ButhA.17993.a

Glycyl-tRNA synthetase, alpha chain (BTH_I0585)

CENTER ID: ButhA.17993.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.17993.a.A1.GE32970 full length 1 390
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.17993.a.A1.PS01202 full length 1 390

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.3274
RefSeq: YP_441142.1
UniProt: Q2T107

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEACPTPLRP HPPADPVKLH VSAQTQAARR ASCRRGAHRP RRLPEAERPA PRRPLMLTFQ QIILTLQSYW DRQGCALLQP IDMEVGAGTS HVHTFLRAIG PEPWRAAYVQ PSRRPKDGRY GENPNRLQHY YQYQVVLKPA PENILDLYLG SLAALGFDLK QNDVRFVEDD WENPTLGAWG LGWEVWLNGM EVTQFTYFQQ VGGLDCKPVL GEITYGLERL AMYLQKVENV YDLIWTEWEE QGPNGPEIRR LTYGDVYHQN EVEQSTYNFE QANVELLFTF FNSYEAEAKR MIEAQLALPA YELVLKAGHT FNLLDARGAI SVTERAAYIG RIRALSRLVA QAYYDSREKL GFPMIGNPVP GVPGLTTDAQ EAAQPAWAPP LKAERKIDQD
NT Sequence
atggaggcct gccccacccc gttgcgaccg cacccgcccg ccgatccggt aaaattacac gtttcagcac agacccaagc cgcgcgccgc gcgagctgcc ggcgcggcgc tcaccgcccc cgccgtctgc ccgaagcgga gcgcccggcg ccacgaaggc ctctcatgct tacgtttcag caaatcatcc tgacgctcca gtcctattgg gacaggcagg gttgcgccct gctccagccg atcgacatgg aagtcggcgc cggcacgtcg cacgtgcaca cgttcctgcg cgcgatcggc cccgagccgt ggcgcgccgc gtacgtgcag ccgtcgcgcc gcccgaagga cggccgctac ggcgagaacc cgaaccgcct gcagcactac taccagtacc aggtggtgct caagcccgcg ccggagaaca tcctcgatct gtatctcggc tcgctggcag cgctcggctt cgacctgaag cagaacgacg tgcgcttcgt cgaggacgac tgggaaaacc cgacgctcgg cgcatggggc ctcggctggg aagtgtggct gaacggaatg gaagtcacgc agttcacgta cttccagcag gtgggcggcc tcgactgcaa gcccgtgctc ggcgagatca cctatggcct cgagcgcctc gcgatgtatc tgcagaaggt cgagaacgtc tacgacctga tctggaccga gtgggaggag cagggcccga acggccccga gatccgccgt ctgacgtacg gcgatgtcta ccaccagaac gaagtcgagc agtcgacgta caacttcgag caggcgaacg tcgagctgct cttcacgttc ttcaacagct acgaggccga ggcgaagcgg atgatcgaag cgcagctcgc gctgcccgca tacgaactcg tgctgaaggc cggccacacg ttcaacctgc tcgacgcgcg cggcgcgatc tccgtgaccg agcgcgccgc gtacatcggc cgcatccgcg cgctgtcgcg gctcgtcgcg caggcgtact acgattcgcg cgagaagctc ggcttcccga tgatcggcaa cccggtgccg ggcgtgccgg gcttgaccac cgacgcgcag gaggccgcgc agcccgcatg ggccccgccg ctgaaagccg aacgcaagat cgatcaggac tga
Details for ButhA.17993.a.A1.GE32970
HARVESTED ON: 7/12/2011
SEQUENCED ON: 7/28/2011
EXPECTED MW: 46kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 89
Validated AA Sequence
MEACPTPLRP HPPADPVKLH VSAQTQAARR ASCRRGAHRP RRLPEAERPA PRRPLMLTFQ QIILTLQSYW DRQGCALLQP IDMEVGAGTS HVHTFLRAIG PEPWRAAYVQ PSRRPKDGRY GENPNRLQHY YQYQVVLKPA PENILDLYLG SLAALGFDLK QNDVRFVEDD WENPTLGAWG LGWEVWLNGM EVTQFTYFQQ VGGLDCKPVL GEITYGLERL AMYLQKVENV YDLIWTEWEE QGPNGPEIRR LTYGDVYHQN EVEQSTYNFE QANVELLFTF FNSYEAEAKR MIEAQLALPA YELVLKAGHT FNLLDARGAI SVTERAAYIG RIRALSRLVA QAYYDSREKL GFPMIGNPVP GVPGLTTDA
Validated NT Sequence
tgcgcgtcgg tggtcaagcc cggcacgccc ggcaccgggt tgccgatcat cgggaagccg agcttctcgc gcgaatcgta gtacgcctgc gcgacgagcc gcgacagcgc gcggatgcgg ccgatgtacg cggcgcgctc ggtcacggag atcgcgccgc gcgcgtcgag caggttgaac gtgtggccgg ccttcagcac gagttcgtat gcgggcagcg cgagctgcgc ttcgatcatc cgcttcgcct cggcctcgta gctgttgaag aacgtgaaga gcagctcgac gttcgcctgc tcgaagttgt acgtcgactg ctcgacttcg ttctggtggt agacatcgcc gtacgtcaga cggcggatct cggggccgtt cgggccctgc tcctcccact cggtccagat caggtcgtag acgttctcga ccttctgcag atacatcgcg aggcgctcga ggccataggt gatctcgccg agcacgggct tgcagtcgag gccgcccacc tgctggaagt acgtgaactg cgtgacttcc attccgttca gccacacttc ccagccgagg ccccatgcgc cgagcgtcgg gttttcccag tcgtcctcga cgaagcgcac gtcgttctgc ttcaggtcga agccgagcgc tgccagcgag ccgagataca gatcgaggat gttctccggc gcgggcttga gcaccacctg gtactggtag tagtgctgca ggcggttcgg gttctcgccg tagcggccgt ccttcgggcg gcgcgacggc tgcacgtacg cggcgcgcca cggctcgggg ccgatcgcgc gcaggaacgt gtgcacgtgc gacgtgccgg cgccgacttc catgtcgatc ggctggagca gggcgcaacc ctgcctgtcc caataggact ggagcgtcag gatgatttgc tgaaacgtaa gcatgagagg ccttcgtggc gccgggcgct ccgcttcggg cagacggcgg gggcggtgag cgccgcgccg gcagctcgcg cggcgcgcgg cttgggtctg tgctgaaacg tgtaatttta ccggatcggc gggcgggtgc ggtcgcaacg gggtggggca ggcctccatc gaaccaggac cctgggtctg agcttccagg gta
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMEACPTPLR PHPPADPVKL HVSAQTQAAR RASCRRGAHR PRRLPEAERP APRRPLMLTF QQIILTLQSY WDRQGCALLQ PIDMEVGAGT SHVHTFLRAI GPEPWRAAYV QPSRRPKDGR YGENPNRLQH YYQYQVVLKP APENILDLYL GSLAALGFDL KQNDVRFVED DWENPTLGAW GLGWEVWLNG MEVTQFTYFQ QVGGLDCKPV LGEITYGLER LAMYLQKVEN VYDLIWTEWE EQGPNGPEIR RLTYGDVYHQ NEVEQSTYNF EQANVELLFT FFNSYEAEAK RMIEAQLALP AYELVLKAGH TFNLLDARGA ISVTERAAYI GRIRALSRLV AQAYYDSREK LGFPMIGNPV PGVPGLTTDA QEAAQPAWAP PLKAERKIDQ D
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggaggcctgc cccaccccgt tgcgaccgca cccgcccgcc gatccggtaa aattacacgt ttcagcacag acccaagccg cgcgccgcgc gagctgccgg cgcggcgctc accgcccccg ccgtctgccc gaagcggagc gcccggcgcc acgaaggcct ctcatgctta cgtttcagca aatcatcctg acgctccagt cctattggga caggcagggt tgcgccctgc tccagccgat cgacatggaa gtcggcgccg gcacgtcgca cgtgcacacg ttcctgcgcg cgatcggccc cgagccgtgg cgcgccgcgt acgtgcagcc gtcgcgccgc ccgaaggacg gccgctacgg cgagaacccg aaccgcctgc agcactacta ccagtaccag gtggtgctca agcccgcgcc ggagaacatc ctcgatctgt atctcggctc gctggcagcg ctcggcttcg acctgaagca gaacgacgtg cgcttcgtcg aggacgactg ggaaaacccg acgctcggcg catggggcct cggctgggaa gtgtggctga acggaatgga agtcacgcag ttcacgtact tccagcaggt gggcggcctc gactgcaagc ccgtgctcgg cgagatcacc tatggcctcg agcgcctcgc gatgtatctg cagaaggtcg agaacgtcta cgacctgatc tggaccgagt gggaggagca gggcccgaac ggccccgaga tccgccgtct gacgtacggc gatgtctacc accagaacga agtcgagcag tcgacgtaca acttcgagca ggcgaacgtc gagctgctct tcacgttctt caacagctac gaggccgagg cgaagcggat gatcgaagcg cagctcgcgc tgcccgcata cgaactcgtg ctgaaggccg gccacacgtt caacctgctc gacgcgcgcg gcgcgatctc cgtgaccgag cgcgccgcgt acatcggccg catccgcgcg ctgtcgcggc tcgtcgcgca ggcgtactac gattcgcgcg agaagctcgg cttcccgatg atcggcaacc cggtgccggg cgtgccgggc ttgaccaccg acgcgcagga ggccgcgcag cccgcatggg ccccgccgct gaaagccgaa cgcaagatcg atcaggacta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for ButhA.17993.a.A1.PS01202
PURIFICATION DATe: 9/7/2011
CONCENTRATION: 2.41mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 89
Protocol Notes
notes unavailable
Validated AA Sequence
MEACPTPLRP HPPADPVKLH VSAQTQAARR ASCRRGAHRP RRLPEAERPA PRRPLMLTFQ QIILTLQSYW DRQGCALLQP IDMEVGAGTS HVHTFLRAIG PEPWRAAYVQ PSRRPKDGRY GENPNRLQHY YQYQVVLKPA PENILDLYLG SLAALGFDLK QNDVRFVEDD WENPTLGAWG LGWEVWLNGM EVTQFTYFQQ VGGLDCKPVL GEITYGLERL AMYLQKVENV YDLIWTEWEE QGPNGPEIRR LTYGDVYHQN EVEQSTYNFE QANVELLFTF FNSYEAEAKR MIEAQLALPA YELVLKAGHT FNLLDARGAI SVTERAAYIG RIRALSRLVA QAYYDSREKL GFPMIGNPVP GVPGLTTDA
Validated NT Sequence
tgcgcgtcgg tggtcaagcc cggcacgccc ggcaccgggt tgccgatcat cgggaagccg agcttctcgc gcgaatcgta gtacgcctgc gcgacgagcc gcgacagcgc gcggatgcgg ccgatgtacg cggcgcgctc ggtcacggag atcgcgccgc gcgcgtcgag caggttgaac gtgtggccgg ccttcagcac gagttcgtat gcgggcagcg cgagctgcgc ttcgatcatc cgcttcgcct cggcctcgta gctgttgaag aacgtgaaga gcagctcgac gttcgcctgc tcgaagttgt acgtcgactg ctcgacttcg ttctggtggt agacatcgcc gtacgtcaga cggcggatct cggggccgtt cgggccctgc tcctcccact cggtccagat caggtcgtag acgttctcga ccttctgcag atacatcgcg aggcgctcga ggccataggt gatctcgccg agcacgggct tgcagtcgag gccgcccacc tgctggaagt acgtgaactg cgtgacttcc attccgttca gccacacttc ccagccgagg ccccatgcgc cgagcgtcgg gttttcccag tcgtcctcga cgaagcgcac gtcgttctgc ttcaggtcga agccgagcgc tgccagcgag ccgagataca gatcgaggat gttctccggc gcgggcttga gcaccacctg gtactggtag tagtgctgca ggcggttcgg gttctcgccg tagcggccgt ccttcgggcg gcgcgacggc tgcacgtacg cggcgcgcca cggctcgggg ccgatcgcgc gcaggaacgt gtgcacgtgc gacgtgccgg cgccgacttc catgtcgatc ggctggagca gggcgcaacc ctgcctgtcc caataggact ggagcgtcag gatgatttgc tgaaacgtaa gcatgagagg ccttcgtggc gccgggcgct ccgcttcggg cagacggcgg gggcggtgag cgccgcgccg gcagctcgcg cggcgcgcgg cttgggtctg tgctgaaacg tgtaatttta ccggatcggc gggcgggtgc ggtcgcaacg gggtggggca ggcctccatc gaaccaggac cctgggtctg agcttccagg gta
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMEACPTPLR PHPPADPVKL HVSAQTQAAR RASCRRGAHR PRRLPEAERP APRRPLMLTF QQIILTLQSY WDRQGCALLQ PIDMEVGAGT SHVHTFLRAI GPEPWRAAYV QPSRRPKDGR YGENPNRLQH YYQYQVVLKP APENILDLYL GSLAALGFDL KQNDVRFVED DWENPTLGAW GLGWEVWLNG MEVTQFTYFQ QVGGLDCKPV LGEITYGLER LAMYLQKVEN VYDLIWTEWE EQGPNGPEIR RLTYGDVYHQ NEVEQSTYNF EQANVELLFT FFNSYEAEAK RMIEAQLALP AYELVLKAGH TFNLLDARGA ISVTERAAYI GRIRALSRLV AQAYYDSREK LGFPMIGNPV PGVPGLTTDA QEAAQPAWAP PLKAERKIDQ D
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGAGGCCTGC CCCACCCCGT TGCGACCGCA CCCGCCCGCC GATCCGGTAA AATTACACGT TTCAGCACAG ACCCAAGCCG CGCGCCGCGC GAGCTGCCGG CGCGGCGCTC ACCGCCCCCG CCGTCTGCCC GAAGCGGAGC GCCCGGCGCC ACGAAGGCCT CTCATGCTTA CGTTTCAGCA AATCATCCTG ACGCTCCAGT CCTATTGGGA CAGGCAGGGT TGCGCCCTGC TCCAGCCGAT CGACATGGAA GTCGGCGCCG GCACGTCGCA CGTGCACACG TTCCTGCGCG CGATCGGCCC CGAGCCGTGG CGCGCCGCGT ACGTGCAGCC GTCGCGCCGC CCGAAGGACG GCCGCTACGG CGAGAACCCG AACCGCCTGC AGCACTACTA CCAGTACCAG GTGGTGCTCA AGCCCGCGCC GGAGAACATC CTCGATCTGT ATCTCGGCTC GCTGGCAGCG CTCGGCTTCG ACCTGAAGCA GAACGACGTG CGCTTCGTCG AGGACGACTG GGAAAACCCG ACGCTCGGCG CATGGGGCCT CGGCTGGGAA GTGTGGCTGA ACGGAATGGA AGTCACGCAG TTCACGTACT TCCAGCAGGT GGGCGGCCTC GACTGCAAGC CCGTGCTCGG CGAGATCACC TATGGCCTCG AGCGCCTCGC GATGTATCTG CAGAAGGTCG AGAACGTCTA CGACCTGATC TGGACCGAGT GGGAGGAGCA GGGCCCGAAC GGCCCCGAGA TCCGCCGTCT GACGTACGGC GATGTCTACC ACCAGAACGA AGTCGAGCAG TCGACGTACA ACTTCGAGCA GGCGAACGTC GAGCTGCTCT TCACGTTCTT CAACAGCTAC GAGGCCGAGG CGAAGCGGAT GATCGAAGCG CAGCTCGCGC TGCCCGCATA CGAACTCGTG CTGAAGGCCG GCCACACGTT CAACCTGCTC GACGCGCGCG GCGCGATCTC CGTGACCGAG CGCGCCGCGT ACATCGGCCG CATCCGCGCG CTGTCGCGGC TCGTCGCGCA GGCGTACTAC GATTCGCGCG AGAAGCTCGG CTTCCCGATG ATCGGCAACC CGGTGCCGGG CGTGCCGGGC TTGACCACCG ACGCGCAGGA GGCCGCGCAG CCCGCATGGG CCCCGCCGCT GAAAGCCGAA CGCAAGATCG ATCAGGACTA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A