SSGCID: Target Page

ButhA.17963.a

UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (EC 2.4.1.227) (Undecaprenyl-PP-MurNAc-pentapeptide-UDPGlcNAc GlcNAc transferase) (BTH_I1118) (murG)

CENTER ID:	ButhA.17963.a
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.17963.a.A1.GE32952	full length		1	367

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.17963.a.A1.PS01280	full length		1	367

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.3874
RefSeq:	YP_441666.1
UniProt:	Q2SZI3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTSTPRTLMV MAGGTGGHVF PGLAVAHRMQ AQGWRVVWLG NPAGMEATLV PKHGIPMEYV RFGGLRGKGL ATKLALPFNL LRACAQSLRA LRRVKPDVVL GMGGYITFPA GLVTVLTGRP LVLHEQNSIA GLTNKVLAKL AKRVLVAFPG ALPNAEWTGN PIRAELARTE PPQARYAARS GKLKLLVVGG SLGAAALNEV VPRALALLAP SERPQVVHQA GAKHIDVLKE NYEAAGLACG SDVELVPFID DMASAYASAD LVICRSGAMT VAEIAAVGVA ALFVPFPHAV DDHQTTNAEF LAEQGAAVLV QQRDLSAELL ADWLRGQSRE SLAAMAERSR SLAKPDATDE VARVCAAVAG ANLEGKQ

NT Sequence

atgacctcga cgccgcgcac actgatggtg atggcgggcg gcaccggggg gcacgtgttc cccgggctcg cggtcgcgca ccggatgcag gcgcagggct ggcgcgtcgt gtggctcggc aatccggccg gcatggaagc gacgctcgtg cccaagcacg ggattccgat ggagtacgtg cgcttcggcg ggctgcgcgg caagggcctc gcgacgaagc ttgcgctgcc gttcaacctg ctgcgcgcat gcgcgcagag cctgcgcgcg ctgcgccgcg tgaagccgga cgtcgtgctc ggcatgggcg gctacatcac gttcccggcc ggcctcgtga ccgtgctgac gggccggccg ctcgtgctgc acgagcagaa ttcgatcgcc ggcctgacga acaaggtgct cgcgaagctc gcgaagcgcg tgctcgtcgc gttccccggc gcgctgccga acgccgaatg gacaggcaat ccgattcgcg cggaacttgc gcgcacggaa ccgccccaag cacgctatgc ggcgcgcagc gggaagctga agctgctcgt cgtcggcggc agtctcggcg cggccgcgct gaacgaagtc gtgccgcgcg cgctcgcgct tctcgcgccg agcgagcggc cgcaggtcgt gcatcaggcg ggcgcgaaac acatcgacgt gctgaaggag aactacgaag ccgcgggcct ggcttgcggc agcgacgtcg aactcgtgcc gttcattgac gacatggcgt ccgcgtacgc gagcgcggat ctcgtgatct gccgttcggg cgcgatgacg gtcgcggaga tcgcggcggt cggcgtcgcg gcgctgttcg tgccgttccc gcacgccgtc gacgatcatc agacgaccaa cgccgagttc cttgccgagc aaggcgcggc ggtgctcgtg caacaacgcg acctgtcggc cgagctgctc gccgactggc tgcgcggcca gtcgcgggaa tcgctcgcgg cgatggccga acgttcgcgc tcgctcgcga agccggatgc caccgacgaa gtcgcgcgcg tgtgcgcggc ggtggcgggc gcgaacctgg aaggaaagca atga

Details for ButhA.17963.a.A1.GE32952

HARVESTED ON:	7/12/2011
SEQUENCED ON:	8/3/2011
EXPECTED MW:	40kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	96
PERCENT COVERAGE:	94

Validated AA Sequence

MTSTPRTLMV MAGGTGGHVF PGLAVAHRMQ AQGWRVVWLG NPAGMEATLV PKHGIPMEYV RFGGLRGKGL ATKLALPFNL LRACAQSLRA LRRVKPDVVL GMGGYITFPA GLVTVLTGRP LVLHEQNSIA GLTNKVLAKL AKRVLVAFPG ALPNAEWTGN PIRAELARTE PPQARYAARS GKLKLLVVGG SLGAAALNEV VPRALALLAP SERPQVVHQA GAKHIDVLKE NYEAAGLACG SDVELVPFID DMASAYASAD LVICRSGAMT VAEIAAVGVA ALFVPFPHAV DDHQTTNAEF LAEQGXAVLV QQRDLSXXXL AXXXRGQSXX SLAAMAERSR SLAKPXATXE VARVXAAVAG XNLEGKQ

Validated NT Sequence

attattgctt tccttccagg ttcncgcccg ccaccgccgc ncnnacgcgc gcgacttcnt cggtggcntc cggcttcgcg agcgagcgcg aacgttcggc catcgccgcg agcgantnnn gcgactggcc gcgcancnng ncggcgagna nntcgnccga caggtcgcgt tgttgcacga gcaccgccnc gccttgctcg gcaaggaact cggcgttggt cgtctgatga tcgtcgacgg cgtgcgggaa cggcacgaac agcgccgcga cgccgaccgc cgcgatctcc gcgaccgtca tcgcgcccga acggcagatc acgagatccg cgctcgcgta cgcggacgcc atgtcgtcaa tgaacggcac gagttcgacg tcgctgccgc aagccaggcc cgcggcttcg tagttctcct tcagcacgtc gatgtgtttc gcgcccgcct gatgcacgac ctgcggccgc tcgctcggcg cgagaagcgc gagcgcgcgc ggcacgactt cgttcagcgc ggccgcgccg agactgccgc cgacgacgag cagcttcagc ttcccgctgc gcgccgcata gcgtgcttgg ggcggttccg tgcgcgcaag ttccgcgcga atcggattgc ctgtccattc ggcgttcggc agcgcgccgg ggaacgcgac gagcacgcgc ttcgcgagct tcgcgagcac cttgttcgtc aggccggcga tcgaattctg ctcgtgcagc acgagcggcc ggcccgtcag cacggtcacg aggccggccg ggaacgtgat gtagccgccc atgccgagca cgacgtccgg cttcacgcgg cgcagcgcgc gcaggctctg cgcgcatgcg cgcagcaggt tgaacggcag cgcaagcttc gtcgcgaggc ccttgccgcg cagcccgccg aagcgcacgt actccatcgg aatcccgtgc ttgggcacga gcgtcgcttc catgccggcc ggattgccga gccacacgac gcgccagccc tgcgcctgca tccggtgcgc gaccgcgagc ccggggaaca cgtgcccccc ggtgccgccc gccatcacca tcagtgtgcg cggcgtcgag gtcatcgaac caggaccctg ggtctgagct tccag

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMTSTPRTLM VMAGGTGGHV FPGLAVAHRM QAQGWRVVWL GNPAGMEATL VPKHGIPMEY VRFGGLRGKG LATKLALPFN LLRACAQSLR ALRRVKPDVV LGMGGYITFP AGLVTVLTGR PLVLHEQNSI AGLTNKVLAK LAKRVLVAFP GALPNAEWTG NPIRAELART EPPQARYAAR SGKLKLLVVG GSLGAAALNE VVPRALALLA PSERPQVVHQ AGAKHIDVLK ENYEAAGLAC GSDVELVPFI DDMASAYASA DLVICRSGAM TVAEIAAVGV AALFVPFPHA VDDHQTTNAE FLAEQGAAVL VQQRDLSAEL LADWLRGQSR ESLAAMAERS RSLAKPDATD EVARVCAAVA GANLEGKQ

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacctcgacg ccgcgcacac tgatggtgat ggcgggcggc accggggggc acgtgttccc cgggctcgcg gtcgcgcacc ggatgcaggc gcagggctgg cgcgtcgtgt ggctcggcaa tccggccggc atggaagcga cgctcgtgcc caagcacggg attccgatgg agtacgtgcg cttcggcggg ctgcgcggca agggcctcgc gacgaagctt gcgctgccgt tcaacctgct gcgcgcatgc gcgcagagcc tgcgcgcgct gcgccgcgtg aagccggacg tcgtgctcgg catgggcggc tacatcacgt tcccggccgg cctcgtgacc gtgctgacgg gccggccgct cgtgctgcac gagcagaatt cgatcgccgg cctgacgaac aaggtgctcg cgaagctcgc gaagcgcgtg ctcgtcgcgt tccccggcgc gctgccgaac gccgaatgga caggcaatcc gattcgcgcg gaacttgcgc gcacggaacc gccccaagca cgctatgcgg cgcgcagcgg gaagctgaag ctgctcgtcg tcggcggcag tctcggcgcg gccgcgctga acgaagtcgt gccgcgcgcg ctcgcgcttc tcgcgccgag cgagcggccg caggtcgtgc atcaggcggg cgcgaaacac atcgacgtgc tgaaggagaa ctacgaagcc gcgggcctgg cttgcggcag cgacgtcgaa ctcgtgccgt tcattgacga catggcgtcc gcgtacgcga gcgcggatct cgtgatctgc cgttcgggcg cgatgacggt cgcggagatc gcggcggtcg gcgtcgcggc gctgttcgtg ccgttcccgc acgccgtcga cgatcatcag acgaccaacg ccgagttcct tgccgagcaa ggcgcggcgg tgctcgtgca acaacgcgac ctgtcggccg agctgctcgc cgactggctg cgcggccagt cgcgggaatc gctcgcggcg atggccgaac gttcgcgctc gctcgcgaag ccggatgcca ccgacgaagt cgcgcgcgtg tgcgcggcgg tggcgggcgc gaacctggaa ggaaagcaat aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa

Details for ButhA.17963.a.A1.PS01280

PURIFICATION DATe:	11/17/2011
CONCENTRATION:	40.44mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	6
VIAL VOLUME:	200µl
PERCENT IDENTITY:	96
PERCENT COVERAGE:	94

Protocol Notes

notes unavailable

Validated AA Sequence

MTSTPRTLMV MAGGTGGHVF PGLAVAHRMQ AQGWRVVWLG NPAGMEATLV PKHGIPMEYV RFGGLRGKGL ATKLALPFNL LRACAQSLRA LRRVKPDVVL GMGGYITFPA GLVTVLTGRP LVLHEQNSIA GLTNKVLAKL AKRVLVAFPG ALPNAEWTGN PIRAELARTE PPQARYAARS GKLKLLVVGG SLGAAALNEV VPRALALLAP SERPQVVHQA GAKHIDVLKE NYEAAGLACG SDVELVPFID DMASAYASAD LVICRSGAMT VAEIAAVGVA ALFVPFPHAV DDHQTTNAEF LAEQGXAVLV QQRDLSXXXL AXXXRGQSXX SLAAMAERSR SLAKPXATXE VARVXAAVAG XNLEGKQ

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACCTCGACG CCGCGCACAC TGATGGTGAT GGCGGGCGGC ACCGGGGGGC ACGTGTTCCC CGGGCTCGCG GTCGCGCACC GGATGCAGGC GCAGGGCTGG CGCGTCGTGT GGCTCGGCAA TCCGGCCGGC ATGGAAGCGA CGCTCGTGCC CAAGCACGGG ATTCCGATGG AGTACGTGCG CTTCGGCGGG CTGCGCGGCA AGGGCCTCGC GACGAAGCTT GCGCTGCCGT TCAACCTGCT GCGCGCATGC GCGCAGAGCC TGCGCGCGCT GCGCCGCGTG AAGCCGGACG TCGTGCTCGG CATGGGCGGC TACATCACGT TCCCGGCCGG CCTCGTGACC GTGCTGACGG GCCGGCCGCT CGTGCTGCAC GAGCAGAATT CGATCGCCGG CCTGACGAAC AAGGTGCTCG CGAAGCTCGC GAAGCGCGTG CTCGTCGCGT TCCCCGGCGC GCTGCCGAAC GCCGAATGGA CAGGCAATCC GATTCGCGCG GAACTTGCGC GCACGGAACC GCCCCAAGCA CGCTATGCGG CGCGCAGCGG GAAGCTGAAG CTGCTCGTCG TCGGCGGCAG TCTCGGCGCG GCCGCGCTGA ACGAAGTCGT GCCGCGCGCG CTCGCGCTTC TCGCGCCGAG CGAGCGGCCG CAGGTCGTGC ATCAGGCGGG CGCGAAACAC ATCGACGTGC TGAAGGAGAA CTACGAAGCC GCGGGCCTGG CTTGCGGCAG CGACGTCGAA CTCGTGCCGT TCATTGACGA CATGGCGTCC GCGTACGCGA GCGCGGATCT CGTGATCTGC CGTTCGGGCG CGATGACGGT CGCGGAGATC GCGGCGGTCG GCGTCGCGGC GCTGTTCGTG CCGTTCCCGC ACGCCGTCGA CGATCATCAG ACGACCAACG CCGAGTTCCT TGCCGAGCAA GGCGCGGCGG TGCTCGTGCA ACAACGCGAC CTGTCGGCCG AGCTGCTCGC CGACTGGCTG CGCGGCCAGT CGCGGGAATC GCTCGCGGCG ATGGCCGAAC GTTCGCGCTC GCTCGCGAAG CCGGATGCCA CCGACGAAGT CGCGCGCGTG TGCGCGGCGG TGGCGGGCGC GAACCTGGAA GGAAAGCAAT AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA