ButhA.17985.a

N utilization substance protein A (BTH_I2565)

CENTER ID: ButhA.17985.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.17985.a.A1.GE32865 full length 1 491
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.17985.a.A1.PW33469 full length 1 491

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.5420
RefSeq: YP_443082.1
UniProt: Q2SVG7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSREVLMLVD ALAREKNVDK DVVLGALEAA LASASKKLFD EGAEIRVHID RESGEHETFR RWLVVPDEAG LQEPDREILL FEAREQKPGV EVGDYIEEPV PSIEFGRIGA QAAKQVILQK VRDAEREQIL NDYLERGEKI MTGTVKRLDK GNFIVESGRV EALLRRDQLI PKENLRVGDR VRAYIAKVDR TARGPQIELS RTAPEFLMKL FEMEVPEIEQ GLLEIKAAAR DPGVRAKIGV VAYDKRIDPI GTCVGIRGSR VQAVRNELGG ENIDIVLWSE DPAQFVIGAL APAAVQSIVV DEEKHSMDVV VDENELAVAI GRSGQNVRLA SELTGWQINI MTPDESAQKQ NEERDALRGL FMARLDVDEE VADILIDEGF TSLEEIAYVP LNEMLEIEAF DEDTVHELRN RSRDALLTMA IANEEKVETA ALDLKSLDGV TPELLAKLAE QSVQTRDDLA ELAVDELVDM TGMEEEAAKA LIMKAREHWF Q
NT Sequence
atgagtcgcg aagtgttgat gttggtggat gcgctggcgc gcgagaagaa cgtcgacaag gacgtcgtgc tgggcgcgct cgaagcggcc ctcgcgtcgg cttccaagaa gctgttcgac gaaggcgccg agatccgcgt acatatcgat cgcgagagcg gtgaacacga gacgttccgt cgttggctcg tcgtgcccga cgaggcgggc ctccaggagc cggatcgcga gatcctgctg ttcgaggcgc gcgagcagaa gcccggcgtc gaggtcggcg actacatcga ggagccggtg ccgtcgatcg agttcggccg gatcggcgcg caggccgcga agcaggtgat cctgcagaag gtgcgcgacg cggagcgcga gcagatcctg aacgactacc tcgagcgcgg cgagaaaatc atgacgggca cggtgaagcg cctcgacaag ggcaacttca tcgtcgaatc gggccgcgtc gaggcgctgt tgcgccgcga ccagctgatt ccgaaggaaa acctgcgcgt gggcgaccgc gtgcgcgcgt acatcgcgaa ggtcgaccgc accgctcgcg gcccgcagat cgagctgtcg cgcaccgcgc ccgaattcct gatgaagctc ttcgagatgg aagtgccgga aatcgagcag gggcttctcg agatcaaggc ggcggctcgc gatccgggcg tgcgcgctaa gatcggcgtc gtcgcgtacg acaagcggat cgatccgatc ggcacgtgcg tcggcatccg cggctcgcgc gtgcaggccg tgcgcaacga gctcggtggc gaaaacatcg acatcgtgct atggtcggag gatcctgccc agttcgtgat cggcgcgctc gcgccggcgg ccgtccagtc gatcgtcgtc gatgaagaaa agcattcgat ggacgtcgtc gtcgacgaga acgaattggc tgtcgcgatc ggccgcagcg gccagaacgt gcgtcttgcc agcgaactga ccggctggca gatcaacatc atgacgccgg acgaatccgc ccagaagcag aacgaagagc gcgacgcgct gcgcggcctg ttcatggcgc gcctcgacgt cgacgaggaa gttgcggaca tcctgatcga cgagggcttc acgagcctcg aagagatcgc ttacgtgccg ctcaacgaaa tgctcgagat cgaggcattc gacgaggata ccgtccacga gctgcgcaac cgctcgcgcg acgcgctgct cacgatggcg atcgcgaacg aggagaaggt cgagacggcc gccctcgatc tgaagagtct cgacggcgtc acgcccgaac tgctcgcgaa gctggccgag cagagcgtgc agacgcgcga cgatctcgcg gagcttgccg tggacgagct ggtcgatatg accggcatgg aagaggaagc cgcgaaggcg ctgatcatga aggcgcgcga acactggttc cagtga
Details for ButhA.17985.a.A1.GE32865
HARVESTED ON: 6/28/2011
SEQUENCED ON: 7/22/2011
EXPECTED MW: 57kDa
OBSERVED MW: 69kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 22
PERCENT COVERAGE: 75
Validated AA Sequence
MSATSSSTSR RAMNRPRSAS RSSFCFWADS SGVMMLICQP VSSLARRTFW PLRPIATANS FSSTTTSIEC FSSSTTIDWT AAGASAPITN WAGSSDHSTM SMFSPPSSLR TACTREPRMP THVPIGSIRL SYATTXILAR TPGSRAAALI SRSPCSISXH FHLEELHQEF GRGARQLDLR AASGAVDXRD VRAHAVAHAQ VFLRNQLVAA QQRLDAARFD DEVALVEALH RARHDFLAAL EVVVQDLLAL RVAHLLQDHL LRGLRADPAE LDRRHRLLDV VADLDAGLLL ARLEQQDLAI RLLEARLVGH DEPTTERLVF TALAIDMYAD LGAFVEQLLG SRREGRFERA QHDVLVDVLL ARQRIHQHQH FATHRTRTLG LSFQG
Validated NT Sequence
ttcatgatca gcgccttcgc ggcttcctct tccatgccgg tcatatcgac cagctcgtcc acggcaagct ccgcgagatc gtcgcgcgtc tgcacgctct gctcggccag cttcgcgagc agttcgggcg tgacgccgtc gagactcttc agatcgaggg cggccgtctc gaccttctcc tcgttcgcga tcgccatcgt gagcagcgcg tcgcgcgagc ggttgcgcag ctcgtggacg gtatcctcgt cgaatgcctc gatctcgagc atttcgttga gcggcacgta agcgatctct tcgaggctcg tgaagccctc gtcgatcagg atgtccgcaa cttcctcgtc gacgtcgagg cgcgccatga acaggccgcg cagcgcgtcg cgctcttcgt tctgcttctg ggcggattcg tccggcgtca tgatgttgat ctgccagccg gtcagttcgc tggcaagacg cacgttctgg ccgctgcggc cgatcgcgac agccaattcg ttctcgtcga cgacgacntc catcgaatgc ttttcttcat cgacgacgat cgactggacg gccgccggcg cgagcgcgcc gatcacgaac tgggcaggat cctccgacca tagcacgatg tcgatgtttt cgccaccgag ctcgttgcgc acggcctgca cgcgcgagcc gcggatgccg acgcacgtgc cgatcggatc gatccgcttg tcgtacgcga cgacgcngat cttagcgcgc acgcccggat cgcgagccgc cgccttgatc tcgagaagcc cctgctcgat ttccnggcac ttccatctcg aagagcttca tcaggaattc gggcgcggtg cgcgacagct cgatctgcgg gccgcgagcg gngcggtcga cnttcgcgat gtacgcgcgc acgcggtcgc ccacgcgcag gttttccttc ggaatcagct ggtcgcggcg caacagcgcc tcgacgcggc ccgattcgac gatgaagttg cccttgtcga ggcgcttcac cgtgcccgtc atgattttct cgccgcgctc gaggtagtcg ttcaggatct gctcgcgctc cgcgtcgcgc accttctgca ggatcacctg cttcgcggcc tgcgcgccga tccggccgaa ctcgatcgac ggcaccggct cctcgatgta gtcgccgacc tcgacgccgg gcttctgctc gcgcgcctcg aacagcagga tctcgcgatc cggctcctgg aggcccgcct cgtcgggcac gacgagccaa cgacggaacg tctcgtgttc accgctctcg cgatcgatat gtacgcggat ctcggcgcct tcgtcgaaca gcttcttgga agccgacgcg agggccgctt cgagcgcgcc cagcacgacg tccttgtcga cgttcttctc gcgcgccagc gcatccacca acatcaacac ttcgcgactc atcgaaccag gaccctgggt ctgagcttcc agggta
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSREVLMLV DALAREKNVD KDVVLGALEA ALASASKKLF DEGAEIRVHI DRESGEHETF RRWLVVPDEA GLQEPDREIL LFEAREQKPG VEVGDYIEEP VPSIEFGRIG AQAAKQVILQ KVRDAEREQI LNDYLERGEK IMTGTVKRLD KGNFIVESGR VEALLRRDQL IPKENLRVGD RVRAYIAKVD RTARGPQIEL SRTAPEFLMK LFEMEVPEIE QGLLEIKAAA RDPGVRAKIG VVAYDKRIDP IGTCVGIRGS RVQAVRNELG GENIDIVLWS EDPAQFVIGA LAPAAVQSIV VDEEKHSMDV VVDENELAVA IGRSGQNVRL ASELTGWQIN IMTPDESAQK QNEERDALRG LFMARLDVDE EVADILIDEG FTSLEEIAYV PLNEMLEIEA FDEDTVHELR NRSRDALLTM AIANEEKVET AALDLKSLDG VTPELLAKLA EQSVQTRDDL AELAVDELVD MTGMEEEAAK ALIMKAREHW FQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagtcgcgaa gtgttgatgt tggtggatgc gctggcgcgc gagaagaacg tcgacaagga cgtcgtgctg ggcgcgctcg aagcggccct cgcgtcggct tccaagaagc tgttcgacga aggcgccgag atccgcgtac atatcgatcg cgagagcggt gaacacgaga cgttccgtcg ttggctcgtc gtgcccgacg aggcgggcct ccaggagccg gatcgcgaga tcctgctgtt cgaggcgcgc gagcagaagc ccggcgtcga ggtcggcgac tacatcgagg agccggtgcc gtcgatcgag ttcggccgga tcggcgcgca ggccgcgaag caggtgatcc tgcagaaggt gcgcgacgcg gagcgcgagc agatcctgaa cgactacctc gagcgcggcg agaaaatcat gacgggcacg gtgaagcgcc tcgacaaggg caacttcatc gtcgaatcgg gccgcgtcga ggcgctgttg cgccgcgacc agctgattcc gaaggaaaac ctgcgcgtgg gcgaccgcgt gcgcgcgtac atcgcgaagg tcgaccgcac cgctcgcggc ccgcagatcg agctgtcgcg caccgcgccc gaattcctga tgaagctctt cgagatggaa gtgccggaaa tcgagcaggg gcttctcgag atcaaggcgg cggctcgcga tccgggcgtg cgcgctaaga tcggcgtcgt cgcgtacgac aagcggatcg atccgatcgg cacgtgcgtc ggcatccgcg gctcgcgcgt gcaggccgtg cgcaacgagc tcggtggcga aaacatcgac atcgtgctat ggtcggagga tcctgcccag ttcgtgatcg gcgcgctcgc gccggcggcc gtccagtcga tcgtcgtcga tgaagaaaag cattcgatgg acgtcgtcgt cgacgagaac gaattggctg tcgcgatcgg ccgcagcggc cagaacgtgc gtcttgccag cgaactgacc ggctggcaga tcaacatcat gacgccggac gaatccgccc agaagcagaa cgaagagcgc gacgcgctgc gcggcctgtt catggcgcgc ctcgacgtcg acgaggaagt tgcggacatc ctgatcgacg agggcttcac gagcctcgaa gagatcgctt acgtgccgct caacgaaatg ctcgagatcg aggcattcga cgaggatacc gtccacgagc tgcgcaaccg ctcgcgcgac gcgctgctca cgatggcgat cgcgaacgag gagaaggtcg agacggccgc cctcgatctg aagagtctcg acggcgtcac gcccgaactg ctcgcgaagc tggccgagca gagcgtgcag acgcgcgacg atctcgcgga gcttgccgtg gacgagctgg tcgatatgac cggcatggaa gaggaagccg cgaaggcgct gatcatgaag gcgcgcgaac actggttcca gtaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for ButhA.17985.a.A1.PW33469
PURIFICATION DATe: 9/30/2011
CONCENTRATION: 27.22mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 100µl
PERCENT IDENTITY: 22
PERCENT COVERAGE: 75
Protocol Notes
notes unavailable
Validated AA Sequence
MSATSSSTSR RAMNRPRSAS RSSFCFWADS SGVMMLICQP VSSLARRTFW PLRPIATANS FSSTTTSIEC FSSSTTIDWT AAGASAPITN WAGSSDHSTM SMFSPPSSLR TACTREPRMP THVPIGSIRL SYATTXILAR TPGSRAAALI SRSPCSISXH FHLEELHQEF GRGARQLDLR AASGAVDXRD VRAHAVAHAQ VFLRNQLVAA QQRLDAARFD DEVALVEALH RARHDFLAAL EVVVQDLLAL RVAHLLQDHL LRGLRADPAE LDRRHRLLDV VADLDAGLLL ARLEQQDLAI RLLEARLVGH DEPTTERLVF TALAIDMYAD LGAFVEQLLG SRREGRFERA QHDVLVDVLL ARQRIHQHQH FATHRTRTLG LSFQG
Validated NT Sequence
ttcatgatca gcgccttcgc ggcttcctct tccatgccgg tcatatcgac cagctcgtcc acggcaagct ccgcgagatc gtcgcgcgtc tgcacgctct gctcggccag cttcgcgagc agttcgggcg tgacgccgtc gagactcttc agatcgaggg cggccgtctc gaccttctcc tcgttcgcga tcgccatcgt gagcagcgcg tcgcgcgagc ggttgcgcag ctcgtggacg gtatcctcgt cgaatgcctc gatctcgagc atttcgttga gcggcacgta agcgatctct tcgaggctcg tgaagccctc gtcgatcagg atgtccgcaa cttcctcgtc gacgtcgagg cgcgccatga acaggccgcg cagcgcgtcg cgctcttcgt tctgcttctg ggcggattcg tccggcgtca tgatgttgat ctgccagccg gtcagttcgc tggcaagacg cacgttctgg ccgctgcggc cgatcgcgac agccaattcg ttctcgtcga cgacgacntc catcgaatgc ttttcttcat cgacgacgat cgactggacg gccgccggcg cgagcgcgcc gatcacgaac tgggcaggat cctccgacca tagcacgatg tcgatgtttt cgccaccgag ctcgttgcgc acggcctgca cgcgcgagcc gcggatgccg acgcacgtgc cgatcggatc gatccgcttg tcgtacgcga cgacgcngat cttagcgcgc acgcccggat cgcgagccgc cgccttgatc tcgagaagcc cctgctcgat ttccnggcac ttccatctcg aagagcttca tcaggaattc gggcgcggtg cgcgacagct cgatctgcgg gccgcgagcg gngcggtcga cnttcgcgat gtacgcgcgc acgcggtcgc ccacgcgcag gttttccttc ggaatcagct ggtcgcggcg caacagcgcc tcgacgcggc ccgattcgac gatgaagttg cccttgtcga ggcgcttcac cgtgcccgtc atgattttct cgccgcgctc gaggtagtcg ttcaggatct gctcgcgctc cgcgtcgcgc accttctgca ggatcacctg cttcgcggcc tgcgcgccga tccggccgaa ctcgatcgac ggcaccggct cctcgatgta gtcgccgacc tcgacgccgg gcttctgctc gcgcgcctcg aacagcagga tctcgcgatc cggctcctgg aggcccgcct cgtcgggcac gacgagccaa cgacggaacg tctcgtgttc accgctctcg cgatcgatat gtacgcggat ctcggcgcct tcgtcgaaca gcttcttgga agccgacgcg agggccgctt cgagcgcgcc cagcacgacg tccttgtcga cgttcttctc gcgcgccagc gcatccacca acatcaacac ttcgcgactc atcgaaccag gaccctgggt ctgagcttcc agggta
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSREVLMLV DALAREKNVD KDVVLGALEA ALASASKKLF DEGAEIRVHI DRESGEHETF RRWLVVPDEA GLQEPDREIL LFEAREQKPG VEVGDYIEEP VPSIEFGRIG AQAAKQVILQ KVRDAEREQI LNDYLERGEK IMTGTVKRLD KGNFIVESGR VEALLRRDQL IPKENLRVGD RVRAYIAKVD RTARGPQIEL SRTAPEFLMK LFEMEVPEIE QGLLEIKAAA RDPGVRAKIG VVAYDKRIDP IGTCVGIRGS RVQAVRNELG GENIDIVLWS EDPAQFVIGA LAPAAVQSIV VDEEKHSMDV VVDENELAVA IGRSGQNVRL ASELTGWQIN IMTPDESAQK QNEERDALRG LFMARLDVDE EVADILIDEG FTSLEEIAYV PLNEMLEIEA FDEDTVHELR NRSRDALLTM AIANEEKVET AALDLKSLDG VTPELLAKLA EQSVQTRDDL AELAVDELVD MTGMEEEAAK ALIMKAREHW FQ
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGTCGCGAA GTGTTGATGT TGGTGGATGC GCTGGCGCGC GAGAAGAACG TCGACAAGGA CGTCGTGCTG GGCGCGCTCG AAGCGGCCCT CGCGTCGGCT TCCAAGAAGC TGTTCGACGA AGGCGCCGAG ATCCGCGTAC ATATCGATCG CGAGAGCGGT GAACACGAGA CGTTCCGTCG TTGGCTCGTC GTGCCCGACG AGGCGGGCCT CCAGGAGCCG GATCGCGAGA TCCTGCTGTT CGAGGCGCGC GAGCAGAAGC CCGGCGTCGA GGTCGGCGAC TACATCGAGG AGCCGGTGCC GTCGATCGAG TTCGGCCGGA TCGGCGCGCA GGCCGCGAAG CAGGTGATCC TGCAGAAGGT GCGCGACGCG GAGCGCGAGC AGATCCTGAA CGACTACCTC GAGCGCGGCG AGAAAATCAT GACGGGCACG GTGAAGCGCC TCGACAAGGG CAACTTCATC GTCGAATCGG GCCGCGTCGA GGCGCTGTTG CGCCGCGACC AGCTGATTCC GAAGGAAAAC CTGCGCGTGG GCGACCGCGT GCGCGCGTAC ATCGCGAAGG TCGACCGCAC CGCTCGCGGC CCGCAGATCG AGCTGTCGCG CACCGCGCCC GAATTCCTGA TGAAGCTCTT CGAGATGGAA GTGCCGGAAA TCGAGCAGGG GCTTCTCGAG ATCAAGGCGG CGGCTCGCGA TCCGGGCGTG CGCGCTAAGA TCGGCGTCGT CGCGTACGAC AAGCGGATCG ATCCGATCGG CACGTGCGTC GGCATCCGCG GCTCGCGCGT GCAGGCCGTG CGCAACGAGC TCGGTGGCGA AAACATCGAC ATCGTGCTAT GGTCGGAGGA TCCTGCCCAG TTCGTGATCG GCGCGCTCGC GCCGGCGGCC GTCCAGTCGA TCGTCGTCGA TGAAGAAAAG CATTCGATGG ACGTCGTCGT CGACGAGAAC GAATTGGCTG TCGCGATCGG CCGCAGCGGC CAGAACGTGC GTCTTGCCAG CGAACTGACC GGCTGGCAGA TCAACATCAT GACGCCGGAC GAATCCGCCC AGAAGCAGAA CGAAGAGCGC GACGCGCTGC GCGGCCTGTT CATGGCGCGC CTCGACGTCG ACGAGGAAGT TGCGGACATC CTGATCGACG AGGGCTTCAC GAGCCTCGAA GAGATCGCTT ACGTGCCGCT CAACGAAATG CTCGAGATCG AGGCATTCGA CGAGGATACC GTCCACGAGC TGCGCAACCG CTCGCGCGAC GCGCTGCTCA CGATGGCGAT CGCGAACGAG GAGAAGGTCG AGACGGCCGC CCTCGATCTG AAGAGTCTCG ACGGCGTCAC GCCCGAACTG CTCGCGAAGC TGGCCGAGCA GAGCGTGCAG ACGCGCGACG ATCTCGCGGA GCTTGCCGTG GACGAGCTGG TCGATATGAC CGGCATGGAA GAGGAAGCCG CGAAGGCGCT GATCATGAAG GCGCGCGAAC ACTGGTTCCA GTAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA