ButhA.17991.a
Glutamate dehydrogenase (BTH_I1224)
| CENTER ID: | ButhA.17991.a |
| ORGANISM: | Burkholderia thailandensis E264 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.17991.a.A1.GE32871 | full length | 1 | 434 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.17991.a.A1.PW33475 | full length | 1 | 434 |
Structures
4XGI
DEPOSITED: 12/30/2014
DETERMINATION: XRay
CLONE: ButhA.17991.a.A1.GE32871
PROTEIN: ButhA.17991.a.A1.PW33475
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_126857 |
| PATRIC ID: | fig|271848.6.peg.3983 |
| RefSeq: | YP_441771.1 |
| UniProt: | Q2SZ78 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSQSQSPSV AQSIPSYLHA DDLGPWGNYL QQVDRVAPYL GSLSRWIETL KRPKRILIVD VPIELDNGTV AHFEGYRVQH NVSRGPGKGG VRYHQDVTLS EVMALSAWMS VKNAAVNVPY GGAKGGIRVD PRKLSRGELE RVTRRYTSEI GIIIGPNTDI PAPDVNTNEQ IMAWMMDTYS MNQGQTATGV VTGKPISLGG SLGRKEATGR GVFVVGCEAA KKKGVEIEGA RIAVQGFGNV GGIAAKLFQE AGAKVIAVQD HTGTIHQPAG VDTAKLLDHV GRTGGVAGFE GAEPMPNDEF WTVETEILIP AALENQITEK NASKIRTKII VEGANGPTTT AADDILSANG VLVIPDVIAN AGGVTVSYFE WVQDFSSFFW TEDEINHRLE RVMREAFAGV WAVAEEHKVS VRTAAFIVAC KRILMAREMR GLYP
NT Sequence
atgtcttcgc aatcgcagtc cccgtccgtc gcgcagtcca ttccgtcgta cctgcacgcg gacgatctcg gcccctgggg caactacctg cagcaagtcg atcgcgtcgc gccgtacctc ggctccctgt cgcgctggat cgagacgctg aagcgcccga agcgcatcct gatcgtcgat gtgccgatcg agctggacaa cggcaccgtt gcgcatttcg agggctatcg cgtgcagcac aacgtgtcgc gcggcccggg caagggcggc gtgcgttacc accaggacgt gacgctgtcc gaggtgatgg cgctgtccgc ctggatgtcg gtgaagaacg cggccgtgaa cgtgccgtac ggcggcgcga agggcggcat ccgcgtcgat ccgcgcaagc tctcacgtgg cgagctcgag cgcgtaacgc gtcgctacac cagcgaaatc ggcatcatca tcggcccgaa caccgatatt cccgcgcctg acgtcaacac gaacgagcag atcatggcgt ggatgatgga cacttactcg atgaaccagg gccagacggc cacgggcgtc gtgaccggca agccgatctc gctcggcggc tcgctcggcc gcaaggaagc gactggccgc ggcgtgttcg tcgtcggctg cgaggcggcg aagaagaagg gtgtcgagat cgaaggcgcg cgcatcgcgg tgcagggttt cggcaacgtc ggcgggatcg ccgcgaagct gttccaggaa gcgggcgcga aggtgatcgc ggtgcaggat cacacgggca cgatccacca gccggctggc gtcgacacgg ccaagctgct cgaccacgtc ggccgcacgg gcggcgtcgc gggcttcgaa ggcgcggaac cgatgccgaa cgacgagttc tggaccgtcg agaccgagat cctgattccg gccgcgctgg aaaaccagat caccgagaag aacgcgtcga agatccgcac gaagatcatc gtcgaaggcg cgaacggccc gacgacgacg gccgcggacg acatcctgag cgcgaacggc gtgctcgtga tccccgacgt gatcgcgaac gcgggcggcg tgaccgtgtc gtacttcgag tgggtgcagg atttctcgag cttcttctgg accgaagacg agatcaacca ccgtctcgag cgcgtgatgc gcgaggcgtt tgccggcgtg tgggcggtgg ccgaagagca caaggtgtcg gtgcgcacgg cggcgttcat cgtcgcgtgc aaacgcatcc tgatggcgcg cgaaatgcgc ggtctgtacc cctga
Details for ButhA.17991.a.A1.GE32871
| HARVESTED ON: | 6/28/2011 |
| SEQUENCED ON: | 7/22/2011 |
| EXPECTED MW: | 49kDa |
| OBSERVED MW: | 49kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 94 |
Validated AA Sequence
MSSQSQSPSV AQSIPSYLHA DDLGPWGNYL QQVDRVAPYL GSLSRWIETL KRPKRILIVD VPIELDNGTV AHFEGYRVQH NVSRGPGKGG VRYHQDVTLS EVMALSAWMS VKNAAVNVPY GGAKGGIRVD PRKLSRGELE RVTRRYTSEI GIIIGPNTDI PAPDVNTNEQ IMAWMMDTYS MNQGQTATGV VTGKPISLGG SLGRKEATGR GVFVVGCEAA KKKGVEIEGA RIAVQGFGNV GGIAAKLFQE AGAKVIAVQD HTGTIHQPAG VDTAKLLDHV GRTGGVAGFE GAEPMPNDEF WTVETEILIP AALENQITEK NASKIRTKII VEGANGPTTT AADDILSANG VLVIPDVIAN AGGVTVSYFE WVQDFSSFFW TEDEINHRLE RVMREAFAGV WAVAEEHKVS VRTAAFIVAC KRILMAREMR GL
Validated NT Sequence
acagaccgcg catttcgcgc gccatcagga tgcgtttgca cgcgacgatg aacgccgccg tgcgcaccga caccttgtgc tcttcggcca ccgcccacac gccggcaaac gcctcgcgca tcacgcgctc gagacggtgg ttgatctcgt cttcggtcca gaagaagctc gagaaatcct gcacccactc gaagtacgac acggtcacgc cgcccgcgtt cgcgatcacg tcggggatca cgagcacgcc gttcgcgctc aggatgtcgt ccgcggccgt cgtcgtcggg ccgttcgcgc cttcgacgat gatcttcgtg cggatcttcg acgcgttctt ctcggtgatc tggttttcca gcgcggccgg aatcaggatc tcggtctcga cggtccagaa ctcgtcgttc ggcatcggtt ccgcgccttc gaagcccgcg acgccgcccg tgcggccgac gtggtcgagc agcttggccg tgtcgacgcc agccggctgg tggatcgtgc ccgtgtgatc ctgcaccgcg atcaccttcg cgcccgcttc ctggaacagc ttcgcggcga tcccgccgac gttgccgaaa ccctgcaccg cgatgcgcgc gccttcgatc tcgacaccct tcttcttcgc cgcctcgcag ccgacgacga acacgccgcg gccagtcgct tccttgcggc cgagcgagcc gccgagcgag atcggcttgc cggtcacgac gcccgtggcc gtctggccct ggttcatcga gtaagtgtcc atcatccacg ccatgatctg ctcgttcgtg ttgacgtcag gcgcgggaat atcggtgttc gggccgatga tgatgccgat ttcgctggtg tagcgacgcg ttacgcgctc gagctcgcca cgtgagagct tgcgcggatc gacgcggatg ccgcccttcg cgccgccgta cggcacgttc acggccgcgt tcttcaccga catccaggcg gacagcgcca tcacctcgga cagcgtcacg tcctggtggt aacgcacgcc gcccttgccc gggccgcgcg acacgttgtg ctgcacgcga tagccctcga aatgcgcaac ggtgccgttg tccagctcga tcggcacatc gacgatcagg atgcgcttcg ggcgcttcag cgtctcgatc cagcgcgaca gggagccgag gtacggcgcg acgcgatcga cttgctgcag gtagttgccc caggggccga gatcgtccgc gtgcaggtac gacggaatgg actgcgcgac ggacggggac tgcgattgcg aagacatcga accaggaccc tgggtctgag ctt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSSQSQSPS VAQSIPSYLH ADDLGPWGNY LQQVDRVAPY LGSLSRWIET LKRPKRILIV DVPIELDNGT VAHFEGYRVQ HNVSRGPGKG GVRYHQDVTL SEVMALSAWM SVKNAAVNVP YGGAKGGIRV DPRKLSRGEL ERVTRRYTSE IGIIIGPNTD IPAPDVNTNE QIMAWMMDTY SMNQGQTATG VVTGKPISLG GSLGRKEATG RGVFVVGCEA AKKKGVEIEG ARIAVQGFGN VGGIAAKLFQ EAGAKVIAVQ DHTGTIHQPA GVDTAKLLDH VGRTGGVAGF EGAEPMPNDE FWTVETEILI PAALENQITE KNASKIRTKI IVEGANGPTT TAADDILSAN GVLVIPDVIA NAGGVTVSYF EWVQDFSSFF WTEDEINHRL ERVMREAFAG VWAVAEEHKV SVRTAAFIVA CKRILMAREM RGLYP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcttcgcaa tcgcagtccc cgtccgtcgc gcagtccatt ccgtcgtacc tgcacgcgga cgatctcggc ccctggggca actacctgca gcaagtcgat cgcgtcgcgc cgtacctcgg ctccctgtcg cgctggatcg agacgctgaa gcgcccgaag cgcatcctga tcgtcgatgt gccgatcgag ctggacaacg gcaccgttgc gcatttcgag ggctatcgcg tgcagcacaa cgtgtcgcgc ggcccgggca agggcggcgt gcgttaccac caggacgtga cgctgtccga ggtgatggcg ctgtccgcct ggatgtcggt gaagaacgcg gccgtgaacg tgccgtacgg cggcgcgaag ggcggcatcc gcgtcgatcc gcgcaagctc tcacgtggcg agctcgagcg cgtaacgcgt cgctacacca gcgaaatcgg catcatcatc ggcccgaaca ccgatattcc cgcgcctgac gtcaacacga acgagcagat catggcgtgg atgatggaca cttactcgat gaaccagggc cagacggcca cgggcgtcgt gaccggcaag ccgatctcgc tcggcggctc gctcggccgc aaggaagcga ctggccgcgg cgtgttcgtc gtcggctgcg aggcggcgaa gaagaagggt gtcgagatcg aaggcgcgcg catcgcggtg cagggtttcg gcaacgtcgg cgggatcgcc gcgaagctgt tccaggaagc gggcgcgaag gtgatcgcgg tgcaggatca cacgggcacg atccaccagc cggctggcgt cgacacggcc aagctgctcg accacgtcgg ccgcacgggc ggcgtcgcgg gcttcgaagg cgcggaaccg atgccgaacg acgagttctg gaccgtcgag accgagatcc tgattccggc cgcgctggaa aaccagatca ccgagaagaa cgcgtcgaag atccgcacga agatcatcgt cgaaggcgcg aacggcccga cgacgacggc cgcggacgac atcctgagcg cgaacggcgt gctcgtgatc cccgacgtga tcgcgaacgc gggcggcgtg accgtgtcgt acttcgagtg ggtgcaggat ttctcgagct tcttctggac cgaagacgag atcaaccacc gtctcgagcg cgtgatgcgc gaggcgtttg ccggcgtgtg ggcggtggcc gaagagcaca aggtgtcggt gcgcacggcg gcgttcatcg tcgcgtgcaa acgcatcctg atggcgcgcg aaatgcgcgg tctgtacccc taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for ButhA.17991.a.A1.PW33475
| PURIFICATION DATe: | 9/16/2011 |
| CONCENTRATION: | 41.55mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 1 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 94 |
Protocol Notes
notes unavailable
Validated AA Sequence
MSSQSQSPSV AQSIPSYLHA DDLGPWGNYL QQVDRVAPYL GSLSRWIETL KRPKRILIVD VPIELDNGTV AHFEGYRVQH NVSRGPGKGG VRYHQDVTLS EVMALSAWMS VKNAAVNVPY GGAKGGIRVD PRKLSRGELE RVTRRYTSEI GIIIGPNTDI PAPDVNTNEQ IMAWMMDTYS MNQGQTATGV VTGKPISLGG SLGRKEATGR GVFVVGCEAA KKKGVEIEGA RIAVQGFGNV GGIAAKLFQE AGAKVIAVQD HTGTIHQPAG VDTAKLLDHV GRTGGVAGFE GAEPMPNDEF WTVETEILIP AALENQITEK NASKIRTKII VEGANGPTTT AADDILSANG VLVIPDVIAN AGGVTVSYFE WVQDFSSFFW TEDEINHRLE RVMREAFAGV WAVAEEHKVS VRTAAFIVAC KRILMAREMR GL
Validated NT Sequence
acagaccgcg catttcgcgc gccatcagga tgcgtttgca cgcgacgatg aacgccgccg tgcgcaccga caccttgtgc tcttcggcca ccgcccacac gccggcaaac gcctcgcgca tcacgcgctc gagacggtgg ttgatctcgt cttcggtcca gaagaagctc gagaaatcct gcacccactc gaagtacgac acggtcacgc cgcccgcgtt cgcgatcacg tcggggatca cgagcacgcc gttcgcgctc aggatgtcgt ccgcggccgt cgtcgtcggg ccgttcgcgc cttcgacgat gatcttcgtg cggatcttcg acgcgttctt ctcggtgatc tggttttcca gcgcggccgg aatcaggatc tcggtctcga cggtccagaa ctcgtcgttc ggcatcggtt ccgcgccttc gaagcccgcg acgccgcccg tgcggccgac gtggtcgagc agcttggccg tgtcgacgcc agccggctgg tggatcgtgc ccgtgtgatc ctgcaccgcg atcaccttcg cgcccgcttc ctggaacagc ttcgcggcga tcccgccgac gttgccgaaa ccctgcaccg cgatgcgcgc gccttcgatc tcgacaccct tcttcttcgc cgcctcgcag ccgacgacga acacgccgcg gccagtcgct tccttgcggc cgagcgagcc gccgagcgag atcggcttgc cggtcacgac gcccgtggcc gtctggccct ggttcatcga gtaagtgtcc atcatccacg ccatgatctg ctcgttcgtg ttgacgtcag gcgcgggaat atcggtgttc gggccgatga tgatgccgat ttcgctggtg tagcgacgcg ttacgcgctc gagctcgcca cgtgagagct tgcgcggatc gacgcggatg ccgcccttcg cgccgccgta cggcacgttc acggccgcgt tcttcaccga catccaggcg gacagcgcca tcacctcgga cagcgtcacg tcctggtggt aacgcacgcc gcccttgccc gggccgcgcg acacgttgtg ctgcacgcga tagccctcga aatgcgcaac ggtgccgttg tccagctcga tcggcacatc gacgatcagg atgcgcttcg ggcgcttcag cgtctcgatc cagcgcgaca gggagccgag gtacggcgcg acgcgatcga cttgctgcag gtagttgccc caggggccga gatcgtccgc gtgcaggtac gacggaatgg actgcgcgac ggacggggac tgcgattgcg aagacatcga accaggaccc tgggtctgag ctt
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSSQSQSPS VAQSIPSYLH ADDLGPWGNY LQQVDRVAPY LGSLSRWIET LKRPKRILIV DVPIELDNGT VAHFEGYRVQ HNVSRGPGKG GVRYHQDVTL SEVMALSAWM SVKNAAVNVP YGGAKGGIRV DPRKLSRGEL ERVTRRYTSE IGIIIGPNTD IPAPDVNTNE QIMAWMMDTY SMNQGQTATG VVTGKPISLG GSLGRKEATG RGVFVVGCEA AKKKGVEIEG ARIAVQGFGN VGGIAAKLFQ EAGAKVIAVQ DHTGTIHQPA GVDTAKLLDH VGRTGGVAGF EGAEPMPNDE FWTVETEILI PAALENQITE KNASKIRTKI IVEGANGPTT TAADDILSAN GVLVIPDVIA NAGGVTVSYF EWVQDFSSFF WTEDEINHRL ERVMREAFAG VWAVAEEHKV SVRTAAFIVA CKRILMAREM RGLYP
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCTTCGCAA TCGCAGTCCC CGTCCGTCGC GCAGTCCATT CCGTCGTACC TGCACGCGGA CGATCTCGGC CCCTGGGGCA ACTACCTGCA GCAAGTCGAT CGCGTCGCGC CGTACCTCGG CTCCCTGTCG CGCTGGATCG AGACGCTGAA GCGCCCGAAG CGCATCCTGA TCGTCGATGT GCCGATCGAG CTGGACAACG GCACCGTTGC GCATTTCGAG GGCTATCGCG TGCAGCACAA CGTGTCGCGC GGCCCGGGCA AGGGCGGCGT GCGTTACCAC CAGGACGTGA CGCTGTCCGA GGTGATGGCG CTGTCCGCCT GGATGTCGGT GAAGAACGCG GCCGTGAACG TGCCGTACGG CGGCGCGAAG GGCGGCATCC GCGTCGATCC GCGCAAGCTC TCACGTGGCG AGCTCGAGCG CGTAACGCGT CGCTACACCA GCGAAATCGG CATCATCATC GGCCCGAACA CCGATATTCC CGCGCCTGAC GTCAACACGA ACGAGCAGAT CATGGCGTGG ATGATGGACA CTTACTCGAT GAACCAGGGC CAGACGGCCA CGGGCGTCGT GACCGGCAAG CCGATCTCGC TCGGCGGCTC GCTCGGCCGC AAGGAAGCGA CTGGCCGCGG CGTGTTCGTC GTCGGCTGCG AGGCGGCGAA GAAGAAGGGT GTCGAGATCG AAGGCGCGCG CATCGCGGTG CAGGGTTTCG GCAACGTCGG CGGGATCGCC GCGAAGCTGT TCCAGGAAGC GGGCGCGAAG GTGATCGCGG TGCAGGATCA CACGGGCACG ATCCACCAGC CGGCTGGCGT CGACACGGCC AAGCTGCTCG ACCACGTCGG CCGCACGGGC GGCGTCGCGG GCTTCGAAGG CGCGGAACCG ATGCCGAACG ACGAGTTCTG GACCGTCGAG ACCGAGATCC TGATTCCGGC CGCGCTGGAA AACCAGATCA CCGAGAAGAA CGCGTCGAAG ATCCGCACGA AGATCATCGT CGAAGGCGCG AACGGCCCGA CGACGACGGC CGCGGACGAC ATCCTGAGCG CGAACGGCGT GCTCGTGATC CCCGACGTGA TCGCGAACGC GGGCGGCGTG ACCGTGTCGT ACTTCGAGTG GGTGCAGGAT TTCTCGAGCT TCTTCTGGAC CGAAGACGAG ATCAACCACC GTCTCGAGCG CGTGATGCGC GAGGCGTTTG CCGGCGTGTG GGCGGTGGCC GAAGAGCACA AGGTGTCGGT GCGCACGGCG GCGTTCATCG TCGCGTGCAA ACGCATCCTG ATGGCGCGCG AAATGCGCGG TCTGTACCCC TAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA