ButhA.17946.a

DNA ligase (EC 6.5.1.2) (Polydeoxyribonucleotide synthase [NAD+]) (BTH_I2022) (ligA)

CENTER ID: ButhA.17946.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.17946.a.A1.GE32944 full length 1 691
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.17946.a.A1.PS01204 full length 1 691

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.4831
RefSeq: YP_442546.1
UniProt: Q2SX03

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MARSPVEPSA GQPAKRAAWL RAELERANYA YYVLDQPDLP DAEYDRLFAE LQQIEAEHPD LVTPDSPTQR VGGEVASGFT PVVHDTPMLS LNNGFSDDDV VAFDKRVADG LDKPTDLAGT VTEPVEYACE LKFDGLAISL RYENGRFVQA STRGDGTTGE DVTENIRTVR SIPLTLKGKR VPRMLDVRGE VLMFRRDFAR LNERQRAAGQ REFANPRNAA AGSLRQLDSK ITASRPLSFF AYGIGVLDGV EMPDTHSSLL DWYETLGLPV NRERAVVRGA AGLLEFFHSV GARRESLPYD IDGVVYKVNR RDEQDRLGFV SRAPRFALAH KFPAQEALTK LVAIDVQVGR TGAITPVARL EPVFVGGATV TNATLHNEDE VRRKDIRIGD TVIVRRAGDV IPEVVSAVFD RRPADAREFV MPTECPECGS RVERLPDEAI ARCTGGLFCP AQRKQALWHF AQRRALDIDG LGEKIIDQLV EQNLVRTPAD LFNLGFSTLV ELDRFAEKSA QNLIDSLEKA KHTTLARFIY ALGIRHVGES TAKDLAKHFG SLDPIMDASI DELLEVNDVG PIVAESIHQF FAEAHNRTVI EQLRAKGKVT WPEGPPAPRA PQGVLAGKTV VLTGTLPTLT REAAKEMLEA AGAKVAGSVS KKTDYVVAGA DAGSKLAKAE ELGIPVLDEA GMHKLLEGHA R
NT Sequence
atggcccgat ccccagttga accgtccgcc ggccagccgg cgaagcgcgc cgcatggctg cgcgccgagc tcgagcgggc gaactacgca tattacgtgc tcgaccagcc ggacctgccc gacgccgagt acgaccgcct cttcgccgaa ctccagcaga tcgaggccga gcaccccgat ctcgtcacgc ccgattcgcc gacgcagcgc gtcggcggcg aggtcgcgag cggcttcacg ccggtcgttc acgatacgcc gatgctgtcg ctcaacaacg gtttttccga cgacgacgtc gtcgcgttcg acaagcgcgt cgccgacggc ctcgacaagc cgaccgacct cgccggcacc gtgaccgagc ctgtcgaata cgcgtgcgag ctgaagttcg acggcctggc gatctcgctg cgctacgaga acggccgctt cgtgcaggca tcgacgcgcg gcgacggcac gacgggcgaa gacgtcaccg agaacatccg cacggtccgc tcgattccgc tgacgctcaa gggcaagcgc gtgccgcgca tgctcgacgt gcgcggcgaa gtgctcatgt tcaggcgcga tttcgcgcgc ttgaacgagc gtcagcgcgc ggcgggccag cgcgaattcg ccaatccgcg caacgcggcg gcgggtagcc tgcggcagct cgattcgaag atcaccgcgt cgcggccgct ttcgtttttc gcctacggga tcggcgtgct cgacggcgtc gagatgcccg acacgcacag cagcctgctc gactggtacg agacgctcgg gctgccggtg aaccgcgagc gcgcggtcgt gcgcggcgcg gcgggcctgc tcgagttctt ccattcggtg ggcgcgcggc gcgagtcgct gccgtacgac atcgacggcg tcgtctacaa ggtgaatcgc cgcgacgagc aggatcggct cggcttcgtg tcgcgcgcgc cgcgcttcgc gctcgcgcac aagttccccg cgcaggaggc gctgacgaag ctcgtcgcga tcgacgtgca ggtcggccgc acgggcgcga tcacgccggt cgcgcgtctc gagcccgtgt tcgtcggcgg cgcgaccgtc acgaacgcga cgctgcacaa cgaggacgag gtgcgccgca aggacatccg gatcggcgac acggtcatcg tgcgccgcgc gggcgacgtg attcccgaag tggtgtcggc cgtgttcgac cggcgtcccg ccgacgcgcg cgagttcgtg atgccgaccg agtgcccgga atgcggttcg cgggtcgagc gattgcccga cgaggcgatc gcgcgctgca cgggcgggct cttctgcccc gcgcagcgca agcaggcgct ctggcacttc gcgcagcgcc gcgcgctcga catcgacggg ctcggcgaga agatcatcga tcagctcgtc gagcagaacc tcgtgcgcac gccggccgat ctgttcaatc tcggcttctc gacgctcgtc gagctcgatc gttttgcgga gaagtccgcg cagaacctga tcgactcgct cgaaaaggcg aagcacacga cgctcgcgcg cttcatttac gcgctcggca tccggcacgt cggcgaatcg acggcgaagg acctcgcgaa gcacttcggc tcgctcgatc cgatcatgga cgcgtcgatc gacgaattgc tcgaagtcaa tgacgtcggg ccgatcgtcg ccgaatcgat ccatcagttc ttcgccgagg cgcacaatcg cacggtgatc gagcaactgc gcgcgaaggg caaagtcacg tggcccgaag ggccgcccgc gccgcgcgcg ccgcagggcg tgctcgcggg caagaccgtc gtgctgacgg gcacgctgcc gacgctcacg cgggaagccg cgaaggagat gctcgaggcg gcgggcgcga aggtcgcggg ctcggtgtcg aagaagaccg attacgtcgt cgcgggcgcc gacgcgggca gcaagctcgc gaaagccgag gagctcggca ttccggtgct ggacgaagca ggcatgcaca aacttctgga gggccatgcg cgatga
Details for ButhA.17946.a.A1.GE32944
HARVESTED ON: 7/12/2011
SEQUENCED ON: 7/28/2011
EXPECTED MW: 77kDa
OBSERVED MW: 80kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 97
PERCENT COVERAGE: 43
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMARSPVEPS AGQPAKRAAW LRAELERANY AYYVLDQPDL PDAEYDRLFA ELQQIEAEHP DLVTPDSPTQ RVGGEVASGF TPVVHDTPML SLNNGFSDDD VVAFDKRVAD GLDKPTDLAG TVTEPVEYAC ELKFDGLAIS LRYENGRFVQ ASTRGDGTTG EDVTENIRTV RSIPLTLKGK RVPRMLDVRG EVLMFRRDFA RLNERQRAAG QREFANPRNA AAGSLRQLDS KITASRPLSF FAYXXGVLDG VXMPDTHSSL LDWYETLGLX VXRERAVVRG AAGLLXFXH
Validated NT Sequence
aatgnaagaa ctngagcagn cccgccgcgc cgcgcacgac cgcgcgctcg cgnttcaccg ncagcccgag cgtctcgtac cagtcgagca ggctgctgtg cgtgtcgggc atctngacgc cgtcgagcac gccganccng taggcgaaaa acgaaagcgg ccgcgacgcg gtgatcttcg aatcgagctg ccgcaggcta cccgccgccg cgttgcgcgg attggcgaat tcgcgctggc ccgccgcgcg ctgacgctcg ttcaagcgcg cgaaatcgcg cctgaacatg agcacttcgc cgcgcacgtc gagcatgcgc ggcacgcgct tgcccttgag cgtcagcgga atcgagcgga ccgtgcggat gttctcggtg acgtcttcgc ccgtcgtgcc gtcgccgcgc gtcgatgcct gcacgaagcg gccgttctcg tagcgcagcg agatcgccag gccgtcgaac ttcagctcgc acgcgtattc gacaggctcg gtcacggtgc cggcgaggtc ggtcggcttg tcgaggccgt cggcgacgcg cttgtcgaac gcgacgacgt cgtcgtcgga aaaaccgttg ttgagcgaca gcatcggcgt atcgtgaacg accggcgtga agccgctcgc gacctcgccg ccgacgcgct gcgtcggcga atcgggcgtg acgagatcgg ggtgctcggc ctcgatctgc tggagttcgg cgaagaggcg gtcgtactcg gcgtcgggca ggtccggctg gtcgagcacg taatatgcgt agttcgcccg ctcgagctcg gcgcgcagcc atgcggcgcg cttcgccggc tggccggcgg acggttcaac tggggatcgg gccatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMARSPVEPS AGQPAKRAAW LRAELERANY AYYVLDQPDL PDAEYDRLFA ELQQIEAEHP DLVTPDSPTQ RVGGEVASGF TPVVHDTPML SLNNGFSDDD VVAFDKRVAD GLDKPTDLAG TVTEPVEYAC ELKFDGLAIS LRYENGRFVQ ASTRGDGTTG EDVTENIRTV RSIPLTLKGK RVPRMLDVRG EVLMFRRDFA RLNERQRAAG QREFANPRNA AAGSLRQLDS KITASRPLSF FAYGIGVLDG VEMPDTHSSL LDWYETLGLP VNRERAVVRG AAGLLEFFHS VGARRESLPY DIDGVVYKVN RRDEQDRLGF VSRAPRFALA HKFPAQEALT KLVAIDVQVG RTGAITPVAR LEPVFVGGAT VTNATLHNED EVRRKDIRIG DTVIVRRAGD VIPEVVSAVF DRRPADAREF VMPTECPECG SRVERLPDEA IARCTGGLFC PAQRKQALWH FAQRRALDID GLGEKIIDQL VEQNLVRTPA DLFNLGFSTL VELDRFAEKS AQNLIDSLEK AKHTTLARFI YALGIRHVGE STAKDLAKHF GSLDPIMDAS IDELLEVNDV GPIVAESIHQ FFAEAHNRTV IEQLRAKGKV TWPEGPPAPR APQGVLAGKT VVLTGTLPTL TREAAKEMLE AAGAKVAGSV SKKTDYVVAG ADAGSKLAKA EELGIPVLDE AGMHKLLEGH AR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcccgatcc ccagttgaac cgtccgccgg ccagccggcg aagcgcgccg catggctgcg cgccgagctc gagcgggcga actacgcata ttacgtgctc gaccagccgg acctgcccga cgccgagtac gaccgcctct tcgccgaact ccagcagatc gaggccgagc accccgatct cgtcacgccc gattcgccga cgcagcgcgt cggcggcgag gtcgcgagcg gcttcacgcc ggtcgttcac gatacgccga tgctgtcgct caacaacggt ttttccgacg acgacgtcgt cgcgttcgac aagcgcgtcg ccgacggcct cgacaagccg accgacctcg ccggcaccgt gaccgagcct gtcgaatacg cgtgcgagct gaagttcgac ggcctggcga tctcgctgcg ctacgagaac ggccgcttcg tgcaggcatc gacgcgcggc gacggcacga cgggcgaaga cgtcaccgag aacatccgca cggtccgctc gattccgctg acgctcaagg gcaagcgcgt gccgcgcatg ctcgacgtgc gcggcgaagt gctcatgttc aggcgcgatt tcgcgcgctt gaacgagcgt cagcgcgcgg cgggccagcg cgaattcgcc aatccgcgca acgcggcggc gggtagcctg cggcagctcg attcgaagat caccgcgtcg cggccgcttt cgtttttcgc ctacgggatc ggcgtgctcg acggcgtcga gatgcccgac acgcacagca gcctgctcga ctggtacgag acgctcgggc tgccggtgaa ccgcgagcgc gcggtcgtgc gcggcgcggc gggcctgctc gagttcttcc attcggtggg cgcgcggcgc gagtcgctgc cgtacgacat cgacggcgtc gtctacaagg tgaatcgccg cgacgagcag gatcggctcg gcttcgtgtc gcgcgcgccg cgcttcgcgc tcgcgcacaa gttccccgcg caggaggcgc tgacgaagct cgtcgcgatc gacgtgcagg tcggccgcac gggcgcgatc acgccggtcg cgcgtctcga gcccgtgttc gtcggcggcg cgaccgtcac gaacgcgacg ctgcacaacg aggacgaggt gcgccgcaag gacatccgga tcggcgacac ggtcatcgtg cgccgcgcgg gcgacgtgat tcccgaagtg gtgtcggccg tgttcgaccg gcgtcccgcc gacgcgcgcg agttcgtgat gccgaccgag tgcccggaat gcggttcgcg ggtcgagcga ttgcccgacg aggcgatcgc gcgctgcacg ggcgggctct tctgccccgc gcagcgcaag caggcgctct ggcacttcgc gcagcgccgc gcgctcgaca tcgacgggct cggcgagaag atcatcgatc agctcgtcga gcagaacctc gtgcgcacgc cggccgatct gttcaatctc ggcttctcga cgctcgtcga gctcgatcgt tttgcggaga agtccgcgca gaacctgatc gactcgctcg aaaaggcgaa gcacacgacg ctcgcgcgct tcatttacgc gctcggcatc cggcacgtcg gcgaatcgac ggcgaaggac ctcgcgaagc acttcggctc gctcgatccg atcatggacg cgtcgatcga cgaattgctc gaagtcaatg acgtcgggcc gatcgtcgcc gaatcgatcc atcagttctt cgccgaggcg cacaatcgca cggtgatcga gcaactgcgc gcgaagggca aagtcacgtg gcccgaaggg ccgcccgcgc cgcgcgcgcc gcagggcgtg ctcgcgggca agaccgtcgt gctgacgggc acgctgccga cgctcacgcg ggaagccgcg aaggagatgc tcgaggcggc gggcgcgaag gtcgcgggct cggtgtcgaa gaagaccgat tacgtcgtcg cgggcgccga cgcgggcagc aagctcgcga aagccgagga gctcggcatt ccggtgctgg acgaagcagg catgcacaaa cttctggagg gccatgcgcg ataaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for ButhA.17946.a.A1.PS01204
PURIFICATION DATe: 9/1/2011
CONCENTRATION: 36.2mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 200µl
PERCENT IDENTITY: 97
PERCENT COVERAGE: 43
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMARSPVEPS AGQPAKRAAW LRAELERANY AYYVLDQPDL PDAEYDRLFA ELQQIEAEHP DLVTPDSPTQ RVGGEVASGF TPVVHDTPML SLNNGFSDDD VVAFDKRVAD GLDKPTDLAG TVTEPVEYAC ELKFDGLAIS LRYENGRFVQ ASTRGDGTTG EDVTENIRTV RSIPLTLKGK RVPRMLDVRG EVLMFRRDFA RLNERQRAAG QREFANPRNA AAGSLRQLDS KITASRPLSF FAYXXGVLDG VXMPDTHSSL LDWYETLGLX VXRERAVVRG AAGLLXFXH
Validated NT Sequence
aatgnaagaa ctngagcagn cccgccgcgc cgcgcacgac cgcgcgctcg cgnttcaccg ncagcccgag cgtctcgtac cagtcgagca ggctgctgtg cgtgtcgggc atctngacgc cgtcgagcac gccganccng taggcgaaaa acgaaagcgg ccgcgacgcg gtgatcttcg aatcgagctg ccgcaggcta cccgccgccg cgttgcgcgg attggcgaat tcgcgctggc ccgccgcgcg ctgacgctcg ttcaagcgcg cgaaatcgcg cctgaacatg agcacttcgc cgcgcacgtc gagcatgcgc ggcacgcgct tgcccttgag cgtcagcgga atcgagcgga ccgtgcggat gttctcggtg acgtcttcgc ccgtcgtgcc gtcgccgcgc gtcgatgcct gcacgaagcg gccgttctcg tagcgcagcg agatcgccag gccgtcgaac ttcagctcgc acgcgtattc gacaggctcg gtcacggtgc cggcgaggtc ggtcggcttg tcgaggccgt cggcgacgcg cttgtcgaac gcgacgacgt cgtcgtcgga aaaaccgttg ttgagcgaca gcatcggcgt atcgtgaacg accggcgtga agccgctcgc gacctcgccg ccgacgcgct gcgtcggcga atcgggcgtg acgagatcgg ggtgctcggc ctcgatctgc tggagttcgg cgaagaggcg gtcgtactcg gcgtcgggca ggtccggctg gtcgagcacg taatatgcgt agttcgcccg ctcgagctcg gcgcgcagcc atgcggcgcg cttcgccggc tggccggcgg acggttcaac tggggatcgg gccatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMARSPVEPS AGQPAKRAAW LRAELERANY AYYVLDQPDL PDAEYDRLFA ELQQIEAEHP DLVTPDSPTQ RVGGEVASGF TPVVHDTPML SLNNGFSDDD VVAFDKRVAD GLDKPTDLAG TVTEPVEYAC ELKFDGLAIS LRYENGRFVQ ASTRGDGTTG EDVTENIRTV RSIPLTLKGK RVPRMLDVRG EVLMFRRDFA RLNERQRAAG QREFANPRNA AAGSLRQLDS KITASRPLSF FAYGIGVLDG VEMPDTHSSL LDWYETLGLP VNRERAVVRG AAGLLEFFHS VGARRESLPY DIDGVVYKVN RRDEQDRLGF VSRAPRFALA HKFPAQEALT KLVAIDVQVG RTGAITPVAR LEPVFVGGAT VTNATLHNED EVRRKDIRIG DTVIVRRAGD VIPEVVSAVF DRRPADAREF VMPTECPECG SRVERLPDEA IARCTGGLFC PAQRKQALWH FAQRRALDID GLGEKIIDQL VEQNLVRTPA DLFNLGFSTL VELDRFAEKS AQNLIDSLEK AKHTTLARFI YALGIRHVGE STAKDLAKHF GSLDPIMDAS IDELLEVNDV GPIVAESIHQ FFAEAHNRTV IEQLRAKGKV TWPEGPPAPR APQGVLAGKT VVLTGTLPTL TREAAKEMLE AAGAKVAGSV SKKTDYVVAG ADAGSKLAKA EELGIPVLDE AGMHKLLEGH AR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCCCGATCC CCAGTTGAAC CGTCCGCCGG CCAGCCGGCG AAGCGCGCCG CATGGCTGCG CGCCGAGCTC GAGCGGGCGA ACTACGCATA TTACGTGCTC GACCAGCCGG ACCTGCCCGA CGCCGAGTAC GACCGCCTCT TCGCCGAACT CCAGCAGATC GAGGCCGAGC ACCCCGATCT CGTCACGCCC GATTCGCCGA CGCAGCGCGT CGGCGGCGAG GTCGCGAGCG GCTTCACGCC GGTCGTTCAC GATACGCCGA TGCTGTCGCT CAACAACGGT TTTTCCGACG ACGACGTCGT CGCGTTCGAC AAGCGCGTCG CCGACGGCCT CGACAAGCCG ACCGACCTCG CCGGCACCGT GACCGAGCCT GTCGAATACG CGTGCGAGCT GAAGTTCGAC GGCCTGGCGA TCTCGCTGCG CTACGAGAAC GGCCGCTTCG TGCAGGCATC GACGCGCGGC GACGGCACGA CGGGCGAAGA CGTCACCGAG AACATCCGCA CGGTCCGCTC GATTCCGCTG ACGCTCAAGG GCAAGCGCGT GCCGCGCATG CTCGACGTGC GCGGCGAAGT GCTCATGTTC AGGCGCGATT TCGCGCGCTT GAACGAGCGT CAGCGCGCGG CGGGCCAGCG CGAATTCGCC AATCCGCGCA ACGCGGCGGC GGGTAGCCTG CGGCAGCTCG ATTCGAAGAT CACCGCGTCG CGGCCGCTTT CGTTTTTCGC CTACGGGATC GGCGTGCTCG ACGGCGTCGA GATGCCCGAC ACGCACAGCA GCCTGCTCGA CTGGTACGAG ACGCTCGGGC TGCCGGTGAA CCGCGAGCGC GCGGTCGTGC GCGGCGCGGC GGGCCTGCTC GAGTTCTTCC ATTCGGTGGG CGCGCGGCGC GAGTCGCTGC CGTACGACAT CGACGGCGTC GTCTACAAGG TGAATCGCCG CGACGAGCAG GATCGGCTCG GCTTCGTGTC GCGCGCGCCG CGCTTCGCGC TCGCGCACAA GTTCCCCGCG CAGGAGGCGC TGACGAAGCT CGTCGCGATC GACGTGCAGG TCGGCCGCAC GGGCGCGATC ACGCCGGTCG CGCGTCTCGA GCCCGTGTTC GTCGGCGGCG CGACCGTCAC GAACGCGACG CTGCACAACG AGGACGAGGT GCGCCGCAAG GACATCCGGA TCGGCGACAC GGTCATCGTG CGCCGCGCGG GCGACGTGAT TCCCGAAGTG GTGTCGGCCG TGTTCGACCG GCGTCCCGCC GACGCGCGCG AGTTCGTGAT GCCGACCGAG TGCCCGGAAT GCGGTTCGCG GGTCGAGCGA TTGCCCGACG AGGCGATCGC GCGCTGCACG GGCGGGCTCT TCTGCCCCGC GCAGCGCAAG CAGGCGCTCT GGCACTTCGC GCAGCGCCGC GCGCTCGACA TCGACGGGCT CGGCGAGAAG ATCATCGATC AGCTCGTCGA GCAGAACCTC GTGCGCACGC CGGCCGATCT GTTCAATCTC GGCTTCTCGA CGCTCGTCGA GCTCGATCGT TTTGCGGAGA AGTCCGCGCA GAACCTGATC GACTCGCTCG AAAAGGCGAA GCACACGACG CTCGCGCGCT TCATTTACGC GCTCGGCATC CGGCACGTCG GCGAATCGAC GGCGAAGGAC CTCGCGAAGC ACTTCGGCTC GCTCGATCCG ATCATGGACG CGTCGATCGA CGAATTGCTC GAAGTCAATG ACGTCGGGCC GATCGTCGCC GAATCGATCC ATCAGTTCTT CGCCGAGGCG CACAATCGCA CGGTGATCGA GCAACTGCGC GCGAAGGGCA AAGTCACGTG GCCCGAAGGG CCGCCCGCGC CGCGCGCGCC GCAGGGCGTG CTCGCGGGCA AGACCGTCGT GCTGACGGGC ACGCTGCCGA CGCTCACGCG GGAAGCCGCG AAGGAGATGC TCGAGGCGGC GGGCGCGAAG GTCGCGGGCT CGGTGTCGAA GAAGACCGAT TACGTCGTCG CGGGCGCCGA CGCGGGCAGC AAGCTCGCGA AAGCCGAGGA GCTCGGCATT CCGGTGCTGG ACGAAGCAGG CATGCACAAA CTTCTGGAGG GCCATGCGCG ATAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA