ButhA.17943.a

Leucyl-tRNA synthetase (EC 6.1.1.4) (Leucine--tRNA ligase) (LeuRS) (BTH_I1211) (leuS)

CENTER ID: ButhA.17943.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.17943.a.A1.GE32983 full length 1 864
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.17943.a.A1.PS01227 full length 1 864
ButhA.17943.a.A1.PS01228 full length 1 864

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.3967
RefSeq: YP_441758.1
UniProt: Q2SZ91

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MHERYVPADV EAAAQSDWRA ADAYRSKEDA NRKKFYCVSM LPYPSGKLHM GHVRNYTIND VMYRYLRMNG YNTLMPMGWD AFGMPAENAA MANGVPPAQW TYDNIAYMKK QMQSMGLAID WSREVTTCKP DYYKWNQWLF LKMLEKGIAY KKTGTVNWDP VDQTVLANEQ VIDGRGWRSG ALVEKREIPM YYMRITQYAD ELLNDLDGLG WPERVKIMQQ NWIGKSFGVN FGFPYELDGE KKLLRVFTTR ADTIMGVTFC AIAAEHPLAA RLAQDKPELQ AFIDECKRGG VAEADIATME KKGVATGFSV SHPLTGAPVE VWIGNYVLMS YGEGAVMGVP AHDERDFAFA KKYGLPIRQV IAVEGETYST DAWQEWYGDK TRAVCVNSGK YDGLAYDAAV DAIAADLKAG GFGDKQITYR LRDWGISRQR YWGTPIPIIH CPSCGDVPVP EQDLPVVLPE DLVPDGTGNP LAKSDAFLNC ACPKCGAAAK RETDTMDTFV DSAWYFSRYA APDAQTMVDA RTDYWMPMDQ YIGGIEHAIL HLLYSRFWAK VMRDLGLVAF GEPAKNLLTQ GMVLNETFYR EDAAGKKTWY NPADVTVSFD DKGRPVGAVL KADGQPVVLG GIEKMSKSKN NGVDPQMLID HYGADTARLF TMFAAPPEQQ LEWSGAGVDG ASRFLRRVWA FGFANREALA VRAPFDVAQL AEADKTLRRE IHGVLKQADF DYQRLQYNTV VSAAMKMLNA IEGAKGATPA VLRETYGVLL RVLYPVVPHV TYELWKALGY ADEFGPLLDA PWPKVDEAAL EQAEIELVLQ INGKVRGALK VAKDASREAI EAAAVADEMF AKFAEGKPAK KIIVVPGRLV NVVV
NT Sequence
atgcacgaaa gatacgtacc cgccgacgtc gaagccgccg cccagagcga ctggcgcgca gccgatgcct accgctcgaa ggaagacgcg aaccgcaaga agttctactg cgtgtcgatg ctgccttacc cgtcgggcaa gctgcacatg ggtcacgtgc gcaactacac gatcaacgac gtgatgtacc gctatctgcg gatgaacggc tacaacacgc tgatgccgat gggttgggac gcgttcggga tgccggccga gaacgccgcg atggccaacg gcgtgccgcc cgcgcagtgg acgtacgaca acatcgcgta catgaagaag cagatgcagt cgatgggcct cgcgatcgac tggtcgcgcg aggtcacgac ctgcaagccc gactactaca agtggaacca gtggctgttc ctgaagatgc tcgagaaggg catcgcgtac aagaagaccg gcaccgtgaa ctgggacccg gtcgaccaga ccgtgctcgc gaacgagcag gtgatcgacg ggcgcggctg gcgctcgggc gcgctcgtcg agaagcgcga gatcccgatg tactacatgc ggatcacgca gtacgccgat gaactgctga acgatctcga cggcctcggc tggcccgagc gcgtgaagat catgcagcag aactggatcg gcaagagctt cggcgtgaac ttcggcttcc cgtacgagct cgacggcgag aagaagctgt tgcgcgtgtt cacgacgcgc gcggacacga tcatgggcgt cacgttctgc gcgatcgcgg ccgagcatcc gctcgccgcg cggcttgcgc aggacaagcc ggaactgcag gcgttcatcg acgaatgcaa gcgcggcggc gttgccgagg ccgacatcgc gacgatggag aagaagggcg tcgcgacggg tttttcggtg tcgcatccgc tcacgggcgc accggtcgag gtgtggatcg gcaactacgt gctgatgagc tacggcgagg gcgcggtgat gggcgtgccg gcgcacgacg agcgcgactt cgcgttcgcg aagaagtacg gcctgccgat ccggcaggtg attgccgtgg agggcgagac gtactcgacc gacgcgtggc aggagtggta cggcgacaag acgcgcgccg tgtgcgtgaa cagcggcaag tacgacggcc tcgcgtacga cgcggccgtc gacgcgatcg cggccgacct gaaggcgggc ggcttcggcg acaagcagat cacgtaccgc ttgcgcgact ggggcatttc ccgccagcgc tactggggca cgccgattcc gatcatccac tgcccgtcgt gcggcgacgt gccggtgccg gaacaggacc tgcccgtcgt gctgccggaa gacctcgtgc cggacggcac gggcaacccg ctcgcgaaat ccgacgcgtt cctgaactgc gcgtgcccga agtgcggcgc ggcggcgaag cgcgaaacgg acacgatgga cacgttcgtc gattccgcgt ggtacttctc gcgctacgcg gcgcccgacg cgcagacgat ggtcgacgcg cgcaccgact actggatgcc gatggaccag tacatcggcg gcatcgagca cgcgatcctg cacctgctgt actcgcgctt ctgggcgaag gtgatgcgcg acctcggcct cgtcgcgttc ggcgagccgg cgaagaacct gctcacgcag gggatggtgc tcaacgagac gttctaccgc gaggacgcgg cgggcaagaa gacctggtac aacccggccg acgtcaccgt gtcgttcgac gacaaggggc gccccgtcgg cgcggtgctg aaggcggacg ggcagccggt cgtgctgggc ggcatcgaga agatgtcgaa gtcgaagaac aacggcgtcg atccgcagat gctgatcgac cattacggcg ccgacaccgc gcgcctcttc acgatgttcg cggcgccccc cgagcagcag ctcgagtggt cgggcgcggg cgtcgacggc gcgagccgct tcctgcgccg cgtgtgggcg ttcggcttcg cgaaccgcga agcgctcgcc gtgcgcgcgc cgttcgacgt cgcgcaactc gccgaagcgg acaagacgct gcgccgcgag atccacggcg tgctgaagca ggccgatttc gactaccagc gcctgcagta caacacggtc gtgtcggccg cgatgaagat gctcaacgcg atcgaaggcg cgaagggcgc gacgcccgcc gtgctgcgcg agacctatgg cgtgctgctg cgcgtgctgt atccggtcgt gccgcacgtc acgtacgagc tgtggaaggc gctcggctac gcggacgagt ttggcccgct tctcgacgcg ccgtggccga aggtcgacga ggccgcgctc gagcaggccg agatcgagct cgtgctgcag atcaacggca aggtgcgcgg cgcgctgaag gtggcgaagg acgcgagccg cgaggcgatc gaggcggcgg ccgtcgccga cgagatgttc gcgaaattcg ccgaaggcaa gccggcgaag aagatcattg tcgtgccggg ccgtctcgtg aacgtcgtcg tctga
Details for ButhA.17943.a.A1.GE32983
HARVESTED ON: 8/16/2011
SEQUENCED ON: 8/22/2011
EXPECTED MW: 98kDa
OBSERVED MW: 98kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 69
PERCENT COVERAGE: 44
Validated AA Sequence
MXHHXXHHMG TLEAQTQGPG SMHERYVPAD VEXAAQSDWX AADAYRSKED ANRKKFYCVS MLPYPSGKLH MGHVRNYTIN DVMYRYLRMN GYNTLMPMGW DAFGMPXENA AMANGVPPAQ WTYDNIAYMK KXMQSMGLAI DWSREVTTCK PDYYKWNQWL FLKMLXKGIA YXKTGTVNWD PVDQTVLXNE XVIDGRGWRS GALVXKREIP MYYMRXXXYA XXLLNDLDXL GWXERVXIMQ QNWIGKSFXV XFXFPYELDX XKXLLRVFTX RADTIMGXTF CAIXXXHPLX ARLAXXKPXT AXVHRXXQAR RRCRGRXAXX XXXGVXXGFS VSHXSXXXXS RXXSAXTXXX XXXGXXXXXX XXTXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXX
Validated NT Sequence
attttgttta ctttannnag gagatatacc atggntcatc accntcncca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcacg aaagatacgt acccgccgac gtcgaanccg ccgcccagag cgactggngc gcagccgatg cctaccgctc gaaggaagac gcgaaccgca agaagttcta ctgcgtgtcg atgctgcctt acccgtcggg caagctgcac atgggtcacg tgcgcaacta cacgatcaac gacgtgatgt accgctatct gcggatgaac ggctacaaca cgctgatgcc gatgggttgg gacgcgttcg ggatgccggn cgagaacgcc gcgatggcca acggcgtgcc gcccgcgcag tggacgtacg acaacatcgc gtacatgaag aagcanatgc agtcgatggg cctcgcgatc gactggtcgc gcgaggtcac gacctgcaag cccgactact acaagtggaa ccagtggctg ttcctgaaga tgctcnagaa gggcatcgcg tacannaaga ccggcaccgt gaactgggac ccggtcgacc agaccgtgct cncnaacgag naggtgatcg acgggcgcgg ctggcgctcg ggcgcgctcg tnnagaagcg cgagatcccg atgtactaca tgcggatnan gcnntacgcc natnaactgc tgaacgatct cgacngcctc ggctggnccg agcgcgtgna gatcatgcag cagaactgga tcggcaagag cttcngcgtg nacttcngct tcccgtacga gctcgacngc nagaagaanc tgttgcgcgt gttcacnann cgcgcggaca cnatcatggg cntcacgttc tgcgcgatcg nggncganca tccgctcgnc gcgcggcttg cncanganaa gccngnaact gcangcgttc atcgacnant gcaagcgcgg cggcgttgcc gaggccgant cgcnncgann nannanaang gcgtcncgnc nggnttttcg gtgtcgcatc nntcnnnggn ncnccnntcg aggnnngnat cggcanntac nnnnngatna nctnnnnnag ggnnnnnnnn angnnnnnnn nggcncacnn nnagnnntnc ncnntnnnnn naannantnn nnnnnnncna nnnnnncagn nnattnncnn tnnnggnnnn ncntnnnnnn ncnannnnnn nnnnngnnnn nt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMHERYVPAD VEAAAQSDWR AADAYRSKED ANRKKFYCVS MLPYPSGKLH MGHVRNYTIN DVMYRYLRMN GYNTLMPMGW DAFGMPAENA AMANGVPPAQ WTYDNIAYMK KQMQSMGLAI DWSREVTTCK PDYYKWNQWL FLKMLEKGIA YKKTGTVNWD PVDQTVLANE QVIDGRGWRS GALVEKREIP MYYMRITQYA DELLNDLDGL GWPERVKIMQ QNWIGKSFGV NFGFPYELDG EKKLLRVFTT RADTIMGVTF CAIAAEHPLA ARLAQDKPEL QAFIDECKRG GVAEADIATM EKKGVATGFS VSHPLTGAPV EVWIGNYVLM SYGEGAVMGV PAHDERDFAF AKKYGLPIRQ VIAVEGETYS TDAWQEWYGD KTRAVCVNSG KYDGLAYDAA VDAIAADLKA GGFGDKQITY RLRDWGISRQ RYWGTPIPII HCPSCGDVPV PEQDLPVVLP EDLVPDGTGN PLAKSDAFLN CACPKCGAAA KRETDTMDTF VDSAWYFSRY AAPDAQTMVD ARTDYWMPMD QYIGGIEHAI LHLLYSRFWA KVMRDLGLVA FGEPAKNLLT QGMVLNETFY REDAAGKKTW YNPADVTVSF DDKGRPVGAV LKADGQPVVL GGIEKMSKSK NNGVDPQMLI DHYGADTARL FTMFAAPPEQ QLEWSGAGVD GASRFLRRVW AFGFANREAL AVRAPFDVAQ LAEADKTLRR EIHGVLKQAD FDYQRLQYNT VVSAAMKMLN AIEGAKGATP AVLRETYGVL LRVLYPVVPH VTYELWKALG YADEFGPLLD APWPKVDEAA LEQAEIELVL QINGKVRGAL KVAKDASREA IEAAAVADEM FAKFAEGKPA KKIIVVPGRL VNVVV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcacgaaaga tacgtacccg ccgacgtcga agccgccgcc cagagcgact ggcgcgcagc cgatgcctac cgctcgaagg aagacgcgaa ccgcaagaag ttctactgcg tgtcgatgct gccttacccg tcgggcaagc tgcacatggg tcacgtgcgc aactacacga tcaacgacgt gatgtaccgc tatctgcgga tgaacggcta caacacgctg atgccgatgg gttgggacgc gttcgggatg ccggccgaga acgccgcgat ggccaacggc gtgccgcccg cgcagtggac gtacgacaac atcgcgtaca tgaagaagca gatgcagtcg atgggcctcg cgatcgactg gtcgcgcgag gtcacgacct gcaagcccga ctactacaag tggaaccagt ggctgttcct gaagatgctc gagaagggca tcgcgtacaa gaagaccggc accgtgaact gggacccggt cgaccagacc gtgctcgcga acgagcaggt gatcgacggg cgcggctggc gctcgggcgc gctcgtcgag aagcgcgaga tcccgatgta ctacatgcgg atcacgcagt acgccgatga actgctgaac gatctcgacg gcctcggctg gcccgagcgc gtgaagatca tgcagcagaa ctggatcggc aagagcttcg gcgtgaactt cggcttcccg tacgagctcg acggcgagaa gaagctgttg cgcgtgttca cgacgcgcgc ggacacgatc atgggcgtca cgttctgcgc gatcgcggcc gagcatccgc tcgccgcgcg gcttgcgcag gacaagccgg aactgcaggc gttcatcgac gaatgcaagc gcggcggcgt tgccgaggcc gacatcgcga cgatggagaa gaagggcgtc gcgacgggtt tttcggtgtc gcatccgctc acgggcgcac cggtcgaggt gtggatcggc aactacgtgc tgatgagcta cggcgagggc gcggtgatgg gcgtgccggc gcacgacgag cgcgacttcg cgttcgcgaa gaagtacggc ctgccgatcc ggcaggtgat tgccgtggag ggcgagacgt actcgaccga cgcgtggcag gagtggtacg gcgacaagac gcgcgccgtg tgcgtgaaca gcggcaagta cgacggcctc gcgtacgacg cggccgtcga cgcgatcgcg gccgacctga aggcgggcgg cttcggcgac aagcagatca cgtaccgctt gcgcgactgg ggcatttccc gccagcgcta ctggggcacg ccgattccga tcatccactg cccgtcgtgc ggcgacgtgc cggtgccgga acaggacctg cccgtcgtgc tgccggaaga cctcgtgccg gacggcacgg gcaacccgct cgcgaaatcc gacgcgttcc tgaactgcgc gtgcccgaag tgcggcgcgg cggcgaagcg cgaaacggac acgatggaca cgttcgtcga ttccgcgtgg tacttctcgc gctacgcggc gcccgacgcg cagacgatgg tcgacgcgcg caccgactac tggatgccga tggaccagta catcggcggc atcgagcacg cgatcctgca cctgctgtac tcgcgcttct gggcgaaggt gatgcgcgac ctcggcctcg tcgcgttcgg cgagccggcg aagaacctgc tcacgcaggg gatggtgctc aacgagacgt tctaccgcga ggacgcggcg ggcaagaaga cctggtacaa cccggccgac gtcaccgtgt cgttcgacga caaggggcgc cccgtcggcg cggtgctgaa ggcggacggg cagccggtcg tgctgggcgg catcgagaag atgtcgaagt cgaagaacaa cggcgtcgat ccgcagatgc tgatcgacca ttacggcgcc gacaccgcgc gcctcttcac gatgttcgcg gcgccccccg agcagcagct cgagtggtcg ggcgcgggcg tcgacggcgc gagccgcttc ctgcgccgcg tgtgggcgtt cggcttcgcg aaccgcgaag cgctcgccgt gcgcgcgccg ttcgacgtcg cgcaactcgc cgaagcggac aagacgctgc gccgcgagat ccacggcgtg ctgaagcagg ccgatttcga ctaccagcgc ctgcagtaca acacggtcgt gtcggccgcg atgaagatgc tcaacgcgat cgaaggcgcg aagggcgcga cgcccgccgt gctgcgcgag acctatggcg tgctgctgcg cgtgctgtat ccggtcgtgc cgcacgtcac gtacgagctg tggaaggcgc tcggctacgc ggacgagttt ggcccgcttc tcgacgcgcc gtggccgaag gtcgacgagg ccgcgctcga gcaggccgag atcgagctcg tgctgcagat caacggcaag gtgcgcggcg cgctgaaggt ggcgaaggac gcgagccgcg aggcgatcga ggcggcggcc gtcgccgacg agatgttcgc gaaattcgcc gaaggcaagc cggcgaagaa gatcattgtc gtgccgggcc gtctcgtgaa cgtcgtcgtc taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for ButhA.17943.a.A1.PS01227
PURIFICATION DATe: 10/4/2011
CONCENTRATION: 33.67mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 100µl
PERCENT IDENTITY: 69
PERCENT COVERAGE: 44
Protocol Notes
notes unavailable
Validated AA Sequence
MXHHXXHHMG TLEAQTQGPG SMHERYVPAD VEXAAQSDWX AADAYRSKED ANRKKFYCVS MLPYPSGKLH MGHVRNYTIN DVMYRYLRMN GYNTLMPMGW DAFGMPXENA AMANGVPPAQ WTYDNIAYMK KXMQSMGLAI DWSREVTTCK PDYYKWNQWL FLKMLXKGIA YXKTGTVNWD PVDQTVLXNE XVIDGRGWRS GALVXKREIP MYYMRXXXYA XXLLNDLDXL GWXERVXIMQ QNWIGKSFXV XFXFPYELDX XKXLLRVFTX RADTIMGXTF CAIXXXHPLX ARLAXXKPXT AXVHRXXQAR RRCRGRXAXX XXXGVXXGFS VSHXSXXXXS RXXSAXTXXX XXXGXXXXXX XXTXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXX
Validated NT Sequence
attttgttta ctttannnag gagatatacc atggntcatc accntcncca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcacg aaagatacgt acccgccgac gtcgaanccg ccgcccagag cgactggngc gcagccgatg cctaccgctc gaaggaagac gcgaaccgca agaagttcta ctgcgtgtcg atgctgcctt acccgtcggg caagctgcac atgggtcacg tgcgcaacta cacgatcaac gacgtgatgt accgctatct gcggatgaac ggctacaaca cgctgatgcc gatgggttgg gacgcgttcg ggatgccggn cgagaacgcc gcgatggcca acggcgtgcc gcccgcgcag tggacgtacg acaacatcgc gtacatgaag aagcanatgc agtcgatggg cctcgcgatc gactggtcgc gcgaggtcac gacctgcaag cccgactact acaagtggaa ccagtggctg ttcctgaaga tgctcnagaa gggcatcgcg tacannaaga ccggcaccgt gaactgggac ccggtcgacc agaccgtgct cncnaacgag naggtgatcg acgggcgcgg ctggcgctcg ggcgcgctcg tnnagaagcg cgagatcccg atgtactaca tgcggatnan gcnntacgcc natnaactgc tgaacgatct cgacngcctc ggctggnccg agcgcgtgna gatcatgcag cagaactgga tcggcaagag cttcngcgtg nacttcngct tcccgtacga gctcgacngc nagaagaanc tgttgcgcgt gttcacnann cgcgcggaca cnatcatggg cntcacgttc tgcgcgatcg nggncganca tccgctcgnc gcgcggcttg cncanganaa gccngnaact gcangcgttc atcgacnant gcaagcgcgg cggcgttgcc gaggccgant cgcnncgann nannanaang gcgtcncgnc nggnttttcg gtgtcgcatc nntcnnnggn ncnccnntcg aggnnngnat cggcanntac nnnnngatna nctnnnnnag ggnnnnnnnn angnnnnnnn nggcncacnn nnagnnntnc ncnntnnnnn naannantnn nnnnnnncna nnnnnncagn nnattnncnn tnnnggnnnn ncntnnnnnn ncnannnnnn nnnnngnnnn nt
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMHERYVPAD VEAAAQSDWR AADAYRSKED ANRKKFYCVS MLPYPSGKLH MGHVRNYTIN DVMYRYLRMN GYNTLMPMGW DAFGMPAENA AMANGVPPAQ WTYDNIAYMK KQMQSMGLAI DWSREVTTCK PDYYKWNQWL FLKMLEKGIA YKKTGTVNWD PVDQTVLANE QVIDGRGWRS GALVEKREIP MYYMRITQYA DELLNDLDGL GWPERVKIMQ QNWIGKSFGV NFGFPYELDG EKKLLRVFTT RADTIMGVTF CAIAAEHPLA ARLAQDKPEL QAFIDECKRG GVAEADIATM EKKGVATGFS VSHPLTGAPV EVWIGNYVLM SYGEGAVMGV PAHDERDFAF AKKYGLPIRQ VIAVEGETYS TDAWQEWYGD KTRAVCVNSG KYDGLAYDAA VDAIAADLKA GGFGDKQITY RLRDWGISRQ RYWGTPIPII HCPSCGDVPV PEQDLPVVLP EDLVPDGTGN PLAKSDAFLN CACPKCGAAA KRETDTMDTF VDSAWYFSRY AAPDAQTMVD ARTDYWMPMD QYIGGIEHAI LHLLYSRFWA KVMRDLGLVA FGEPAKNLLT QGMVLNETFY REDAAGKKTW YNPADVTVSF DDKGRPVGAV LKADGQPVVL GGIEKMSKSK NNGVDPQMLI DHYGADTARL FTMFAAPPEQ QLEWSGAGVD GASRFLRRVW AFGFANREAL AVRAPFDVAQ LAEADKTLRR EIHGVLKQAD FDYQRLQYNT VVSAAMKMLN AIEGAKGATP AVLRETYGVL LRVLYPVVPH VTYELWKALG YADEFGPLLD APWPKVDEAA LEQAEIELVL QINGKVRGAL KVAKDASREA IEAAAVADEM FAKFAEGKPA KKIIVVPGRL VNVVV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCACGAAAGA TACGTACCCG CCGACGTCGA AGCCGCCGCC CAGAGCGACT GGCGCGCAGC CGATGCCTAC CGCTCGAAGG AAGACGCGAA CCGCAAGAAG TTCTACTGCG TGTCGATGCT GCCTTACCCG TCGGGCAAGC TGCACATGGG TCACGTGCGC AACTACACGA TCAACGACGT GATGTACCGC TATCTGCGGA TGAACGGCTA CAACACGCTG ATGCCGATGG GTTGGGACGC GTTCGGGATG CCGGCCGAGA ACGCCGCGAT GGCCAACGGC GTGCCGCCCG CGCAGTGGAC GTACGACAAC ATCGCGTACA TGAAGAAGCA GATGCAGTCG ATGGGCCTCG CGATCGACTG GTCGCGCGAG GTCACGACCT GCAAGCCCGA CTACTACAAG TGGAACCAGT GGCTGTTCCT GAAGATGCTC GAGAAGGGCA TCGCGTACAA GAAGACCGGC ACCGTGAACT GGGACCCGGT CGACCAGACC GTGCTCGCGA ACGAGCAGGT GATCGACGGG CGCGGCTGGC GCTCGGGCGC GCTCGTCGAG AAGCGCGAGA TCCCGATGTA CTACATGCGG ATCACGCAGT ACGCCGATGA ACTGCTGAAC GATCTCGACG GCCTCGGCTG GCCCGAGCGC GTGAAGATCA TGCAGCAGAA CTGGATCGGC AAGAGCTTCG GCGTGAACTT CGGCTTCCCG TACGAGCTCG ACGGCGAGAA GAAGCTGTTG CGCGTGTTCA CGACGCGCGC GGACACGATC ATGGGCGTCA CGTTCTGCGC GATCGCGGCC GAGCATCCGC TCGCCGCGCG GCTTGCGCAG GACAAGCCGG AACTGCAGGC GTTCATCGAC GAATGCAAGC GCGGCGGCGT TGCCGAGGCC GACATCGCGA CGATGGAGAA GAAGGGCGTC GCGACGGGTT TTTCGGTGTC GCATCCGCTC ACGGGCGCAC CGGTCGAGGT GTGGATCGGC AACTACGTGC TGATGAGCTA CGGCGAGGGC GCGGTGATGG GCGTGCCGGC GCACGACGAG CGCGACTTCG CGTTCGCGAA GAAGTACGGC CTGCCGATCC GGCAGGTGAT TGCCGTGGAG GGCGAGACGT ACTCGACCGA CGCGTGGCAG GAGTGGTACG GCGACAAGAC GCGCGCCGTG TGCGTGAACA GCGGCAAGTA CGACGGCCTC GCGTACGACG CGGCCGTCGA CGCGATCGCG GCCGACCTGA AGGCGGGCGG CTTCGGCGAC AAGCAGATCA CGTACCGCTT GCGCGACTGG GGCATTTCCC GCCAGCGCTA CTGGGGCACG CCGATTCCGA TCATCCACTG CCCGTCGTGC GGCGACGTGC CGGTGCCGGA ACAGGACCTG CCCGTCGTGC TGCCGGAAGA CCTCGTGCCG GACGGCACGG GCAACCCGCT CGCGAAATCC GACGCGTTCC TGAACTGCGC GTGCCCGAAG TGCGGCGCGG CGGCGAAGCG CGAAACGGAC ACGATGGACA CGTTCGTCGA TTCCGCGTGG TACTTCTCGC GCTACGCGGC GCCCGACGCG CAGACGATGG TCGACGCGCG CACCGACTAC TGGATGCCGA TGGACCAGTA CATCGGCGGC ATCGAGCACG CGATCCTGCA CCTGCTGTAC TCGCGCTTCT GGGCGAAGGT GATGCGCGAC CTCGGCCTCG TCGCGTTCGG CGAGCCGGCG AAGAACCTGC TCACGCAGGG GATGGTGCTC AACGAGACGT TCTACCGCGA GGACGCGGCG GGCAAGAAGA CCTGGTACAA CCCGGCCGAC GTCACCGTGT CGTTCGACGA CAAGGGGCGC CCCGTCGGCG CGGTGCTGAA GGCGGACGGG CAGCCGGTCG TGCTGGGCGG CATCGAGAAG ATGTCGAAGT CGAAGAACAA CGGCGTCGAT CCGCAGATGC TGATCGACCA TTACGGCGCC GACACCGCGC GCCTCTTCAC GATGTTCGCG GCGCCCCCCG AGCAGCAGCT CGAGTGGTCG GGCGCGGGCG TCGACGGCGC GAGCCGCTTC CTGCGCCGCG TGTGGGCGTT CGGCTTCGCG AACCGCGAAG CGCTCGCCGT GCGCGCGCCG TTCGACGTCG CGCAACTCGC CGAAGCGGAC AAGACGCTGC GCCGCGAGAT CCACGGCGTG CTGAAGCAGG CCGATTTCGA CTACCAGCGC CTGCAGTACA ACACGGTCGT GTCGGCCGCG ATGAAGATGC TCAACGCGAT CGAAGGCGCG AAGGGCGCGA CGCCCGCCGT GCTGCGCGAG ACCTATGGCG TGCTGCTGCG CGTGCTGTAT CCGGTCGTGC CGCACGTCAC GTACGAGCTG TGGAAGGCGC TCGGCTACGC GGACGAGTTT GGCCCGCTTC TCGACGCGCC GTGGCCGAAG GTCGACGAGG CCGCGCTCGA GCAGGCCGAG ATCGAGCTCG TGCTGCAGAT CAACGGCAAG GTGCGCGGCG CGCTGAAGGT GGCGAAGGAC GCGAGCCGCG AGGCGATCGA GGCGGCGGCC GTCGCCGACG AGATGTTCGC GAAATTCGCC GAAGGCAAGC CGGCGAAGAA GATCATTGTC GTGCCGGGCC GTCTCGTGAA CGTCGTCGTC TAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA
Details for ButhA.17943.a.A1.PS01228
PURIFICATION DATe: 10/4/2011
CONCENTRATION: 40.68mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 100µl
PERCENT IDENTITY: 69
PERCENT COVERAGE: 44
Protocol Notes
notes unavailable
Validated AA Sequence
MXHHXXHHMG TLEAQTQGPG SMHERYVPAD VEXAAQSDWX AADAYRSKED ANRKKFYCVS MLPYPSGKLH MGHVRNYTIN DVMYRYLRMN GYNTLMPMGW DAFGMPXENA AMANGVPPAQ WTYDNIAYMK KXMQSMGLAI DWSREVTTCK PDYYKWNQWL FLKMLXKGIA YXKTGTVNWD PVDQTVLXNE XVIDGRGWRS GALVXKREIP MYYMRXXXYA XXLLNDLDXL GWXERVXIMQ QNWIGKSFXV XFXFPYELDX XKXLLRVFTX RADTIMGXTF CAIXXXHPLX ARLAXXKPXT AXVHRXXQAR RRCRGRXAXX XXXGVXXGFS VSHXSXXXXS RXXSAXTXXX XXXGXXXXXX XXTXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXX
Validated NT Sequence
attttgttta ctttannnag gagatatacc atggntcatc accntcncca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcacg aaagatacgt acccgccgac gtcgaanccg ccgcccagag cgactggngc gcagccgatg cctaccgctc gaaggaagac gcgaaccgca agaagttcta ctgcgtgtcg atgctgcctt acccgtcggg caagctgcac atgggtcacg tgcgcaacta cacgatcaac gacgtgatgt accgctatct gcggatgaac ggctacaaca cgctgatgcc gatgggttgg gacgcgttcg ggatgccggn cgagaacgcc gcgatggcca acggcgtgcc gcccgcgcag tggacgtacg acaacatcgc gtacatgaag aagcanatgc agtcgatggg cctcgcgatc gactggtcgc gcgaggtcac gacctgcaag cccgactact acaagtggaa ccagtggctg ttcctgaaga tgctcnagaa gggcatcgcg tacannaaga ccggcaccgt gaactgggac ccggtcgacc agaccgtgct cncnaacgag naggtgatcg acgggcgcgg ctggcgctcg ggcgcgctcg tnnagaagcg cgagatcccg atgtactaca tgcggatnan gcnntacgcc natnaactgc tgaacgatct cgacngcctc ggctggnccg agcgcgtgna gatcatgcag cagaactgga tcggcaagag cttcngcgtg nacttcngct tcccgtacga gctcgacngc nagaagaanc tgttgcgcgt gttcacnann cgcgcggaca cnatcatggg cntcacgttc tgcgcgatcg nggncganca tccgctcgnc gcgcggcttg cncanganaa gccngnaact gcangcgttc atcgacnant gcaagcgcgg cggcgttgcc gaggccgant cgcnncgann nannanaang gcgtcncgnc nggnttttcg gtgtcgcatc nntcnnnggn ncnccnntcg aggnnngnat cggcanntac nnnnngatna nctnnnnnag ggnnnnnnnn angnnnnnnn nggcncacnn nnagnnntnc ncnntnnnnn naannantnn nnnnnnncna nnnnnncagn nnattnncnn tnnnggnnnn ncntnnnnnn ncnannnnnn nnnnngnnnn nt
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMHERYVPAD VEAAAQSDWR AADAYRSKED ANRKKFYCVS MLPYPSGKLH MGHVRNYTIN DVMYRYLRMN GYNTLMPMGW DAFGMPAENA AMANGVPPAQ WTYDNIAYMK KQMQSMGLAI DWSREVTTCK PDYYKWNQWL FLKMLEKGIA YKKTGTVNWD PVDQTVLANE QVIDGRGWRS GALVEKREIP MYYMRITQYA DELLNDLDGL GWPERVKIMQ QNWIGKSFGV NFGFPYELDG EKKLLRVFTT RADTIMGVTF CAIAAEHPLA ARLAQDKPEL QAFIDECKRG GVAEADIATM EKKGVATGFS VSHPLTGAPV EVWIGNYVLM SYGEGAVMGV PAHDERDFAF AKKYGLPIRQ VIAVEGETYS TDAWQEWYGD KTRAVCVNSG KYDGLAYDAA VDAIAADLKA GGFGDKQITY RLRDWGISRQ RYWGTPIPII HCPSCGDVPV PEQDLPVVLP EDLVPDGTGN PLAKSDAFLN CACPKCGAAA KRETDTMDTF VDSAWYFSRY AAPDAQTMVD ARTDYWMPMD QYIGGIEHAI LHLLYSRFWA KVMRDLGLVA FGEPAKNLLT QGMVLNETFY REDAAGKKTW YNPADVTVSF DDKGRPVGAV LKADGQPVVL GGIEKMSKSK NNGVDPQMLI DHYGADTARL FTMFAAPPEQ QLEWSGAGVD GASRFLRRVW AFGFANREAL AVRAPFDVAQ LAEADKTLRR EIHGVLKQAD FDYQRLQYNT VVSAAMKMLN AIEGAKGATP AVLRETYGVL LRVLYPVVPH VTYELWKALG YADEFGPLLD APWPKVDEAA LEQAEIELVL QINGKVRGAL KVAKDASREA IEAAAVADEM FAKFAEGKPA KKIIVVPGRL VNVVV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCACGAAAGA TACGTACCCG CCGACGTCGA AGCCGCCGCC CAGAGCGACT GGCGCGCAGC CGATGCCTAC CGCTCGAAGG AAGACGCGAA CCGCAAGAAG TTCTACTGCG TGTCGATGCT GCCTTACCCG TCGGGCAAGC TGCACATGGG TCACGTGCGC AACTACACGA TCAACGACGT GATGTACCGC TATCTGCGGA TGAACGGCTA CAACACGCTG ATGCCGATGG GTTGGGACGC GTTCGGGATG CCGGCCGAGA ACGCCGCGAT GGCCAACGGC GTGCCGCCCG CGCAGTGGAC GTACGACAAC ATCGCGTACA TGAAGAAGCA GATGCAGTCG ATGGGCCTCG CGATCGACTG GTCGCGCGAG GTCACGACCT GCAAGCCCGA CTACTACAAG TGGAACCAGT GGCTGTTCCT GAAGATGCTC GAGAAGGGCA TCGCGTACAA GAAGACCGGC ACCGTGAACT GGGACCCGGT CGACCAGACC GTGCTCGCGA ACGAGCAGGT GATCGACGGG CGCGGCTGGC GCTCGGGCGC GCTCGTCGAG AAGCGCGAGA TCCCGATGTA CTACATGCGG ATCACGCAGT ACGCCGATGA ACTGCTGAAC GATCTCGACG GCCTCGGCTG GCCCGAGCGC GTGAAGATCA TGCAGCAGAA CTGGATCGGC AAGAGCTTCG GCGTGAACTT CGGCTTCCCG TACGAGCTCG ACGGCGAGAA GAAGCTGTTG CGCGTGTTCA CGACGCGCGC GGACACGATC ATGGGCGTCA CGTTCTGCGC GATCGCGGCC GAGCATCCGC TCGCCGCGCG GCTTGCGCAG GACAAGCCGG AACTGCAGGC GTTCATCGAC GAATGCAAGC GCGGCGGCGT TGCCGAGGCC GACATCGCGA CGATGGAGAA GAAGGGCGTC GCGACGGGTT TTTCGGTGTC GCATCCGCTC ACGGGCGCAC CGGTCGAGGT GTGGATCGGC AACTACGTGC TGATGAGCTA CGGCGAGGGC GCGGTGATGG GCGTGCCGGC GCACGACGAG CGCGACTTCG CGTTCGCGAA GAAGTACGGC CTGCCGATCC GGCAGGTGAT TGCCGTGGAG GGCGAGACGT ACTCGACCGA CGCGTGGCAG GAGTGGTACG GCGACAAGAC GCGCGCCGTG TGCGTGAACA GCGGCAAGTA CGACGGCCTC GCGTACGACG CGGCCGTCGA CGCGATCGCG GCCGACCTGA AGGCGGGCGG CTTCGGCGAC AAGCAGATCA CGTACCGCTT GCGCGACTGG GGCATTTCCC GCCAGCGCTA CTGGGGCACG CCGATTCCGA TCATCCACTG CCCGTCGTGC GGCGACGTGC CGGTGCCGGA ACAGGACCTG CCCGTCGTGC TGCCGGAAGA CCTCGTGCCG GACGGCACGG GCAACCCGCT CGCGAAATCC GACGCGTTCC TGAACTGCGC GTGCCCGAAG TGCGGCGCGG CGGCGAAGCG CGAAACGGAC ACGATGGACA CGTTCGTCGA TTCCGCGTGG TACTTCTCGC GCTACGCGGC GCCCGACGCG CAGACGATGG TCGACGCGCG CACCGACTAC TGGATGCCGA TGGACCAGTA CATCGGCGGC ATCGAGCACG CGATCCTGCA CCTGCTGTAC TCGCGCTTCT GGGCGAAGGT GATGCGCGAC CTCGGCCTCG TCGCGTTCGG CGAGCCGGCG AAGAACCTGC TCACGCAGGG GATGGTGCTC AACGAGACGT TCTACCGCGA GGACGCGGCG GGCAAGAAGA CCTGGTACAA CCCGGCCGAC GTCACCGTGT CGTTCGACGA CAAGGGGCGC CCCGTCGGCG CGGTGCTGAA GGCGGACGGG CAGCCGGTCG TGCTGGGCGG CATCGAGAAG ATGTCGAAGT CGAAGAACAA CGGCGTCGAT CCGCAGATGC TGATCGACCA TTACGGCGCC GACACCGCGC GCCTCTTCAC GATGTTCGCG GCGCCCCCCG AGCAGCAGCT CGAGTGGTCG GGCGCGGGCG TCGACGGCGC GAGCCGCTTC CTGCGCCGCG TGTGGGCGTT CGGCTTCGCG AACCGCGAAG CGCTCGCCGT GCGCGCGCCG TTCGACGTCG CGCAACTCGC CGAAGCGGAC AAGACGCTGC GCCGCGAGAT CCACGGCGTG CTGAAGCAGG CCGATTTCGA CTACCAGCGC CTGCAGTACA ACACGGTCGT GTCGGCCGCG ATGAAGATGC TCAACGCGAT CGAAGGCGCG AAGGGCGCGA CGCCCGCCGT GCTGCGCGAG ACCTATGGCG TGCTGCTGCG CGTGCTGTAT CCGGTCGTGC CGCACGTCAC GTACGAGCTG TGGAAGGCGC TCGGCTACGC GGACGAGTTT GGCCCGCTTC TCGACGCGCC GTGGCCGAAG GTCGACGAGG CCGCGCTCGA GCAGGCCGAG ATCGAGCTCG TGCTGCAGAT CAACGGCAAG GTGCGCGGCG CGCTGAAGGT GGCGAAGGAC GCGAGCCGCG AGGCGATCGA GGCGGCGGCC GTCGCCGACG AGATGTTCGC GAAATTCGCC GAAGGCAAGC CGGCGAAGAA GATCATTGTC GTGCCGGGCC GTCTCGTGAA CGTCGTCGTC TAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA