SSGCID: Target Page

ButhA.17983.a

HPr kinase/phosphorylase (HPrK/P) (EC 2.7.11.-) (EC 2.7.4.-) (HPr(Ser) kinase/phosphorylase) (BTH_I0483) (hprK)

CENTER ID:	ButhA.17983.a
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.17983.a.A1.GE32864	full length		1	322

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.17983.a.A1.PW33468	full length		1	322

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.3163
RefSeq:	YP_441040.1
UniProt:	Q2T1A9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDTSSINAQS IFDDNAAMLK LSWLTGHEGW ERGFSADTVA NATSSADLVG HLNLIHPNRI QVLGEAEIDY YQRQTDEDRS RHMAELIALE PPFLVVAGGA AAPPELVLRC TRSSTPLFTT PMSAAAVIDS LRLYMSRILA PRATLHGVFL DILGMGVLLT GDSGLGKSEL GLELISRGHG LVADDAVDFV RLGPDFVEGR CPPLLQNLLE VRGLGLLDIK TIFGETAVRR KMKLKLIVQL VRRPDGEFQR LPLESQTVDV LGLPISKVTI QVAAGRNLAV LVEAAVRNTI LQLRGIDTLR DFMDRQRLAM QDPDSQFPGK LV

NT Sequence

atggatacgt ccagcatcaa cgcccagagc atcttcgacg acaacgcagc catgctgaag ctcagctggc tgacggggca tgaaggctgg gaacgcggtt tttccgccga tacggtcgcg aacgcgacgt cgagcgccga cctcgtcggc cacctgaacc tgatccaccc gaaccggatc caggtgctcg gcgaagccga aatcgactac taccagcgcc agaccgacga agaccgctcg cgccacatgg ccgaactgat cgcgctcgag ccgccgtttc tcgtcgtcgc gggcggcgcc gccgcgccgc ccgagctcgt cctgcgctgc acgcgctcgt cgacgccgct gttcacgacg ccgatgtcgg ccgccgctgt gatcgacagc ctgcgcctgt acatgtcgcg catcctcgcg ccgcgcgcga cgctgcacgg cgtgttcctc gacatcctcg gcatgggcgt gctgctgacg ggcgattcgg gcctcggcaa gagcgagctc ggcctcgagc tgatcagccg cggccacggg cttgtcgccg acgacgcggt cgacttcgtc cgacttggcc ccgatttcgt cgaggggcgc tgcccgccgc ttctgcagaa cctgctcgaa gtgcgcggcc tgggcctcct cgacatcaag acgatcttcg gcgaaaccgc ggtgcgccgg aagatgaagc tcaagctgat cgtccagctc gtgcgccggc ccgacggcga gttccagcgg ctgccgctcg aaagccagac ggtcgacgtg ctcggcctgc cgatcagcaa ggtgacgatc caggtcgcgg cgggccgcaa cctcgcggtg ctcgtcgagg cggcggtgcg caacacgatc ctgcagctgc gcggcatcga cacgctgcgc gacttcatgg accgccagcg cctcgcgatg caggacccgg acagccagtt ccccggcaag ctcgtgtaa

Details for ButhA.17983.a.A1.GE32864

HARVESTED ON:	6/28/2011
SEQUENCED ON:	7/22/2011
EXPECTED MW:	37kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMDTSSIN AQSIFDDNAA MLKLSWLTGH EGWERGFSAD TVANATSSAD LVGHLNLIHP NRIQVLGEAE IDYYQRQTDE DRSRHMAELI ALEPPFLVVA GGAAAPPELV LRCTRSSTPL FTTPMSAAAV IDSLRLYMSR ILAPRATLHG VFLDILGMGV LLTGDSGLGK SELGLELISR GHGLVADDAV DFVRLGPDFV EGRCPPLLQN LLEVRGLGLL DIKTIFGETA VRRKMKLKLI VQLVRRPDGE FQRLPLESQT VDVLGLPISK VTIQVAAGRN LAVLVEAAVR NTILQLRGID TLRDFMDRQR LAMQDPDSQF PGKLV

Validated NT Sequence

ttattacacg agcttgccgg ggaactggct gtccgggtcc tgcatcgcga ggcgctggcg gtccatgaag tcgcgcagcg tgtcgatgcc gcgcagctgc aggatcgtgt tgcgcaccgc cgcctcgacg agcaccgcga ggttgcggcc cgccgcgacc tggatcgtca ccttgctgat cggcaggccg agcacgtcga ccgtctggct ttcgagcggc agccgctgga actcgccgtc gggccggcgc acgagctgga cgatcagctt gagcttcatc ttccggcgca ccgcggtttc gccgaagatc gtcttgatgt cgaggaggcc caggccgcgc acttcgagca ggttctgcag aagcggcggg cagcgcccct cgacgaaatc ggggccaagt cggacgaagt cgaccgcgtc gtcggcgaca agcccgtggc cgcggctgat cagctcgagg ccgagctcgc tcttgccgag gcccgaatcg cccgtcagca gcacgcccat gccgaggatg tcgaggaaca cgccgtgcag cgtcgcgcgc ggcgcgagga tgcgcgacat gtacaggcgc aggctgtcga tcacagcggc ggccgacatc ggcgtcgtga acagcggcgt cgacgagcgc gtgcagcgca ggacgagctc gggcggcgcg gcggcgccgc ccgcgacgac gagaaacggc ggctcgagcg cgatcagttc ggccatgtgg cgcgagcggt cttcgtcggt ctggcgctgg tagtagtcga tttcggcttc gccgagcacc tggatccggt tcgggtggat caggttcagg tggccgacga ggtcggcgct cgacgtcgcg ttcgcgaccg tatcggcgga aaaaccgcgt tcccagcctt catgccccgt cagccagctg agcttcagca tggctgcgtt gtcgtcgaag atgctctggg cgttgatgct ggacgtatcc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMDTSSINAQ SIFDDNAAML KLSWLTGHEG WERGFSADTV ANATSSADLV GHLNLIHPNR IQVLGEAEID YYQRQTDEDR SRHMAELIAL EPPFLVVAGG AAAPPELVLR CTRSSTPLFT TPMSAAAVID SLRLYMSRIL APRATLHGVF LDILGMGVLL TGDSGLGKSE LGLELISRGH GLVADDAVDF VRLGPDFVEG RCPPLLQNLL EVRGLGLLDI KTIFGETAVR RKMKLKLIVQ LVRRPDGEFQ RLPLESQTVD VLGLPISKVT IQVAAGRNLA VLVEAAVRNT ILQLRGIDTL RDFMDRQRLA MQDPDSQFPG KLV

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggatacgtcc agcatcaacg cccagagcat cttcgacgac aacgcagcca tgctgaagct cagctggctg acggggcatg aaggctggga acgcggtttt tccgccgata cggtcgcgaa cgcgacgtcg agcgccgacc tcgtcggcca cctgaacctg atccacccga accggatcca ggtgctcggc gaagccgaaa tcgactacta ccagcgccag accgacgaag accgctcgcg ccacatggcc gaactgatcg cgctcgagcc gccgtttctc gtcgtcgcgg gcggcgccgc cgcgccgccc gagctcgtcc tgcgctgcac gcgctcgtcg acgccgctgt tcacgacgcc gatgtcggcc gccgctgtga tcgacagcct gcgcctgtac atgtcgcgca tcctcgcgcc gcgcgcgacg ctgcacggcg tgttcctcga catcctcggc atgggcgtgc tgctgacggg cgattcgggc ctcggcaaga gcgagctcgg cctcgagctg atcagccgcg gccacgggct tgtcgccgac gacgcggtcg acttcgtccg acttggcccc gatttcgtcg aggggcgctg cccgccgctt ctgcagaacc tgctcgaagt gcgcggcctg ggcctcctcg acatcaagac gatcttcggc gaaaccgcgg tgcgccggaa gatgaagctc aagctgatcg tccagctcgt gcgccggccc gacggcgagt tccagcggct gccgctcgaa agccagacgg tcgacgtgct cggcctgccg atcagcaagg tgacgatcca ggtcgcggcg ggccgcaacc tcgcggtgct cgtcgaggcg gcggtgcgca acacgatcct gcagctgcgc ggcatcgaca cgctgcgcga cttcatggac cgccagcgcc tcgcgatgca ggacccggac agccagttcc ccggcaagct cgtgtaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa

Details for ButhA.17983.a.A1.PW33468

PURIFICATION DATe:	9/9/2011
CONCENTRATION:	30.25mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	6
VIAL VOLUME:	100µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGATACGTCC AGCATCAACG CCCAGAGCAT CTTCGACGAC AACGCAGCCA TGCTGAAGCT CAGCTGGCTG ACGGGGCATG AAGGCTGGGA ACGCGGTTTT TCCGCCGATA CGGTCGCGAA CGCGACGTCG AGCGCCGACC TCGTCGGCCA CCTGAACCTG ATCCACCCGA ACCGGATCCA GGTGCTCGGC GAAGCCGAAA TCGACTACTA CCAGCGCCAG ACCGACGAAG ACCGCTCGCG CCACATGGCC GAACTGATCG CGCTCGAGCC GCCGTTTCTC GTCGTCGCGG GCGGCGCCGC CGCGCCGCCC GAGCTCGTCC TGCGCTGCAC GCGCTCGTCG ACGCCGCTGT TCACGACGCC GATGTCGGCC GCCGCTGTGA TCGACAGCCT GCGCCTGTAC ATGTCGCGCA TCCTCGCGCC GCGCGCGACG CTGCACGGCG TGTTCCTCGA CATCCTCGGC ATGGGCGTGC TGCTGACGGG CGATTCGGGC CTCGGCAAGA GCGAGCTCGG CCTCGAGCTG ATCAGCCGCG GCCACGGGCT TGTCGCCGAC GACGCGGTCG ACTTCGTCCG ACTTGGCCCC GATTTCGTCG AGGGGCGCTG CCCGCCGCTT CTGCAGAACC TGCTCGAAGT GCGCGGCCTG GGCCTCCTCG ACATCAAGAC GATCTTCGGC GAAACCGCGG TGCGCCGGAA GATGAAGCTC AAGCTGATCG TCCAGCTCGT GCGCCGGCCC GACGGCGAGT TCCAGCGGCT GCCGCTCGAA AGCCAGACGG TCGACGTGCT CGGCCTGCCG ATCAGCAAGG TGACGATCCA GGTCGCGGCG GGCCGCAACC TCGCGGTGCT CGTCGAGGCG GCGGTGCGCA ACACGATCCT GCAGCTGCGC GGCATCGACA CGCTGCGCGA CTTCATGGAC CGCCAGCGCC TCGCGATGCA GGACCCGGAC AGCCAGTTCC CCGGCAAGCT CGTGTAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA