ButhA.17954.a

Lipid-A-disaccharide synthase (EC 2.4.1.182) (BTH_I2040) (lpxB)

CENTER ID: ButhA.17954.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.17954.a.A1.GE32853 full length 1 388
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.4850
RefSeq: YP_442564.1
UniProt: Q2SWY5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAFQPTPLRV ALVAGEPSGD LLGASLLGGL HAQLPASSRY YGIGGPRMTA VDFDAHWPME KLAVRGYVEA LKHIPEILRI RGELKRQLLA EPPDAFIGID APDFNFGLEQ ALRGAGIPTV HFVCPSIWAW RGGRIKKIVK AVDHMLCLFP FEPELLEKAG VAATFVGHPL ADEIPLEPDM HGARIALGLP DSGPVIAVLP GSRRSEIELI GPTFFDAMAL MQQREPGVRF VVPAATPALR ELLQPLVDAH PLLSVTLTEG RAQVAMTAAD AILVKSGTVT LEAALLKKPM VISYKVPWLT GQIMRRQGYL PYVGLPNILA GRFVVPELLQ HFATPEALAD ATLTQLRDDA NRRTLTGIFT DMHLALRQNT AQRAAEAVAR VIDSRKPH
NT Sequence
atggcgttcc aacccacacc gctgcgcgtc gcgctcgtcg cgggcgaacc gtcgggcgac ctgctcggcg cgtcgctcct cggcgggctg cacgcgcagt tgcccgcatc gtcccgctat tacgggatcg gcgggccgcg catgaccgcc gtggatttcg acgcgcactg gccgatggaa aagctcgcgg tgcgcggcta cgtcgaggcg ctcaagcaca ttcccgagat cctgcgcatt cgcggcgaac tcaagcgcca gttgctcgct gaaccgcccg acgcgttcat cggcatcgac gcgcccgatt tcaacttcgg cctcgagcag gcgctgcgcg gcgccggcat tccgacggtc catttcgtct gcccgtcgat ctgggcatgg cgcggcggcc ggatcaagaa gatcgtcaag gccgtcgacc acatgctgtg cctgttcccg ttcgagcccg aactgctcga gaaggccggc gtcgcggcga cgttcgtcgg ccatccgctc gccgacgaga ttccgctcga acccgacatg cacggcgcgc gaatcgcgct cggcctgccg gatagcggcc ccgtgatcgc ggtgctgccg ggcagccgcc gctcggagat cgagctgatc ggcccgacgt tcttcgacgc gatggcgttg atgcagcagc gcgagcccgg cgtgcgcttc gtcgtgccgg ccgcgacgcc cgcgctgcgc gagttgctgc agccgctcgt cgacgcgcac ccgttgctgt cggtcacgct gaccgaaggc cgcgcgcagg tcgcgatgac ggcggccgat gcgatcctcg tgaagagcgg caccgtgacg ctcgaagcgg cgctgctcaa gaaaccgatg gtgatctcgt acaaggtgcc gtggctcacg gggcagatca tgcgccggca gggctatctg ccgtacgtcg gattgccgaa catcctcgcg ggccgtttcg tcgtgcctga gctgctgcag catttcgcga cgcccgaggc gctcgccgac gcgacgctca cgcaactgcg cgacgatgcg aaccgccgca cgctcactgg tattttcacc gacatgcatc tcgcgttgcg ccagaacacc gcgcagcgcg cggccgaggc cgtcgcgcgc gtgatcgaca gcaggaagcc gcactga
Details for ButhA.17954.a.A1.GE32853
HARVESTED ON: 6/28/2011
SEQUENCED ON: 7/22/2011
EXPECTED MW: 44kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 74
PERCENT COVERAGE: 94
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAFQPTPLR VALVAGEPSG DLLGASLLGG LHAQLPASSR YYGIGGPRMT AVDFDAHWPM EKLAVRGYVE ALKHIPEILR IRGELKRQLL AEPPDAFIGI DAPDFNFGLE QALRGAGIPT VHFVCPSIWA WRGGRIKKIV KAVDHMLCLF PFEPELLEKA GVAATFVGHP LADEIPLEPD MHGARIALGL PDSGPVIAVL PGSRRSEIEL IGPTFFDAMA LMQQREPGVR FVVPAATPAL RELLQPLVDA HPLLSVTLTE RXRAGRDDXG RCDPREXRXR DXRSXAAXET DGDXVXXXXR GXSXXXRXIC RTXXXXTXXX XXSSXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXX
Validated NT Sequence
attttnttta ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcgt tccaacccac accgctgcgc gtcgcgctcg tcgcgggcga accgtcgggc gacctgctcg gcgcgtcgct cctcggcggg ctgcacgcgc agttgcccgc atcgtcccgc tattacggga tcggcgggcc gcgcatgacc gccgtggatt tcgacgcgca ctggccgatg gaaaagctcg cggtgcgcgg ctacgtcgag gcgctcaagc acattcccga gatcctgcgc attcgcggcg aactcaagcg ccagttgctc gctgaaccgc ccgacgcgtt catcggcatc gacgcgcccg atttcaactt cggcctcgag caggcgctgc gcggcgccgg cattccgacg gtccatttcg tctgcccgtc gatctgggca tggcgcggcg gccggatcaa gaagatcgtc aaggccgtcg accacatgct gtgcctgttc ccgttcgagc ccgaactgct cgagaaggcc ggcgtcgcgg cgacgttcgt cggccatccg ctcgccgacg agattccgct cgaacccgac atgcacggcg cgcgaatcgc gctcggcctg ccggatagcg gccccgtgat cgcggtgctg ccgggcagcc gccgctcgga gatcgagctg atcggcccga cgttcttcga cgcgatggcg ttgatgcagc agcgcgagcc cggcgtgcgc ttcgtcgtgc cggccgcgac gcccgcgctg cgcgagttgc tgcagccgct cgtcgacgcg cacccgttgc tgtcggtcac gctgaccgaa aggncgcgcg caggtcgcga tgacngcggc cgatgcgatc ctcgtgaana gcggnnccgt gacnctcgaa gcngcgctgc tcnagaaacc gatggtgatc ncgtanagnc gtnnncacgg ggnanatcat gnnncnncag ggntatctgc cgnacgtnnn annnnnnaac atnntnnngg gnnntttcgt cnngcnnnan ctgnngcnnc nttcnnnncn cnnngnnnnn nncnannnnn nnntnnnann cnnannnnnn nnnnnnnnnn nnnnnnttnn nnnnnnnnnn annnnnnnnn nnnnnnngnn nnnannnnnn g
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAFQPTPLR VALVAGEPSG DLLGASLLGG LHAQLPASSR YYGIGGPRMT AVDFDAHWPM EKLAVRGYVE ALKHIPEILR IRGELKRQLL AEPPDAFIGI DAPDFNFGLE QALRGAGIPT VHFVCPSIWA WRGGRIKKIV KAVDHMLCLF PFEPELLEKA GVAATFVGHP LADEIPLEPD MHGARIALGL PDSGPVIAVL PGSRRSEIEL IGPTFFDAMA LMQQREPGVR FVVPAATPAL RELLQPLVDA HPLLSVTLTE GRAQVAMTAA DAILVKSGTV TLEAALLKKP MVISYKVPWL TGQIMRRQGY LPYVGLPNIL AGRFVVPELL QHFATPEALA DATLTQLRDD ANRRTLTGIF TDMHLALRQN TAQRAAEAVA RVIDSRKPH
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgttccaa cccacaccgc tgcgcgtcgc gctcgtcgcg ggcgaaccgt cgggcgacct gctcggcgcg tcgctcctcg gcgggctgca cgcgcagttg cccgcatcgt cccgctatta cgggatcggc gggccgcgca tgaccgccgt ggatttcgac gcgcactggc cgatggaaaa gctcgcggtg cgcggctacg tcgaggcgct caagcacatt cccgagatcc tgcgcattcg cggcgaactc aagcgccagt tgctcgctga accgcccgac gcgttcatcg gcatcgacgc gcccgatttc aacttcggcc tcgagcaggc gctgcgcggc gccggcattc cgacggtcca tttcgtctgc ccgtcgatct gggcatggcg cggcggccgg atcaagaaga tcgtcaaggc cgtcgaccac atgctgtgcc tgttcccgtt cgagcccgaa ctgctcgaga aggccggcgt cgcggcgacg ttcgtcggcc atccgctcgc cgacgagatt ccgctcgaac ccgacatgca cggcgcgcga atcgcgctcg gcctgccgga tagcggcccc gtgatcgcgg tgctgccggg cagccgccgc tcggagatcg agctgatcgg cccgacgttc ttcgacgcga tggcgttgat gcagcagcgc gagcccggcg tgcgcttcgt cgtgccggcc gcgacgcccg cgctgcgcga gttgctgcag ccgctcgtcg acgcgcaccc gttgctgtcg gtcacgctga ccgaaggccg cgcgcaggtc gcgatgacgg cggccgatgc gatcctcgtg aagagcggca ccgtgacgct cgaagcggcg ctgctcaaga aaccgatggt gatctcgtac aaggtgccgt ggctcacggg gcagatcatg cgccggcagg gctatctgcc gtacgtcgga ttgccgaaca tcctcgcggg ccgtttcgtc gtgcctgagc tgctgcagca tttcgcgacg cccgaggcgc tcgccgacgc gacgctcacg caactgcgcg acgatgcgaa ccgccgcacg ctcactggta ttttcaccga catgcatctc gcgttgcgcc agaacaccgc gcagcgcgcg gccgaggccg tcgcgcgcgt gatcgacagc aggaagccgc actaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa