ButhA.17950.a

Ribosome maturation factor rimM (BTH_I1662) (rimM)

CENTER ID: ButhA.17950.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.17950.a.A1.GE32948 full length 1 229
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.17950.a.A1.PW33552 full length 1 229

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.4448
RefSeq: YP_442199.1
UniProt: Q2SY00

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAGHDSGSAK RGRSPSFGVF VRKPVERAPT KGAGDGAADS EAIRIDAAQP WPDDAVEVGA VVDAYGLKGW VKLVAHAGAG RGGDALLKAR DWWLQKGGER KFARVTQAKL HSDAVVAHPA GSVDRDTALA LRGFRVFVRR GDFPALAADE FYWVDLIGLD VVNEAGVALG KVADMIDNGV HSIMRVEYPA TGKDGRPTTG ERLIPFVGVY VKAVEQAAGR IVVDWEADY
NT Sequence
atggctggtc acgattccgg tagcgcgaag cgcgggcggt cgccgtcgtt cggcgtattc gtccgcaagc cggtcgagcg cgcgcccacg aaaggggcgg gcgacggcgc ggctgattcg gaagcaattc gcatcgatgc ggcgcagccg tggccggacg atgcggtcga ggtcggcgcg gtggtcgatg cgtacggcct caagggctgg gtcaagctcg tggcccatgc cggggcgggg cggggcggcg atgcgctgct caaggctcgc gactggtggc tgcaaaaggg gggcgagcgg aagttcgcgc gcgtcacgca ggcgaagctg cacagcgacg cggtcgtcgc gcatccggcc ggctctgtcg atcgcgatac ggcgcttgcg ctgcgcggct ttcgcgtatt cgtgcgccgc ggcgatttcc cggcgctcgc cgccgacgag ttctactggg tcgatctgat tggcctcgac gtggtcaacg aggctggcgt cgcgctcggc aaggttgccg atatgattga caacggcgtg cattcgatca tgcgcgtcga atatccggcg accggcaagg atggccggcc gacgaccggc gagcggctga ttccgttcgt cggcgtgtac gtgaaggcgg tcgagcaggc ggcgggacgc atcgtcgtcg actgggaagc cgattactga
Details for ButhA.17950.a.A1.GE32948
HARVESTED ON: 7/12/2011
SEQUENCED ON: 7/28/2011
EXPECTED MW: 26kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAGHDSGSA KRGRSPSFGV FVRKPVERAP TKGAGDGAAD SEAIRIDAAQ PWPDDAVEVG AVVDAYGLKG WVKLVAHAGA GRGGDALLKA RDWWLQKGGE RKFARVTQAK LHSDAVVAHP AGSVDRDTAL ALRGFRVFVR RGDFPALAAD EFYWVDLIGL DVVNEAGVAL GKVADMIDNG VHSIMRVEYP ATGKDGRPTT GERLIPFVGV YVKAVEQAAG RIVVDWEA
Validated NT Sequence
cggcttccca gtcgacgacg atgcgtcccg ccgcctgctc gaccgccttc acgtacacgc cgacgaacgg aatcagccgc tcgccggtcg tcggccggcc atccttgccg gtcgccggat attcgacgcg catgatcgaa tgcacgccgt tgtcaatcat atcggcaacc ttgccgagcg cgacgccagc ctcgttgacc acgtcgaggc caatcagatc gacccagtag aactcgtcgg cggcgagcgc cgggaaatcg ccgcggcgca cgaatacgcg aaagccgcgc agcgcaagcg ccgtatcgcg atcgacagag ccggccggat gcgcgacgac cgcgtcgctg tgcagcttcg cctgcgtgac gcgcgcgaac ttccgctcgc cccccttttg cagccaccag tcgcgagcct tgagcagcgc atcgccgccc cgccccgccc cggcatgggc cacgagcttg acccagccct tgaggccgta cgcatcgacc accgcgccga cctcgaccgc atcgtccggc cacggctgcg ccgcatcgat gcgaattgct tccgaatcag ccgcgccgtc gcccgcccct ttcgtgggcg cgcgctcgac cggcttgcgg acgaatacgc cgaacgacgg cgaccgcccg cgcttcgcgc taccggaatc gtgaccagcc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga gccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAGHDSGSA KRGRSPSFGV FVRKPVERAP TKGAGDGAAD SEAIRIDAAQ PWPDDAVEVG AVVDAYGLKG WVKLVAHAGA GRGGDALLKA RDWWLQKGGE RKFARVTQAK LHSDAVVAHP AGSVDRDTAL ALRGFRVFVR RGDFPALAAD EFYWVDLIGL DVVNEAGVAL GKVADMIDNG VHSIMRVEYP ATGKDGRPTT GERLIPFVGV YVKAVEQAAG RIVVDWEADY
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggctggtcac gattccggta gcgcgaagcg cgggcggtcg ccgtcgttcg gcgtattcgt ccgcaagccg gtcgagcgcg cgcccacgaa aggggcgggc gacggcgcgg ctgattcgga agcaattcgc atcgatgcgg cgcagccgtg gccggacgat gcggtcgagg tcggcgcggt ggtcgatgcg tacggcctca agggctgggt caagctcgtg gcccatgccg gggcggggcg gggcggcgat gcgctgctca aggctcgcga ctggtggctg caaaaggggg gcgagcggaa gttcgcgcgc gtcacgcagg cgaagctgca cagcgacgcg gtcgtcgcgc atccggccgg ctctgtcgat cgcgatacgg cgcttgcgct gcgcggcttt cgcgtattcg tgcgccgcgg cgatttcccg gcgctcgccg ccgacgagtt ctactgggtc gatctgattg gcctcgacgt ggtcaacgag gctggcgtcg cgctcggcaa ggttgccgat atgattgaca acggcgtgca ttcgatcatg cgcgtcgaat atccggcgac cggcaaggat ggccggccga cgaccggcga gcggctgatt ccgttcgtcg gcgtgtacgt gaaggcggtc gagcaggcgg cgggacgcat cgtcgtcgac tgggaagccg attactaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for ButhA.17950.a.A1.PW33552
PURIFICATION DATe: 9/30/2011
CONCENTRATION: 25.17mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAGHDSGSA KRGRSPSFGV FVRKPVERAP TKGAGDGAAD SEAIRIDAAQ PWPDDAVEVG AVVDAYGLKG WVKLVAHAGA GRGGDALLKA RDWWLQKGGE RKFARVTQAK LHSDAVVAHP AGSVDRDTAL ALRGFRVFVR RGDFPALAAD EFYWVDLIGL DVVNEAGVAL GKVADMIDNG VHSIMRVEYP ATGKDGRPTT GERLIPFVGV YVKAVEQAAG RIVVDWEA
Validated NT Sequence
cggcttccca gtcgacgacg atgcgtcccg ccgcctgctc gaccgccttc acgtacacgc cgacgaacgg aatcagccgc tcgccggtcg tcggccggcc atccttgccg gtcgccggat attcgacgcg catgatcgaa tgcacgccgt tgtcaatcat atcggcaacc ttgccgagcg cgacgccagc ctcgttgacc acgtcgaggc caatcagatc gacccagtag aactcgtcgg cggcgagcgc cgggaaatcg ccgcggcgca cgaatacgcg aaagccgcgc agcgcaagcg ccgtatcgcg atcgacagag ccggccggat gcgcgacgac cgcgtcgctg tgcagcttcg cctgcgtgac gcgcgcgaac ttccgctcgc cccccttttg cagccaccag tcgcgagcct tgagcagcgc atcgccgccc cgccccgccc cggcatgggc cacgagcttg acccagccct tgaggccgta cgcatcgacc accgcgccga cctcgaccgc atcgtccggc cacggctgcg ccgcatcgat gcgaattgct tccgaatcag ccgcgccgtc gcccgcccct ttcgtgggcg cgcgctcgac cggcttgcgg acgaatacgc cgaacgacgg cgaccgcccg cgcttcgcgc taccggaatc gtgaccagcc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga gccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAGHDSGSA KRGRSPSFGV FVRKPVERAP TKGAGDGAAD SEAIRIDAAQ PWPDDAVEVG AVVDAYGLKG WVKLVAHAGA GRGGDALLKA RDWWLQKGGE RKFARVTQAK LHSDAVVAHP AGSVDRDTAL ALRGFRVFVR RGDFPALAAD EFYWVDLIGL DVVNEAGVAL GKVADMIDNG VHSIMRVEYP ATGKDGRPTT GERLIPFVGV YVKAVEQAAG RIVVDWEADY
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCTGGTCAC GATTCCGGTA GCGCGAAGCG CGGGCGGTCG CCGTCGTTCG GCGTATTCGT CCGCAAGCCG GTCGAGCGCG CGCCCACGAA AGGGGCGGGC GACGGCGCGG CTGATTCGGA AGCAATTCGC ATCGATGCGG CGCAGCCGTG GCCGGACGAT GCGGTCGAGG TCGGCGCGGT GGTCGATGCG TACGGCCTCA AGGGCTGGGT CAAGCTCGTG GCCCATGCCG GGGCGGGGCG GGGCGGCGAT GCGCTGCTCA AGGCTCGCGA CTGGTGGCTG CAAAAGGGGG GCGAGCGGAA GTTCGCGCGC GTCACGCAGG CGAAGCTGCA CAGCGACGCG GTCGTCGCGC ATCCGGCCGG CTCTGTCGAT CGCGATACGG CGCTTGCGCT GCGCGGCTTT CGCGTATTCG TGCGCCGCGG CGATTTCCCG GCGCTCGCCG CCGACGAGTT CTACTGGGTC GATCTGATTG GCCTCGACGT GGTCAACGAG GCTGGCGTCG CGCTCGGCAA GGTTGCCGAT ATGATTGACA ACGGCGTGCA TTCGATCATG CGCGTCGAAT ATCCGGCGAC CGGCAAGGAT GGCCGGCCGA CGACCGGCGA GCGGCTGATT CCGTTCGTCG GCGTGTACGT GAAGGCGGTC GAGCAGGCGG CGGGACGCAT CGTCGTCGAC TGGGAAGCCG ATTACTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA