SSGCID: Target Page

BuviA.00010.g

Short-chain dehydrogenase/reductase SDR

CENTER ID:	BuviA.00010.g
ORGANISM:	Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuviA.00010.g.B2.GE39399	Mature protein( BuviA.00010.g )		28	237

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|269482.11.peg.5665
RefSeq:	YP_001119275.1
UniProt:	A4JDT7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNDSLSGKKV LVVGGSSGIG AAAAHAFAQR GAVVTIASRH PERAGTAAAP GAAVRGEALD ITDTAAVDAF CARAGRFDHV VISAAQTATG AVRTLALADA QAAMDSKFWG AYRVARSIDI APGGSLSFVS GFLSVRPSAS SVLQGAINAA LEALARGLAL ELAPVRVNTV SPGLIATPLW NKLAPDARDA MYAGAAQRLP ARRVGQAEDV ANAIVYLATT PFATGSTVLV DGGGAIA

NT Sequence

ATGAACGATT CACTCAGCGG CAAGAAAGTC CTCGTCGTCG GCGGCAGCTC CGGCATCGGC GCCGCGGCCG CGCATGCATT CGCGCAACGC GGCGCGGTCG TGACGATCGC CTCGCGCCAC CCCGAGCGGG CCGGCACGGC TGCCGCGCCA GGCGCTGCGG TGCGCGGCGA AGCGCTCGAC ATCACCGATA CGGCAGCCGT CGACGCGTTC TGCGCGCGCG CGGGGCGCTT CGACCATGTC GTGATTTCGG CCGCGCAAAC GGCGACGGGC GCGGTGCGCA CGTTGGCGCT CGCCGATGCG CAGGCCGCGA TGGACAGCAA GTTCTGGGGC GCCTATCGCG TCGCGCGTTC GATCGACATC GCGCCCGGCG GCTCGCTGTC GTTCGTGTCG GGGTTCCTGA GCGTGCGGCC GAGCGCGTCG TCGGTGCTGC AAGGCGCGAT CAACGCGGCG CTCGAGGCGC TGGCCCGCGG GCTGGCGCTC GAGCTGGCGC CGGTGCGCGT GAACACGGTG TCGCCGGGCC TGATCGCGAC GCCGCTGTGG AACAAGCTCG CGCCCGACGC ACGCGACGCC ATGTACGCCG GCGCCGCGCA ACGCCTGCCC GCACGCCGCG TGGGCCAGGC CGAGGACGTC GCGAACGCGA TCGTCTACCT CGCGACGACG CCGTTTGCGA CCGGCTCGAC GGTGCTGGTC GACGGCGGCG GTGCGATCGC G

Details for BuviA.00010.g.B2.GE39399

HARVESTED ON:	8/5/2015
SEQUENCED ON:	8/13/2015
EXPECTED MW:	22kDa
OBSERVED MW:	22kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMA QRGAVVTIAS RHPERAGTAA APGAAVRGEA LDITDTAAVD AFCARAGRFD HVVISAAQTA TGAVRTLALA DAQAAMDSKF WGAYRVARSI DIAPGGSLSF VSGFLSVRPS ASSVLQGAIN AALEALARGL ALELAPVRVN TVSPGLIATP LWNKLAPDAR DAMYAGAAQR LPARRVGQAE DVANAIVYLA TTPFATGSTV LVDGGGAIA

Validated NT Sequence

atcttactca cttacgcgat cgcaccgccg ccgtcgacca gcaccgtcga gccggtcgca aacggcgtcg tcgcgaggta gacgatcgcg ttcgcgacgt cctcggcctg gcccacgcgg cgtgcgggca ggcgttgcgc ggcgccggcg tacatggcgt cgcgtgcgtc gggcgcgagc ttgttccaca gcggcgtcgc gatcaggccc ggcgacaccg tgttcacgcg caccggcgcc agctcgagcg ccagcccgcg ggccagcgcc tcgagcgccg cgttgatcgc gccttgcagc accgacgacg cgctcggccg cacgctcagg aaccccgaca cgaacgacag cgagccgccg ggcgcgatgt cgatcgaacg cgcgacgcga taggcgcccc agaacttgct gtccatcgcg gcctgcgcat cggcgagcgc caacgtgcgc accgcgcccg tcgccgtttg cgcggccgaa atcacgacat ggtcgaagcg ccccgcgcgc gcgcagaacg cgtcgacggc tgccgtatcg gtgatgtcga gcgcttcgcc gcgcaccgca gcgcctggcg cggcagccgt gccggcccgc tcggggtggc gcgaggcgat cgtcacgacc gcgccgcgtt gcgccatatg gtggtggtgg tggtgagcca t

Expected Protein Sequence

MAHHHHHHAQ RGAVVTIASR HPERAGTAAA PGAAVRGEAL DITDTAAVDA FCARAGRFDH VVISAAQTAT GAVRTLALAD AQAAMDSKFW GAYRVARSID IAPGGSLSFV SGFLSVRPSA SSVLQGAINA ALEALARGLA LELAPVRVNT VSPGLIATPL WNKLAPDARD AMYAGAAQRL PARRVGQAED VANAIVYLAT TPFATGSTVL VDGGGAIA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcgcaac gcggcgcggt cgtgacgatc gcctcgcgcc accccgagcg ggccggcacg gctgccgcgc caggcgctgc ggtgcgcggc gaagcgctcg acatcaccga tacggcagcc gtcgacgcgt tctgcgcgcg cgcggggcgc ttcgaccatg tcgtgatttc ggccgcgcaa acggcgacgg gcgcggtgcg cacgttggcg ctcgccgatg cgcaggccgc gatggacagc aagttctggg gcgcctatcg cgtcgcgcgt tcgatcgaca tcgcgcccgg cggctcgctg tcgttcgtgt cggggttcct gagcgtgcgg ccgagcgcgt cgtcggtgct gcaaggcgcg atcaacgcgg cgctcgaggc gctggcccgc gggctggcgc tcgagctggc gccggtgcgc gtgaacacgg tgtcgccggg cctgatcgcg acgccgctgt ggaacaagct cgcgcccgac gcacgcgacg ccatgtacgc cggcgccgcg caacgcctgc ccgcacgccg cgtgggccag gccgaggacg tcgcgaacgc gatcgtctac ctcgcgacga cgccgtttgc gaccggctcg acggtgctgg tcgacggcgg cggtgcgatc gcgtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat