ButhA.17941.a

tRNA-specific 2-thiouridylase mnmA (EC 2.8.1.-) (BTH_I1257) (mnmA)

CENTER ID: ButhA.17941.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.17941.a.A1.GE32980 full length 1 386
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.4017
RefSeq: YP_441804.1
UniProt: Q2SZ45

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTKRRVVVGM SGGVDSSVTA WLLKEQGYDV IGLFMKNWED DDDGEYCSTR QDWIDVVSVA DLIGIDVEAV NFAAEYKDRV FAEFLREYSA GRTPNPDVLC NAEIKFKAFL DHAMSLGAET IATGHYARVR ERDGRFELLK AFDHTKDQSY FLHRLNQAQL SKTMFPLGEI PKTKVREIAA QIGLPNAKKK DSTGICFIGE RPFRDFLNRY LPTQPGPMKT PDGKTVGEHI GLAFYTFGQR KGIGLGGSKD GSGEPWFVAA KDIASNTLYV VQGHDHPWLR SRELVAGNVS WVAGEPPSDG ARCGAKTRYR QADAPCAFSS AAPGEAAGGE RFSLVFDEPQ WAVTPGQSAV LYDGDVCLGG GIIEAAATGR PDAAPASCES ALAEAR
NT Sequence
atgacgaagc gtcgagtcgt ggtgggcatg tcgggcggcg tcgattcgtc ggtgaccgcg tggctgctga aggaacaggg ttacgacgtc atcggcctct tcatgaagaa ctgggaggac gacgacgacg gcgaatactg ctcgacgcgg caggactgga tcgacgtcgt gtcggtcgcg gatctgatcg gcatcgacgt cgaggccgtc aacttcgccg ccgaatacaa ggaccgcgtg ttcgccgagt tcctgcgcga atactcggcg ggccgcacgc cgaacccgga cgtgctgtgc aacgcggaaa tcaagttcaa ggcgttcctc gatcacgcga tgtcgctcgg cgcggaaacg atcgcgaccg gccactacgc gcgcgtgcgc gagcgcgacg gccgcttcga gctgctgaag gcgttcgatc atacgaaaga ccagtcgtac ttcctgcatc gcctcaatca ggcgcagttg tcgaagacga tgttcccgct cggcgagatc ccgaagacga aggtgcgcga gatcgccgcg cagatcggcc tgccgaacgc gaagaagaag gattcgaccg gcatctgctt catcggcgag cgcccgttcc gcgatttcct gaaccgctat ctgccgacgc aacccggccc gatgaagacg cccgacggca agacggtcgg cgagcacatc ggcctcgcgt tctatacgtt cggccagagg aagggcatcg gcctcggcgg cagcaaggac ggcagcggcg agccgtggtt cgtcgcggcg aaggacatcg cgtcgaacac gctgtacgtc gtgcagggcc acgatcatcc gtggctgcgc tcgcgcgagc tcgtcgcggg caacgtgagc tgggtcgcgg gcgagccgcc ctccgacggc gcgcggtgcg gcgcgaaaac ccgctatcgg caggcggacg cgccgtgcgc gttcagcagc gccgcgcccg gcgaggcggc gggcggcgag cgcttctcgc tcgtgttcga cgagccgcaa tgggcggtca cgcccggcca gtcggcggtg ctgtacgacg gcgacgtgtg cctcggcggc gggatcatcg aagccgcggc gacgggacgc cccgacgccg cgcccgcgag ctgcgagagc gcgctcgccg aagcgcgctg a
Details for ButhA.17941.a.A1.GE32980
HARVESTED ON: 8/16/2011
SEQUENCED ON: 8/22/2011
EXPECTED MW: 44kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 86
PERCENT COVERAGE: 102
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTKRRVVVG MSGGVDSSVT AWLLKEQGYD VIGLFMKNWE DDDDGEYCST RQDWIDVVSV ADLIGIDVEA VNFAAEYKDR VFAEFLREYS AGRTPNPDVL CNAEIKFKAF LDHAMSLGAE TIATGHYARV RERDGRFELL KAFDHTKDQS YFLHRLNQAQ LSKTMFPLGE IPKTKVREIA AQIGLPNAKK KDSTGICFIG ERPFRDFLNR YLPTQPGPMK TPDGKTVGEH IGLAFYTFGQ RKGIGLGGSK DGSGEPWFVA AXDIASNTLY VVQGHDHPWL RSRELVAGNV SWVAGEPPSD GARXGXKXRY RQXXAPXAFS XAAPGXAXXE XFSLXXXXPQ WXVTPXXXXX XXXXXXXXXX XXXXXXXGXP XXXXXXXXXX XXXXXXXXXX XXXXXXXXX
Validated NT Sequence
ttttgtttac tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgacgaa gcgtcgagtc gtggtgggca tgtcgggcgg cgtcgattcg tcggtgaccg cgtggctgct gaaggaacag ggttacgacg tcatcggcct cttcatgaag aactgggagg acgacgacga cggcgaatac tgctcgacgc ggcaggactg gatcgacgtc gtgtcggtcg cggatctgat cggcatcgac gtcgaggccg tcaacttcgc cgccgaatac aaggaccgcg tgttcgccga gttcctgcgc gaatactcgg cgggccgcac gccgaacccg gacgtgctgt gcaacgcgga aatcaagttc aaggcgttcc tcgatcacgc gatgtcgctc ggcgcggaaa cgatcgcgac cggccactac gcgcgcgtgc gcgagcgcga cggccgcttc gagctgctga aggcgttcga tcatacgaaa gaccagtcgt acttcctgca tcgcctcaat caggcgcagt tgtcgaagac gatgttcccg ctcggcgaga tcccgaagac gaaggtgcgc gagatcgccg cgcagatcgg cctgccgaac gcgaagaaga aggattcgac cggcatctgc ttcatcggcg agcgcccgtt ccgcgatttc ctgaaccgct atctgccgac gcaacccggc ccgatgaaga cgcccgacgg caagacggtc ggcgagcaca tcggcctcgc gttctatacg ttcggccaga ggaagggcat cggcctcggc ggcagcaagg acggcagcgg cgagccgtgg ttcgtcgcgg cgaangacat cgcgtcgaac acgctgtacg tcgtgcaggg ccacgatcat ccgtggctgc gctcgcgcga gctcgtcgcg ggcaacgtga gctgggtcgc gggcgagccg ccctccgacg gcgcgcggtg nggcncgaaa ncccgctatc ggcaggngga ngcgccgngn gcgttcagca ncgccgcgcc cggcgangcg gnggnngagn gcttctcgct cnngntcnac nanccncaat ggnnggtcac gcccngngnn gnnnnctnnn cnnngncnnn nnnnngccnn gnnnncnggn nnnntnnnnn nngnnnnnnn ggnnnnccnn nnnccnnnnn cnnnnnnntn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncnn nnnncnnnnn nnnnnnnnna nnncnnna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTKRRVVVG MSGGVDSSVT AWLLKEQGYD VIGLFMKNWE DDDDGEYCST RQDWIDVVSV ADLIGIDVEA VNFAAEYKDR VFAEFLREYS AGRTPNPDVL CNAEIKFKAF LDHAMSLGAE TIATGHYARV RERDGRFELL KAFDHTKDQS YFLHRLNQAQ LSKTMFPLGE IPKTKVREIA AQIGLPNAKK KDSTGICFIG ERPFRDFLNR YLPTQPGPMK TPDGKTVGEH IGLAFYTFGQ RKGIGLGGSK DGSGEPWFVA AKDIASNTLY VVQGHDHPWL RSRELVAGNV SWVAGEPPSD GARCGAKTRY RQADAPCAFS SAAPGEAAGG ERFSLVFDEP QWAVTPGQSA VLYDGDVCLG GGIIEAAATG RPDAAPASCE SALAEAR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacgaagcgt cgagtcgtgg tgggcatgtc gggcggcgtc gattcgtcgg tgaccgcgtg gctgctgaag gaacagggtt acgacgtcat cggcctcttc atgaagaact gggaggacga cgacgacggc gaatactgct cgacgcggca ggactggatc gacgtcgtgt cggtcgcgga tctgatcggc atcgacgtcg aggccgtcaa cttcgccgcc gaatacaagg accgcgtgtt cgccgagttc ctgcgcgaat actcggcggg ccgcacgccg aacccggacg tgctgtgcaa cgcggaaatc aagttcaagg cgttcctcga tcacgcgatg tcgctcggcg cggaaacgat cgcgaccggc cactacgcgc gcgtgcgcga gcgcgacggc cgcttcgagc tgctgaaggc gttcgatcat acgaaagacc agtcgtactt cctgcatcgc ctcaatcagg cgcagttgtc gaagacgatg ttcccgctcg gcgagatccc gaagacgaag gtgcgcgaga tcgccgcgca gatcggcctg ccgaacgcga agaagaagga ttcgaccggc atctgcttca tcggcgagcg cccgttccgc gatttcctga accgctatct gccgacgcaa cccggcccga tgaagacgcc cgacggcaag acggtcggcg agcacatcgg cctcgcgttc tatacgttcg gccagaggaa gggcatcggc ctcggcggca gcaaggacgg cagcggcgag ccgtggttcg tcgcggcgaa ggacatcgcg tcgaacacgc tgtacgtcgt gcagggccac gatcatccgt ggctgcgctc gcgcgagctc gtcgcgggca acgtgagctg ggtcgcgggc gagccgccct ccgacggcgc gcggtgcggc gcgaaaaccc gctatcggca ggcggacgcg ccgtgcgcgt tcagcagcgc cgcgcccggc gaggcggcgg gcggcgagcg cttctcgctc gtgttcgacg agccgcaatg ggcggtcacg cccggccagt cggcggtgct gtacgacggc gacgtgtgcc tcggcggcgg gatcatcgaa gccgcggcga cgggacgccc cgacgccgcg cccgcgagct gcgagagcgc gctcgccgaa gcgcgctaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa