ButhA.17982.a

CTP synthase (EC 6.3.4.2) (CTP synthetase) (UTP--ammonia ligase) (BTH_I1892) (pyrG)

CENTER ID: ButhA.17982.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.17982.a.A1.GE32863 full length 1 553
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.17982.a.A1.PW33467 full length 1 553

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.4700
RefSeq: YP_442422.1
UniProt: Q2SXC7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTKYVFVTGG VVSSLGKGIA AASLAAILES RGLKVTLLKL DPYINVDPGT MSPFQHGEVF VTEDGAETDL DLGHYERFIS TKMRKANNFT TGQIYESVIR KERRGDYLGK TVQVIPHITN EIQAFIERGA ASATCGEPDV AIVEIGGTVG DIESLPFLEA ARQMSLRLGR NSACFVHLTL VPFIATAGEL KTKPTQHSVQ KLREIGISPH VLLCRADRPI PDDESKKISL FSNVPEDAVI SVWDVDSIYK IPQMLHDQGL DRLICEELRL DPQPADLRMW SELVEKLQNP KHEVTIGMVG KYVDLTESYK SLIEALRHAS IHTSTKVNIE YIDSEELEAN GTASLAHLDA VLVPGGFGRR GTEGKIAAVR YAREAKVPYL GICLGMQLAV IEFARDVVGL KQANSTEFDP NTPERVVALI TEWYDREGKV EKRTEDSDLG GTMRLGSQRC PIKPGTLAEA IYGKDVNERH RHRYEVNNRF VPQLEAGGLV ISARTPSEDL PEMMELPRAM HPWFVGVQFH PEFTSTPRDG HPLFKSFVQA AIACQQARAG AKA
NT Sequence
atgaccaaat atgttttcgt caccggcggc gtagtttcct ccctcggcaa gggcattgcc gccgcctctc tcgccgcgat cctcgaatcg cgcggtctca aagtcaccct cctcaaactc gatccttaca tcaacgtcga ccccggcacg atgagcccgt ttcagcacgg cgaagtgttc gtgacggaag acggcgcgga gacggatctc gacctcggcc actatgagcg cttcatcagc acgaagatgc gcaaggccaa caacttcacg accggtcaga tctacgaatc ggtgatccgc aaggagcgtc gcggcgatta cctcggcaag accgtccagg tgattccgca catcacgaac gagatccagg cgttcatcga gcgcggcgcg gcgtcggcca cctgcggcga gccggatgtg gccatcgtcg agatcggcgg cacggtgggc gacatcgagt cgctgccgtt cctcgaggcg gcgcgccaga tgagcctgcg cctcggccgc aacagcgcat gcttcgttca cctgacgctc gtgccgttca tcgcgacggc gggcgagctg aagacgaagc cgacccagca cagcgtgcag aagctgcgcg agatcggcat ttcgccgcac gtgctgctct gccgtgccga tcgcccgatt cccgacgacg aatcgaagaa gatctcgctc ttctcgaacg tgccggaaga cgcggtgatc tcggtgtggg acgtcgacag catctataag attccgcaga tgctgcacga ccaggggctc gaccgcctca tctgcgagga gttgcgcctc gatccgcagc cggccgacct gcgcatgtgg tcggagctcg tcgagaagct tcagaatccg aagcacgagg tgacgatcgg gatggtcggc aagtacgtcg acctgacgga gtcgtacaag tcgctgatcg aagcgctgcg tcatgcgtcg atccacacgt cgacgaaggt caacatcgag tacatcgatt cggaagagct cgaggcgaac ggcaccgcga gcctcgctca tctcgacgcc gtgctcgtgc cgggcggctt cggccggcgc ggcaccgaag gcaagatcgc ggccgtgcgc tatgcgcgcg aagcgaaggt gccgtatctc ggcatctgcc tcggcatgca actcgccgtc atcgaattcg cgcgcgacgt cgtcggcctc aagcaggcga acagcaccga gttcgatccg aacacgccgg agcgcgtcgt cgcgctgatc accgagtggt acgaccgcga gggcaaggtc gagaagcgca ccgaggattc cgatctcggc ggcacgatgc ggctcggctc gcagcgctgc ccgatcaagc cgggcacgct cgccgaagcg atttacggca aggacgtgaa cgagcgccac cgtcatcgtt acgaagtcaa caaccgcttc gtgccgcaac tcgaagcggg cggcctcgtg atcagcgcgc gcacgccgag cgaagatctg ccggaaatga tggagctgcc gcgcgcgatg cacccgtggt tcgtcggcgt tcagttccac ccggaattca cgtcgacgcc gcgtgacggc catccgctct tcaagtcgtt cgttcaagcc gcgatcgcgt gtcagcaggc gcgcgccgga gcgaaagcat ga
Details for ButhA.17982.a.A1.GE32863
HARVESTED ON: 6/28/2011
SEQUENCED ON: 7/22/2011
EXPECTED MW: 63kDa
OBSERVED MW: 65kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 95
Validated AA Sequence
MTKYVFVTGG VVSSLGKGIA AASLAAILES RGLKVTLLKL DPYINVDPGT MSPFQHGEVF VTEDGAETDL DLGHYERFIS TKMRKANNFT TGQIYESVIR KERRGDYLGK TVQVIPHITN EIQAFIERGA ASATCGEPDV AIVEIGGTVG DIESLPFLEA ARQMSLRLGR NSACFVHLTL VPFIATAGEL KTKPTQHSVQ KLREIGISPH VLLCRADRPI PDDESKKXSL FSNVPEDAVI SVWDVDSIYX XPQMLHDQGL DRLICEELRL DPQPADLRMW XELVEKLQNP KHEVTIGMVG KYVDXTESYK SLIEALRHAS IHTSTKVNIE YIDSEELEAN GTASLAHLDA VLVPGGFGRR GTEGKIAAVR YAREAKVPYL GICLGMQLAV IEFARDVVGL KQANSTEFDP NTPERVVALI TEWYDREGKV EKRTEDSDLG GTMRLGSQRC PIKPGTLAEA IYGKDVNERH RHRYEVNNRF VPQLEAGGLV ISARTPSEDL PEMMELPRAM HPWFVGVQFH PEFTSTPRDG HPLFKSFVQA AIACQQARAG A
Validated NT Sequence
tcgctccggc gcgcgcctgc tgacacgcga tcgcggcttg aacgaacgac ttgaagagcg gatggccgtc acgcggcgtc gacgtgaatt ccgggtggaa ctgaacgccg acgaaccacg ggtgcatcgc gcgcggcagc tccatcattt ccggcagatc ttcgctcggc gtgcgcgcgc tgatcacgag gccgcccgct tcgagttgcg gcacgaagcg gttgttgact tcgtaacgat gacggtggcg ctcgttcacg tccttgccgt aaatcgcttc ggcgagcgtg cccggcttga tcgggcagcg ctgcgagccg agccgcatcg tgccgccgag atcggaatcc tcggtgcgct tctcgacctt gccctcgcgg tcgtaccact cggtgatcag cgcgacgacg cgctccggcg tgttcggatc gaactcggtg ctgttcgcct gcttgaggcc gacgacgtcg cgcgcgaatt cgatgacggc gagttgcatg ccgaggcaga tgccgagata cggcaccttc gcttcgcgcg catagcgcac ggccgcgatc ttgccttcgg tgccgcgccg gccgaagccg cccggcacga gcacggcgtc gagatgagcg aggctcgcgg tgccgttcgc ctcgagctct tccgaatcga tgtactcgat gttgaccttc gtcgacgtgt ggatcgacgc atgacgcagc gcttcgatca gcgacttgta cgactccgtc nngtcgacgt acttgccgac catcccgatc gtcacctcgt gcttcggatt ctgaagcttc tcgacgagct ccnaccacat gcgcaggtcg gccggctgcg gatcgaggcg caactcctcg cagatgaggc ggtcgagccc ctggtcgtgc agcatctgcg gaancntata gatgctgtcg acgtcccaca ccgagatcac cgcgtcttcn ggcacgttcg agaagagcga gntcttcttc gattcgtcgt cgggaatcgg gcgatcggca cggcagagca gcacgtgcgg cgaaatgccg atctcgcgca gcttctgcac gctgtgctgg gtcggcttcg tcttcagctc gcccgccgtc gcgatgaacg gcacgagcgt caggtgaacg aagcatgcgc tgttgcggcc gaggcgcagg ctcatctggc gcgccgcctc gaggaacggc agcgactcga tgtcgcccac cgtgccgccg atctcgacga tggccacatc cggctcgccg caggtggccg acgccgcgcc gcgctcgatg aacgcctgga tctcgttcgt gatgtgcgga atcacctgga cggtcttgcc gaggtaatcg ccgcgacgct ccttgcggat caccgattcg tagatctgac cggtcgtgaa gttgttggcc ttgcgcatct tcgtgctgat gaagcgctca tagtggccga ggtcgagatc cgtctccgcg ccgtcttccg tcacgaacac ttcgccgtgc tgaaacgggc tcatcgtgcc ggggtcgacg ttgatgtaag gatcgagttt gaggagggtg actttgagac cgcgcgattc gaggatcgcg gcgagagagg cggcggcaat gcccttgccg agggaggaaa ctacgccgcc ggtgacgaaa acatatttgg tcatcgaacc aggaccctgg gtctgagctt ccagggt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTKYVFVTG GVVSSLGKGI AAASLAAILE SRGLKVTLLK LDPYINVDPG TMSPFQHGEV FVTEDGAETD LDLGHYERFI STKMRKANNF TTGQIYESVI RKERRGDYLG KTVQVIPHIT NEIQAFIERG AASATCGEPD VAIVEIGGTV GDIESLPFLE AARQMSLRLG RNSACFVHLT LVPFIATAGE LKTKPTQHSV QKLREIGISP HVLLCRADRP IPDDESKKIS LFSNVPEDAV ISVWDVDSIY KIPQMLHDQG LDRLICEELR LDPQPADLRM WSELVEKLQN PKHEVTIGMV GKYVDLTESY KSLIEALRHA SIHTSTKVNI EYIDSEELEA NGTASLAHLD AVLVPGGFGR RGTEGKIAAV RYAREAKVPY LGICLGMQLA VIEFARDVVG LKQANSTEFD PNTPERVVAL ITEWYDREGK VEKRTEDSDL GGTMRLGSQR CPIKPGTLAE AIYGKDVNER HRHRYEVNNR FVPQLEAGGL VISARTPSED LPEMMELPRA MHPWFVGVQF HPEFTSTPRD GHPLFKSFVQ AAIACQQARA GAKA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccaaatat gttttcgtca ccggcggcgt agtttcctcc ctcggcaagg gcattgccgc cgcctctctc gccgcgatcc tcgaatcgcg cggtctcaaa gtcaccctcc tcaaactcga tccttacatc aacgtcgacc ccggcacgat gagcccgttt cagcacggcg aagtgttcgt gacggaagac ggcgcggaga cggatctcga cctcggccac tatgagcgct tcatcagcac gaagatgcgc aaggccaaca acttcacgac cggtcagatc tacgaatcgg tgatccgcaa ggagcgtcgc ggcgattacc tcggcaagac cgtccaggtg attccgcaca tcacgaacga gatccaggcg ttcatcgagc gcggcgcggc gtcggccacc tgcggcgagc cggatgtggc catcgtcgag atcggcggca cggtgggcga catcgagtcg ctgccgttcc tcgaggcggc gcgccagatg agcctgcgcc tcggccgcaa cagcgcatgc ttcgttcacc tgacgctcgt gccgttcatc gcgacggcgg gcgagctgaa gacgaagccg acccagcaca gcgtgcagaa gctgcgcgag atcggcattt cgccgcacgt gctgctctgc cgtgccgatc gcccgattcc cgacgacgaa tcgaagaaga tctcgctctt ctcgaacgtg ccggaagacg cggtgatctc ggtgtgggac gtcgacagca tctataagat tccgcagatg ctgcacgacc aggggctcga ccgcctcatc tgcgaggagt tgcgcctcga tccgcagccg gccgacctgc gcatgtggtc ggagctcgtc gagaagcttc agaatccgaa gcacgaggtg acgatcggga tggtcggcaa gtacgtcgac ctgacggagt cgtacaagtc gctgatcgaa gcgctgcgtc atgcgtcgat ccacacgtcg acgaaggtca acatcgagta catcgattcg gaagagctcg aggcgaacgg caccgcgagc ctcgctcatc tcgacgccgt gctcgtgccg ggcggcttcg gccggcgcgg caccgaaggc aagatcgcgg ccgtgcgcta tgcgcgcgaa gcgaaggtgc cgtatctcgg catctgcctc ggcatgcaac tcgccgtcat cgaattcgcg cgcgacgtcg tcggcctcaa gcaggcgaac agcaccgagt tcgatccgaa cacgccggag cgcgtcgtcg cgctgatcac cgagtggtac gaccgcgagg gcaaggtcga gaagcgcacc gaggattccg atctcggcgg cacgatgcgg ctcggctcgc agcgctgccc gatcaagccg ggcacgctcg ccgaagcgat ttacggcaag gacgtgaacg agcgccaccg tcatcgttac gaagtcaaca accgcttcgt gccgcaactc gaagcgggcg gcctcgtgat cagcgcgcgc acgccgagcg aagatctgcc ggaaatgatg gagctgccgc gcgcgatgca cccgtggttc gtcggcgttc agttccaccc ggaattcacg tcgacgccgc gtgacggcca tccgctcttc aagtcgttcg ttcaagccgc gatcgcgtgt cagcaggcgc gcgccggagc gaaagcataa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for ButhA.17982.a.A1.PW33467
PURIFICATION DATe: 9/2/2011
CONCENTRATION: 32.24mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 15
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 95
Protocol Notes
notes unavailable
Validated AA Sequence
MTKYVFVTGG VVSSLGKGIA AASLAAILES RGLKVTLLKL DPYINVDPGT MSPFQHGEVF VTEDGAETDL DLGHYERFIS TKMRKANNFT TGQIYESVIR KERRGDYLGK TVQVIPHITN EIQAFIERGA ASATCGEPDV AIVEIGGTVG DIESLPFLEA ARQMSLRLGR NSACFVHLTL VPFIATAGEL KTKPTQHSVQ KLREIGISPH VLLCRADRPI PDDESKKXSL FSNVPEDAVI SVWDVDSIYX XPQMLHDQGL DRLICEELRL DPQPADLRMW XELVEKLQNP KHEVTIGMVG KYVDXTESYK SLIEALRHAS IHTSTKVNIE YIDSEELEAN GTASLAHLDA VLVPGGFGRR GTEGKIAAVR YAREAKVPYL GICLGMQLAV IEFARDVVGL KQANSTEFDP NTPERVVALI TEWYDREGKV EKRTEDSDLG GTMRLGSQRC PIKPGTLAEA IYGKDVNERH RHRYEVNNRF VPQLEAGGLV ISARTPSEDL PEMMELPRAM HPWFVGVQFH PEFTSTPRDG HPLFKSFVQA AIACQQARAG A
Validated NT Sequence
tcgctccggc gcgcgcctgc tgacacgcga tcgcggcttg aacgaacgac ttgaagagcg gatggccgtc acgcggcgtc gacgtgaatt ccgggtggaa ctgaacgccg acgaaccacg ggtgcatcgc gcgcggcagc tccatcattt ccggcagatc ttcgctcggc gtgcgcgcgc tgatcacgag gccgcccgct tcgagttgcg gcacgaagcg gttgttgact tcgtaacgat gacggtggcg ctcgttcacg tccttgccgt aaatcgcttc ggcgagcgtg cccggcttga tcgggcagcg ctgcgagccg agccgcatcg tgccgccgag atcggaatcc tcggtgcgct tctcgacctt gccctcgcgg tcgtaccact cggtgatcag cgcgacgacg cgctccggcg tgttcggatc gaactcggtg ctgttcgcct gcttgaggcc gacgacgtcg cgcgcgaatt cgatgacggc gagttgcatg ccgaggcaga tgccgagata cggcaccttc gcttcgcgcg catagcgcac ggccgcgatc ttgccttcgg tgccgcgccg gccgaagccg cccggcacga gcacggcgtc gagatgagcg aggctcgcgg tgccgttcgc ctcgagctct tccgaatcga tgtactcgat gttgaccttc gtcgacgtgt ggatcgacgc atgacgcagc gcttcgatca gcgacttgta cgactccgtc nngtcgacgt acttgccgac catcccgatc gtcacctcgt gcttcggatt ctgaagcttc tcgacgagct ccnaccacat gcgcaggtcg gccggctgcg gatcgaggcg caactcctcg cagatgaggc ggtcgagccc ctggtcgtgc agcatctgcg gaancntata gatgctgtcg acgtcccaca ccgagatcac cgcgtcttcn ggcacgttcg agaagagcga gntcttcttc gattcgtcgt cgggaatcgg gcgatcggca cggcagagca gcacgtgcgg cgaaatgccg atctcgcgca gcttctgcac gctgtgctgg gtcggcttcg tcttcagctc gcccgccgtc gcgatgaacg gcacgagcgt caggtgaacg aagcatgcgc tgttgcggcc gaggcgcagg ctcatctggc gcgccgcctc gaggaacggc agcgactcga tgtcgcccac cgtgccgccg atctcgacga tggccacatc cggctcgccg caggtggccg acgccgcgcc gcgctcgatg aacgcctgga tctcgttcgt gatgtgcgga atcacctgga cggtcttgcc gaggtaatcg ccgcgacgct ccttgcggat caccgattcg tagatctgac cggtcgtgaa gttgttggcc ttgcgcatct tcgtgctgat gaagcgctca tagtggccga ggtcgagatc cgtctccgcg ccgtcttccg tcacgaacac ttcgccgtgc tgaaacgggc tcatcgtgcc ggggtcgacg ttgatgtaag gatcgagttt gaggagggtg actttgagac cgcgcgattc gaggatcgcg gcgagagagg cggcggcaat gcccttgccg agggaggaaa ctacgccgcc ggtgacgaaa acatatttgg tcatcgaacc aggaccctgg gtctgagctt ccagggt
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTKYVFVTG GVVSSLGKGI AAASLAAILE SRGLKVTLLK LDPYINVDPG TMSPFQHGEV FVTEDGAETD LDLGHYERFI STKMRKANNF TTGQIYESVI RKERRGDYLG KTVQVIPHIT NEIQAFIERG AASATCGEPD VAIVEIGGTV GDIESLPFLE AARQMSLRLG RNSACFVHLT LVPFIATAGE LKTKPTQHSV QKLREIGISP HVLLCRADRP IPDDESKKIS LFSNVPEDAV ISVWDVDSIY KIPQMLHDQG LDRLICEELR LDPQPADLRM WSELVEKLQN PKHEVTIGMV GKYVDLTESY KSLIEALRHA SIHTSTKVNI EYIDSEELEA NGTASLAHLD AVLVPGGFGR RGTEGKIAAV RYAREAKVPY LGICLGMQLA VIEFARDVVG LKQANSTEFD PNTPERVVAL ITEWYDREGK VEKRTEDSDL GGTMRLGSQR CPIKPGTLAE AIYGKDVNER HRHRYEVNNR FVPQLEAGGL VISARTPSED LPEMMELPRA MHPWFVGVQF HPEFTSTPRD GHPLFKSFVQ AAIACQQARA GAKA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACCAAATAT GTTTTCGTCA CCGGCGGCGT AGTTTCCTCC CTCGGCAAGG GCATTGCCGC CGCCTCTCTC GCCGCGATCC TCGAATCGCG CGGTCTCAAA GTCACCCTCC TCAAACTCGA TCCTTACATC AACGTCGACC CCGGCACGAT GAGCCCGTTT CAGCACGGCG AAGTGTTCGT GACGGAAGAC GGCGCGGAGA CGGATCTCGA CCTCGGCCAC TATGAGCGCT TCATCAGCAC GAAGATGCGC AAGGCCAACA ACTTCACGAC CGGTCAGATC TACGAATCGG TGATCCGCAA GGAGCGTCGC GGCGATTACC TCGGCAAGAC CGTCCAGGTG ATTCCGCACA TCACGAACGA GATCCAGGCG TTCATCGAGC GCGGCGCGGC GTCGGCCACC TGCGGCGAGC CGGATGTGGC CATCGTCGAG ATCGGCGGCA CGGTGGGCGA CATCGAGTCG CTGCCGTTCC TCGAGGCGGC GCGCCAGATG AGCCTGCGCC TCGGCCGCAA CAGCGCATGC TTCGTTCACC TGACGCTCGT GCCGTTCATC GCGACGGCGG GCGAGCTGAA GACGAAGCCG ACCCAGCACA GCGTGCAGAA GCTGCGCGAG ATCGGCATTT CGCCGCACGT GCTGCTCTGC CGTGCCGATC GCCCGATTCC CGACGACGAA TCGAAGAAGA TCTCGCTCTT CTCGAACGTG CCGGAAGACG CGGTGATCTC GGTGTGGGAC GTCGACAGCA TCTATAAGAT TCCGCAGATG CTGCACGACC AGGGGCTCGA CCGCCTCATC TGCGAGGAGT TGCGCCTCGA TCCGCAGCCG GCCGACCTGC GCATGTGGTC GGAGCTCGTC GAGAAGCTTC AGAATCCGAA GCACGAGGTG ACGATCGGGA TGGTCGGCAA GTACGTCGAC CTGACGGAGT CGTACAAGTC GCTGATCGAA GCGCTGCGTC ATGCGTCGAT CCACACGTCG ACGAAGGTCA ACATCGAGTA CATCGATTCG GAAGAGCTCG AGGCGAACGG CACCGCGAGC CTCGCTCATC TCGACGCCGT GCTCGTGCCG GGCGGCTTCG GCCGGCGCGG CACCGAAGGC AAGATCGCGG CCGTGCGCTA TGCGCGCGAA GCGAAGGTGC CGTATCTCGG CATCTGCCTC GGCATGCAAC TCGCCGTCAT CGAATTCGCG CGCGACGTCG TCGGCCTCAA GCAGGCGAAC AGCACCGAGT TCGATCCGAA CACGCCGGAG CGCGTCGTCG CGCTGATCAC CGAGTGGTAC GACCGCGAGG GCAAGGTCGA GAAGCGCACC GAGGATTCCG ATCTCGGCGG CACGATGCGG CTCGGCTCGC AGCGCTGCCC GATCAAGCCG GGCACGCTCG CCGAAGCGAT TTACGGCAAG GACGTGAACG AGCGCCACCG TCATCGTTAC GAAGTCAACA ACCGCTTCGT GCCGCAACTC GAAGCGGGCG GCCTCGTGAT CAGCGCGCGC ACGCCGAGCG AAGATCTGCC GGAAATGATG GAGCTGCCGC GCGCGATGCA CCCGTGGTTC GTCGGCGTTC AGTTCCACCC GGAATTCACG TCGACGCCGC GTGACGGCCA TCCGCTCTTC AAGTCGTTCG TTCAAGCCGC GATCGCGTGT CAGCAGGCGC GCGCCGGAGC GAAAGCATAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA