BuviA.00010.k
Short-chain dehydrogenase/reductase SDR
| CENTER ID: | BuviA.00010.k |
| ORGANISM: | Burkholderia vietnamiensis G4 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuviA.00010.k.B1.GE39400 | Full length( BuviA.00010.k ) | 1 | 259 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuviA.00010.k.B1.PS02585 | Full length( BuviA.00010.k ) | 1 | 259 |
Structures
5IDX
DEPOSITED: 2/24/2016
DETERMINATION: XRay
CLONE: BuviA.00010.k.B1.GE39400
PROTEIN: BuviA.00010.k.B1.PS02585
5IDY
DEPOSITED: 2/24/2016
DETERMINATION: XRay
CLONE: BuviA.00010.k.B1.GE39400
PROTEIN: BuviA.00010.k.B1.PS02585
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_129079 |
| PATRIC ID: | fig|269482.11.peg.6621 |
| RefSeq: | YP_001120211.1 |
| UniProt: | A4JGH3 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDLGIAGKTA LVCAASKGLG RGCAEALAAE GVNLVIVART RDTLERTADE IRAASNVSVA TVACDITTPD GRAAALAACP QPDILVNNAG GPPPGDFRDF SHDDWIRALE SNMLTPIELI RATVDGMIAR GFGRIVNITS SAVKAPIDVL ALSNGARSGL TGFVAGLARK VVGQGVTINN LLPGLFDTDR IATTLAAAAN AQGVTVDELR ARRTRDIPAG RLGTRAEFGA ACAFLCSVHA GYITGQNWLL DGGAYPGTF
NT Sequence
ATGGATCTCG GCATCGCAGG AAAGACCGCG CTCGTCTGCG CGGCGAGTAA GGGCCTCGGG CGCGGCTGCG CGGAAGCGCT GGCCGCCGAA GGCGTGAACC TCGTGATCGT CGCGCGCACG CGCGACACGC TCGAGCGAAC CGCGGACGAG ATCCGCGCGG CGTCGAACGT GTCGGTTGCC ACGGTCGCGT GCGACATCAC GACGCCCGAC GGCCGCGCGG CCGCGCTCGC CGCGTGCCCG CAGCCGGACA TTCTCGTGAA CAATGCCGGC GGCCCGCCGC CCGGCGACTT CCGCGACTTC TCGCACGACG ACTGGATCCG CGCGCTCGAG TCGAACATGC TGACGCCGAT CGAGCTGATC CGCGCGACCG TCGACGGGAT GATCGCGCGC GGCTTCGGCC GCATCGTCAA CATCACCAGC TCGGCCGTGA AGGCGCCGAT CGACGTGCTC GCGCTGTCCA ACGGCGCGCG CTCGGGGCTG ACCGGCTTCG TCGCGGGCCT CGCGCGCAAG GTGGTCGGGC AGGGCGTGAC GATCAACAAC CTGCTGCCGG GGCTGTTCGA CACCGACCGG ATCGCGACCA CGCTTGCCGC GGCGGCGAAC GCGCAGGGCG TGACGGTCGA CGAGCTGCGC GCGCGCCGCA CGCGGGACAT CCCGGCCGGG CGGCTCGGCA CGCGCGCCGA ATTCGGCGCG GCATGCGCGT TCCTGTGCAG CGTGCATGCC GGCTACATCA CGGGCCAGAA CTGGCTGCTC GACGGCGGCG CCTATCCGGG CACGTTC
Details for BuviA.00010.k.B1.GE39400
| HARVESTED ON: | 8/5/2015 |
| SEQUENCED ON: | 8/13/2015 |
| EXPECTED MW: | 28kDa |
| OBSERVED MW: | 28kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMD LGIAGKTALV CAASKGLGRG CAEALAAEGV NLVIVARTRD TLERTADEIR AASNVSVATV ACDITTPDGR AAALAACPQP DILVNNAGGP PPGDFRDFSH DDWIRALESN MLTPIELIRA TVDGMIARGF GRIVNITSSA VKAPIDVLAL SNGARSGLTG FVAGLARKVV GQGVTINNLL PGLFDTDRIA TTLAAAANAQ GVTVDELRAR RTRDIPAGRL GTRAEFGAAC AFLCSVHAGY ITGQNWLLDX GAYPGTF
Validated NT Sequence
atcttactca cttagaacgt gcccggatag gcgccgcngt cgagcagcca gttctggccc gtgatgtagc cggcatgcac gctgcacagg aacgcgcatg ccgcgccgaa ttcggcgcgc gtgccgagcc gcccggccgg gatgtcccgc gtgcggcgcg cgcgcagctc gtcgaccgtc acgccctgcg cgttcgccgc cgcggcaagc gtggtcgcga tccggtcggt gtcgaacagc cccggcagca ggttgttgat cgtcacgccc tgcccgacca ccttgcgcgc gaggcccgcg acgaagccgg tcagccccga gcgcgcgccg ttggacagcg cgagcacgtc gatcggcgcc ttcacggccg agctggtgat gttgacgatg cggccgaagc cgcgcgcgat catcccgtcg acggtcgcgc ggatcagctc gatcggcgtc agcatgttcg actcgagcgc gcggatccag tcgtcgtgcg agaagtcgcg gaagtcgccg ggcggcgggc cgccggcatt gttcacgaga atgtccggct gcgggcacgc ggcgagcgcg gccgcgcggc cgtcgggcgt cgtgatgtcg cacgcgaccg tggcaaccga cacgttcgac gccgcgcgga tctcgtccgc ggttcgctcg agcgtgtcgc gcgtgcgcgc gacgatcacg aggttcacgc cttcggcggc cagcgcttcc gcgcagccgc gcccgaggcc cttactcgcc gcgcagacga gcgcggtctt tcctgcgatg ccgagatcca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHMD LGIAGKTALV CAASKGLGRG CAEALAAEGV NLVIVARTRD TLERTADEIR AASNVSVATV ACDITTPDGR AAALAACPQP DILVNNAGGP PPGDFRDFSH DDWIRALESN MLTPIELIRA TVDGMIARGF GRIVNITSSA VKAPIDVLAL SNGARSGLTG FVAGLARKVV GQGVTINNLL PGLFDTDRIA TTLAAAANAQ GVTVDELRAR RTRDIPAGRL GTRAEFGAAC AFLCSVHAGY ITGQNWLLDG GAYPGTF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggatc tcggcatcgc aggaaagacc gcgctcgtct gcgcggcgag taagggcctc gggcgcggct gcgcggaagc gctggccgcc gaaggcgtga acctcgtgat cgtcgcgcgc acgcgcgaca cgctcgagcg aaccgcggac gagatccgcg cggcgtcgaa cgtgtcggtt gccacggtcg cgtgcgacat cacgacgccc gacggccgcg cggccgcgct cgccgcgtgc ccgcagccgg acattctcgt gaacaatgcc ggcggcccgc cgcccggcga cttccgcgac ttctcgcacg acgactggat ccgcgcgctc gagtcgaaca tgctgacgcc gatcgagctg atccgcgcga ccgtcgacgg gatgatcgcg cgcggcttcg gccgcatcgt caacatcacc agctcggccg tgaaggcgcc gatcgacgtg ctcgcgctgt ccaacggcgc gcgctcgggg ctgaccggct tcgtcgcggg cctcgcgcgc aaggtggtcg ggcagggcgt gacgatcaac aacctgctgc cggggctgtt cgacaccgac cggatcgcga ccacgcttgc cgcggcggcg aacgcgcagg gcgtgacggt cgacgagctg cgcgcgcgcc gcacgcggga catcccggcc gggcggctcg gcacgcgcgc cgaattcggc gcggcatgcg cgttcctgtg cagcgtgcat gccggctaca tcacgggcca gaactggctg ctcgacggcg gcgcctatcc gggcacgttc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for BuviA.00010.k.B1.PS02585
| PURIFICATION DATe: | 1/5/2016 |
| CONCENTRATION: | 64.29mg/ml |
| OBSERVED MW: | 28kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | SEC buffer |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMD LGIAGKTALV CAASKGLGRG CAEALAAEGV NLVIVARTRD TLERTADEIR AASNVSVATV ACDITTPDGR AAALAACPQP DILVNNAGGP PPGDFRDFSH DDWIRALESN MLTPIELIRA TVDGMIARGF GRIVNITSSA VKAPIDVLAL SNGARSGLTG FVAGLARKVV GQGVTINNLL PGLFDTDRIA TTLAAAANAQ GVTVDELRAR RTRDIPAGRL GTRAEFGAAC AFLCSVHAGY ITGQNWLLDX GAYPGTF
Validated NT Sequence
atcttactca cttagaacgt gcccggatag gcgccgcngt cgagcagcca gttctggccc gtgatgtagc cggcatgcac gctgcacagg aacgcgcatg ccgcgccgaa ttcggcgcgc gtgccgagcc gcccggccgg gatgtcccgc gtgcggcgcg cgcgcagctc gtcgaccgtc acgccctgcg cgttcgccgc cgcggcaagc gtggtcgcga tccggtcggt gtcgaacagc cccggcagca ggttgttgat cgtcacgccc tgcccgacca ccttgcgcgc gaggcccgcg acgaagccgg tcagccccga gcgcgcgccg ttggacagcg cgagcacgtc gatcggcgcc ttcacggccg agctggtgat gttgacgatg cggccgaagc cgcgcgcgat catcccgtcg acggtcgcgc ggatcagctc gatcggcgtc agcatgttcg actcgagcgc gcggatccag tcgtcgtgcg agaagtcgcg gaagtcgccg ggcggcgggc cgccggcatt gttcacgaga atgtccggct gcgggcacgc ggcgagcgcg gccgcgcggc cgtcgggcgt cgtgatgtcg cacgcgaccg tggcaaccga cacgttcgac gccgcgcgga tctcgtccgc ggttcgctcg agcgtgtcgc gcgtgcgcgc gacgatcacg aggttcacgc cttcggcggc cagcgcttcc gcgcagccgc gcccgaggcc cttactcgcc gcgcagacga gcgcggtctt tcctgcgatg ccgagatcca tatggtggtg gtggtggtga gccat
Expressed Protein Sequence
MAHHHHHHMD LGIAGKTALV CAASKGLGRG CAEALAAEGV NLVIVARTRD TLERTADEIR AASNVSVATV ACDITTPDGR AAALAACPQP DILVNNAGGP PPGDFRDFSH DDWIRALESN MLTPIELIRA TVDGMIARGF GRIVNITSSA VKAPIDVLAL SNGARSGLTG FVAGLARKVV GQGVTINNLL PGLFDTDRIA TTLAAAANAQ GVTVDELRAR RTRDIPAGRL GTRAEFGAAC AFLCSVHAGY ITGQNWLLDG GAYPGTF
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGATC TCGGCATCGC AGGAAAGACC GCGCTCGTCT GCGCGGCGAG TAAGGGCCTC GGGCGCGGCT GCGCGGAAGC GCTGGCCGCC GAAGGCGTGA ACCTCGTGAT CGTCGCGCGC ACGCGCGACA CGCTCGAGCG AACCGCGGAC GAGATCCGCG CGGCGTCGAA CGTGTCGGTT GCCACGGTCG CGTGCGACAT CACGACGCCC GACGGCCGCG CGGCCGCGCT CGCCGCGTGC CCGCAGCCGG ACATTCTCGT GAACAATGCC GGCGGCCCGC CGCCCGGCGA CTTCCGCGAC TTCTCGCACG ACGACTGGAT CCGCGCGCTC GAGTCGAACA TGCTGACGCC GATCGAGCTG ATCCGCGCGA CCGTCGACGG GATGATCGCG CGCGGCTTCG GCCGCATCGT CAACATCACC AGCTCGGCCG TGAAGGCGCC GATCGACGTG CTCGCGCTGT CCAACGGCGC GCGCTCGGGG CTGACCGGCT TCGTCGCGGG CCTCGCGCGC AAGGTGGTCG GGCAGGGCGT GACGATCAAC AACCTGCTGC CGGGGCTGTT CGACACCGAC CGGATCGCGA CCACGCTTGC CGCGGCGGCG AACGCGCAGG GCGTGACGGT CGACGAGCTG CGCGCGCGCC GCACGCGGGA CATCCCGGCC GGGCGGCTCG GCACGCGCGC CGAATTCGGC GCGGCATGCG CGTTCCTGTG CAGCGTGCAT GCCGGCTACA TCACGGGCCA GAACTGGCTG CTCGACGGCG GCGCCTATCC GGGCACGTTC TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT