRGLG ASLAEQLLQQ DIAVLGVSRS RHPSLAATAG DRLVETELDL SDTAAVAAWL AGGALRSFVD GASLVLLFNN AGVVDPIGPL AAQDPALVAR AVALNVAAPL MLSAALVQAA AAPTECRVLH VSSGAARNAY AGWSVYCATK AALDHHARAV ALDANRALRI CSVAPGVVDT GMQATIRSTS EANFPMREKF DALKASGALS TPDEAARHLI RYALSDAFGA EPTADVRNLP AG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaatg caaccgcttg cgcacctcgc gtgcgcgcca tcgtcaccgg acatacgcgc ggcctcggcg catcgctcgc cgaacaattg ctgcagcagg acatcgccgt gctcggcgta tcgcgcagcc ggcatccgtc gctcgccgca acagccggcg atcgccttgt cgagaccgag ctcgacctgt ccgacaccgc ggccgtggcg gcctggctcg ccggcggcgc gctgcgcagc ttcgtcgacg gcgcatcgct cgtcctgctg ttcaataacg caggcgtcgt cgatccgatc ggcccgctgg ccgcgcaaga cccggcgctg gtcgcgcgcg cggtcgcgct gaacgtcgcc gccccgctga tgctgtcggc cgcgctcgtg caagccgcag cggcgccgac cgaatgccgc gtgctgcacg tgtcgagcgg cgccgcccgc aacgcatacg cgggctggag cgtctactgc gcgacgaagg ccgcgctcga tcaccacgcg cgcgccgtcg cgctcgacgc gaaccgcgcg ctgcggatct gcagcgtcgc gccgggtgtc gtcgacaccg gcatgcaggc aacgatccgc tcgaccagcg aagccaactt cccgatgcga gaaaaattcg acgcgctgaa agcgagcggc gcgctgtcga cgcccgacga ggccgcgcgg catctgatcc gctacgcgct cagcgacgcg ttcggcgccg agccgacggc cgacgtccgc aacctgccgg ccggctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for BuviA.00010.x.B1.PS02539
| PURIFICATION DATe: | 11/17/2015 |
| CONCENTRATION: | 60mg/ml |
| OBSERVED MW: | 27kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | SEC buffer |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMN ATACAPRVRA IVTGHTRGLG ASLAEQLLQQ DIAVLGVSRS RHPSLAATAG DRLVETELDL SDTAAVAAWL AGGALRSFVD GASLVLLFNN AGVVDPIGPL AAQDPALVAR AVALNVAAPL MLSAALVQAA AAPTECRVLH VSSGAARNAY AGWSVYCATK AALDHHARAV ALDANRALRI CSVAPGVVDT GMQATIRSTS EANFPMREKF DALKASGALS TPDEAARHLI RYALSDAFGA EPTADVRNLP AG
Validated NT Sequence
atcttactca cttagccggc cggcaggttg cggacgtcgg ccgtcggctc ggcgccgaac gcgtcgctga gcgcgtagcg gatcagatgc cgcgcggcct cgtcgggcgt cgacagcgcg ccgctcgctt tcagcgcgtc gaatttttct cgcatcggga agttggcttc gctggtcgag cggatcgttg cctgcatgcc ggtgtcgacg acacccggcg cgacgctgca gatccgcagc gcgcggttcg cgtcgagcgc gacggcgcgc gcgtggtgat cgagcgcggc cttcgtcgcg cagtagacgc tccagcccgc gtatgcgttg cgggcggcgc cgctcgacac gtgcagcacg cggcattcgg tcggcgccgc tgcggcttgc acgagcgcgg ccgacagcat cagcggggcg gcgacgttca gcgcgaccgc gcgcgcgacc agcgccgggt cttgcgcggc cagcgggccg atcggatcga cgacgcctgc gttattgaac agcaggacga gcgatgcgcc gtcgacgaag ctgcgcagcg cgccgccggc gagccaggcc gccacggccg cggtgtcgga caggtcgagc tcggtctcga caaggcgatc gccggctgtt gcggcgagcg acggatgccg gctgcgcgat acgccgagca cggcgatgtc ctgctgcagc aattgttcgg cgagcgatgc gccgaggccg cgcgtatgtc cggtgacgat ggcgcgcacg cgaggtgcgc aagcggttgc attcatatgg tggtggtggt ggtgagccat
Expressed Protein Sequence
MAHHHHHHMN ATACAPRVRA IVTGHTRGLG ASLAEQLLQQ DIAVLGVSRS RHPSLAATAG DRLVETELDL SDTAAVAAWL AGGALRSFVD GASLVLLFNN AGVVDPIGPL AAQDPALVAR AVALNVAAPL MLSAALVQAA AAPTECRVLH VSSGAARNAY AGWSVYCATK AALDHHARAV ALDANRALRI CSVAPGVVDT GMQATIRSTS EANFPMREKF DALKASGALS TPDEAARHLI RYALSDAFGA EPTADVRNLP AG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAATG CAACCGCTTG CGCACCTCGC GTGCGCGCCA TCGTCACCGG ACATACGCGC GGCCTCGGCG CATCGCTCGC CGAACAATTG CTGCAGCAGG ACATCGCCGT GCTCGGCGTA TCGCGCAGCC GGCATCCGTC GCTCGCCGCA ACAGCCGGCG ATCGCCTTGT CGAGACCGAG CTCGACCTGT CCGACACCGC GGCCGTGGCG GCCTGGCTCG CCGGCGGCGC GCTGCGCAGC TTCGTCGACG GCGCATCGCT CGTCCTGCTG TTCAATAACG CAGGCGTCGT CGATCCGATC GGCCCGCTGG CCGCGCAAGA CCCGGCGCTG GTCGCGCGCG CGGTCGCGCT GAACGTCGCC GCCCCGCTGA TGCTGTCGGC CGCGCTCGTG CAAGCCGCAG CGGCGCCGAC CGAATGCCGC GTGCTGCACG TGTCGAGCGG CGCCGCCCGC AACGCATACG CGGGCTGGAG CGTCTACTGC GCGACGAAGG CCGCGCTCGA TCACCACGCG CGCGCCGTCG CGCTCGACGC GAACCGCGCG CTGCGGATCT GCAGCGTCGC GCCGGGTGTC GTCGACACCG GCATGCAGGC AACGATCCGC TCGACCAGCG AAGCCAACTT CCCGATGCGA GAAAAATTCG ACGCGCTGAA AGCGAGCGGC GCGCTGTCGA CGCCCGACGA GGCCGCGCGG CATCTGATCC GCTACGCGCT CAGCGACGCG TTCGGCGCCG AGCCGACGGC CGACGTCCGC AACCTGCCGG CCGGCTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT