SSGCID: Target Page

BuviA.00009.a

Kynureninase

CENTER ID:	BuviA.00009.a
ORGANISM:	Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuviA.00009.a.B1.GE38074	Full length( BuviA.00009.a )		1	417

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuviA.00009.a.B1.PW37514	Full length( BuviA.00009.a )		1	417

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|269482.11.peg.6939
RefSeq:	YP_001120526.1
UniProt:	A4JHD8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MIKTREDALA LDRDDPLAAL RGQFALPDGV IYLDGNSLGA QPRASAARAQ QVIGAEWGEG LIRSWNTAGW FALPRRLGDK LATLIGGVAG ETVVTDTISI NLFKLLSAML RHQAERAPQR RVIVSERSNF PTDLYIAQGL IEQLGGGYEL RLIDDPAELP AALGDDTAVA MITHVNYRTG YMHDMPAVTQ LAHDAGALML WDLAHSAGAV PVDLNGARAD GAVGCTYKYL NGGPGSPAFV WVPHRHHAHF SQPLSGWWGH RAPFAMQPAF APDPGIARFL CGTQPIVSMS MVECGLDVFL QTDMQAIRRK SLALTDAFIA LVEARCAGLS LKLVTPRAHH QRGSQASFEH PHGYEVMQAL IARGVIGDYR EPDVLRFGFT PLYTRFVDVW DAVDTLRDIL ATDAWKAPEF AERGAVT

NT Sequence

ATGATCAAGA CCCGTGAAGA CGCGCTCGCC CTCGACCGCG ACGATCCGCT CGCCGCGCTG CGCGGCCAGT TCGCGCTGCC CGACGGCGTG ATCTATCTCG ACGGCAATTC GCTCGGCGCG CAGCCGCGCG CATCGGCCGC CCGCGCGCAA CAGGTGATCG GCGCCGAATG GGGCGAAGGC CTGATCCGCA GCTGGAACAC CGCCGGCTGG TTCGCGCTGC CGCGCCGCCT CGGCGACAAG CTCGCGACGC TGATCGGCGG CGTAGCCGGC GAAACGGTCG TGACCGACAC GATCTCGATC AACCTGTTCA AGCTGCTGTC TGCGATGCTG CGCCATCAGG CCGAGCGCGC GCCGCAGCGC CGCGTGATCG TGTCCGAGCG CTCGAATTTC CCGACCGACC TGTACATCGC GCAAGGGCTG ATCGAGCAGC TCGGCGGCGG CTACGAGCTG CGTCTGATCG ACGATCCGGC GGAATTGCCG GCCGCGCTCG GCGACGACAC GGCCGTCGCG ATGATCACGC ACGTGAACTA CCGCACCGGC TACATGCACG ACATGCCGGC CGTCACGCAG CTCGCGCACG ACGCGGGCGC GCTGATGCTG TGGGATCTCG CCCACTCCGC CGGCGCGGTG CCGGTCGACC TGAACGGCGC GCGTGCCGAC GGCGCGGTCG GCTGCACGTA CAAATACCTG AACGGCGGCC CCGGCTCGCC GGCGTTCGTG TGGGTGCCGC ATCGCCATCA CGCGCATTTC TCGCAGCCGC TGTCCGGCTG GTGGGGCCAC CGCGCGCCGT TCGCGATGCA GCCGGCGTTC GCGCCGGACC CGGGCATCGC ACGCTTTCTG TGCGGCACGC AGCCGATCGT GTCGATGTCG ATGGTCGAAT GCGGGCTCGA CGTGTTCCTG CAGACCGACA TGCAGGCGAT CCGCCGCAAG TCGCTGGCGC TGACCGACGC GTTCATCGCG CTCGTCGAAG CGCGCTGCGC GGGGCTGTCG CTGAAGCTCG TCACGCCGCG CGCGCATCAC CAGCGCGGCT CGCAGGCGAG CTTCGAGCAT CCGCACGGCT ATGAAGTCAT GCAGGCGCTG ATCGCGCGCG GCGTGATCGG CGACTACCGC GAGCCCGACG TGCTGCGCTT CGGCTTCACG CCGCTCTATA CGCGCTTCGT CGACGTCTGG GACGCCGTCG ACACGCTGCG CGACATCCTC GCCACCGACG CGTGGAAGGC GCCCGAGTTC GCCGAGCGCG GCGCGGTGAC C

Details for BuviA.00009.a.B1.GE38074

HARVESTED ON:	5/13/2014
SEQUENCED ON:	5/6/2014
EXPECTED MW:	46kDa
OBSERVED MW:	43kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	25
PERCENT COVERAGE:	58

Validated AA Sequence

MLEARLRAAL VMRARRDELQ RQPRAARFDE RDERVGQRQR LAADRLHVGL QEHVEPAFDH RHRHDRLRAA QKACDARVRR ERRLHRERRA VAPPAGQRLR EMRVMAMRHP HERRRAGAAV QVFVRAADRA VGTRAVQVDR HRAGGVGEIP QHQRARVVRE LRDGRHVVHV AGAVVHVRDH RDGRVVAERG RQFRXDRRSD AARSRRRAAR SALARCTGRS GNSSARTRSR GAAARARPDG AASQTAA

Validated NT Sequence

cgaactcggg cgccttccac gcgtcggtgg cgaggatgtc gcgcagcgtg tcgacggcgt cccagacgtc gacgaagcgc gtatagagcg gcgtgaagcc gaagcgcagc acgtcgggct cgcggtagtc gccgatcacg ccgcgcgcga tcagcgcctg catgacttca tagccgtgcg gatgctcgaa gctcgcctgc gagccgcgct ggtgatgcgc gcgcggcgtg acgagcttca gcgacagccc cgcgcagcgc gcttcgacga gcgcgatgaa cgcgtcggtc agcgccagcg acttgcggcg gatcgcctgc atgtcggtct gcaggaacac gtcgagcccg cattcgacca tcgacatcga cacgatcggc tgcgtgccgc acagaaagcg tgcgatgccc gggtccggcg cgaacgccgg ctgcatcgcg aacggcgcgc ggtggcccca ccagccggac agcggctgcg agaaatgcgc gtgatggcga tgcggcaccc acacgaacgc cggcgagccg gggccgccgt tcaggtattt gtacgtgcag ccgaccgcgc cgtcggcacg cgcgccgttc aggtcgaccg gcaccgcgcc ggcggagtgg gcgagatccc acagcatcag cgcgcccgcg tcgtgcgcga gctgcgtgac ggccggcatg tcgtgcatgt agccggtgcg gtagttcacg tgcgtgatca tcgcgacggc cgtgtcgtcg ccgagcgcgg ccggcaattc cgccnggatc gtcgatcaga cgcagctcgt agccgccgcc gagctgctcg atcagccctt gcgcgatgta caggtcggtc gggaaattcg agcgctcgga cacgatcacg cggcgctgcg gcgcgcgctc ggcctgatgg cgcagcatcg cagacagcag cttgaacagg ttgatcgaga tcgtgtcggt cacgaccgtt tcgccggcta cgccgccgat cagcgtcgcg agcttgtcgc cgaggcggcg cggcagcgcg aaccagccgg cggtgttcca gctgcggatc aggccttcgc cccattcggc gccgatcacc tgttgcgcgc gggcggccga tgcgcgcggc tgcgcgccga gcgaattgcc gtcgagatag atcacgccgt cgggcagcgc gaactggccg cgcagcgcgg cgagcggatc gtcgcggtcg agggcgagc

Expected Protein Sequence

MAHHHHHHMI KTREDALALD RDDPLAALRG QFALPDGVIY LDGNSLGAQP RASAARAQQV IGAEWGEGLI RSWNTAGWFA LPRRLGDKLA TLIGGVAGET VVTDTISINL FKLLSAMLRH QAERAPQRRV IVSERSNFPT DLYIAQGLIE QLGGGYELRL IDDPAELPAA LGDDTAVAMI THVNYRTGYM HDMPAVTQLA HDAGALMLWD LAHSAGAVPV DLNGARADGA VGCTYKYLNG GPGSPAFVWV PHRHHAHFSQ PLSGWWGHRA PFAMQPAFAP DPGIARFLCG TQPIVSMSMV ECGLDVFLQT DMQAIRRKSL ALTDAFIALV EARCAGLSLK LVTPRAHHQR GSQASFEHPH GYEVMQALIA RGVIGDYREP DVLRFGFTPL YTRFVDVWDA VDTLRDILAT DAWKAPEFAE RGAVT

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatca agacccgtga agacgcgctc gccctcgacc gcgacgatcc gctcgccgcg ctgcgcggcc agttcgcgct gcccgacggc gtgatctatc tcgacggcaa ttcgctcggc gcgcagccgc gcgcatcggc cgcccgcgcg caacaggtga tcggcgccga atggggcgaa ggcctgatcc gcagctggaa caccgccggc tggttcgcgc tgccgcgccg cctcggcgac aagctcgcga cgctgatcgg cggcgtagcc ggcgaaacgg tcgtgaccga cacgatctcg atcaacctgt tcaagctgct gtctgcgatg ctgcgccatc aggccgagcg cgcgccgcag cgccgcgtga tcgtgtccga gcgctcgaat ttcccgaccg acctgtacat cgcgcaaggg ctgatcgagc agctcggcgg cggctacgag ctgcgtctga tcgacgatcc ggcggaattg ccggccgcgc tcggcgacga cacggccgtc gcgatgatca cgcacgtgaa ctaccgcacc ggctacatgc acgacatgcc ggccgtcacg cagctcgcgc acgacgcggg cgcgctgatg ctgtgggatc tcgcccactc cgccggcgcg gtgccggtcg acctgaacgg cgcgcgtgcc gacggcgcgg tcggctgcac gtacaaatac ctgaacggcg gccccggctc gccggcgttc gtgtgggtgc cgcatcgcca tcacgcgcat ttctcgcagc cgctgtccgg ctggtggggc caccgcgcgc cgttcgcgat gcagccggcg ttcgcgccgg acccgggcat cgcacgcttt ctgtgcggca cgcagccgat cgtgtcgatg tcgatggtcg aatgcgggct cgacgtgttc ctgcagaccg acatgcaggc gatccgccgc aagtcgctgg cgctgaccga cgcgttcatc gcgctcgtcg aagcgcgctg cgcggggctg tcgctgaagc tcgtcacgcc gcgcgcgcat caccagcgcg gctcgcaggc gagcttcgag catccgcacg gctatgaagt catgcaggcg ctgatcgcgc gcggcgtgat cggcgactac cgcgagcccg acgtgctgcg cttcggcttc acgccgctct atacgcgctt cgtcgacgtc tgggacgccg tcgacacgct gcgcgacatc ctcgccaccg acgcgtggaa ggcgcccgag ttcgccgagc gcggcgcggt gacctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for BuviA.00009.a.B1.PW37514

PURIFICATION DATe:	7/2/2014
CONCENTRATION:	29.8mg/ml
OBSERVED MW:	47kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	14
VIAL VOLUME:	200µl
PERCENT IDENTITY:	25
PERCENT COVERAGE:	58

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGATCA AGACCCGTGA AGACGCGCTC GCCCTCGACC GCGACGATCC GCTCGCCGCG CTGCGCGGCC AGTTCGCGCT GCCCGACGGC GTGATCTATC TCGACGGCAA TTCGCTCGGC GCGCAGCCGC GCGCATCGGC CGCCCGCGCG CAACAGGTGA TCGGCGCCGA ATGGGGCGAA GGCCTGATCC GCAGCTGGAA CACCGCCGGC TGGTTCGCGC TGCCGCGCCG CCTCGGCGAC AAGCTCGCGA CGCTGATCGG CGGCGTAGCC GGCGAAACGG TCGTGACCGA CACGATCTCG ATCAACCTGT TCAAGCTGCT GTCTGCGATG CTGCGCCATC AGGCCGAGCG CGCGCCGCAG CGCCGCGTGA TCGTGTCCGA GCGCTCGAAT TTCCCGACCG ACCTGTACAT CGCGCAAGGG CTGATCGAGC AGCTCGGCGG CGGCTACGAG CTGCGTCTGA TCGACGATCC GGCGGAATTG CCGGCCGCGC TCGGCGACGA CACGGCCGTC GCGATGATCA CGCACGTGAA CTACCGCACC GGCTACATGC ACGACATGCC GGCCGTCACG CAGCTCGCGC ACGACGCGGG CGCGCTGATG CTGTGGGATC TCGCCCACTC CGCCGGCGCG GTGCCGGTCG ACCTGAACGG CGCGCGTGCC GACGGCGCGG TCGGCTGCAC GTACAAATAC CTGAACGGCG GCCCCGGCTC GCCGGCGTTC GTGTGGGTGC CGCATCGCCA TCACGCGCAT TTCTCGCAGC CGCTGTCCGG CTGGTGGGGC CACCGCGCGC CGTTCGCGAT GCAGCCGGCG TTCGCGCCGG ACCCGGGCAT CGCACGCTTT CTGTGCGGCA CGCAGCCGAT CGTGTCGATG TCGATGGTCG AATGCGGGCT CGACGTGTTC CTGCAGACCG ACATGCAGGC GATCCGCCGC AAGTCGCTGG CGCTGACCGA CGCGTTCATC GCGCTCGTCG AAGCGCGCTG CGCGGGGCTG TCGCTGAAGC TCGTCACGCC GCGCGCGCAT CACCAGCGCG GCTCGCAGGC GAGCTTCGAG CATCCGCACG GCTATGAAGT CATGCAGGCG CTGATCGCGC GCGGCGTGAT CGGCGACTAC CGCGAGCCCG ACGTGCTGCG CTTCGGCTTC ACGCCGCTCT ATACGCGCTT CGTCGACGTC TGGGACGCCG TCGACACGCT GCGCGACATC CTCGCCACCG ACGCGTGGAA GGCGCCCGAG TTCGCCGAGC GCGGCGCGGT GACCTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT