SSGCID: Target Page

ButhA.17999.a

Adenylosuccinate lyase (EC 4.3.2.2) (BTH_I1221) (purB)

CENTER ID:	ButhA.17999.a
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.17999.a.A1.GE32878	full length		1	483

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.17999.a.A1.PW33482	full length		1	483

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.3979
RefSeq:	YP_441768.1
UniProt:	Q2SZ81

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MVKFAVLPFT FEPRPRASSP IMSDTRPDTL FALTALSPID GRYASKTEAL RDWLSEAAFM RHRVTVEVHW LIALSRAGFA EVPRFSEAAE QFLLQLVERF TAHDAARIKE IERVTNHDVK AVEYWLKESV KGQTELERAS EFIHFACTSE DINNTSHGMM LAGARQHVIL PALRTVHGRL VALAHAHAGQ PMLSRTHGQP ASPTTLGKEI ANVAARLARA IERIEKVEIL GKMNGAVGNF NAHLSAYPEF DWEAFSRDVI ENRLKLAFNP YTIQIEPHDY MAELFDAVAR ANTILLDLDR DVWGYISVGY FKQKTKAGEI GSSTMPHKVN PIDFENSEGN LGLANATLRH LADKLPVSRW QRDLTDSTVL RNMGVALGYS LLAYDSLIRG LDKLEVNPQR LNEDLDNCWE VLAEPVQTVM RRYGIENPYE QLKELTRGKG ITREALQQFI GTLAIPEDAK ARLLAMTPAS YIGKAAELAK RIA

NT Sequence

atggtaaaat tcgcggttct ccccttcacg ttcgaaccgc ggccgcgagc ctcaagcccg atcatgtccg acacccgtcc cgataccctt ttcgccctca ccgcgctttc gccgatcgac ggccgctacg ccagcaagac cgaagcgctg cgcgactggc tctccgaagc cgccttcatg cgccaccgcg tcaccgtcga ggtgcactgg ctgatcgcgc tgtcgcgcgc cggcttcgcc gaagtgccgc gcttctccga agccgccgaa cagttcctgc tgcagctcgt cgagcgcttc accgcgcacg acgccgcacg catcaaggaa atcgagcgcg tgacgaacca cgacgtgaaa gccgtcgagt actggctgaa ggaatccgtc aagggccaga ccgaactcga gcgcgcgagc gaattcatcc acttcgcgtg cacgtcggaa gacatcaaca acacgtcgca cggaatgatg cttgccggcg cgcgccagca cgtgatcctg cccgcgctgc gcacggtgca cggccgcctc gtcgcgctcg cgcacgcgca cgccgggcaa ccgatgctct cgcgcacgca cggccaaccg gccagcccga cgacgctcgg caaggaaatc gcgaacgtcg cggcgcgcct tgcgcgcgcg atcgagcgca tcgagaaggt cgagatcctc ggcaagatga acggcgcggt cggcaacttc aacgctcacc tgtccgccta tccggaattc gactgggaag cgttctcgcg cgacgtgatc gagaaccgcc tgaagctcgc gttcaacccg tacacgatcc agatcgaacc gcacgactac atggccgagc tgttcgacgc cgtggcgcgc gcgaacacga tcctgctcga cctcgaccgc gacgtgtggg gctacatctc ggtcggctac ttcaagcaga agacgaaggc gggcgaaatc ggctcgtcga cgatgccgca caaggtcaac ccgatcgact tcgaaaactc ggaaggcaac ctcggcctcg cgaacgcgac gctgcgccac ctcgccgaca agctgccggt gtcgcgctgg cagcgcgatc tcaccgattc gacggtgctg cgcaacatgg gcgtcgcgct cggctactcg ctgctcgcgt acgactcgct gatccgcggc ctcgacaagc tcgaggtgaa cccgcagcgc ctgaacgaag atctcgacaa ctgctgggaa gtgctcgccg agccggtgca gacggtgatg cgccgctacg gcatcgagaa cccgtacgag cagttgaagg aactgacgcg cggcaagggc atcacgcgcg aggcgctgca gcagttcatc ggcacgctcg cgattcccga ggacgcgaag gcgcgcctgc tcgcgatgac gcccgcgtcg tacatcggca aggcggccga gctcgcgaaa cgcatcgcgt aa

Details for ButhA.17999.a.A1.GE32878

HARVESTED ON:	6/28/2011
SEQUENCED ON:	7/22/2011
EXPECTED MW:	56kDa
OBSERVED MW:	56kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	32
PERCENT COVERAGE:	68

Validated AA Sequence

MXKFXVLPFT FEPRPXASSP XXSDTXXXTL FXLTXXSPID GRXXXKXEXL RDWXSEXXFX RHRXTVXVHW LIALSRAGFP XXRASPKPPN SSCCSCXALX RARRXXXXGN RAVTNXXXXA VEXWLKESQG PDRXXXXXRI HXXXVXVXXX XXXXXRNDAX XRAPAXXXAX AAXXAXSXRS RXXTXGXXCS XAXGXXXSXT TLXXEIXNXX ARLARAXXXI XXVXXLXXMX GXXXXLXRSX XXXXXEXDXX AFSRXXIXXR LXXXXXPXXX XIXXXXWXXX XXXXXXXXXX XXXXXXXXXX XXHXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXX

Validated NT Sequence

cnnnnnnnnn nnnntnnnna ttttgtttac tttaagaagg agatatacct ggntcatnnc cntcgnnntn gtnngggtac cctgnnnnct canacccagg gtcctggttn natggnnaaa ttcnnggttc tccccttcac gttcgaaccg cgnccgcnag cctcaagccc natnntgtcc gacacccntn nnnataccct tttcnncctc accnntnttt cgccgatcga cggccgctnn nccngcaaga ncgaancgct gcgcgactgg ntctccgaag ncnccttcnt gcgccaccgc ntcaccgtcn aggtgcactg gctgatcgcg ctgtcncgcg ccggcttccc nannngccgc gcttctccga agccgccgaa cagttcctgc tgcagctgtc nagcgcttcn ncgngcacga cgccncnnnn atcanggaaa tcgagcngtg acgaaccann acnngnaagc cgtcgagnnn tggctgaagg aatctcaagg gccagaccga nntcnnnngc nnnagcgaat tcatnnnntt cnngtgnnng tcnnangacn tcnncnacnc ntcncacgga atgatgctng nnggcgcgcg ccagcnnntg ntcntgccng cgctgcnnnn nnngcanngt cnncncgctc gcgcnnncnc acnncnggca annnatgctc tcnngcannn ggtcnancgg nnagccngac gacnctnnnn naggaaatcg ngaacntcnc ggcgcgnctt gcgcgcgcga tnnannncat cnannnngtc nagatnctcn nnaanatgaa nggnngngnn nngcnacttc nncgctcann ctgnnngnnc tannnngaan tcgacnggnn agcgttctcg cgcnannnga tcnannaccg tctgaanntc nnnntcaanc cgtnnncnnn ccnnatcgnn ncgantncnt ggnnnnannn ngntcnacnn nnnngnnnnn gnnncgannn natnntgnnn nncnnnnncn nnnnnnnnnn nnngnnncat nnnnnncngn tnnttnannn nanannannn nnnnnnnnnn nnntnnnnna nnnatnnnnn nnnnnnnnnn antnnnnnnn nntnnannnn nnnnnnnnnn cnnnnnnnnn nnnnnnnnnn nnnnannnnn nnng

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVKFAVLPF TFEPRPRASS PIMSDTRPDT LFALTALSPI DGRYASKTEA LRDWLSEAAF MRHRVTVEVH WLIALSRAGF AEVPRFSEAA EQFLLQLVER FTAHDAARIK EIERVTNHDV KAVEYWLKES VKGQTELERA SEFIHFACTS EDINNTSHGM MLAGARQHVI LPALRTVHGR LVALAHAHAG QPMLSRTHGQ PASPTTLGKE IANVAARLAR AIERIEKVEI LGKMNGAVGN FNAHLSAYPE FDWEAFSRDV IENRLKLAFN PYTIQIEPHD YMAELFDAVA RANTILLDLD RDVWGYISVG YFKQKTKAGE IGSSTMPHKV NPIDFENSEG NLGLANATLR HLADKLPVSR WQRDLTDSTV LRNMGVALGY SLLAYDSLIR GLDKLEVNPQ RLNEDLDNCW EVLAEPVQTV MRRYGIENPY EQLKELTRGK GITREALQQF IGTLAIPEDA KARLLAMTPA SYIGKAAELA KRIA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtaaaattc gcggttctcc ccttcacgtt cgaaccgcgg ccgcgagcct caagcccgat catgtccgac acccgtcccg ataccctttt cgccctcacc gcgctttcgc cgatcgacgg ccgctacgcc agcaagaccg aagcgctgcg cgactggctc tccgaagccg ccttcatgcg ccaccgcgtc accgtcgagg tgcactggct gatcgcgctg tcgcgcgccg gcttcgccga agtgccgcgc ttctccgaag ccgccgaaca gttcctgctg cagctcgtcg agcgcttcac cgcgcacgac gccgcacgca tcaaggaaat cgagcgcgtg acgaaccacg acgtgaaagc cgtcgagtac tggctgaagg aatccgtcaa gggccagacc gaactcgagc gcgcgagcga attcatccac ttcgcgtgca cgtcggaaga catcaacaac acgtcgcacg gaatgatgct tgccggcgcg cgccagcacg tgatcctgcc cgcgctgcgc acggtgcacg gccgcctcgt cgcgctcgcg cacgcgcacg ccgggcaacc gatgctctcg cgcacgcacg gccaaccggc cagcccgacg acgctcggca aggaaatcgc gaacgtcgcg gcgcgccttg cgcgcgcgat cgagcgcatc gagaaggtcg agatcctcgg caagatgaac ggcgcggtcg gcaacttcaa cgctcacctg tccgcctatc cggaattcga ctgggaagcg ttctcgcgcg acgtgatcga gaaccgcctg aagctcgcgt tcaacccgta cacgatccag atcgaaccgc acgactacat ggccgagctg ttcgacgccg tggcgcgcgc gaacacgatc ctgctcgacc tcgaccgcga cgtgtggggc tacatctcgg tcggctactt caagcagaag acgaaggcgg gcgaaatcgg ctcgtcgacg atgccgcaca aggtcaaccc gatcgacttc gaaaactcgg aaggcaacct cggcctcgcg aacgcgacgc tgcgccacct cgccgacaag ctgccggtgt cgcgctggca gcgcgatctc accgattcga cggtgctgcg caacatgggc gtcgcgctcg gctactcgct gctcgcgtac gactcgctga tccgcggcct cgacaagctc gaggtgaacc cgcagcgcct gaacgaagat ctcgacaact gctgggaagt gctcgccgag ccggtgcaga cggtgatgcg ccgctacggc atcgagaacc cgtacgagca gttgaaggaa ctgacgcgcg gcaagggcat cacgcgcgag gcgctgcagc agttcatcgg cacgctcgcg attcccgagg acgcgaaggc gcgcctgctc gcgatgacgc ccgcgtcgta catcggcaag gcggccgagc tcgcgaaacg catcgcgtaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa

Details for ButhA.17999.a.A1.PW33482

PURIFICATION DATe:	8/26/2011
CONCENTRATION:	35.1mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	5
VIAL VOLUME:	100µl
PERCENT IDENTITY:	32
PERCENT COVERAGE:	68

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTAAAATTC GCGGTTCTCC CCTTCACGTT CGAACCGCGG CCGCGAGCCT CAAGCCCGAT CATGTCCGAC ACCCGTCCCG ATACCCTTTT CGCCCTCACC GCGCTTTCGC CGATCGACGG CCGCTACGCC AGCAAGACCG AAGCGCTGCG CGACTGGCTC TCCGAAGCCG CCTTCATGCG CCACCGCGTC ACCGTCGAGG TGCACTGGCT GATCGCGCTG TCGCGCGCCG GCTTCGCCGA AGTGCCGCGC TTCTCCGAAG CCGCCGAACA GTTCCTGCTG CAGCTCGTCG AGCGCTTCAC CGCGCACGAC GCCGCACGCA TCAAGGAAAT CGAGCGCGTG ACGAACCACG ACGTGAAAGC CGTCGAGTAC TGGCTGAAGG AATCCGTCAA GGGCCAGACC GAACTCGAGC GCGCGAGCGA ATTCATCCAC TTCGCGTGCA CGTCGGAAGA CATCAACAAC ACGTCGCACG GAATGATGCT TGCCGGCGCG CGCCAGCACG TGATCCTGCC CGCGCTGCGC ACGGTGCACG GCCGCCTCGT CGCGCTCGCG CACGCGCACG CCGGGCAACC GATGCTCTCG CGCACGCACG GCCAACCGGC CAGCCCGACG ACGCTCGGCA AGGAAATCGC GAACGTCGCG GCGCGCCTTG CGCGCGCGAT CGAGCGCATC GAGAAGGTCG AGATCCTCGG CAAGATGAAC GGCGCGGTCG GCAACTTCAA CGCTCACCTG TCCGCCTATC CGGAATTCGA CTGGGAAGCG TTCTCGCGCG ACGTGATCGA GAACCGCCTG AAGCTCGCGT TCAACCCGTA CACGATCCAG ATCGAACCGC ACGACTACAT GGCCGAGCTG TTCGACGCCG TGGCGCGCGC GAACACGATC CTGCTCGACC TCGACCGCGA CGTGTGGGGC TACATCTCGG TCGGCTACTT CAAGCAGAAG ACGAAGGCGG GCGAAATCGG CTCGTCGACG ATGCCGCACA AGGTCAACCC GATCGACTTC GAAAACTCGG AAGGCAACCT CGGCCTCGCG AACGCGACGC TGCGCCACCT CGCCGACAAG CTGCCGGTGT CGCGCTGGCA GCGCGATCTC ACCGATTCGA CGGTGCTGCG CAACATGGGC GTCGCGCTCG GCTACTCGCT GCTCGCGTAC GACTCGCTGA TCCGCGGCCT CGACAAGCTC GAGGTGAACC CGCAGCGCCT GAACGAAGAT CTCGACAACT GCTGGGAAGT GCTCGCCGAG CCGGTGCAGA CGGTGATGCG CCGCTACGGC ATCGAGAACC CGTACGAGCA GTTGAAGGAA CTGACGCGCG GCAAGGGCAT CACGCGCGAG GCGCTGCAGC AGTTCATCGG CACGCTCGCG ATTCCCGAGG ACGCGAAGGC GCGCCTGCTC GCGATGACGC CCGCGTCGTA CATCGGCAAG GCGGCCGAGC TCGCGAAACG CATCGCGTAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA