SSGCID: Target Page

ButhA.17945.a

30S ribosomal protein S3 (BTH_I3062) (rpsC)

CENTER ID:	ButhA.17945.a
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.17945.a.A1.GE32985	full length		1	266

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.5953
RefSeq:	YP_443566.1
UniProt:	Q2SU33

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGQKIHPTGF RLAVSRNWAS RWYANNNNFA AMLQEDIGVR EYLKKKLKNA SVGRVIIERP AKNARITIFS SRPGVVIGKK GEDIELLKTE LQRRMGVPVH VNIEEIRKPE TDAQLIADSI TQQLERRIMF RRAMKRAMQN AMRLGAQGIK IMSAGRLNGI EIARTEWYRE GRVPLHTLRA DIDYATSEAK TTYGVIGVKV WVYKGDTLGR NDAPVVEEVT EDKRPRRNAR PGDRRPRRDG EGGAPGARRG GPRRGAGKPE DGKTGE

NT Sequence

atgggacaga aaattcatcc gactggcttc cgcctggctg tcagccgcaa ttgggcttcg cgctggtacg cgaacaacaa caatttcgcg gcgatgcttc aggaagacat cggtgttcgt gagtacctga agaagaagct gaagaacgct tcggtcggcc gcgtcatcat tgagcgtccg gcgaagaacg cgcgcatcac gattttcagc tcgcgtccgg gcgtcgtcat cggcaagaag ggcgaggata tcgaactgct gaagacggaa ctgcaacgcc gcatgggtgt gccggttcac gtcaacatcg aagagatccg caagccggaa accgatgcgc aactgatcgc cgattcgatc acgcagcagc tcgagcgccg gatcatgttc cgccgcgcga tgaagcgcgc gatgcagaac gcgatgcgtc tgggtgccca gggcatcaag atcatgagcg ccggtcgtct gaacggcatc gaaatcgcgc gtaccgagtg gtaccgcgaa ggccgcgtgc ccctgcacac gctgcgcgcc gacatcgatt acgcgacttc ggaagcgaag acgacgtacg gcgtcatcgg cgtcaaggta tgggtgtaca agggcgacac gcttggccgc aacgatgcgc cggtggtcga ggaagtgacc gaagacaagc gtccgcgtcg caatgcgcgt ccgggcgacc gtcgtccgcg ccgtgacggc gaaggcggcg ctccgggcgc tcgccgtggc ggtccgcgcc gcggcgccgg caagccggaa gacggcaaga ctggagaata a

Details for ButhA.17945.a.A1.GE32985

HARVESTED ON:	8/16/2011
SEQUENCED ON:	8/23/2011
EXPECTED MW:	32kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	15
PERCENT COVERAGE:	13

Validated AA Sequence

MXRXXSVFSS XISSPFLXXX TPGREXKXXM RAFFXGRSMX

Validated NT Sequence

cgtcntcatt gagcgtccgg ngaagaacgc gcgcatcnnn attttcnnct cgcgtccggg cgtcnncntc ngcaagaagg gcgaggatat cnaactgctg aagacnganc tgcnacgccn catgggtgtg ccggttc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMGQKIHPTG FRLAVSRNWA SRWYANNNNF AAMLQEDIGV REYLKKKLKN ASVGRVIIER PAKNARITIF SSRPGVVIGK KGEDIELLKT ELQRRMGVPV HVNIEEIRKP ETDAQLIADS ITQQLERRIM FRRAMKRAMQ NAMRLGAQGI KIMSAGRLNG IEIARTEWYR EGRVPLHTLR ADIDYATSEA KTTYGVIGVK VWVYKGDTLG RNDAPVVEEV TEDKRPRRNA RPGDRRPRRD GEGGAPGARR GGPRRGAGKP EDGKTGE

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggacagaaa attcatccga ctggcttccg cctggctgtc agccgcaatt gggcttcgcg ctggtacgcg aacaacaaca atttcgcggc gatgcttcag gaagacatcg gtgttcgtga gtacctgaag aagaagctga agaacgcttc ggtcggccgc gtcatcattg agcgtccggc gaagaacgcg cgcatcacga ttttcagctc gcgtccgggc gtcgtcatcg gcaagaaggg cgaggatatc gaactgctga agacggaact gcaacgccgc atgggtgtgc cggttcacgt caacatcgaa gagatccgca agccggaaac cgatgcgcaa ctgatcgccg attcgatcac gcagcagctc gagcgccgga tcatgttccg ccgcgcgatg aagcgcgcga tgcagaacgc gatgcgtctg ggtgcccagg gcatcaagat catgagcgcc ggtcgtctga acggcatcga aatcgcgcgt accgagtggt accgcgaagg ccgcgtgccc ctgcacacgc tgcgcgccga catcgattac gcgacttcgg aagcgaagac gacgtacggc gtcatcggcg tcaaggtatg ggtgtacaag ggcgacacgc ttggccgcaa cgatgcgccg gtggtcgagg aagtgaccga agacaagcgt ccgcgtcgca atgcgcgtcc gggcgaccgt cgtccgcgcc gtgacggcga aggcggcgct ccgggcgctc gccgtggcgg tccgcgccgc ggcgccggca agccggaaga cggcaagact ggagaataaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa