ButhA.18004.a

Succinate dehydrogenase, flavoprotein subunit (BTH_II0662) (sdhA)

CENTER ID: ButhA.18004.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.18004.a.A1.GE32974 full length 1 591
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.18004.a.A1.PS01241 full length 1 591

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.747
RefSeq: YP_438861.1
UniProt: Q2T7I8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAAIKTSLPR RKFDVVIVGA GGSGMRASLQ LSRAGLSVCV LSKVFPTRSH TVAAQGGIGA SLGNMSEDNW HYHFYDTIKG SDWLGDQDAI EFMCREAPNV VYELEHFGMP FDRNADGTIY QRPFGGHTAN YGEKPVQRAC AAADRTGHAL LHTLYQQNVA AKTQFFVEWM ALDLIRDADG DVLGVTALEM ETGDVYILEG KTTLFATGGA GRIFAASTNA FINTGDGLGM AARSGIALQD MEFWQFHPTG VAGAGVLITE GVRGEGGILR NANGERFMER YAPTLKDLAP RDFVSRSMDQ EIKEGRGVGP NKDHVLLDLS HIGAETIMKR LPSIREIALK FANVDCIKEP IPVVPTIHYQ MGGIPTNIHG QVVGTSRDHK EPINGFYAVG ECSCVSVHGA NRLGTNSLLD LVVFGRAAGN HIVEHVKNQK EHKPLPADAA EFSLSRLAKL EKSTSGEYTQ DIANDIRATM QKHAGVFRTS ALLKEGVEQM AGLKERAASV HLKDKSKVFN TARVEALELA NLIEVARATM VSAEARKESR GAHAHSDYEH RDDENWLRHT LWYSEGDRLD YKPVQMKPLT VESVPPKART F
NT Sequence
atggctgcaa tcaaaacttc cctgccgcgt cgcaagttcg acgtggtgat cgtcggcgcg ggcggctcgg ggatgcgcgc gtcgctgcaa ctgtcgcgcg cgggcctctc cgtatgcgtg ctgtcgaagg tgttcccgac ccgttcgcac acggtcgccg cgcaaggcgg gatcggcgcg tcgctcggca acatgagcga agacaactgg cactaccact tctacgacac gatcaagggc tccgactggc tcggcgacca ggacgcgatc gagttcatgt gccgcgaagc gccgaacgtc gtgtacgagc tcgagcactt cggcatgccg ttcgaccgta acgcggacgg cacgatctac cagcgtccgt tcggcggcca cacggccaac tacggcgaga agccggttca gcgcgcgtgc gcggcggccg accgcaccgg ccacgcgctg ctgcacacgc tgtaccagca gaacgtcgcg gcgaagacgc agttcttcgt cgaatggatg gcgctcgacc tgatccgcga cgcggacggc gacgtgctcg gcgtgacggc cctcgaaatg gaaacgggcg acgtctacat cctcgaaggc aagaccacgc tgttcgccac gggcggcgcg ggccggatct tcgcggcgtc gaccaacgcg ttcatcaaca ccggcgacgg cctcggcatg gcggcccgct cgggcatcgc gctgcaggac atggagttct ggcaattcca cccgacgggc gtcgcgggcg cgggcgtgct gatcaccgaa ggcgtgcgcg gcgaaggcgg cattctgcgc aacgcgaacg gcgagcgctt catggagcgc tacgcgccga cgctgaagga tctggcgccg cgcgatttcg tgtcgcgctc gatggaccag gaaatcaagg aaggccgcgg cgtgggcccg aacaaggatc acgtgctgct cgacctgtcg cacatcggcg cggagacgat catgaagcgt ctgccgtcga tccgcgaaat cgcgctgaag ttcgcgaacg tcgactgcat caaggaaccg attcccgtcg tgccgacgat ccactaccag atgggcggca tcccgaccaa catccacggc caggtggtgg gcacgtcgcg cgatcacaag gagccgatca acggcttcta cgcggtgggc gaatgctcgt gcgtgtccgt gcacggcgcg aaccgtctcg gcacgaactc gctgctcgac ctcgtcgtgt tcggccgcgc ggccggcaac cacatcgtcg agcacgtgaa gaaccagaag gagcacaagc cgctgccggc cgacgccgcc gaattctcgc tgtcgcgcct cgcgaagctc gagaagtcga cgtcgggcga gtacacgcag gacatcgcga acgacatccg cgcgacgatg cagaagcacg ccggcgtgtt ccgcacgtcc gcgttgctca aggaaggcgt cgagcagatg gcgggcctga aggagcgcgc tgccagcgtc cacctgaagg acaagtcgaa ggtgttcaac accgcgcgcg tcgaagcgct cgaactggcg aacctgatcg aggtcgcgcg cgcgacgatg gtgtcggcgg aagcgcgcaa ggaaagccgc ggcgcgcacg cgcacagcga ttacgagcac cgcgacgacg agaactggct gcgtcacacg ctgtggtaca gcgaaggcga tcgcctcgac tacaagcccg tccaaatgaa gccgctgacg gtcgagtccg tgccgccgaa ggcgcgtacg ttctaa
Details for ButhA.18004.a.A1.GE32974
HARVESTED ON: 7/12/2011
SEQUENCED ON: 7/28/2011
EXPECTED MW: 66kDa
OBSERVED MW: 70kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 45
PERCENT COVERAGE: 32
Validated AA Sequence
MXHXXXXXMG TLEAXTQGPG SMAAIXTSLX XRXFDVVIVG AGGSXMRASL QLXXAGLSVC VLSXVXTRSH TVAAXGGIXA SLGNMXXXXW XYHFYXXMXX TGSATXTXSS SCAXSAXTSX XXSSTSACXS TVTRTXXSXS XXXAATXYXX XPVXRXXAXX DRXGHXXXXX XXXQNVXXKX XSSSNGWXXX XSAXXXATCS A
Validated NT Sequence
tnntttgtnn nntttnnnna ngagatatac catggntcat cnccntnnnn atnntatggg taccctggaa gctcanaccc agggtcctgg ttcgatggct gcnatcnaaa cttcnctgnc gcnncgcang ttcgacgtgg tgatcgtcgg cgcgggcggn tcnnggatgc gcgcgtcgct gcaactgtnn ngcgcgggcc tctccgtatg cgtgctgtcn aangtgttna cccgttcgca cacggtcgcc gcgcanggng ggatcngcgc gtcnctcggc aacatgancn aanacnactg gnnntaccac ttctacnann nnatggntnn nactggntcg gcnaccnnga cgcnntcnag ttcatgtgcc ncgagcgccn naacgtcntg nnnnanctcg agcacttcgg catgccnntc naccgtaacg cgnacggnnn natctannag cnncnnntnn gcggccacnn antacngnna gnanccggtt cancgngngt gngcngnngn cgaccgcann ggncacnnnn tgntgnnnnn nntgtancan cagaacgtcn nnnngaaacn cngttcttcg tcnaatggat ggnnctnnnn nngatccgcn annnnnangg cnacntgctc ngcgtgacgg cnctcnnnat gnanacngnc ganntctaca tcctctaang nnnnaccacn ntnntcgcca cgngcnnngc gnnncgatct ncnnnncgtc gnnnnnnnnn ntcatcanca cnnnnnacnn nntcnntggn nncccgntcn ngnatnncgc tgnnngacat gnagttctgg nnnttnnnnc nnanngncgt cnnnnncncn ngnntgctga tcacnnnaag nntgcgcngg gaangcngca tnntgcncnn nnnnnnnggc nagcnnnnnn aancnnnact nnccgacnnt nnanggatct nnnnnnannn gatnnnntgg anctnnnngg annnngnnat nannnnnnnn nngnnnnnnn nnnnnnaann tgntnnnnnn nntnnncnnn ntnnnnnnnn nncntnnngn nnnnnannnn annnnnnnnc nnnnnnnnnn nnnnnnnnnn nnnnnnannn nnnnnnngnn nnnannnnnn nnnnnnccnn nnnnnnngnn nnnnnnct
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAAIKTSLP RRKFDVVIVG AGGSGMRASL QLSRAGLSVC VLSKVFPTRS HTVAAQGGIG ASLGNMSEDN WHYHFYDTIK GSDWLGDQDA IEFMCREAPN VVYELEHFGM PFDRNADGTI YQRPFGGHTA NYGEKPVQRA CAAADRTGHA LLHTLYQQNV AAKTQFFVEW MALDLIRDAD GDVLGVTALE METGDVYILE GKTTLFATGG AGRIFAASTN AFINTGDGLG MAARSGIALQ DMEFWQFHPT GVAGAGVLIT EGVRGEGGIL RNANGERFME RYAPTLKDLA PRDFVSRSMD QEIKEGRGVG PNKDHVLLDL SHIGAETIMK RLPSIREIAL KFANVDCIKE PIPVVPTIHY QMGGIPTNIH GQVVGTSRDH KEPINGFYAV GECSCVSVHG ANRLGTNSLL DLVVFGRAAG NHIVEHVKNQ KEHKPLPADA AEFSLSRLAK LEKSTSGEYT QDIANDIRAT MQKHAGVFRT SALLKEGVEQ MAGLKERAAS VHLKDKSKVF NTARVEALEL ANLIEVARAT MVSAEARKES RGAHAHSDYE HRDDENWLRH TLWYSEGDRL DYKPVQMKPL TVESVPPKAR TF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggctgcaatc aaaacttccc tgccgcgtcg caagttcgac gtggtgatcg tcggcgcggg cggctcgggg atgcgcgcgt cgctgcaact gtcgcgcgcg ggcctctccg tatgcgtgct gtcgaaggtg ttcccgaccc gttcgcacac ggtcgccgcg caaggcggga tcggcgcgtc gctcggcaac atgagcgaag acaactggca ctaccacttc tacgacacga tcaagggctc cgactggctc ggcgaccagg acgcgatcga gttcatgtgc cgcgaagcgc cgaacgtcgt gtacgagctc gagcacttcg gcatgccgtt cgaccgtaac gcggacggca cgatctacca gcgtccgttc ggcggccaca cggccaacta cggcgagaag ccggttcagc gcgcgtgcgc ggcggccgac cgcaccggcc acgcgctgct gcacacgctg taccagcaga acgtcgcggc gaagacgcag ttcttcgtcg aatggatggc gctcgacctg atccgcgacg cggacggcga cgtgctcggc gtgacggccc tcgaaatgga aacgggcgac gtctacatcc tcgaaggcaa gaccacgctg ttcgccacgg gcggcgcggg ccggatcttc gcggcgtcga ccaacgcgtt catcaacacc ggcgacggcc tcggcatggc ggcccgctcg ggcatcgcgc tgcaggacat ggagttctgg caattccacc cgacgggcgt cgcgggcgcg ggcgtgctga tcaccgaagg cgtgcgcggc gaaggcggca ttctgcgcaa cgcgaacggc gagcgcttca tggagcgcta cgcgccgacg ctgaaggatc tggcgccgcg cgatttcgtg tcgcgctcga tggaccagga aatcaaggaa ggccgcggcg tgggcccgaa caaggatcac gtgctgctcg acctgtcgca catcggcgcg gagacgatca tgaagcgtct gccgtcgatc cgcgaaatcg cgctgaagtt cgcgaacgtc gactgcatca aggaaccgat tcccgtcgtg ccgacgatcc actaccagat gggcggcatc ccgaccaaca tccacggcca ggtggtgggc acgtcgcgcg atcacaagga gccgatcaac ggcttctacg cggtgggcga atgctcgtgc gtgtccgtgc acggcgcgaa ccgtctcggc acgaactcgc tgctcgacct cgtcgtgttc ggccgcgcgg ccggcaacca catcgtcgag cacgtgaaga accagaagga gcacaagccg ctgccggccg acgccgccga attctcgctg tcgcgcctcg cgaagctcga gaagtcgacg tcgggcgagt acacgcagga catcgcgaac gacatccgcg cgacgatgca gaagcacgcc ggcgtgttcc gcacgtccgc gttgctcaag gaaggcgtcg agcagatggc gggcctgaag gagcgcgctg ccagcgtcca cctgaaggac aagtcgaagg tgttcaacac cgcgcgcgtc gaagcgctcg aactggcgaa cctgatcgag gtcgcgcgcg cgacgatggt gtcggcggaa gcgcgcaagg aaagccgcgg cgcgcacgcg cacagcgatt acgagcaccg cgacgacgag aactggctgc gtcacacgct gtggtacagc gaaggcgatc gcctcgacta caagcccgtc caaatgaagc cgctgacggt cgagtccgtg ccgccgaagg cgcgtacgtt ctaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for ButhA.18004.a.A1.PS01241
PURIFICATION DATe: 10/19/2011
CONCENTRATION: 11.1mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 10
VIAL VOLUME: 200µl
PERCENT IDENTITY: 45
PERCENT COVERAGE: 32
Protocol Notes
notes unavailable
Validated AA Sequence
MXHXXXXXMG TLEAXTQGPG SMAAIXTSLX XRXFDVVIVG AGGSXMRASL QLXXAGLSVC VLSXVXTRSH TVAAXGGIXA SLGNMXXXXW XYHFYXXMXX TGSATXTXSS SCAXSAXTSX XXSSTSACXS TVTRTXXSXS XXXAATXYXX XPVXRXXAXX DRXGHXXXXX XXXQNVXXKX XSSSNGWXXX XSAXXXATCS A
Validated NT Sequence
tnntttgtnn nntttnnnna ngagatatac catggntcat cnccntnnnn atnntatggg taccctggaa gctcanaccc agggtcctgg ttcgatggct gcnatcnaaa cttcnctgnc gcnncgcang ttcgacgtgg tgatcgtcgg cgcgggcggn tcnnggatgc gcgcgtcgct gcaactgtnn ngcgcgggcc tctccgtatg cgtgctgtcn aangtgttna cccgttcgca cacggtcgcc gcgcanggng ggatcngcgc gtcnctcggc aacatgancn aanacnactg gnnntaccac ttctacnann nnatggntnn nactggntcg gcnaccnnga cgcnntcnag ttcatgtgcc ncgagcgccn naacgtcntg nnnnanctcg agcacttcgg catgccnntc naccgtaacg cgnacggnnn natctannag cnncnnntnn gcggccacnn antacngnna gnanccggtt cancgngngt gngcngnngn cgaccgcann ggncacnnnn tgntgnnnnn nntgtancan cagaacgtcn nnnngaaacn cngttcttcg tcnaatggat ggnnctnnnn nngatccgcn annnnnangg cnacntgctc ngcgtgacgg cnctcnnnat gnanacngnc ganntctaca tcctctaang nnnnaccacn ntnntcgcca cgngcnnngc gnnncgatct ncnnnncgtc gnnnnnnnnn ntcatcanca cnnnnnacnn nntcnntggn nncccgntcn ngnatnncgc tgnnngacat gnagttctgg nnnttnnnnc nnanngncgt cnnnnncncn ngnntgctga tcacnnnaag nntgcgcngg gaangcngca tnntgcncnn nnnnnnnggc nagcnnnnnn aancnnnact nnccgacnnt nnanggatct nnnnnnannn gatnnnntgg anctnnnngg annnngnnat nannnnnnnn nngnnnnnnn nnnnnnaann tgntnnnnnn nntnnncnnn ntnnnnnnnn nncntnnngn nnnnnannnn annnnnnnnc nnnnnnnnnn nnnnnnnnnn nnnnnnannn nnnnnnngnn nnnannnnnn nnnnnnccnn nnnnnnngnn nnnnnnct
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAAIKTSLP RRKFDVVIVG AGGSGMRASL QLSRAGLSVC VLSKVFPTRS HTVAAQGGIG ASLGNMSEDN WHYHFYDTIK GSDWLGDQDA IEFMCREAPN VVYELEHFGM PFDRNADGTI YQRPFGGHTA NYGEKPVQRA CAAADRTGHA LLHTLYQQNV AAKTQFFVEW MALDLIRDAD GDVLGVTALE METGDVYILE GKTTLFATGG AGRIFAASTN AFINTGDGLG MAARSGIALQ DMEFWQFHPT GVAGAGVLIT EGVRGEGGIL RNANGERFME RYAPTLKDLA PRDFVSRSMD QEIKEGRGVG PNKDHVLLDL SHIGAETIMK RLPSIREIAL KFANVDCIKE PIPVVPTIHY QMGGIPTNIH GQVVGTSRDH KEPINGFYAV GECSCVSVHG ANRLGTNSLL DLVVFGRAAG NHIVEHVKNQ KEHKPLPADA AEFSLSRLAK LEKSTSGEYT QDIANDIRAT MQKHAGVFRT SALLKEGVEQ MAGLKERAAS VHLKDKSKVF NTARVEALEL ANLIEVARAT MVSAEARKES RGAHAHSDYE HRDDENWLRH TLWYSEGDRL DYKPVQMKPL TVESVPPKAR TF
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCTGCAATC AAAACTTCCC TGCCGCGTCG CAAGTTCGAC GTGGTGATCG TCGGCGCGGG CGGCTCGGGG ATGCGCGCGT CGCTGCAACT GTCGCGCGCG GGCCTCTCCG TATGCGTGCT GTCGAAGGTG TTCCCGACCC GTTCGCACAC GGTCGCCGCG CAAGGCGGGA TCGGCGCGTC GCTCGGCAAC ATGAGCGAAG ACAACTGGCA CTACCACTTC TACGACACGA TCAAGGGCTC CGACTGGCTC GGCGACCAGG ACGCGATCGA GTTCATGTGC CGCGAAGCGC CGAACGTCGT GTACGAGCTC GAGCACTTCG GCATGCCGTT CGACCGTAAC GCGGACGGCA CGATCTACCA GCGTCCGTTC GGCGGCCACA CGGCCAACTA CGGCGAGAAG CCGGTTCAGC GCGCGTGCGC GGCGGCCGAC CGCACCGGCC ACGCGCTGCT GCACACGCTG TACCAGCAGA ACGTCGCGGC GAAGACGCAG TTCTTCGTCG AATGGATGGC GCTCGACCTG ATCCGCGACG CGGACGGCGA CGTGCTCGGC GTGACGGCCC TCGAAATGGA AACGGGCGAC GTCTACATCC TCGAAGGCAA GACCACGCTG TTCGCCACGG GCGGCGCGGG CCGGATCTTC GCGGCGTCGA CCAACGCGTT CATCAACACC GGCGACGGCC TCGGCATGGC GGCCCGCTCG GGCATCGCGC TGCAGGACAT GGAGTTCTGG CAATTCCACC CGACGGGCGT CGCGGGCGCG GGCGTGCTGA TCACCGAAGG CGTGCGCGGC GAAGGCGGCA TTCTGCGCAA CGCGAACGGC GAGCGCTTCA TGGAGCGCTA CGCGCCGACG CTGAAGGATC TGGCGCCGCG CGATTTCGTG TCGCGCTCGA TGGACCAGGA AATCAAGGAA GGCCGCGGCG TGGGCCCGAA CAAGGATCAC GTGCTGCTCG ACCTGTCGCA CATCGGCGCG GAGACGATCA TGAAGCGTCT GCCGTCGATC CGCGAAATCG CGCTGAAGTT CGCGAACGTC GACTGCATCA AGGAACCGAT TCCCGTCGTG CCGACGATCC ACTACCAGAT GGGCGGCATC CCGACCAACA TCCACGGCCA GGTGGTGGGC ACGTCGCGCG ATCACAAGGA GCCGATCAAC GGCTTCTACG CGGTGGGCGA ATGCTCGTGC GTGTCCGTGC ACGGCGCGAA CCGTCTCGGC ACGAACTCGC TGCTCGACCT CGTCGTGTTC GGCCGCGCGG CCGGCAACCA CATCGTCGAG CACGTGAAGA ACCAGAAGGA GCACAAGCCG CTGCCGGCCG ACGCCGCCGA ATTCTCGCTG TCGCGCCTCG CGAAGCTCGA GAAGTCGACG TCGGGCGAGT ACACGCAGGA CATCGCGAAC GACATCCGCG CGACGATGCA GAAGCACGCC GGCGTGTTCC GCACGTCCGC GTTGCTCAAG GAAGGCGTCG AGCAGATGGC GGGCCTGAAG GAGCGCGCTG CCAGCGTCCA CCTGAAGGAC AAGTCGAAGG TGTTCAACAC CGCGCGCGTC GAAGCGCTCG AACTGGCGAA CCTGATCGAG GTCGCGCGCG CGACGATGGT GTCGGCGGAA GCGCGCAAGG AAAGCCGCGG CGCGCACGCG CACAGCGATT ACGAGCACCG CGACGACGAG AACTGGCTGC GTCACACGCT GTGGTACAGC GAAGGCGATC GCCTCGACTA CAAGCCCGTC CAAATGAAGC CGCTGACGGT CGAGTCCGTG CCGCCGAAGG CGCGTACGTT CTAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA