ButhA.17953.a

Electron transfer flavoprotein, beta subunit (BTH_I1653)

CENTER ID: ButhA.17953.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.17953.a.A1.GE32977 full length 1 249
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.17953.a.A1.PS01222 full length 1 249

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.4439
RefSeq: YP_442190.1
UniProt: Q2SY09

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKILVPVKRV VDYNVKVRVK SDGTGVDIAN VKMSMNPFDE IAVEEAVRLK EAGVATEVIA VSVGVAQAQE TLRTALAIGA DRAILVESND EVQPLSVAKI LKALVDKEQP QLVILGKQAI DDDSNQTGQM LAALANLPQA TFASKVTVAD GKATVAREVD GGAETLSLTL PAVVTTDLRL NEPRYVTLPN IMKAKKKPLE TVKPEDLGVD VAPRLKTLKV VEPPKRAAGV KVADVKTLVE KLKTEAKVL
NT Sequence
atgaaaatcc tggtgccagt gaaaagagtg gtcgattaca acgtgaaggt ccgcgtgaag tcggatggca cgggcgtcga tatcgcgaac gtgaagatgt cgatgaaccc gttcgacgag atcgccgttg aggaagcggt gcgcctgaag gaagcgggcg tggcgaccga ggtgatcgcg gtgtcggtgg gcgtcgcgca ggctcaggaa acgcttcgca cggcgctcgc gatcggcgcg gaccgcgcga ttctcgtcga atcgaatgac gaggtgcagc cgctttccgt cgcgaagatc ctgaaggcgc tggtcgacaa ggagcagccg cagctcgtga ttctcggcaa gcaggcgatc gacgacgact cgaaccagac gggccagatg ctcgccgcgc tcgcgaatct gccgcaagcg acgttcgcgt cgaaggtgac ggttgcggac ggcaaggcga ccgtcgcccg tgaagtggac ggcggcgcgg aaacgctgtc gctgacgctg ccggccgtcg tgacgacgga cttgcgcctg aacgagccgc gctacgtgac gctgccgaac atcatgaagg cgaagaagaa gccgttggag acggtgaagc cggaggacct gggcgtggac gtcgcgccgc gtctgaagac gctgaaggtg gtcgagccgc ccaagcgcgc ggcgggcgtg aaggtcgcgg acgtgaagac gctggtcgag aagctgaaga ccgaagccaa ggtgctgtaa
Details for ButhA.17953.a.A1.GE32977
HARVESTED ON: 8/16/2011
SEQUENCED ON: 8/22/2011
EXPECTED MW: 28kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKILVPVKR VVDYNVKVRV KSDGTGVDIA NVKMSMNPFD EIAVEEAVRL KEAGVATEVI AVSVGVAQAQ ETLRTALAIG ADRAILVESN DEVQPLSVAK ILKALVDKEQ PQLVILGKQA IDDDSNQTGQ MLAALANLPQ ATFASKVTVA DGKATVAREV DGGAETLSLT LPAVVTTDLR LNEPRYVTLP NIMKAKKKPL ETVKPEDLGV DVAPRLKTLK VVEPPKRAAG VKVADVKTLV EKLKTEAKVL
Validated NT Sequence
ttattacagc accttggctt cggtcttcag cttctcgacc agcgtcttca cgtccgcgac cttcacgccc gccgcgcgct tgggcggctc gaccaccttc agcgtcttca gacgcggcgc gacgtccacg cccaggtcct ccggcttcac cgtctccaac ggcttcttct tcgccttcat gatgttcggc agcgtcacgt agcgcggctc gttcaggcgc aagtccgtcg tcacgacggc cggcagcgtc agcgacagcg tttccgcgcc gccgtccact tcacgggcga cggtcgcctt gccgtccgca accgtcacct tcgacgcgaa cgtcgcttgc ggcagattcg cgagcgcggc gagcatctgg cccgtctggt tcgagtcgtc gtcgatcgcc tgcttgccga gaatcacgag ctgcggctgc tccttgtcga ccagcgcctt caggatcttc gcgacggaaa gcggctgcac ctcgtcattc gattcgacga gaatcgcgcg gtccgcgccg atcgcgagcg ccgtgcgaag cgtttcctga gcctgcgcga cgcccaccga caccgcgatc acctcggtcg ccacgcccgc ttccttcagg cgcaccgctt cctcaacggc gatctcgtcg aacgggttca tcgacatctt cacgttcgcg atatcgacgc ccgtgccatc cgacttcacg cggaccttca cgttgtaatc gaccactctt ttcactggca ccaggatttt catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKILVPVKR VVDYNVKVRV KSDGTGVDIA NVKMSMNPFD EIAVEEAVRL KEAGVATEVI AVSVGVAQAQ ETLRTALAIG ADRAILVESN DEVQPLSVAK ILKALVDKEQ PQLVILGKQA IDDDSNQTGQ MLAALANLPQ ATFASKVTVA DGKATVAREV DGGAETLSLT LPAVVTTDLR LNEPRYVTLP NIMKAKKKPL ETVKPEDLGV DVAPRLKTLK VVEPPKRAAG VKVADVKTLV EKLKTEAKVL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaaatcctg gtgccagtga aaagagtggt cgattacaac gtgaaggtcc gcgtgaagtc ggatggcacg ggcgtcgata tcgcgaacgt gaagatgtcg atgaacccgt tcgacgagat cgccgttgag gaagcggtgc gcctgaagga agcgggcgtg gcgaccgagg tgatcgcggt gtcggtgggc gtcgcgcagg ctcaggaaac gcttcgcacg gcgctcgcga tcggcgcgga ccgcgcgatt ctcgtcgaat cgaatgacga ggtgcagccg ctttccgtcg cgaagatcct gaaggcgctg gtcgacaagg agcagccgca gctcgtgatt ctcggcaagc aggcgatcga cgacgactcg aaccagacgg gccagatgct cgccgcgctc gcgaatctgc cgcaagcgac gttcgcgtcg aaggtgacgg ttgcggacgg caaggcgacc gtcgcccgtg aagtggacgg cggcgcggaa acgctgtcgc tgacgctgcc ggccgtcgtg acgacggact tgcgcctgaa cgagccgcgc tacgtgacgc tgccgaacat catgaaggcg aagaagaagc cgttggagac ggtgaagccg gaggacctgg gcgtggacgt cgcgccgcgt ctgaagacgc tgaaggtggt cgagccgccc aagcgcgcgg cgggcgtgaa ggtcgcggac gtgaagacgc tggtcgagaa gctgaagacc gaagccaagg tgctgtaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for ButhA.17953.a.A1.PS01222
PURIFICATION DATe: 9/29/2011
CONCENTRATION: 37.18mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKILVPVKR VVDYNVKVRV KSDGTGVDIA NVKMSMNPFD EIAVEEAVRL KEAGVATEVI AVSVGVAQAQ ETLRTALAIG ADRAILVESN DEVQPLSVAK ILKALVDKEQ PQLVILGKQA IDDDSNQTGQ MLAALANLPQ ATFASKVTVA DGKATVAREV DGGAETLSLT LPAVVTTDLR LNEPRYVTLP NIMKAKKKPL ETVKPEDLGV DVAPRLKTLK VVEPPKRAAG VKVADVKTLV EKLKTEAKVL
Validated NT Sequence
ttattacagc accttggctt cggtcttcag cttctcgacc agcgtcttca cgtccgcgac cttcacgccc gccgcgcgct tgggcggctc gaccaccttc agcgtcttca gacgcggcgc gacgtccacg cccaggtcct ccggcttcac cgtctccaac ggcttcttct tcgccttcat gatgttcggc agcgtcacgt agcgcggctc gttcaggcgc aagtccgtcg tcacgacggc cggcagcgtc agcgacagcg tttccgcgcc gccgtccact tcacgggcga cggtcgcctt gccgtccgca accgtcacct tcgacgcgaa cgtcgcttgc ggcagattcg cgagcgcggc gagcatctgg cccgtctggt tcgagtcgtc gtcgatcgcc tgcttgccga gaatcacgag ctgcggctgc tccttgtcga ccagcgcctt caggatcttc gcgacggaaa gcggctgcac ctcgtcattc gattcgacga gaatcgcgcg gtccgcgccg atcgcgagcg ccgtgcgaag cgtttcctga gcctgcgcga cgcccaccga caccgcgatc acctcggtcg ccacgcccgc ttccttcagg cgcaccgctt cctcaacggc gatctcgtcg aacgggttca tcgacatctt cacgttcgcg atatcgacgc ccgtgccatc cgacttcacg cggaccttca cgttgtaatc gaccactctt ttcactggca ccaggatttt catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKILVPVKR VVDYNVKVRV KSDGTGVDIA NVKMSMNPFD EIAVEEAVRL KEAGVATEVI AVSVGVAQAQ ETLRTALAIG ADRAILVESN DEVQPLSVAK ILKALVDKEQ PQLVILGKQA IDDDSNQTGQ MLAALANLPQ ATFASKVTVA DGKATVAREV DGGAETLSLT LPAVVTTDLR LNEPRYVTLP NIMKAKKKPL ETVKPEDLGV DVAPRLKTLK VVEPPKRAAG VKVADVKTLV EKLKTEAKVL
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAAAATCCTG GTGCCAGTGA AAAGAGTGGT CGATTACAAC GTGAAGGTCC GCGTGAAGTC GGATGGCACG GGCGTCGATA TCGCGAACGT GAAGATGTCG ATGAACCCGT TCGACGAGAT CGCCGTTGAG GAAGCGGTGC GCCTGAAGGA AGCGGGCGTG GCGACCGAGG TGATCGCGGT GTCGGTGGGC GTCGCGCAGG CTCAGGAAAC GCTTCGCACG GCGCTCGCGA TCGGCGCGGA CCGCGCGATT CTCGTCGAAT CGAATGACGA GGTGCAGCCG CTTTCCGTCG CGAAGATCCT GAAGGCGCTG GTCGACAAGG AGCAGCCGCA GCTCGTGATT CTCGGCAAGC AGGCGATCGA CGACGACTCG AACCAGACGG GCCAGATGCT CGCCGCGCTC GCGAATCTGC CGCAAGCGAC GTTCGCGTCG AAGGTGACGG TTGCGGACGG CAAGGCGACC GTCGCCCGTG AAGTGGACGG CGGCGCGGAA ACGCTGTCGC TGACGCTGCC GGCCGTCGTG ACGACGGACT TGCGCCTGAA CGAGCCGCGC TACGTGACGC TGCCGAACAT CATGAAGGCG AAGAAGAAGC CGTTGGAGAC GGTGAAGCCG GAGGACCTGG GCGTGGACGT CGCGCCGCGT CTGAAGACGC TGAAGGTGGT CGAGCCGCCC AAGCGCGCGG CGGGCGTGAA GGTCGCGGAC GTGAAGACGC TGGTCGAGAA GCTGAAGACC GAAGCCAAGG TGCTGTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA