SSGCID: Target Page

ButhA.17951.a

Aspartokinase (EC 2.7.2.4) (BTH_I1945)

CENTER ID:	ButhA.17951.a
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.17951.a.A1.GE32949	full length		1	416

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.4752
RefSeq:	YP_442475.1
UniProt:	Q2SX74

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MALIVHKYGG TSMGSVERIK NVAKRVAKWH QAGHQMVVVP SAMSGETNRL LGLAKEISSQ PSPRELDMIA STGEQVSVGL LSIAMQEIGV EAVSYAGWQV PIKTDSAYTK ARIHSIDDER VRRDLDAGKV VIITGFQGVD PDGHITTLGR GGSDTSAVAV AAALEADECL IYTDVDGVYT TDPRVVEEAR RLDSVTFEEM LEMASLGSKV LQIRSVEFAG KYRVKTRVLS SLTDPQIPLD EEMRSGTLIT FEEDETMEKA VISGIAFQRD EARIAVMGVP DKPGIAYQIL GPVADANIDV DMIIQNQSVE GKTDFTFTVG RGDYQKAMDI LTNQVKGHVN AEQVLGDPKV SKVSVVGVGM RSHVGVASTM FRTLSEEGIN IQMISTSEIK ISVLIDEKYM ELAVRALHKA FELDQA

NT Sequence

atggcactca tcgtacataa atacggcggc acttcgatgg gctcggtcga gcgcatcaag aacgtcgcga aacgcgtcgc aaaatggcat caggccgggc accagatggt ggtcgtgccg tccgcgatgt ccggcgaaac gaaccgcctg ctcggtctcg cgaaagagat ctcgagccag ccgagcccgc gcgagctcga catgatcgcg tcgacgggcg agcaggtcag cgtcggcctc ttgtcgatcg caatgcagga aatcggcgtc gaggcggtga gctacgccgg ctggcaggtg ccgatcaaga ccgacagcgc gtacacgaag gcgcgcattc attcgatcga cgacgagcgc gtgcgccgcg atctcgacgc gggcaaggtc gtcatcatca cgggttttca gggcgtcgat ccggacggcc acatcacgac gcttggccgc ggcggctcgg acacgtcggc ggtcgcggtc gcggcggcgc tcgaggcgga cgaatgcctg atctacacgg acgtcgacgg cgtgtacacg accgatccgc gcgtcgtcga agaggcgcgc cggctcgata gcgtgacgtt cgaggaaatg ctggaaatgg cgagcctcgg ctcgaaggtg ttgcagatcc gctcggtcga gttcgcgggc aagtaccgcg tgaagacgcg cgtgctgtcg agcctcaccg atccgcagat tccgctcgac gaagagatgc gctcgggtac cctgattact tttgaagaag acgagaccat ggaaaaggca gtcatttccg gcattgcgtt ccagcgcgac gaagcgcgca ttgcggtgat gggcgtgccc gacaagccgg gcatcgcata tcagatcctc ggccctgtcg cggatgcgaa catcgacgtc gacatgatca tccagaacca gagcgtcgaa gggaaaaccg acttcacgtt tacggtcggc cgcggcgatt accagaaggc gatggacatc ctcacgaacc aggtgaaggg ccacgtgaac gccgagcagg tgctgggcga cccgaaggtg tcgaaggtgt cggtggtcgg cgtcgggatg cgttcgcacg tcggcgtcgc gagcacgatg ttccgcacgc tgtcggagga gggcatcaac atccagatga tctcgacgtc cgaaatcaag atttcggtgc tgatcgacga gaaatacatg gaattggcgg tccgcgcact gcacaaggca ttcgaactcg atcaggcgtg a

Details for ButhA.17951.a.A1.GE32949

HARVESTED ON:	7/12/2011
SEQUENCED ON:	7/28/2011
EXPECTED MW:	47kDa
OBSERVED MW:	46kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	90
PERCENT COVERAGE:	73

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMALIVHKYG GTSMGSVERI KNVAKRVAKW HQAGHQMVVV PSAMSGETNR LLGLAKEISS QPSPRELDMI ASTGEQVSVG LLSIAMQEIG VEAVSYAGWQ VPIKTDSAYT KARIHSIDDE RVRRDLDAGK VVIITGFQGV DPDGHITTLG RGGSDTSAVA VAAALEADEC LIYTDVDGVY TTDPRVVEEA RRLDSVTFEE MLEMASLGSK VLQIRSVEFA GKYRVKTRVL SSLTDPQIPL DEEMRSGTLI TFEEDETMEK AVIXRHCVPA RRXAHCGDGR ARXAGHRXSX PRPXRRXSXS T

Validated NT Sequence

ttttntttac tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggcact catcgtacat aaatacggcg gcacttcgat gggctcggtc gagcgcatca agaacgtcgc gaaacgcgtc gcaaaatggc atcaggccgg gcaccagatg gtggtcgtgc cgtccgcgat gtccggcgaa acgaaccgcc tgctcggtct cgcgaaagag atctcgagcc agccgagccc gcgcgagctc gacatgatcg cgtcgacggg cgagcaggtc agcgtcggcc tcttgtcgat cgcaatgcag gaaatcggcg tcgaggcggt gagctacgcc ggctggcagg tgccgatcaa gaccgacagc gcgtacacga aggcgcgcat tcattcgatc gacgacgagc gcgtgcgccg cgatctcgac gcgggcaagg tcgtcatcat cacgggtttt cagggcgtcg atccggacgg ccacatcacg acgcttggcc gcggcggctc ggacacgtcg gcggtcgcgg tcgcggcggc gctcgaggcg gacgaatgcc tgatctacac ggacgtcgac ggcgtgtaca cgaccgatcc gcgcgtcgtc gaagaggcgc gccggctcga tagcgtgacg ttcgaggaaa tgctggaaat ggcgagcctc ggctcgaagg tgttgcagat ccgctcggtc gagttcgcgg gcaagtaccg cgtgaagacg cgcgtgctgt cgagcctcac cgatccgcag attccgctcg acgaagagat gcgctcgggt accctgatta cttttgaaga agacgagacc atggaaaagg cagtcatttn ccggcattgc gttccagcgc gacgaancgc gcattgcggt gatgggcgtg cccganaagc cgggcatcgn atntcanatc ctcggccctg ncggcgaana tcgncgtcga cntgatcatc cngaancaga ncgtcnaagg naaanngact tcncntttnn ngncngnngc ngnnanncnn nnnnngatgg ncatnntcnc nnnnnnnnan ggnncnnnnn nncnannngn nctggncnan ccnngnnnnc nnnnnnnnng nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc cgnnnnnnnn nnnnnnnnnn nnc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcactcatc gtacataaat acggcggcac ttcgatgggc tcggtcgagc gcatcaagaa cgtcgcgaaa cgcgtcgcaa aatggcatca ggccgggcac cagatggtgg tcgtgccgtc cgcgatgtcc ggcgaaacga accgcctgct cggtctcgcg aaagagatct cgagccagcc gagcccgcgc gagctcgaca tgatcgcgtc gacgggcgag caggtcagcg tcggcctctt gtcgatcgca atgcaggaaa tcggcgtcga ggcggtgagc tacgccggct ggcaggtgcc gatcaagacc gacagcgcgt acacgaaggc gcgcattcat tcgatcgacg acgagcgcgt gcgccgcgat ctcgacgcgg gcaaggtcgt catcatcacg ggttttcagg gcgtcgatcc ggacggccac atcacgacgc ttggccgcgg cggctcggac acgtcggcgg tcgcggtcgc ggcggcgctc gaggcggacg aatgcctgat ctacacggac gtcgacggcg tgtacacgac cgatccgcgc gtcgtcgaag aggcgcgccg gctcgatagc gtgacgttcg aggaaatgct ggaaatggcg agcctcggct cgaaggtgtt gcagatccgc tcggtcgagt tcgcgggcaa gtaccgcgtg aagacgcgcg tgctgtcgag cctcaccgat ccgcagattc cgctcgacga agagatgcgc tcgggtaccc tgattacttt tgaagaagac gagaccatgg aaaaggcagt catttccggc attgcgttcc agcgcgacga agcgcgcatt gcggtgatgg gcgtgcccga caagccgggc atcgcatatc agatcctcgg ccctgtcgcg gatgcgaaca tcgacgtcga catgatcatc cagaaccaga gcgtcgaagg gaaaaccgac ttcacgttta cggtcggccg cggcgattac cagaaggcga tggacatcct cacgaaccag gtgaagggcc acgtgaacgc cgagcaggtg ctgggcgacc cgaaggtgtc gaaggtgtcg gtggtcggcg tcgggatgcg ttcgcacgtc ggcgtcgcga gcacgatgtt ccgcacgctg tcggaggagg gcatcaacat ccagatgatc tcgacgtccg aaatcaagat ttcggtgctg atcgacgaga aatacatgga attggcggtc cgcgcactgc acaaggcatt cgaactcgat caggcgtaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa