ButhA.17936.a

Fructose-1,6-bisphosphatase class 1 (FBPase class 1) (EC 3.1.3.11) (D-fructose-1,6-bisphosphate 1-phosphohydrolase class 1) (BTH_I1604) (fbp)

CENTER ID: ButhA.17936.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.17936.a.A1.GE32940 full length 1 338
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.4389
RefSeq: YP_442142.1
UniProt: Q2SY57

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSITRRTTLS KYLIEQQRET HNLPADLRLL IEVVARACKA ISYNVSKGAL GDALGTAGSE NVQGEVQKKL DILSNEILLD ANEWGGNLAA MASEEMETFF PIPANYPRGE YLLVFDPLDG SSNIDVNVSI GTIFSVLRCP DGQQATEQSF LQPGTEQVAA GYAVYGPQTV FVLTTGNGVN CFTLDREVGS WVLTQSNLRI PEDTREYAIN ASNARHWYDP VKRYVDELNA GAEGPRGENF NMRWIASMVA DVHRILNRGG IFMYPADKRT PDRPGKLRLM YEANPMSFIV EQAGGAATTG LKRILDVQPT GLHQRVPVIL GSKNEVERVA RYHQEAQS
NT Sequence
atgtccatta cccgacgcac cacgctgtcc aagtatctga tcgagcagca gcgtgagacc cacaacctcc ccgccgacct gcgcctgttg atcgaagtgg tcgcgcgcgc gtgcaaggcg atcagctaca acgtgagcaa gggcgcgttg ggcgatgcgc tcggcacggc gggcagcgag aatgtccagg gcgaagtgca gaagaagctc gacatcctgt cgaacgagat cctgctcgac gcgaacgaat ggggcggcaa tctcgccgcg atggcgtcgg aggaaatgga gacgttcttc ccgatcccgg cgaactaccc gcgcggcgaa tacctgctcg tgttcgatcc gctcgacggc tcgtcgaaca tcgacgtgaa cgtgtcgatc ggcacgatct tctcggtgct gcgctgcccg gacggccagc aggcgaccga gcaatcgttc ctgcagccgg gcaccgagca ggtcgcggcg ggttacgcgg tgtacggccc gcaaaccgtg ttcgtgctga cgacgggcaa cggcgtgaac tgcttcacgc tcgaccgcga agtcggctcg tgggtgctca cgcaaagcaa cctgcggatt ccggaggaca cgcgcgagta tgcgatcaac gcgtcgaacg cgcgccactg gtacgacccg gtgaagcgct acgtcgacga gctgaacgcg ggcgccgaag gcccgcgcgg cgagaacttc aacatgcgct ggatcgcctc gatggtcgcc gacgtgcaca ggatcctgaa ccgcggcggc atcttcatgt acccggccga caagcgcacg ccggatcgtc cgggcaagct gcgcctgatg tacgaagcga acccgatgtc gttcatcgtc gagcaggcgg gcggcgcggc gacgacgggc ctgaagcgga tcctcgacgt gcagccgacg ggcctgcacc agcgcgtgcc ggtgatcctc ggctcgaaga acgaagtcga gcgcgtcgcg cgctatcacc aggaagcgca gtcttaa
Details for ButhA.17936.a.A1.GE32940
HARVESTED ON: 7/12/2011
SEQUENCED ON: 7/28/2011
EXPECTED MW: 39kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 94
PERCENT COVERAGE: 76
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSITRRTTL SKYLIEQQRE THNLPADLRL LIEVVARACK AISYNVSKGA LGDALGTAGS ENVQGEVQKK LDILSNEILL DANEWGGNLA AMASEEMETF FPIPANYPRG EYLLVFDPLD GSSNIDVNVS IGTIFSVLRC PDGQQATEQS FLQPGTEQVA AGYAVYGPQT VFVLTTGNGV NCFTLDREVG SWVLTQSNLR IPEDTREYAI NASNARHWYD PVKRYVDELN AGAXXRAART STCAGSPRWS PTCTGS
Validated NT Sequence
ttttgttnnn tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgtccat tacccgacgc accacgctgt ccaagtatct gatcgagcag cagcgtgaga cccacaacct ccccgccgac ctgcgcctgt tgatcgaagt ggtcgcgcgc gcgtgcaagg cgatcagcta caacgtgagc aagggcgcgt tgggcgatgc gctcggcacg gcgggcagcg agaatgtcca gggcgaagtg cagaagaagc tcgacatcct gtcgaacgag atcctgctcg acgcgaacga atggggcggc aatctcgccg cgatggcgtc ggaggaaatg gagacgttct tcccgatccc ggcgaactac ccgcgcggcg aatacctgct cgtgttcgat ccgctcgacg gctcgtcgaa catcgacgtg aacgtgtcga tcggcacgat cttctcggtg ctgcgctgcc cggacggcca gcaggcgacc gagcaatcgt tcctgcagcc gggcaccgag caggtcgcgg cgggttacgc ggtgtacggc ccgcaaaccg tgttcgtgct gacgacgggc aacggcgtga actgcttcac gctcgaccgc gaagtcggct cgtgggtgct cacgcaaagc aacctgcgga ttccggagga cacgcgcgag tatgcgatca acgcgtcgaa cgcgcgccac tggtacgacc cggtgaagcg ctacgtcgac gagctgaacg cgggcgccna nnnccgcgcg gcgagaactt caacatgcgc tggatcgcct cgatggtcgc cgacgtgcac aggatcctga ancgcggcgg cntcttcatg tacccggccg acaagcgcac gccggatcgt ccgggcaagc tgcgcctgat gtacnaagcn nacccnatgt cgttcatcgt cnagcaggcg ggcggcgcgc nacnanngnc ctgaagcgna tcntcnacgt gcanccgacg gnnngcacca gcgcnnnnng gtgatcntnn gctcnnnann annnnnnnnn ntcnnnnncn nncnccnnnn nnnnanncnt nnnnannnnn nnnannnnnn nnncannnnn nnnnngnnnn nnnnnnnnnn nnnnnnnnnn nnnnaannan nnnngnnngn ng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSITRRTTL SKYLIEQQRE THNLPADLRL LIEVVARACK AISYNVSKGA LGDALGTAGS ENVQGEVQKK LDILSNEILL DANEWGGNLA AMASEEMETF FPIPANYPRG EYLLVFDPLD GSSNIDVNVS IGTIFSVLRC PDGQQATEQS FLQPGTEQVA AGYAVYGPQT VFVLTTGNGV NCFTLDREVG SWVLTQSNLR IPEDTREYAI NASNARHWYD PVKRYVDELN AGAEGPRGEN FNMRWIASMV ADVHRILNRG GIFMYPADKR TPDRPGKLRL MYEANPMSFI VEQAGGAATT GLKRILDVQP TGLHQRVPVI LGSKNEVERV ARYHQEAQS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtccattacc cgacgcacca cgctgtccaa gtatctgatc gagcagcagc gtgagaccca caacctcccc gccgacctgc gcctgttgat cgaagtggtc gcgcgcgcgt gcaaggcgat cagctacaac gtgagcaagg gcgcgttggg cgatgcgctc ggcacggcgg gcagcgagaa tgtccagggc gaagtgcaga agaagctcga catcctgtcg aacgagatcc tgctcgacgc gaacgaatgg ggcggcaatc tcgccgcgat ggcgtcggag gaaatggaga cgttcttccc gatcccggcg aactacccgc gcggcgaata cctgctcgtg ttcgatccgc tcgacggctc gtcgaacatc gacgtgaacg tgtcgatcgg cacgatcttc tcggtgctgc gctgcccgga cggccagcag gcgaccgagc aatcgttcct gcagccgggc accgagcagg tcgcggcggg ttacgcggtg tacggcccgc aaaccgtgtt cgtgctgacg acgggcaacg gcgtgaactg cttcacgctc gaccgcgaag tcggctcgtg ggtgctcacg caaagcaacc tgcggattcc ggaggacacg cgcgagtatg cgatcaacgc gtcgaacgcg cgccactggt acgacccggt gaagcgctac gtcgacgagc tgaacgcggg cgccgaaggc ccgcgcggcg agaacttcaa catgcgctgg atcgcctcga tggtcgccga cgtgcacagg atcctgaacc gcggcggcat cttcatgtac ccggccgaca agcgcacgcc ggatcgtccg ggcaagctgc gcctgatgta cgaagcgaac ccgatgtcgt tcatcgtcga gcaggcgggc ggcgcggcga cgacgggcct gaagcggatc ctcgacgtgc agccgacggg cctgcaccag cgcgtgccgg tgatcctcgg ctcgaagaac gaagtcgagc gcgtcgcgcg ctatcaccag gaagcgcagt cttaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa