SSGCID: Target Page

ButhA.17927.a

UDP-2,3-diacylglucosamine hydrolase (EC 3.6.1.-) (BTH_I1937) (lpxH)

CENTER ID:	ButhA.17927.a
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.17927.a.A1.GE34774	full length		1	266

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.4744
RefSeq:	YP_442467.1
UniProt:	Q2SX82

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLQETPPRSV FAGVPGERGL AHAARPFLFV SDLHLSDAIP HTVAAFEHFV RVTADSADSV FILGDLFEYW IGDDILDHDP FAARMAALLH TFSERGIALY VMRGNRDFLL GKRFMKAAGA LLIPDPSVVV AFGNRIVLAH GDAQCTADRG YQWFRRFARN GIAQRLFLAW PFAWRRALAQ RMRASSEAGR MRPASPRYDV TRKGVAALFK RSGAHTIVHG HTHKPARHVE AGGVRWVLPD WELDHGPRRG GYLRVDAEGI RALPLD

NT Sequence

atgctgcagg aaacgccgcc gcgaagcgtg tttgcgggcg tgccgggcga gcgcgggctc gcgcacgcgg cgcgcccgtt cctgttcgtc tccgatctgc acctgagcga cgcgatcccg cacacggtcg ccgcgttcga gcacttcgtg cgcgtgacgg ccgacagcgc ggattcggtg ttcatcctcg gcgatctgtt cgaatactgg atcggcgacg acatcctcga ccacgatccg ttcgccgcac ggatggcggc cttgctgcac acgttctccg agcgcggcat cgcgctctac gtgatgcgcg gcaaccgcga cttcctgctc ggcaagcgct tcatgaaggc ggcgggcgcg ctcctcattc cggacccgtc cgtcgtcgtc gcgttcggca accgcatcgt gctcgcgcat ggcgatgcgc aatgcacggc cgaccgcggc taccagtggt ttcgccgctt cgcgcgcaac ggcatcgcgc agcggctctt tctcgcttgg ccgttcgcgt ggcgacgcgc gctcgcgcaa cggatgcgcg catcgagcga agccggccgg atgcgccccg cttcgccgcg ctatgacgtg acgcgcaagg gcgtcgccgc gctcttcaag cgcagcggtg cgcacacgat cgttcacggc cacacgcaca aaccggcgcg gcatgtcgag gcgggcggcg tgcgctgggt gttgccggat tgggagctcg accacgggcc gcgacgcggc ggctatctgc gcgtcgacgc ggaagggata cgtgcgctgc cgctcgattg a

Details for ButhA.17927.a.A1.GE34774

HARVESTED ON:	3/13/2012
SEQUENCED ON:	3/12/2012
EXPECTED MW:	31kDa
OBSERVED MW:	31kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMLQETPPRS VFAGVPGERG LAHAARPFLF VSDLHLSDAI PHTVAAFEHF VRVTADSADS VFILGDLFEY WIGDDILDHD PFAARMAALL HTFSERGIAL YVMRGNRDFL LGKRFMKAAG ALLIPDPSVV VAFGNRIVLA HGDAQCTADR GYQWFRRFAR NGIAQRLFLA WPLAQRMRAS SEAGRMRPAS PRYDVTRKGV AALFKRSGAH TIVHGHTHKP ARHVEAGGVR WVLPDWELDH GPRRGGYLRV DAEGIRALPL D

Validated NT Sequence

ttattaatcg agcggcagcg cacgtatccc ttccgcgtcg acgcgcagat agccgccgcg tcgcggcccg tggtcgagct cccaatccgg caacacccag cgcacgccgc ccgcctcgac atgccgcgcc ggtttgtgcg tgtggccgtg aacgatcgtg tgcgcaccgc tgcgcttgaa gagcgcggcg acgcccttgc gcgtcacgtc atagcgcggc gaagcggggc gcatccggcc ggcttcgctc gatgcgcgca tccgttgcgc gagcggccaa gcgagaaaga gccgctgcgc gatgccgttg cgcgcgaagc ggcgaaacca ctggtagccg cggtcggccg tgcattgcgc atcgccatgc gcgagcacga tgcggttgcc gaacgcgacg acgacggacg ggtccggaat gaggagcgcg cccgccgcct tcatgaagcg cttgccgagc aggaagtcgc ggttgccgcg catcacgtag agcgcgatgc cgcgctcgga gaacgtgtgc agcaaggccg ccatccgtgc ggcgaacgga tcgtggtcga ggatgtcgtc gccgatccag tattcgaaca gatcgccgag gatgaacacc gaatccgcgc tgtcggccgt cacgcgcacg aagtgctcga acgcggcgac cgtgtgcggg atcgcgtcgc tcaggtgcag atcggagacg aacaggaacg ggcgcgccgc gtgcgcgagc ccgcgctcgc ccggcacgcc cgcaaacacg cttcgcggcg gcgtttcctg cagcatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccatg gtat

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMLQETPPRS VFAGVPGERG LAHAARPFLF VSDLHLSDAI PHTVAAFEHF VRVTADSADS VFILGDLFEY WIGDDILDHD PFAARMAALL HTFSERGIAL YVMRGNRDFL LGKRFMKAAG ALLIPDPSVV VAFGNRIVLA HGDAQCTADR GYQWFRRFAR NGIAQRLFLA WPFAWRRALA QRMRASSEAG RMRPASPRYD VTRKGVAALF KRSGAHTIVH GHTHKPARHV EAGGVRWVLP DWELDHGPRR GGYLRVDAEG IRALPLD

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gctgcaggaa acgccgccgc gaagcgtgtt tgcgggcgtg ccgggcgagc gcgggctcgc gcacgcggcg cgcccgttcc tgttcgtctc cgatctgcac ctgagcgacg cgatcccgca cacggtcgcc gcgttcgagc acttcgtgcg cgtgacggcc gacagcgcgg attcggtgtt catcctcggc gatctgttcg aatactggat cggcgacgac atcctcgacc acgatccgtt cgccgcacgg atggcggcct tgctgcacac gttctccgag cgcggcatcg cgctctacgt gatgcgcggc aaccgcgact tcctgctcgg caagcgcttc atgaaggcgg cgggcgcgct cctcattccg gacccgtccg tcgtcgtcgc gttcggcaac cgcatcgtgc tcgcgcatgg cgatgcgcaa tgcacggccg accgcggcta ccagtggttt cgccgcttcg cgcgcaacgg catcgcgcag cggctctttc tcgcttggcc gttcgcgtgg cgacgcgcgc tcgcgcaacg gatgcgcgca tcgagcgaag ccggccggat gcgccccgct tcgccgcgct atgacgtgac gcgcaagggc gtcgccgcgc tcttcaagcg cagcggtgcg cacacgatcg ttcacggcca cacgcacaaa ccggcgcggc atgtcgaggc gggcggcgtg cgctgggtgt tgccggattg ggagctcgac cacgggccgc gacgcggcgg ctatctgcgc gtcgacgcgg aagggatacg tgcgctgccg ctcgattaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa