ButhA.18065.a
Putative deoxyribonuclease-2
| CENTER ID: | ButhA.18065.a |
| ORGANISM: | Burkholderia thailandensis E264 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.18065.a.B1.GE35619 | full length | 1 | 350 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.18065.a.EM12.PD00321 | full length | 1 | 351 | unavailable | |
| ButhA.18065.a.B1.PS01766 | full length | 1 | 350 |
Structures
5I3E
DEPOSITED: 2/10/2016
DETERMINATION: XRay
CLONE: ButhA.18065.a.B1.GE35619
PROTEIN: ButhA.18065.a.B1.PS01811
5UNB
DEPOSITED: 1/30/2017
DETERMINATION: XRay
CLONE: ButhA.18065.a.B1.GE35619
PROTEIN: ButhA.18065.a.B1.PS01811
Publications by SSGCID
Structure of acid deoxyribonuclease
Abendroth J, Edwards TE, Lorimer D, Phan IQ, Aguilera R, Varela-Ramirez A, Mejia AA
Nucleic Acids Res. - 2017
volume 45, issue 10, pages 6217-6227
PMID: 28369538; PMCID: PMC5449587
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_130788 |
| PATRIC ID: | fig|271848.6.peg.432 |
| RefSeq: | YP_438589.1 |
| UniProt: | Q2T8B0 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAISPRDEQN RSVDLWFAYK VPKLTKDADS DSASGYEYVY YDRQVGAVQK SPNLMNDPKG ALFYTLDSVF GDPGDTTGWI LYNDEMPADA NRSNNATLGH TKGVIAFDIA SSSALWLLHS WPKYASPSVP GVPTPLYGQT FLCLSLDLAT AGKLAAQMAL HQQPQVYLPR TGGLDHTSPL YALTQPLNAS APGDSDSLDF KTRGGVPFKV IAKNRKWGKD FWNDLVGPTL KADMYVETWI RGKIPPVLDS DGVHKTYDIK FIDLRKLGAP WAWPETQDHA KWGITTTDNW VCVGDINRMV TQEKRGGGTI AFQDPKLWKA LCETDLIIPP PGKTDAQARA MIRKTHEPAE
NT Sequence
atggccatct cgccgcgcga cgaacagaat cgatccgtcg atctgtggtt cgcctacaaa gtaccgaagc tcacgaagga tgccgacagc gacagcgcga gcggctacga gtacgtgtac tacgaccgtc aagtcggggc agtgcagaaa tcgcctaacc tgatgaatga tccgaagggg gcgctgttct acacgctcga ttcggtcttc ggcgatccgg gcgacacgac gggctggatt ctctacaacg acgaaatgcc cgccgatgcg aatcgctcga acaatgcgac gctcggccat acgaaaggcg tgatcgcctt cgatatcgcg tcgagctccg cattgtggct gctgcactcg tggccgaaat acgcgagccc gtcggtgccc ggcgtgccga cgccgcttta cggccagaca ttcctgtgcc tgagcctcga tctcgcgacg gccggcaagc tcgccgcgca gatggcgctg catcagcagc cgcaggtcta tctgccgcgc accggcgggc tcgatcacac gagccccctg tatgcgctca ctcaaccgct caacgcatcc gcgcccggag attccgactc gctcgatttc aagacgcgcg gcggcgtgcc gttcaaggtg atcgcgaaga atcgcaaatg gggaaaggac ttctggaacg acctcgtcgg cccgacactg aaagccgaca tgtacgtcga gacctggata cgcggcaaga tcccgcccgt cctcgattcg gacggcgtgc acaagaccta cgacatcaag ttcatcgatc tgcgcaagct gggcgcgccg tgggcgtggc ccgagacgca ggaccacgcg aagtggggca tcacgacgac cgacaactgg gtatgcgtcg gcgacatcaa ccggatggtg acgcaagaga aacgcggcgg cggcacgatc gccttccagg acccgaagct gtggaaggcg ctttgcgaaa ccgatctgat cataccgccg cccggcaaga ccgacgcgca ggcacgggca atgatccgca agacgcacga accggccgaa tag
Details for ButhA.18065.a.B1.GE35619
| HARVESTED ON: | 6/12/2012 |
| SEQUENCED ON: | 6/12/2012 |
| EXPECTED MW: | 39kDa |
| OBSERVED MW: | 39kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 95 |
Validated AA Sequence
MAISPRDEQN RSVDLWFAYK VPKLTKDADS DSASGYEYVY YDRQVGAVQK SPNLMNDPKG ALFYTLDSVF GDPGDTTGWI LYNDEMPADA NRSNNATLGH TKGVIAFDIA SSSALWLLHS WPKYASPSVP GVPTPLYGQT FLCLSLDLAT AGKLAAQMAL HQQPQVYLPR TGGLDHTSPL YALTQPLNAS APGDSDSLDF KTRGGVPFKV IAKNRKWGKD FWNDLVGPTL KADMYVETWI RGKIPPVLDS DGVHKTYDIK FIDLRKLGAP WAWPETQDHA KWGITTTDNW VCVGDINRMV TQEKRGGGTI AFQDPKLWKA LCETDLIIPP PGKTDAQARA M
Validated NT Sequence
gatcattgcc cgtgcctgcg cgtcggtctt gccgggcggc ggtatgatca gatcggtttc gcaaagcgcc ttccacagct tcgggtcctg gaaggcgatc gtgccgccgc cgcgtttctc ttgcgtcacc atccggttga tgtcgccgac gcatacccag ttgtcggtcg tcgtgatgcc ccacttcgcg tggtcctgcg tctcgggcca cgcccacggc gcgcccagct tgcgcagatc gatgaacttg atgtcgtagg tcttgtgcac gccgtccgaa tcgaggacgg gcgggatctt gccgcgtatc caggtctcga cgtacatgtc ggctttcagt gtcgggccga cgaggtcgtt ccagaagtcc tttccccatt tgcgattctt cgcgatcacc ttgaacggca cgccgccgcg cgtcttgaaa tcgagcgagt cggaatctcc gggcgcggat gcgttgagcg gttgagtgag cgcatacagg gggctcgtgt gatcgagccc gccggtgcgc ggcagataga cctgcggctg ctgatgcagc gccatctgcg cggcgagctt gccggccgtc gcgagatcga ggctcaggca caggaatgtc tggccgtaaa gcggcgtcgg cacgccgggc accgacgggc tcgcgtattt cggccacgag tgcagcagcc acaatgcgga gctcgacgcg atatcgaagg cgatcacgcc tttcgtatgg ccgagcgtcg cattgttcga gcgattcgca tcggcgggca tttcgtcgtt gtagagaatc cagcccgtcg tgtcgcccgg atcgccgaag accgaatcga gcgtgtagaa cagcgccccc ttcggatcat tcatcaggtt aggcgatttc tgcactgccc cgacttgacg gtcgtagtac acgtactcgt agccgctcgc gctgtcgctg tcggcatcct tcgtgagctt cggtactttg taggcgaacc acagatcgac ggatcgattc tgttcgtcgc gcggcgagat ggccatatgg tgg
Expected Protein Sequence
MAHHHHHHMA ISPRDEQNRS VDLWFAYKVP KLTKDADSDS ASGYEYVYYD RQVGAVQKSP NLMNDPKGAL FYTLDSVFGD PGDTTGWILY NDEMPADANR SNNATLGHTK GVIAFDIASS SALWLLHSWP KYASPSVPGV PTPLYGQTFL CLSLDLATAG KLAAQMALHQ QPQVYLPRTG GLDHTSPLYA LTQPLNASAP GDSDSLDFKT RGGVPFKVIA KNRKWGKDFW NDLVGPTLKA DMYVETWIRG KIPPVLDSDG VHKTYDIKFI DLRKLGAPWA WPETQDHAKW GITTTDNWVC VGDINRMVTQ EKRGGGTIAF QDPKLWKALC ETDLIIPPPG KTDAQARAMI RKTHEPAE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcca tctcgccgcg cgacgaacag aatcgatccg tcgatctgtg gttcgcctac aaagtaccga agctcacgaa ggatgccgac agcgacagcg cgagcggcta cgagtacgtg tactacgacc gtcaagtcgg ggcagtgcag aaatcgccta acctgatgaa tgatccgaag ggggcgctgt tctacacgct cgattcggtc ttcggcgatc cgggcgacac gacgggctgg attctctaca acgacgaaat gcccgccgat gcgaatcgct cgaacaatgc gacgctcggc catacgaaag gcgtgatcgc cttcgatatc gcgtcgagct ccgcattgtg gctgctgcac tcgtggccga aatacgcgag cccgtcggtg cccggcgtgc cgacgccgct ttacggccag acattcctgt gcctgagcct cgatctcgcg acggccggca agctcgccgc gcagatggcg ctgcatcagc agccgcaggt ctatctgccg cgcaccggcg ggctcgatca cacgagcccc ctgtatgcgc tcactcaacc gctcaacgca tccgcgcccg gagattccga ctcgctcgat ttcaagacgc gcggcggcgt gccgttcaag gtgatcgcga agaatcgcaa atggggaaag gacttctgga acgacctcgt cggcccgaca ctgaaagccg acatgtacgt cgagacctgg atacgcggca agatcccgcc cgtcctcgat tcggacggcg tgcacaagac ctacgacatc aagttcatcg atctgcgcaa gctgggcgcg ccgtgggcgt ggcccgagac gcaggaccac gcgaagtggg gcatcacgac gaccgacaac tgggtatgcg tcggcgacat caaccggatg gtgacgcaag agaaacgcgg cggcggcacg atcgccttcc aggacccgaa gctgtggaag gcgctttgcg aaaccgatct gatcataccg ccgcccggca agaccgacgc gcaggcacgg gcaatgatcc gcaagacgca cgaaccggcc gaatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for ButhA.18065.a.EM12.PD00321
| PURIFICATION DATe: | 5/14/2014 |
| CONCENTRATION: | 19.9mg/ml |
| OBSERVED MW: | 42kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20mM Tris-HCl, pH 8.0, 200mM NaCl, 1% glycerol, 1mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | data unavailable |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMA ISPRDEQNRS VDLWFAYKVP KLTKDADSDS ASGYEYVYYD RQVGAVQKSP NLMNDPKGAL FYTLDSVFGD PGDTTGWILY NDEMPADANR SNNATLGHTK GVIAFDIASS SALWLLHSWP KYASPSVPGV PTPLYGQTFL CLSLDLATAG KLAAQMALHQ QPQVYLPRTG GLDHTSPLYA LTQPLNASAP GDSDSLDFKT RGGVPFKVIA KNRKWGKDFW NDLVGPTLKA DMYVETWIRG KIPPVLDSDG VHKTYDIKFI DLRKLGAPWA WPETQDAAKW GITTTDNWVC VGDINRMVTQ EKRGGGTIAF QDPKLWKALC ETDLIIPPPG KTDAQARAMI RKTHEPAE
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCCA TCTCGCCGCG CGACGAACAG AATCGATCCG TCGATCTGTG GTTCGCCTAC AAAGTACCGA AGCTCACGAA GGATGCCGAC AGCGACAGCG CGAGCGGCTA CGAGTACGTG TACTACGACC GTCAAGTCGG GGCAGTGCAG AAATCGCCTA ACCTGATGAA TGATCCGAAG GGGGCGCTGT TCTACACGCT CGATTCGGTC TTCGGCGATC CGGGCGACAC GACGGGCTGG ATTCTCTACA ACGACGAAAT GCCCGCCGAT GCGAATCGCT CGAACAATGC GACGCTCGGC CATACGAAAG GCGTGATCGC CTTCGATATC GCGTCGAGCT CCGCATTGTG GCTGCTGCAC TCGTGGCCGA AATACGCGAG CCCGTCGGTG CCCGGCGTGC CGACGCCGCT TTACGGCCAG ACATTCCTGT GCCTGAGCCT CGATCTCGCG ACGGCCGGCA AGCTCGCCGC GCAGATGGCG CTGCATCAGC AGCCGCAGGT CTATCTGCCG CGCACCGGCG GGCTCGATCA CACGAGCCCC CTGTATGCGC TCACTCAACC GCTCAACGCA TCCGCGCCCG GAGATTCCGA CTCGCTCGAT TTCAAGACGC GCGGCGGCGT GCCGTTCAAG GTGATCGCGA AGAATCGCAA ATGGGGAAAG GACTTCTGGA ACGACCTCGT CGGCCCGACA CTGAAAGCCG ACATGTACGT CGAGACCTGG ATACGCGGCA AGATCCCGCC CGTCCTCGAT TCGGACGGCG TGCACAAGAC CTACGACATC AAGTTCATCG ATCTGCGCAA GCTGGGCGCG CCGTGGGCGT GGCCCGAGAC GCAGGACGCG GCGAAGTGGG GCATCACGAC GACCGACAAC TGGGTATGCG TCGGCGACAT CAACCGGATG GTGACGCAAG AGAAACGCGG CGGCGGCACG ATCGCCTTCC AGGACCCGAA GCTGTGGAAG GCGCTTTGCG AAACCGATCT GATCATACCG CCGCCCGGCA AGACCGACGC GCAGGCACGG GCAATGATCC GCAAGACGCA CGAACCGGCC GAATAAGTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT
Details for ButhA.18065.a.B1.PS01766
| PURIFICATION DATe: | 3/17/2013 |
| CONCENTRATION: | 28mg/ml |
| OBSERVED MW: | 36kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 95 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAISPRDEQN RSVDLWFAYK VPKLTKDADS DSASGYEYVY YDRQVGAVQK SPNLMNDPKG ALFYTLDSVF GDPGDTTGWI LYNDEMPADA NRSNNATLGH TKGVIAFDIA SSSALWLLHS WPKYASPSVP GVPTPLYGQT FLCLSLDLAT AGKLAAQMAL HQQPQVYLPR TGGLDHTSPL YALTQPLNAS APGDSDSLDF KTRGGVPFKV IAKNRKWGKD FWNDLVGPTL KADMYVETWI RGKIPPVLDS DGVHKTYDIK FIDLRKLGAP WAWPETQDHA KWGITTTDNW VCVGDINRMV TQEKRGGGTI AFQDPKLWKA LCETDLIIPP PGKTDAQARA M
Validated NT Sequence
gatcattgcc cgtgcctgcg cgtcggtctt gccgggcggc ggtatgatca gatcggtttc gcaaagcgcc ttccacagct tcgggtcctg gaaggcgatc gtgccgccgc cgcgtttctc ttgcgtcacc atccggttga tgtcgccgac gcatacccag ttgtcggtcg tcgtgatgcc ccacttcgcg tggtcctgcg tctcgggcca cgcccacggc gcgcccagct tgcgcagatc gatgaacttg atgtcgtagg tcttgtgcac gccgtccgaa tcgaggacgg gcgggatctt gccgcgtatc caggtctcga cgtacatgtc ggctttcagt gtcgggccga cgaggtcgtt ccagaagtcc tttccccatt tgcgattctt cgcgatcacc ttgaacggca cgccgccgcg cgtcttgaaa tcgagcgagt cggaatctcc gggcgcggat gcgttgagcg gttgagtgag cgcatacagg gggctcgtgt gatcgagccc gccggtgcgc ggcagataga cctgcggctg ctgatgcagc gccatctgcg cggcgagctt gccggccgtc gcgagatcga ggctcaggca caggaatgtc tggccgtaaa gcggcgtcgg cacgccgggc accgacgggc tcgcgtattt cggccacgag tgcagcagcc acaatgcgga gctcgacgcg atatcgaagg cgatcacgcc tttcgtatgg ccgagcgtcg cattgttcga gcgattcgca tcggcgggca tttcgtcgtt gtagagaatc cagcccgtcg tgtcgcccgg atcgccgaag accgaatcga gcgtgtagaa cagcgccccc ttcggatcat tcatcaggtt aggcgatttc tgcactgccc cgacttgacg gtcgtagtac acgtactcgt agccgctcgc gctgtcgctg tcggcatcct tcgtgagctt cggtactttg taggcgaacc acagatcgac ggatcgattc tgttcgtcgc gcggcgagat ggccatatgg tgg
Expressed Protein Sequence
MAHHHHHHMA ISPRDEQNRS VDLWFAYKVP KLTKDADSDS ASGYEYVYYD RQVGAVQKSP NLMNDPKGAL FYTLDSVFGD PGDTTGWILY NDEMPADANR SNNATLGHTK GVIAFDIASS SALWLLHSWP KYASPSVPGV PTPLYGQTFL CLSLDLATAG KLAAQMALHQ QPQVYLPRTG GLDHTSPLYA LTQPLNASAP GDSDSLDFKT RGGVPFKVIA KNRKWGKDFW NDLVGPTLKA DMYVETWIRG KIPPVLDSDG VHKTYDIKFI DLRKLGAPWA WPETQDHAKW GITTTDNWVC VGDINRMVTQ EKRGGGTIAF QDPKLWKALC ETDLIIPPPG KTDAQARAMI RKTHEPAE
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCCA TCTCGCCGCG CGACGAACAG AATCGATCCG TCGATCTGTG GTTCGCCTAC AAAGTACCGA AGCTCACGAA GGATGCCGAC AGCGACAGCG CGAGCGGCTA CGAGTACGTG TACTACGACC GTCAAGTCGG GGCAGTGCAG AAATCGCCTA ACCTGATGAA TGATCCGAAG GGGGCGCTGT TCTACACGCT CGATTCGGTC TTCGGCGATC CGGGCGACAC GACGGGCTGG ATTCTCTACA ACGACGAAAT GCCCGCCGAT GCGAATCGCT CGAACAATGC GACGCTCGGC CATACGAAAG GCGTGATCGC CTTCGATATC GCGTCGAGCT CCGCATTGTG GCTGCTGCAC TCGTGGCCGA AATACGCGAG CCCGTCGGTG CCCGGCGTGC CGACGCCGCT TTACGGCCAG ACATTCCTGT GCCTGAGCCT CGATCTCGCG ACGGCCGGCA AGCTCGCCGC GCAGATGGCG CTGCATCAGC AGCCGCAGGT CTATCTGCCG CGCACCGGCG GGCTCGATCA CACGAGCCCC CTGTATGCGC TCACTCAACC GCTCAACGCA TCCGCGCCCG GAGATTCCGA CTCGCTCGAT TTCAAGACGC GCGGCGGCGT GCCGTTCAAG GTGATCGCGA AGAATCGCAA ATGGGGAAAG GACTTCTGGA ACGACCTCGT CGGCCCGACA CTGAAAGCCG ACATGTACGT CGAGACCTGG ATACGCGGCA AGATCCCGCC CGTCCTCGAT TCGGACGGCG TGCACAAGAC CTACGACATC AAGTTCATCG ATCTGCGCAA GCTGGGCGCG CCGTGGGCGT GGCCCGAGAC GCAGGACCAC GCGAAGTGGG GCATCACGAC GACCGACAAC TGGGTATGCG TCGGCGACAT CAACCGGATG GTGACGCAAG AGAAACGCGG CGGCGGCACG ATCGCCTTCC AGGACCCGAA GCTGTGGAAG GCGCTTTGCG AAACCGATCT GATCATACCG CCGCCCGGCA AGACCGACGC GCAGGCACGG GCAATGATCC GCAAGACGCA CGAACCGGCC GAATGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT