ButhA.17973.a
Uncharacterized ACR, COG1565 superfamily (BTH_I0294)
| CENTER ID: | ButhA.17973.a |
| ORGANISM: | Burkholderia thailandensis E264 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.17973.a.A1.GE32960 | full length | 1 | 410 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.17973.a.A1.PS01383 | full length | 1 | 410 |
Structures
4F3N
DEPOSITED: 5/9/2012
DETERMINATION: XRay
CLONE: ButhA.17973.a.A1.GE32960
PROTEIN: ButhA.17973.a.A1.PS01383
4G67
DEPOSITED: 7/18/2012
DETERMINATION: XRay
CLONE: ButhA.17973.a.A1.GE32960
PROTEIN: ButhA.17973.a.A1.PS01383
Publications by SSGCID
Combining functional and structural genomics to sample the essential Burkholderia structome.
Abendroth J, Armour B, Barrett L, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Fox D, Gallagher LA, Gardberg AS, Gillespie A, Manoil C, Myler PJ, Nakazawa-Hewitt S, Napuli A, Nguyen MT, Patrapuvich R, Phan I, Stacy R, Staker BL, Stewart LJ, Van Voorhis WC
PLoS ONE - 2012
volume 8, issue 1, pages e53851
PMID: 23382856; PMCID: PMC3561365
PDB:
3D63,
3DAH,
3EIZ,
3EJ2,
3EK2,
3EZO,
3F0F,
3FTP,
3GK0,
3GK3,
3GVF,
3GWA,
3GWE,
3IML,
3SZ8,
3T4C,
3TML,
3TMQ,
3TXY,
3U7J,
3UE9,
3UK1,
3UK2,
3UND,
3UPT,
3URR,
3UW1,
3UW2,
3UW3,
3V2I,
3V7N,
3V8H,
3V9O,
3V9P,
3VAV,
4DDO,
4DFE,
4DHE,
4DHK,
4DUT,
4DZ4,
4E4T,
4E51,
4EFI,
4EG0,
4EGJ,
4EK2,
4EQY,
4EWG,
4EX5,
4EXQ,
4F2G,
4F32,
4F3N,
4F3Y,
4F4H,
4F7D,
4FK8,
4FRY,
4G1K,
4G67,
4G6Z,
4GHK,
4H3Y,
4H3Z,
4H4G
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_128534 |
| PATRIC ID: | fig|271848.6.peg.2954 |
| RefSeq: | YP_440852.1 |
| UniProt: | Q2T1U7 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSQVSAGTVI YNAGMNPKAH EPASLPVPGP DALAQSEALA ASLRAEIASA GGWIPFSRYM ERVLYAPGMG YYSGGAQKFG RRADDGSDFV TAPELSPLFA QTLARPVAQA LDASGTRRVM EFGAGTGKLA AGLLTALAAL GVELDEYAIV DLSGELRARQ RETLGAQAPG LAARVRWLDA LPERFEGVVV GNEVLDAMPV RLVAKQARGW CERGVSIDDA GAFVFADRPF ARAEEAARLA GIDADEGYVT ETHDAAVAFV RTVCAMLARG AAFFIDYGFP SHEYYHRQRA QGTLMCHYRH RAHGDPFVYP GLQDITAHVE FSAIHEAGVG AGADLLGYTS QARFLLNAGI TDVLAEIDPS DAQHFLPAAN AVQKLISEAE MGELFKVIAF SRGIDGALDA FARGDRSHTL
NT Sequence
gtgtcgcagg tgtcggcggg gacggtcatt tacaatgccg ggatgaatcc gaaagctcac gaacccgcta gtttacccgt tcccggcccc gacgcgctcg cgcagtccga agcgctcgcc gcttcgctgc gcgccgaaat cgcctcggcg ggcggctgga tcccgttttc acgctacatg gagcgcgtgt tgtacgcgcc gggcatgggc tactacagcg gcggcgcgca gaaattcggc cggcgcgccg acgacggcag cgatttcgtg accgcgcccg agctgtcgcc gctcttcgcg caaacgctcg cgcgccccgt cgcgcaggcg ctcgacgcga gcggcacgcg gcgcgtgatg gagttcggcg cgggcacggg caagctcgcg gcggggttgc tgaccgcgct ggccgcgctc ggcgtcgagc tggacgaata tgcgatcgtc gatctgtccg gcgagctgcg cgcgcgccag cgcgagacgc tcggcgcgca agcgcccggc ctcgccgcgc gcgtgcgctg gctcgatgcg ctgcccgagc gcttcgaggg cgtcgtggtc ggcaatgaag tgctggacgc gatgccggtg cggctcgtcg cgaagcaggc gcgcggctgg tgcgagcgcg gcgtatcgat cgacgatgcg ggcgcgttcg tgttcgccga tcggccgttc gcgcgcgccg aagaggcggc gcggctcgcg gggatcgatg ccgacgaagg ctacgtgacc gagacgcacg atgcggcggt ggcgttcgtc cgcacggtgt gcgcgatgct cgcgcgcggc gccgcgttct tcatcgacta cggctttccg agccacgagt actatcaccg gcagcgcgcg cagggcacgc tgatgtgcca ctaccggcac cgtgcgcacg gcgatccgtt cgtctatccg gggctgcagg acatcaccgc gcacgtcgaa ttcagcgcga tccacgaggc gggcgtcggc gcgggcgcgg atctgctcgg ctatacgtcg caggcgcgtt tcctgctgaa cgcggggatc accgacgtgc tcgccgagat cgacccgtcc gacgcgcagc atttcctgcc cgccgcgaac gcggtgcaga agctgatttc cgaggcggag atgggcgagc tcttcaaggt gatcgctttc tcgcgcggca tcgacggcgc gctcgacgcg ttcgcgcgcg gcgaccgctc gcacacgctg tag
Details for ButhA.17973.a.A1.GE32960
| HARVESTED ON: | 7/12/2011 |
| SEQUENCED ON: | 8/3/2011 |
| EXPECTED MW: | 46kDa |
| OBSERVED MW: | 42kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 95 |
Validated AA Sequence
MVSQVSAGTV IYNAGMNPKA HEPASLPVPG PDALAQSEAL AASLRAEIAS AGGWIPFSRY MERVLYAPGM GYYSGGAQKF GRRADDGSDF VTAPELSPLF AQTLARPVAQ ALDASGTRRV MEFGAGTGKL AAGLLTALAA LGVELDEYAI VDLSGELRAR QRETLGAQAP GLAARVRWLD ALPERFEGVV VGNEVLDAMP VRLVAKQARG WCERGVSIDD AGAFVFADRP FARAEEAARL AGIDADEGYV TETHDAAVAF VRTVCAMLAR GAAFFIDYGF PSHEYYHRQR AQGTLMCHYR HRAHGDPFVY PGLQDITAHV EFSAIHEAGV GAGADLLGYT SQARFLLNAG ITDVLAEIDP SDAQHFLPAA NAVQKLISEA EMGELFKVIA FSRGIDGALD AFARGDRSHT L
Validated NT Sequence
ttattacagc gtgtgcgagc ggtcgccgcg cgcgaacgcg tcgagcgcgc cgtcgatgcc gcgcgagaaa gcgatcacct tgaagagctc gcccatctcc gcctcggaaa tcagcttctg caccgcgttc gcggcgggca ggaaatgctg cgcgtcggac gggtcgatct cggcgagcac gtcggtgatc cccgcgttca gcaggaaacg cgcctgcgac gtatagccga gcagatccgc gcccgcgccg acgcccgcct cgtggatcgc gctgaattcg acgtgcgcgg tgatgtcctg cagccccgga tagacgaacg gatcgccgtg cgcacggtgc cggtagtggc acatcagcgt gccctgcgcg cgctgccggt gatagtactc gtggctcgga aagccgtagt cgatgaagaa cgcggcgccg cgcgcgagca tcgcgcacac cgtgcggacg aacgccaccg ccgcatcgtg cgtctcggtc acgtagcctt cgtcggcatc gatccccgcg agccgcgccg cctcttcggc gcgcgcgaac ggccgatcgg cgaacacgaa cgcgcccgca tcgtcgatcg atacgccgcg ctcgcaccag ccgcgcgcct gcttcgcgac gagccgcacc ggcatcgcgt ccagcacttc attgccgacc acgacgccct cgaagcgctc gggcagcgca tcgagccagc gcacgcgcgc ggcgaggccg ggcgcttgcg cgccgagcgt ctcgcgctgg cgcgcgcgca gctcgccgga cagatcgacg atcgcatatt cgtccagctc gacgccgagc gcggccagcg cggtcagcaa ccccgccgcg agcttgcccg tgcccgcgcc gaactccatc acgcgccgcg tgccgctcgc gtcgagcgcc tgcgcgacgg ggcgcgcgag cgtttgcgcg aagagcggcg acagctcggg cgcggtcacg aaatcgctgc cgtcgtcggc gcgccggccg aatttctgcg cgccgccgct gtagtagccc atgcccggcg cgtacaacac gcgctccatg tagcgtgaaa acgggatcca gccgcccgcc gaggcgattt cggcgcgcag cgaagcggcg agcgcttcgg actgcgcgag cgcgtcgggg ccgggaacgg gtaaactagc gggttcgtga gctttcggat tcatcccggc attgtaaatg accgtccccg ccgacacctg cgacaccatc gaaccaggac cctgggtctg agcttccagg gt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSQVSAGT VIYNAGMNPK AHEPASLPVP GPDALAQSEA LAASLRAEIA SAGGWIPFSR YMERVLYAPG MGYYSGGAQK FGRRADDGSD FVTAPELSPL FAQTLARPVA QALDASGTRR VMEFGAGTGK LAAGLLTALA ALGVELDEYA IVDLSGELRA RQRETLGAQA PGLAARVRWL DALPERFEGV VVGNEVLDAM PVRLVAKQAR GWCERGVSID DAGAFVFADR PFARAEEAAR LAGIDADEGY VTETHDAAVA FVRTVCAMLA RGAAFFIDYG FPSHEYYHRQ RAQGTLMCHY RHRAHGDPFV YPGLQDITAH VEFSAIHEAG VGAGADLLGY TSQARFLLNA GITDVLAEID PSDAQHFLPA ANAVQKLISE AEMGELFKVI AFSRGIDGAL DAFARGDRSH TL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgtcgcag gtgtcggcgg ggacggtcat ttacaatgcc gggatgaatc cgaaagctca cgaacccgct agtttacccg ttcccggccc cgacgcgctc gcgcagtccg aagcgctcgc cgcttcgctg cgcgccgaaa tcgcctcggc gggcggctgg atcccgtttt cacgctacat ggagcgcgtg ttgtacgcgc cgggcatggg ctactacagc ggcggcgcgc agaaattcgg ccggcgcgcc gacgacggca gcgatttcgt gaccgcgccc gagctgtcgc cgctcttcgc gcaaacgctc gcgcgccccg tcgcgcaggc gctcgacgcg agcggcacgc ggcgcgtgat ggagttcggc gcgggcacgg gcaagctcgc ggcggggttg ctgaccgcgc tggccgcgct cggcgtcgag ctggacgaat atgcgatcgt cgatctgtcc ggcgagctgc gcgcgcgcca gcgcgagacg ctcggcgcgc aagcgcccgg cctcgccgcg cgcgtgcgct ggctcgatgc gctgcccgag cgcttcgagg gcgtcgtggt cggcaatgaa gtgctggacg cgatgccggt gcggctcgtc gcgaagcagg cgcgcggctg gtgcgagcgc ggcgtatcga tcgacgatgc gggcgcgttc gtgttcgccg atcggccgtt cgcgcgcgcc gaagaggcgg cgcggctcgc ggggatcgat gccgacgaag gctacgtgac cgagacgcac gatgcggcgg tggcgttcgt ccgcacggtg tgcgcgatgc tcgcgcgcgg cgccgcgttc ttcatcgact acggctttcc gagccacgag tactatcacc ggcagcgcgc gcagggcacg ctgatgtgcc actaccggca ccgtgcgcac ggcgatccgt tcgtctatcc ggggctgcag gacatcaccg cgcacgtcga attcagcgcg atccacgagg cgggcgtcgg cgcgggcgcg gatctgctcg gctatacgtc gcaggcgcgt ttcctgctga acgcggggat caccgacgtg ctcgccgaga tcgacccgtc cgacgcgcag catttcctgc ccgccgcgaa cgcggtgcag aagctgattt ccgaggcgga gatgggcgag ctcttcaagg tgatcgcttt ctcgcgcggc atcgacggcg cgctcgacgc gttcgcgcgc ggcgaccgct cgcacacgct gtaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for ButhA.17973.a.A1.PS01383
| PURIFICATION DATe: | 2/14/2012 |
| CONCENTRATION: | 86.15mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 95 |
Protocol Notes
notes unavailable
Validated AA Sequence
MVSQVSAGTV IYNAGMNPKA HEPASLPVPG PDALAQSEAL AASLRAEIAS AGGWIPFSRY MERVLYAPGM GYYSGGAQKF GRRADDGSDF VTAPELSPLF AQTLARPVAQ ALDASGTRRV MEFGAGTGKL AAGLLTALAA LGVELDEYAI VDLSGELRAR QRETLGAQAP GLAARVRWLD ALPERFEGVV VGNEVLDAMP VRLVAKQARG WCERGVSIDD AGAFVFADRP FARAEEAARL AGIDADEGYV TETHDAAVAF VRTVCAMLAR GAAFFIDYGF PSHEYYHRQR AQGTLMCHYR HRAHGDPFVY PGLQDITAHV EFSAIHEAGV GAGADLLGYT SQARFLLNAG ITDVLAEIDP SDAQHFLPAA NAVQKLISEA EMGELFKVIA FSRGIDGALD AFARGDRSHT L
Validated NT Sequence
ttattacagc gtgtgcgagc ggtcgccgcg cgcgaacgcg tcgagcgcgc cgtcgatgcc gcgcgagaaa gcgatcacct tgaagagctc gcccatctcc gcctcggaaa tcagcttctg caccgcgttc gcggcgggca ggaaatgctg cgcgtcggac gggtcgatct cggcgagcac gtcggtgatc cccgcgttca gcaggaaacg cgcctgcgac gtatagccga gcagatccgc gcccgcgccg acgcccgcct cgtggatcgc gctgaattcg acgtgcgcgg tgatgtcctg cagccccgga tagacgaacg gatcgccgtg cgcacggtgc cggtagtggc acatcagcgt gccctgcgcg cgctgccggt gatagtactc gtggctcgga aagccgtagt cgatgaagaa cgcggcgccg cgcgcgagca tcgcgcacac cgtgcggacg aacgccaccg ccgcatcgtg cgtctcggtc acgtagcctt cgtcggcatc gatccccgcg agccgcgccg cctcttcggc gcgcgcgaac ggccgatcgg cgaacacgaa cgcgcccgca tcgtcgatcg atacgccgcg ctcgcaccag ccgcgcgcct gcttcgcgac gagccgcacc ggcatcgcgt ccagcacttc attgccgacc acgacgccct cgaagcgctc gggcagcgca tcgagccagc gcacgcgcgc ggcgaggccg ggcgcttgcg cgccgagcgt ctcgcgctgg cgcgcgcgca gctcgccgga cagatcgacg atcgcatatt cgtccagctc gacgccgagc gcggccagcg cggtcagcaa ccccgccgcg agcttgcccg tgcccgcgcc gaactccatc acgcgccgcg tgccgctcgc gtcgagcgcc tgcgcgacgg ggcgcgcgag cgtttgcgcg aagagcggcg acagctcggg cgcggtcacg aaatcgctgc cgtcgtcggc gcgccggccg aatttctgcg cgccgccgct gtagtagccc atgcccggcg cgtacaacac gcgctccatg tagcgtgaaa acgggatcca gccgcccgcc gaggcgattt cggcgcgcag cgaagcggcg agcgcttcgg actgcgcgag cgcgtcgggg ccgggaacgg gtaaactagc gggttcgtga gctttcggat tcatcccggc attgtaaatg accgtccccg ccgacacctg cgacaccatc gaaccaggac cctgggtctg agcttccagg gt
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSQVSAGT VIYNAGMNPK AHEPASLPVP GPDALAQSEA LAASLRAEIA SAGGWIPFSR YMERVLYAPG MGYYSGGAQK FGRRADDGSD FVTAPELSPL FAQTLARPVA QALDASGTRR VMEFGAGTGK LAAGLLTALA ALGVELDEYA IVDLSGELRA RQRETLGAQA PGLAARVRWL DALPERFEGV VVGNEVLDAM PVRLVAKQAR GWCERGVSID DAGAFVFADR PFARAEEAAR LAGIDADEGY VTETHDAAVA FVRTVCAMLA RGAAFFIDYG FPSHEYYHRQ RAQGTLMCHY RHRAHGDPFV YPGLQDITAH VEFSAIHEAG VGAGADLLGY TSQARFLLNA GITDVLAEID PSDAQHFLPA ANAVQKLISE AEMGELFKVI AFSRGIDGAL DAFARGDRSH TL
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGTCGCAG GTGTCGGCGG GGACGGTCAT TTACAATGCC GGGATGAATC CGAAAGCTCA CGAACCCGCT AGTTTACCCG TTCCCGGCCC CGACGCGCTC GCGCAGTCCG AAGCGCTCGC CGCTTCGCTG CGCGCCGAAA TCGCCTCGGC GGGCGGCTGG ATCCCGTTTT CACGCTACAT GGAGCGCGTG TTGTACGCGC CGGGCATGGG CTACTACAGC GGCGGCGCGC AGAAATTCGG CCGGCGCGCC GACGACGGCA GCGATTTCGT GACCGCGCCC GAGCTGTCGC CGCTCTTCGC GCAAACGCTC GCGCGCCCCG TCGCGCAGGC GCTCGACGCG AGCGGCACGC GGCGCGTGAT GGAGTTCGGC GCGGGCACGG GCAAGCTCGC GGCGGGGTTG CTGACCGCGC TGGCCGCGCT CGGCGTCGAG CTGGACGAAT ATGCGATCGT CGATCTGTCC GGCGAGCTGC GCGCGCGCCA GCGCGAGACG CTCGGCGCGC AAGCGCCCGG CCTCGCCGCG CGCGTGCGCT GGCTCGATGC GCTGCCCGAG CGCTTCGAGG GCGTCGTGGT CGGCAATGAA GTGCTGGACG CGATGCCGGT GCGGCTCGTC GCGAAGCAGG CGCGCGGCTG GTGCGAGCGC GGCGTATCGA TCGACGATGC GGGCGCGTTC GTGTTCGCCG ATCGGCCGTT CGCGCGCGCC GAAGAGGCGG CGCGGCTCGC GGGGATCGAT GCCGACGAAG GCTACGTGAC CGAGACGCAC GATGCGGCGG TGGCGTTCGT CCGCACGGTG TGCGCGATGC TCGCGCGCGG CGCCGCGTTC TTCATCGACT ACGGCTTTCC GAGCCACGAG TACTATCACC GGCAGCGCGC GCAGGGCACG CTGATGTGCC ACTACCGGCA CCGTGCGCAC GGCGATCCGT TCGTCTATCC GGGGCTGCAG GACATCACCG CGCACGTCGA ATTCAGCGCG ATCCACGAGG CGGGCGTCGG CGCGGGCGCG GATCTGCTCG GCTATACGTC GCAGGCGCGT TTCCTGCTGA ACGCGGGGAT CACCGACGTG CTCGCCGAGA TCGACCCGTC CGACGCGCAG CATTTCCTGC CCGCCGCGAA CGCGGTGCAG AAGCTGATTT CCGAGGCGGA GATGGGCGAG CTCTTCAAGG TGATCGCTTT CTCGCGCGGC ATCGACGGCG CGCTCGACGC GTTCGCGCGC GGCGACCGCT CGCACACGCT GTAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA