ButhA.17926.a

Glucosamine--fructose-6-phosphate aminotransferase, isomerizing (EC 2.6.1.16) (BTH_I0288) (glmS-1)

CENTER ID: ButhA.17926.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.17926.a.A1.GE32930 full length 1 610
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.17926.a.A1.PS01193 full length 1 610

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.2948
RefSeq: YP_440846.1
UniProt: Q2T1V3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MCGIVGAVAQ RNIVPVLIEG LRRLEYRGYD SCGVAVLGAG APAPGAPRRA RSVARVADLD AQVRESHLGG VTGIAHTRWA THGAPVTHNA HPIFSSNALA LVHNGIIENF EPLREGLRAK GYAFVSQTDT EVIAHLIHSL YRGNLFAAVQ EAVKQLHGAY AIAVIHQDEP HTVVGARQGS PLVVGFGEQE NFLASDALAL AGSTDRFTFL EEGDVCELTL AGVTIVDRDG ARAVREVRVV AAYGGAVELG PYRHFMQKEI FEQPRAIADT IAQADAFDPS LFGERAGSVF AGIDSLLILA CGTSYYSGLT AKYWLESIAK IPTQVEIASE YRYRESVPNP RALVVVISQS GETADTLAAL KHAQALGHAH TLAICNVATS AMVRQTELQF LTHAGTEIGV ASTKAFTTQL VALFVLAATL GKLRGHVDAA READYLKQLR HLPAALNSVL ALEPQIIAWS EEFARKENAL FLGRGLHYPI SLEGALKLKE ISYIHAEAYP AGELKHGPLA LVTEAMPVVT VAPNDTLLEK LKSNMQEVRA RGGELYVFAD ADTHIVNDEG LHVIRMPEHY GPLSPILHVV PLQLLAYHTA CARGTDVDKP RNLAKSVTVE
NT Sequence
atgtgcggga ttgtcggcgc ggttgcgcaa cgtaacatcg ttccggtgct gatcgaagga ctgcgccgtc tcgagtatcg cggctacgac tcgtgcggcg tcgcggtgct cggcgccggc gcgcctgcgc cgggcgcgcc gaggcgcgcg cgcagcgtcg cgcgcgtcgc cgatctcgat gcgcaggtgc gcgagtcgca tctcggaggc gtaaccggca tcgcgcacac gcgctgggcg acgcacggcg cgcccgtcac gcacaacgcg cacccgatct tctcgtcgaa cgcgctcgcg ctcgtgcaca acggcatcat cgagaacttc gagccgctgc gcgaggggct gcgcgcgaag ggctacgcgt tcgtatcgca gaccgacacc gaagtcatcg cgcacctgat ccacagcctc tatcgcggca acctgttcgc ggccgtgcag gaagccgtca agcagttgca cggcgcgtac gcgatcgcgg tgatccacca ggacgagccg cacaccgtcg tcggcgcgcg ccagggctcg ccgctcgtcg tcggcttcgg cgaacaagag aacttcctcg cgtccgacgc gctcgcgctc gcgggcagca ccgatcgctt cacgttcctc gaggaaggcg acgtctgcga gctgacgctc gcgggcgtga cgatcgtcga tcgcgacggc gcgcgcgccg tgcgcgaggt gcgcgtcgtc gccgcatacg gcggcgcggt cgagctcggc ccgtatcggc acttcatgca gaaggaaatc ttcgagcagc cgcgcgcgat cgccgacacg attgcgcaag cggacgcgtt cgatccgtcg ctgttcggcg agcgcgcggg cagcgtattc gcgggcatcg acagcctgct gatcctcgcg tgcggcacca gctactactc gggcctcacc gcgaagtact ggctcgaatc gatcgcgaaa atcccgacgc aagtcgagat cgcgagcgaa taccgctatc gcgaatcggt gccgaacccg cgcgcgctcg tcgtcgtgat ctcgcagtcg ggcgagacgg ccgatacgct cgccgcgctc aagcacgcgc aggcgctcgg ccacgcgcac acgctcgcga tctgcaacgt cgcgacgagc gcgatggtgc gccagaccga gctgcagttc ctcacgcacg cgggcaccga gatcggcgtc gcttcgacga aggcgttcac gacgcagctc gtcgcgctgt tcgtgctcgc ggcgacgctc ggcaagctgc gcggccacgt cgacgcggcc cgcgaagccg attacctgaa gcagttgcgc cacctgcccg ccgcgctcaa cagcgtgctc gcgctcgagc cgcagatcat cgcgtggtcc gaagagttcg cgcgtaagga aaacgcgctg ttcctcggcc gtgggctgca ctatccgatc tcgctcgaag gcgcgctgaa gctcaaggag atctcgtaca tccacgcgga agcgtatccg gcgggcgaac tgaagcacgg gccgctcgcg ctcgtgacgg aggcgatgcc ggtcgtcacc gtcgcgccga acgacacgct gctcgagaag ctgaaatcga acatgcagga agtgcgcgcg cgcggcggcg agctgtacgt gttcgccgac gcggacacgc acatcgtcaa cgacgaaggg ctgcacgtga tccggatgcc cgagcattac gggccgctgt cgccgatcct gcacgtcgtg ccgctgcagt tgctcgcgta tcacaccgcg tgcgcgcgcg gcaccgacgt cgacaagccg cgcaatctcg cgaaatccgt gacggtggag taa
Details for ButhA.17926.a.A1.GE32930
HARVESTED ON: 7/12/2011
SEQUENCED ON: 7/28/2011
EXPECTED MW: 67kDa
OBSERVED MW: 67kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 96
Validated AA Sequence
MCGIVGAVAQ RNIVPVLIEG LRRLEYRGYD SCGVAVLGAG APAPGAPRRA RSVARVADLD AQVRESHLGG VTGIAHTRWA THGAPVTHNA HPIFSSNALA LVHNGIIENF EPLREGLRAK GYAFVSQTDT EVIAHLIHSL YRGNLFAAVQ EAVKQLHGAY AIAVIHQDEP HTVVGARQGS PLVVGFGEQE NFLASDALAL AGSTDRFTFL EEGDVCELTL AGVTIVDRDG ARAVREVRVV AAYGGAVELG PYRHFMQXEI FEQPRAIADT IAQADAFDPS LFGERAGSXX XGIXXXLILA CGTSYYSGXT AKYWLESIAK XXTQVEIASE YRYRESVPNP RALVVVISQS GETADTLAAL KHAQALGHAH TLAICNVATS AMVRQTELQF LTHAGTEIGV ASTKAFTTQL VALFVLAATL GKLRGHVDAA READYLKQLR HLPAALNSVL ALEPQIIAWS EEFARKENAL FLGRGLHYPI SLEGALKLKE ISYIHAEAYP AGELKHGPLA LVTEAMPVVT VAPNDTLLEK LKSNMQEVRA RGGELYVFAD ADTHIVNDEG LHVIRMPEHY GPLSPILHVV PLQLLAYHTA CARGTDVDKP RNLAKSVT
Validated NT Sequence
accgtcacgg atttcgcgag attgcgcggc ttgtcgacgt cggtgccgcg cgcgcacgcg gtgtgatacg cgagcaactg cagcggcacg acgtgcagga tcggcgacag cggcccgtaa tgctcgggca tccggatcac gtgcagccct tcgtcgttga cgatgtgcgt gtccgcgtcg gcgaacacgt acagctcgcc gccgcgcgcg cgcacttcct gcatgttcga tttcagcttc tcgagcagcg tgtcgttcgg cgcgacggtg acgaccggca tcgcctccgt cacgagcgcg agcggcccgt gcttcagttc gcccgccgga tacgcttccg cgtggatgta cgagatctcc ttgagcttca gcgcgccttc gagcgagatc ggatagtgca gcccacggcc gaggaacagc gcgttttcct tacgcgcgaa ctcttcggac cacgcgatga tctgcggctc gagcgcgagc acgctgttga gcgcggcggg caggtggcgc aactgcttca ggtaatcggc ttcgcgggcc gcgtcgacgt ggccgcgcag cttgccgagc gtcgccgcga gcacgaacag cgcgacgagc tgcgtcgtga acgccttcgt cgaagcgacg ccgatctcgg tgcccgcgtg cgtgaggaac tgcagctcgg tctggcgcac catcgcgctc gtcgcgacgt tgcagatcgc gagcgtgtgc gcgtggccga gcgcctgcgc gtgcttgagc gcggcgagcg tatcggccgt ctcgcccgac tgcgagatca cgacgacgag cgcgcgcggg ttcggcaccg attcgcgata gcggtattcg ctcgcgatct cgacttgcgt cnggnttttc gcgatcgatt cnagccagta cttcgcggtg angcccgagt agtagctggt gccgcacgcg aggatcagca nncngnngat nccnnngant angctgcccg cgcgctcncc gaacagcgac ggatcgaacg cgtccgcttg cgcaatcgtg tcggcgatcg cgcgcggctg ctcgaagatt tcnttctgca tgaagtgccg atacgggccg agctcgaccg cgccgccgta tgcggcgacg acgcgcacct cgcgcacggc gcgcgcgccg tcgcgatcga cgatcgtcac gcccgcgagc gtcagctcgc agacgtcgcc ttcctcgagg aacgtgaagc gatcggtgct gcccgcgagc gcgagcgcgt cggacgcgag gaagttctct tgttcgccga agccgacgac gagcggcgag ccctggcgcg cgccgacgac ggtgtgcggc tcgtcctggt ggatcaccgc gatcgcgtac gcgccgtgca actgcttgac ggcttcctgc acggccgcga acaggttgcc gcgatagagg ctgtggatca ggtgcgcgat gacttcggtg tcggtctgcg atacgaacgc gtagcccttc gcgcgcagcc cctcgcgcag cggctcgaag ttctcgatga tgccgttgtg cacgagcgcg agcgcgttcg acgagaagat cgggtgcgcg ttgtgcgtga cgggcgcgcc gtgcgtcgcc cagcgcgtgt gcgcgatgcc ggttacgcct ccgagatgcg actcgcgcac ctgcgcatcg agatcggcga cgcgcgcgac gctgcgcgcg cgcctcggcg cgcccggcgc aggcgcgccg gcgccgagca ccgcgacgcc gcacgagtcg tagccgcgat actcgagacg gcgcagtcct tcgatcagca ccggaacgat gttacgttgc gcaaccgcgc cgacaatccc gcacatcgaa ccaggaccct gggtctgagc ttccagggta cc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMCGIVGAVA QRNIVPVLIE GLRRLEYRGY DSCGVAVLGA GAPAPGAPRR ARSVARVADL DAQVRESHLG GVTGIAHTRW ATHGAPVTHN AHPIFSSNAL ALVHNGIIEN FEPLREGLRA KGYAFVSQTD TEVIAHLIHS LYRGNLFAAV QEAVKQLHGA YAIAVIHQDE PHTVVGARQG SPLVVGFGEQ ENFLASDALA LAGSTDRFTF LEEGDVCELT LAGVTIVDRD GARAVREVRV VAAYGGAVEL GPYRHFMQKE IFEQPRAIAD TIAQADAFDP SLFGERAGSV FAGIDSLLIL ACGTSYYSGL TAKYWLESIA KIPTQVEIAS EYRYRESVPN PRALVVVISQ SGETADTLAA LKHAQALGHA HTLAICNVAT SAMVRQTELQ FLTHAGTEIG VASTKAFTTQ LVALFVLAAT LGKLRGHVDA AREADYLKQL RHLPAALNSV LALEPQIIAW SEEFARKENA LFLGRGLHYP ISLEGALKLK EISYIHAEAY PAGELKHGPL ALVTEAMPVV TVAPNDTLLE KLKSNMQEVR ARGGELYVFA DADTHIVNDE GLHVIRMPEH YGPLSPILHV VPLQLLAYHT ACARGTDVDK PRNLAKSVTV E
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtgcgggatt gtcggcgcgg ttgcgcaacg taacatcgtt ccggtgctga tcgaaggact gcgccgtctc gagtatcgcg gctacgactc gtgcggcgtc gcggtgctcg gcgccggcgc gcctgcgccg ggcgcgccga ggcgcgcgcg cagcgtcgcg cgcgtcgccg atctcgatgc gcaggtgcgc gagtcgcatc tcggaggcgt aaccggcatc gcgcacacgc gctgggcgac gcacggcgcg cccgtcacgc acaacgcgca cccgatcttc tcgtcgaacg cgctcgcgct cgtgcacaac ggcatcatcg agaacttcga gccgctgcgc gaggggctgc gcgcgaaggg ctacgcgttc gtatcgcaga ccgacaccga agtcatcgcg cacctgatcc acagcctcta tcgcggcaac ctgttcgcgg ccgtgcagga agccgtcaag cagttgcacg gcgcgtacgc gatcgcggtg atccaccagg acgagccgca caccgtcgtc ggcgcgcgcc agggctcgcc gctcgtcgtc ggcttcggcg aacaagagaa cttcctcgcg tccgacgcgc tcgcgctcgc gggcagcacc gatcgcttca cgttcctcga ggaaggcgac gtctgcgagc tgacgctcgc gggcgtgacg atcgtcgatc gcgacggcgc gcgcgccgtg cgcgaggtgc gcgtcgtcgc cgcatacggc ggcgcggtcg agctcggccc gtatcggcac ttcatgcaga aggaaatctt cgagcagccg cgcgcgatcg ccgacacgat tgcgcaagcg gacgcgttcg atccgtcgct gttcggcgag cgcgcgggca gcgtattcgc gggcatcgac agcctgctga tcctcgcgtg cggcaccagc tactactcgg gcctcaccgc gaagtactgg ctcgaatcga tcgcgaaaat cccgacgcaa gtcgagatcg cgagcgaata ccgctatcgc gaatcggtgc cgaacccgcg cgcgctcgtc gtcgtgatct cgcagtcggg cgagacggcc gatacgctcg ccgcgctcaa gcacgcgcag gcgctcggcc acgcgcacac gctcgcgatc tgcaacgtcg cgacgagcgc gatggtgcgc cagaccgagc tgcagttcct cacgcacgcg ggcaccgaga tcggcgtcgc ttcgacgaag gcgttcacga cgcagctcgt cgcgctgttc gtgctcgcgg cgacgctcgg caagctgcgc ggccacgtcg acgcggcccg cgaagccgat tacctgaagc agttgcgcca cctgcccgcc gcgctcaaca gcgtgctcgc gctcgagccg cagatcatcg cgtggtccga agagttcgcg cgtaaggaaa acgcgctgtt cctcggccgt gggctgcact atccgatctc gctcgaaggc gcgctgaagc tcaaggagat ctcgtacatc cacgcggaag cgtatccggc gggcgaactg aagcacgggc cgctcgcgct cgtgacggag gcgatgccgg tcgtcaccgt cgcgccgaac gacacgctgc tcgagaagct gaaatcgaac atgcaggaag tgcgcgcgcg cggcggcgag ctgtacgtgt tcgccgacgc ggacacgcac atcgtcaacg acgaagggct gcacgtgatc cggatgcccg agcattacgg gccgctgtcg ccgatcctgc acgtcgtgcc gctgcagttg ctcgcgtatc acaccgcgtg cgcgcgcggc accgacgtcg acaagccgcg caatctcgcg aaatccgtga cggtggagta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for ButhA.17926.a.A1.PS01193
PURIFICATION DATe: 9/1/2011
CONCENTRATION: 41.2mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 100µl
PERCENT IDENTITY: 98
PERCENT COVERAGE: 96
Protocol Notes
notes unavailable
Validated AA Sequence
MCGIVGAVAQ RNIVPVLIEG LRRLEYRGYD SCGVAVLGAG APAPGAPRRA RSVARVADLD AQVRESHLGG VTGIAHTRWA THGAPVTHNA HPIFSSNALA LVHNGIIENF EPLREGLRAK GYAFVSQTDT EVIAHLIHSL YRGNLFAAVQ EAVKQLHGAY AIAVIHQDEP HTVVGARQGS PLVVGFGEQE NFLASDALAL AGSTDRFTFL EEGDVCELTL AGVTIVDRDG ARAVREVRVV AAYGGAVELG PYRHFMQXEI FEQPRAIADT IAQADAFDPS LFGERAGSXX XGIXXXLILA CGTSYYSGXT AKYWLESIAK XXTQVEIASE YRYRESVPNP RALVVVISQS GETADTLAAL KHAQALGHAH TLAICNVATS AMVRQTELQF LTHAGTEIGV ASTKAFTTQL VALFVLAATL GKLRGHVDAA READYLKQLR HLPAALNSVL ALEPQIIAWS EEFARKENAL FLGRGLHYPI SLEGALKLKE ISYIHAEAYP AGELKHGPLA LVTEAMPVVT VAPNDTLLEK LKSNMQEVRA RGGELYVFAD ADTHIVNDEG LHVIRMPEHY GPLSPILHVV PLQLLAYHTA CARGTDVDKP RNLAKSVT
Validated NT Sequence
accgtcacgg atttcgcgag attgcgcggc ttgtcgacgt cggtgccgcg cgcgcacgcg gtgtgatacg cgagcaactg cagcggcacg acgtgcagga tcggcgacag cggcccgtaa tgctcgggca tccggatcac gtgcagccct tcgtcgttga cgatgtgcgt gtccgcgtcg gcgaacacgt acagctcgcc gccgcgcgcg cgcacttcct gcatgttcga tttcagcttc tcgagcagcg tgtcgttcgg cgcgacggtg acgaccggca tcgcctccgt cacgagcgcg agcggcccgt gcttcagttc gcccgccgga tacgcttccg cgtggatgta cgagatctcc ttgagcttca gcgcgccttc gagcgagatc ggatagtgca gcccacggcc gaggaacagc gcgttttcct tacgcgcgaa ctcttcggac cacgcgatga tctgcggctc gagcgcgagc acgctgttga gcgcggcggg caggtggcgc aactgcttca ggtaatcggc ttcgcgggcc gcgtcgacgt ggccgcgcag cttgccgagc gtcgccgcga gcacgaacag cgcgacgagc tgcgtcgtga acgccttcgt cgaagcgacg ccgatctcgg tgcccgcgtg cgtgaggaac tgcagctcgg tctggcgcac catcgcgctc gtcgcgacgt tgcagatcgc gagcgtgtgc gcgtggccga gcgcctgcgc gtgcttgagc gcggcgagcg tatcggccgt ctcgcccgac tgcgagatca cgacgacgag cgcgcgcggg ttcggcaccg attcgcgata gcggtattcg ctcgcgatct cgacttgcgt cnggnttttc gcgatcgatt cnagccagta cttcgcggtg angcccgagt agtagctggt gccgcacgcg aggatcagca nncngnngat nccnnngant angctgcccg cgcgctcncc gaacagcgac ggatcgaacg cgtccgcttg cgcaatcgtg tcggcgatcg cgcgcggctg ctcgaagatt tcnttctgca tgaagtgccg atacgggccg agctcgaccg cgccgccgta tgcggcgacg acgcgcacct cgcgcacggc gcgcgcgccg tcgcgatcga cgatcgtcac gcccgcgagc gtcagctcgc agacgtcgcc ttcctcgagg aacgtgaagc gatcggtgct gcccgcgagc gcgagcgcgt cggacgcgag gaagttctct tgttcgccga agccgacgac gagcggcgag ccctggcgcg cgccgacgac ggtgtgcggc tcgtcctggt ggatcaccgc gatcgcgtac gcgccgtgca actgcttgac ggcttcctgc acggccgcga acaggttgcc gcgatagagg ctgtggatca ggtgcgcgat gacttcggtg tcggtctgcg atacgaacgc gtagcccttc gcgcgcagcc cctcgcgcag cggctcgaag ttctcgatga tgccgttgtg cacgagcgcg agcgcgttcg acgagaagat cgggtgcgcg ttgtgcgtga cgggcgcgcc gtgcgtcgcc cagcgcgtgt gcgcgatgcc ggttacgcct ccgagatgcg actcgcgcac ctgcgcatcg agatcggcga cgcgcgcgac gctgcgcgcg cgcctcggcg cgcccggcgc aggcgcgccg gcgccgagca ccgcgacgcc gcacgagtcg tagccgcgat actcgagacg gcgcagtcct tcgatcagca ccggaacgat gttacgttgc gcaaccgcgc cgacaatccc gcacatcgaa ccaggaccct gggtctgagc ttccagggta cc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMCGIVGAVA QRNIVPVLIE GLRRLEYRGY DSCGVAVLGA GAPAPGAPRR ARSVARVADL DAQVRESHLG GVTGIAHTRW ATHGAPVTHN AHPIFSSNAL ALVHNGIIEN FEPLREGLRA KGYAFVSQTD TEVIAHLIHS LYRGNLFAAV QEAVKQLHGA YAIAVIHQDE PHTVVGARQG SPLVVGFGEQ ENFLASDALA LAGSTDRFTF LEEGDVCELT LAGVTIVDRD GARAVREVRV VAAYGGAVEL GPYRHFMQKE IFEQPRAIAD TIAQADAFDP SLFGERAGSV FAGIDSLLIL ACGTSYYSGL TAKYWLESIA KIPTQVEIAS EYRYRESVPN PRALVVVISQ SGETADTLAA LKHAQALGHA HTLAICNVAT SAMVRQTELQ FLTHAGTEIG VASTKAFTTQ LVALFVLAAT LGKLRGHVDA AREADYLKQL RHLPAALNSV LALEPQIIAW SEEFARKENA LFLGRGLHYP ISLEGALKLK EISYIHAEAY PAGELKHGPL ALVTEAMPVV TVAPNDTLLE KLKSNMQEVR ARGGELYVFA DADTHIVNDE GLHVIRMPEH YGPLSPILHV VPLQLLAYHT ACARGTDVDK PRNLAKSVTV E
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTGCGGGATT GTCGGCGCGG TTGCGCAACG TAACATCGTT CCGGTGCTGA TCGAAGGACT GCGCCGTCTC GAGTATCGCG GCTACGACTC GTGCGGCGTC GCGGTGCTCG GCGCCGGCGC GCCTGCGCCG GGCGCGCCGA GGCGCGCGCG CAGCGTCGCG CGCGTCGCCG ATCTCGATGC GCAGGTGCGC GAGTCGCATC TCGGAGGCGT AACCGGCATC GCGCACACGC GCTGGGCGAC GCACGGCGCG CCCGTCACGC ACAACGCGCA CCCGATCTTC TCGTCGAACG CGCTCGCGCT CGTGCACAAC GGCATCATCG AGAACTTCGA GCCGCTGCGC GAGGGGCTGC GCGCGAAGGG CTACGCGTTC GTATCGCAGA CCGACACCGA AGTCATCGCG CACCTGATCC ACAGCCTCTA TCGCGGCAAC CTGTTCGCGG CCGTGCAGGA AGCCGTCAAG CAGTTGCACG GCGCGTACGC GATCGCGGTG ATCCACCAGG ACGAGCCGCA CACCGTCGTC GGCGCGCGCC AGGGCTCGCC GCTCGTCGTC GGCTTCGGCG AACAAGAGAA CTTCCTCGCG TCCGACGCGC TCGCGCTCGC GGGCAGCACC GATCGCTTCA CGTTCCTCGA GGAAGGCGAC GTCTGCGAGC TGACGCTCGC GGGCGTGACG ATCGTCGATC GCGACGGCGC GCGCGCCGTG CGCGAGGTGC GCGTCGTCGC CGCATACGGC GGCGCGGTCG AGCTCGGCCC GTATCGGCAC TTCATGCAGA AGGAAATCTT CGAGCAGCCG CGCGCGATCG CCGACACGAT TGCGCAAGCG GACGCGTTCG ATCCGTCGCT GTTCGGCGAG CGCGCGGGCA GCGTATTCGC GGGCATCGAC AGCCTGCTGA TCCTCGCGTG CGGCACCAGC TACTACTCGG GCCTCACCGC GAAGTACTGG CTCGAATCGA TCGCGAAAAT CCCGACGCAA GTCGAGATCG CGAGCGAATA CCGCTATCGC GAATCGGTGC CGAACCCGCG CGCGCTCGTC GTCGTGATCT CGCAGTCGGG CGAGACGGCC GATACGCTCG CCGCGCTCAA GCACGCGCAG GCGCTCGGCC ACGCGCACAC GCTCGCGATC TGCAACGTCG CGACGAGCGC GATGGTGCGC CAGACCGAGC TGCAGTTCCT CACGCACGCG GGCACCGAGA TCGGCGTCGC TTCGACGAAG GCGTTCACGA CGCAGCTCGT CGCGCTGTTC GTGCTCGCGG CGACGCTCGG CAAGCTGCGC GGCCACGTCG ACGCGGCCCG CGAAGCCGAT TACCTGAAGC AGTTGCGCCA CCTGCCCGCC GCGCTCAACA GCGTGCTCGC GCTCGAGCCG CAGATCATCG CGTGGTCCGA AGAGTTCGCG CGTAAGGAAA ACGCGCTGTT CCTCGGCCGT GGGCTGCACT ATCCGATCTC GCTCGAAGGC GCGCTGAAGC TCAAGGAGAT CTCGTACATC CACGCGGAAG CGTATCCGGC GGGCGAACTG AAGCACGGGC CGCTCGCGCT CGTGACGGAG GCGATGCCGG TCGTCACCGT CGCGCCGAAC GACACGCTGC TCGAGAAGCT GAAATCGAAC ATGCAGGAAG TGCGCGCGCG CGGCGGCGAG CTGTACGTGT TCGCCGACGC GGACACGCAC ATCGTCAACG ACGAAGGGCT GCACGTGATC CGGATGCCCG AGCATTACGG GCCGCTGTCG CCGATCCTGC ACGTCGTGCC GCTGCAGTTG CTCGCGTATC ACACCGCGTG CGCGCGCGGC ACCGACGTCG ACAAGCCGCG CAATCTCGCG AAATCCGTGA CGGTGGAGTA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A