SSGCID: Target Page

ButhA.17935.a

60 kDa chaperonin 1 (GroEL protein 1) (Protein Cpn60 1) (BTH_I1458) (groL1)

CENTER ID:	ButhA.17935.a
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.17935.a.A1.GE34814	full length		1	546

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.17935.a.A1.PW37275	full length		1	546

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.4239
RefSeq:	YP_442004.1
UniProt:	Q2SYJ5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAAKDVVFGD SARAKMVEGV NILANAVKVT LGPKGRNVVL ERSFGGPTVT KDGVSVAKEI ELKDKLQNMG AQMVKEVASK TSDNAGDGTT TATVLAQSIV REGMKYVASG MNPMDLKRGI DKAVAAAVEE LKKISKPCTT NKEIAQVGSI SANSDSSIGD RIAEAMDKVG KEGVITVEDG KSLADELDVV EGMQFDRGYL SPYFINNPDK QVAVLENPFV LLHDKKVSNI RDLLPVLEQV AKAGRPLLII AEDVEGEALA TLVVNNIRGI LKTVAVKAPG FGDRRKAMLE DIAILTGGQV IAEETGLTLE KATLAELGQA KRIEVGKENT TIIDGAGEAV NIEARVKQIR TQIEEATSDY DREKLQERVA KLAGGVAVIK VGAATEVEMK EKKARVEDAL HATRAAVEEG IVPGGGVALI RARTAIAALT GVNADQNAGI KIVLRAMEEP LRQIVTNGGE EASVVVAAVA AGKGNYGYNA ATGEYVDMVE AGVVDPTKVT RTALQNAASV AGLLLTTDAA VAELPKEDAP MPGGMPGGMG GMGMDM

NT Sequence

atggcagcta aagacgtcgt attcggcgat tccgcacgcg cgaagatggt cgaaggcgtg aacattctcg ccaacgctgt gaaggtcacg ctgggtccga agggccgcaa cgtggtgctc gagcgcagct tcggcggccc gacggtcacc aaggacggtg tgtcggtcgc gaaggaaatc gagctgaagg acaagctcca gaacatgggt gcgcaaatgg tcaaggaagt cgcttccaag accagcgaca acgcaggcga cggcacgacg acggcaaccg tcctcgcgca atcgatcgtc cgcgaaggca tgaagtacgt cgcatcgggc atgaacccga tggacctgaa gcgcggcatc gacaaggcag tcgcagcggc tgtcgaagag ctgaagaaga tcagcaagcc gtgcacgacg aacaaggaaa tcgcacaggt cggctcgatc tcggcgaaca gcgattcgtc gatcggcgat cgcatcgctg aagcgatgga caaggtcggc aaggaaggcg tgatcaccgt cgaagacggc aagtcgctcg ccgacgagct cgacgtcgtc gaaggcatgc aattcgaccg cggctacctg tcgccgtact tcatcaacaa cccggacaag caagtcgccg tcctcgagaa cccgttcgtg ctgctgcacg acaagaaggt gtcgaacatc cgcgatctgc tgccggtgct cgagcaagtc gcgaaggctg gccgtccgct gctcatcatc gctgaagacg tcgaaggcga agcgctcgcg acgctggtcg tcaacaacat ccgcggcatc ctgaagaccg ttgcggtcaa ggcgccgggc ttcggcgacc gtcgcaaggc gatgctggaa gacatcgcga tcctgacggg cggccaagtc attgcggaag aaaccggcct cacgctcgag aaggcaacgc tggcagaact gggccaggcg aagcgcatcg aagtgggcaa ggaaaacacg acgatcatcg acggcgctgg cgaagccgtg aacatcgaag cgcgcgtcaa gcaaatccgc acgcagatcg aagaagcgac gtcggactac gaccgtgaaa agctgcaaga gcgcgtggcc aagctggccg gcggtgttgc agtgatcaag gtcggcgcgg cgaccgaagt cgaaatgaag gaaaagaagg cacgtgtcga agacgcgctg cacgcaaccc gcgcggccgt tgaagaaggc atcgtcccgg gcggcggtgt cgcgctgatc cgcgcacgca ccgcaatcgc tgcactgacc ggcgtgaacg ccgaccagaa cgccggcatc aagatcgtgc tgcgcgcaat ggaagagccg ctgcgccaga tcgtcacgaa cggcggcgaa gaagcgagcg tcgtggtggc ggcagttgct gcaggcaagg gcaactacgg ctacaacgcg gcgacgggcg agtacgtcga catggttgag gccggcgtcg tcgatccgac gaaggtcacg cgcaccgcgc tgcagaacgc ggcttcggtc gcaggcctgc tgctgacgac ggacgcagcc gtcgccgaac tgccgaagga agacgctccg atgccgggcg gcatgccggg cggcatgggc ggcatgggca tggacatgta a

Details for ButhA.17935.a.A1.GE34814

HARVESTED ON:	3/13/2012
SEQUENCED ON:	3/12/2012
EXPECTED MW:	59kDa
OBSERVED MW:	64kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	98
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMAAKDVVFG DSARAKMVEG VNILANAVKV TLGPKGRNVV LERSFGGPTV TKDGVSVAKE IELKDKLQNM GAQMVKEVAS KTSDNAGDGT TTATVLAQSI VREGMKYVAS GMNPMDLKRG IDKAVAAAVE ELKKISKPCT TNKEIAQVGS ISANSDSSIG DRIAEAMDKV GKEGVITVED GKSLAXXLDV VEXMQFDRGY LSPYFINNPD KQVAVLENPF VLLHDKKVSN IRDLLPVLEQ VAKXGRPLLI IAEDVEXEAL ATLVVNNIRG XXKTVAVKAP GFGDRRKAML EDIAILTGGQ VIAEETGLTL EKATLAELGQ AKRIEVGKEN TTIIDGAGEA VNIEARVKQI RTQIEEATSD YDREKLQERV AKLAGGVAVI KVGAATEVEM KEKKARVEDA LHATRAAVEE GIVPGGGVAL IRARTAIAAL TGVNADQNAG IKIVLRAMEE PLRQIVTNGG EEASVVVAAV AAGKGNYGYN AATGEYVDMV EAGVVDPTKV TRTALQNAAS VAGLLLTTDA AVAELPKEDA PMPGGMPGGM GGMGM

Validated NT Sequence

ccatgcccat gccgcccatg ccgcccggca tgccgcccgg catcggagcg tcttccttcg gcagttcggc gacggctgcg tccgtcgtca gcagcaggcc tgcgaccgaa gccgcgttct gcagcgcggt gcgcgtgacc ttcgtcggat cgacgacgcc ggcctcaacc atgtcgacgt actcgcccgt cgccgcgttg tagccgtagt tgcccttgcc tgcagcaact gccgccacca cgacgctcgc ttcttcgccg ccgttcgtga cgatctggcg cagcggctct tccattgcgc gcagcacgat cttgatgccg gcgttctggt cggcgttcac gccggtcagt gcagcgattg cggtgcgtgc gcggatcagc gcgacaccgc cgcccgggac gatgccttct tcaacggccg cgcgggttgc gtgcagcgcg tcttcgacac gtgccttctt ttccttcatt tcgacttcgg tcgccgcgcc gaccttgatc actgcaacac cgccggccag cttggccacg cgctcttgca gcttttcacg gtcgtagtcc gacgtcgctt cttcgatctg cgtgcggatt tgcttgacgc gcgcttcgat gttcacggct tcgccagcgc cgtcgatgat cgtcgtgttt tccttgccca cttcgatgcg cttcgcctgg cccagttctg ccagcgttgc cttctcgagc gtgaggccgg tttcttccgc aatgacttgg ccgcccgtca ggatcgcgat gtcttccagc atcgccttgc gacggtcgcc gaagcccggc gccttgaccg caacggtctt cnnnatgccg cggatgttgt tgacgaccag cgtcgcgagc gcttcgnctt cnacgtcttc agcgatgatg agcagcggac ggccancctt cgcgacttgc tcgagcaccg gcagcagatc gcggatgttn gacaccttct tgtcgtgcag cagcacgaac gggttctcga ggacggcgac ttgcttgtcc gggttgttga tgaagtacgg cgacaggtag ccgcggtcga attgcatgcn ttcgacgacg tcgagctnnt nggcgagcga cttgccgtct tcgacggtga tcacgccttc cttgccgacc ttgtccatcg cttcagcgat gcgatcgccg atcgacgaat cgctgttcgc cgagatcgag ccgacctgtg cgatttcctt gttcgtcgtg cacggcttgc tgatcttctt cagctcttcg acagccgctg cgactgcctt gtcgatgccg cgcttcaggt ccatcgggtt catgcccgat gcgacgtact tcatgccttc gcggacgatc gattgcgcga ggacggttgc cgtcgtcgtg ccgtcgcctg cgttgtcgct ggtcttggaa gcgacttcct tgaccatttg cgcacccatg ttctggagct tgtccttcag ctcgatttcc ttcgcgaccg acacaccgtc cttggtgacc gtcgggccgc cgaagctgcg ctcgagcacc acgttgcggc ccttcggacc cagcgtgacc ttcacagcgt tggcgagaat gttcacgcct tcgaccatct tcgcgcgtgc ggaatcgccg aatacgacgt ctttagctgc catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccat

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMAAKDVVFG DSARAKMVEG VNILANAVKV TLGPKGRNVV LERSFGGPTV TKDGVSVAKE IELKDKLQNM GAQMVKEVAS KTSDNAGDGT TTATVLAQSI VREGMKYVAS GMNPMDLKRG IDKAVAAAVE ELKKISKPCT TNKEIAQVGS ISANSDSSIG DRIAEAMDKV GKEGVITVED GKSLADELDV VEGMQFDRGY LSPYFINNPD KQVAVLENPF VLLHDKKVSN IRDLLPVLEQ VAKAGRPLLI IAEDVEGEAL ATLVVNNIRG ILKTVAVKAP GFGDRRKAML EDIAILTGGQ VIAEETGLTL EKATLAELGQ AKRIEVGKEN TTIIDGAGEA VNIEARVKQI RTQIEEATSD YDREKLQERV AKLAGGVAVI KVGAATEVEM KEKKARVEDA LHATRAAVEE GIVPGGGVAL IRARTAIAAL TGVNADQNAG IKIVLRAMEE PLRQIVTNGG EEASVVVAAV AAGKGNYGYN AATGEYVDMV EAGVVDPTKV TRTALQNAAS VAGLLLTTDA AVAELPKEDA PMPGGMPGGM GGMGMDM

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcagctaaa gacgtcgtat tcggcgattc cgcacgcgcg aagatggtcg aaggcgtgaa cattctcgcc aacgctgtga aggtcacgct gggtccgaag ggccgcaacg tggtgctcga gcgcagcttc ggcggcccga cggtcaccaa ggacggtgtg tcggtcgcga aggaaatcga gctgaaggac aagctccaga acatgggtgc gcaaatggtc aaggaagtcg cttccaagac cagcgacaac gcaggcgacg gcacgacgac ggcaaccgtc ctcgcgcaat cgatcgtccg cgaaggcatg aagtacgtcg catcgggcat gaacccgatg gacctgaagc gcggcatcga caaggcagtc gcagcggctg tcgaagagct gaagaagatc agcaagccgt gcacgacgaa caaggaaatc gcacaggtcg gctcgatctc ggcgaacagc gattcgtcga tcggcgatcg catcgctgaa gcgatggaca aggtcggcaa ggaaggcgtg atcaccgtcg aagacggcaa gtcgctcgcc gacgagctcg acgtcgtcga aggcatgcaa ttcgaccgcg gctacctgtc gccgtacttc atcaacaacc cggacaagca agtcgccgtc ctcgagaacc cgttcgtgct gctgcacgac aagaaggtgt cgaacatccg cgatctgctg ccggtgctcg agcaagtcgc gaaggctggc cgtccgctgc tcatcatcgc tgaagacgtc gaaggcgaag cgctcgcgac gctggtcgtc aacaacatcc gcggcatcct gaagaccgtt gcggtcaagg cgccgggctt cggcgaccgt cgcaaggcga tgctggaaga catcgcgatc ctgacgggcg gccaagtcat tgcggaagaa accggcctca cgctcgagaa ggcaacgctg gcagaactgg gccaggcgaa gcgcatcgaa gtgggcaagg aaaacacgac gatcatcgac ggcgctggcg aagccgtgaa catcgaagcg cgcgtcaagc aaatccgcac gcagatcgaa gaagcgacgt cggactacga ccgtgaaaag ctgcaagagc gcgtggccaa gctggccggc ggtgttgcag tgatcaaggt cggcgcggcg accgaagtcg aaatgaagga aaagaaggca cgtgtcgaag acgcgctgca cgcaacccgc gcggccgttg aagaaggcat cgtcccgggc ggcggtgtcg cgctgatccg cgcacgcacc gcaatcgctg cactgaccgg cgtgaacgcc gaccagaacg ccggcatcaa gatcgtgctg cgcgcaatgg aagagccgct gcgccagatc gtcacgaacg gcggcgaaga agcgagcgtc gtggtggcgg cagttgctgc aggcaagggc aactacggct acaacgcggc gacgggcgag tacgtcgaca tggttgaggc cggcgtcgtc gatccgacga aggtcacgcg caccgcgctg cagaacgcgg cttcggtcgc aggcctgctg ctgacgacgg acgcagccgt cgccgaactg ccgaaggaag acgctccgat gccgggcggc atgccgggcg gcatgggcgg catgggcatg gacatgtaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa

Details for ButhA.17935.a.A1.PW37275

PURIFICATION DATe:	4/3/2013
CONCENTRATION:	32.6mg/ml
OBSERVED MW:	65kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	5
VIAL VOLUME:	110µl
PERCENT IDENTITY:	98
PERCENT COVERAGE:	99

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMAAKDVVFG DSARAKMVEG VNILANAVKV TLGPKGRNVV LERSFGGPTV TKDGVSVAKE IELKDKLQNM GAQMVKEVAS KTSDNAGDGT TTATVLAQSI VREGMKYVAS GMNPMDLKRG IDKAVAAAVE ELKKISKPCT TNKEIAQVGS ISANSDSSIG DRIAEAMDKV GKEGVITVED GKSLADELDV VEGMQFDRGY LSPYFINNPD KQVAVLENPF VLLHDKKVSN IRDLLPVLEQ VAKAGRPLLI IAEDVEGEAL ATLVVNNIRG ILKTVAVKAP GFGDRRKAML EDIAILTGGQ VIAEETGLTL EKATLAELGQ AKRIEVGKEN TTIIDGAGEA VNIEARVKQI RTQIEEATSD YDREKLQERV AKLAGGVAVI KVGAATEVEM KEKKARVEDA LHATRAAVEE GIVPGGGVAL IRARTAIAAL TGVNADQNAG IKIVLRAMEE PLRQIVTNGG EEASVVVAAV AAGKGNYGYN AATGEYVDMV EAGVVDPTKV TRTALQNAAS VAGLLLTTDA AVAELPKEDA PMPGGMPGGM GGMGMDM

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCAGCTAAA GACGTCGTAT TCGGCGATTC CGCACGCGCG AAGATGGTCG AAGGCGTGAA CATTCTCGCC AACGCTGTGA AGGTCACGCT GGGTCCGAAG GGCCGCAACG TGGTGCTCGA GCGCAGCTTC GGCGGCCCGA CGGTCACCAA GGACGGTGTG TCGGTCGCGA AGGAAATCGA GCTGAAGGAC AAGCTCCAGA ACATGGGTGC GCAAATGGTC AAGGAAGTCG CTTCCAAGAC CAGCGACAAC GCAGGCGACG GCACGACGAC GGCAACCGTC CTCGCGCAAT CGATCGTCCG CGAAGGCATG AAGTACGTCG CATCGGGCAT GAACCCGATG GACCTGAAGC GCGGCATCGA CAAGGCAGTC GCAGCGGCTG TCGAAGAGCT GAAGAAGATC AGCAAGCCGT GCACGACGAA CAAGGAAATC GCACAGGTCG GCTCGATCTC GGCGAACAGC GATTCGTCGA TCGGCGATCG CATCGCTGAA GCGATGGACA AGGTCGGCAA GGAAGGCGTG ATCACCGTCG AAGACGGCAA GTCGCTCGCC GACGAGCTCG ACGTCGTCGA AGGCATGCAA TTCGACCGCG GCTACCTGTC GCCGTACTTC ATCAACAACC CGGACAAGCA AGTCGCCGTC CTCGAGAACC CGTTCGTGCT GCTGCACGAC AAGAAGGTGT CGAACATCCG CGATCTGCTG CCGGTGCTCG AGCAAGTCGC GAAGGCTGGC CGTCCGCTGC TCATCATCGC TGAAGACGTC GAAGGCGAAG CGCTCGCGAC GCTGGTCGTC AACAACATCC GCGGCATCCT GAAGACCGTT GCGGTCAAGG CGCCGGGCTT CGGCGACCGT CGCAAGGCGA TGCTGGAAGA CATCGCGATC CTGACGGGCG GCCAAGTCAT TGCGGAAGAA ACCGGCCTCA CGCTCGAGAA GGCAACGCTG GCAGAACTGG GCCAGGCGAA GCGCATCGAA GTGGGCAAGG AAAACACGAC GATCATCGAC GGCGCTGGCG AAGCCGTGAA CATCGAAGCG CGCGTCAAGC AAATCCGCAC GCAGATCGAA GAAGCGACGT CGGACTACGA CCGTGAAAAG CTGCAAGAGC GCGTGGCCAA GCTGGCCGGC GGTGTTGCAG TGATCAAGGT CGGCGCGGCG ACCGAAGTCG AAATGAAGGA AAAGAAGGCA CGTGTCGAAG ACGCGCTGCA CGCAACCCGC GCGGCCGTTG AAGAAGGCAT CGTCCCGGGC GGCGGTGTCG CGCTGATCCG CGCACGCACC GCAATCGCTG CACTGACCGG CGTGAACGCC GACCAGAACG CCGGCATCAA GATCGTGCTG CGCGCAATGG AAGAGCCGCT GCGCCAGATC GTCACGAACG GCGGCGAAGA AGCGAGCGTC GTGGTGGCGG CAGTTGCTGC AGGCAAGGGC AACTACGGCT ACAACGCGGC GACGGGCGAG TACGTCGACA TGGTTGAGGC CGGCGTCGTC GATCCGACGA AGGTCACGCG CACCGCGCTG CAGAACGCGG CTTCGGTCGC AGGCCTGCTG CTGACGACGG ACGCAGCCGT CGCCGAACTG CCGAAGGAAG ACGCTCCGAT GCCGGGCGGC ATGCCGGGCG GCATGGGCGG CATGGGCATG GACATGTAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA