ButhA.17924.a
Type III pantothenate kinase (EC 2.7.1.33) (PanK-III) (Pantothenic acid kinase) (BTH_I0369) (coaX)
| CENTER ID: | ButhA.17924.a |
| ORGANISM: | Burkholderia thailandensis E264 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.17924.a.A1.GE32929 | full length | 1 | 259 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.17924.a.A1.PD00301 | full length | 1 | 259 | ||
| ButhA.17924.a.A1.PS01175 | full length | 1 | 259 |
Structures
4O5F
DEPOSITED: 12/19/2013
DETERMINATION: XRay
CLONE: ButhA.17924.a.A1.GE32929
PROTEIN: ButhA.17924.a.A1.PS01175
4O8K
DEPOSITED: 12/28/2013
DETERMINATION: XRay
CLONE: ButhA.17924.a.A1.GE32929
PROTEIN: ButhA.17924.a.A1.PD00301
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_136076 |
| PATRIC ID: | fig|271848.6.peg.3040 |
| RefSeq: | YP_440927.1 |
| UniProt: | Q2T1M2 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSGVCLLIDA GNSRIKWALA DTGRHFVTSG AFEHADDTPD WSTLPAPRGA WISNVAGDAA AARIDALIDA HWPALPRTVV RACAAQCGVT NGYAEPARLG SDRWAGLIGA HAAFPGEHLL IATFGTATTL EALRADGRFT GGLIAPGWAL MMRSLGMHTA QLPTVSIDAA TSLLDELAAN DAHAPFAIDT PHALSAGCLQ AQAGLIERAW RDLEKAWKAP VRLVLSGGAA DAIVRALTVP HTRHDTLVLT GLALIAHSA
NT Sequence
atgagcggtg tgtgcttgct gatcgacgcg ggcaacagcc gcatcaagtg ggcgctcgcg gacaccgggc gccatttcgt cacgagcggc gcgttcgagc acgcggacga cacgccggac tggtcgacgc tgcccgcgcc gcgcggcgcg tggatctcga atgtcgccgg cgacgcggcc gccgcgcgca tcgacgcgct gatcgacgcg cactggcccg cgctgccgcg caccgtcgtg cgcgcgtgcg ccgcgcagtg cggcgtgacc aacggctacg cggaacccgc gcgcctcggc agcgaccgct gggcggggct catcggcgcg cacgccgcgt ttccgggcga acatctgctg atcgcgacgt tcggcacggc aacgacactc gaagcgctgc gcgcggacgg ccgcttcacg ggcgggctga tcgcgcccgg ctgggcgctg atgatgcgct cgctcggcat gcacaccgcg cagctgccga cggtgtcgat cgacgcggcg acgagcctgc tcgacgagct cgccgcgaac gacgcgcacg cgccgttcgc gatcgacacg ccgcatgcgc tgtccgccgg ctgcctgcag gcgcaggcgg ggctcatcga gcgcgcgtgg cgcgatctcg agaaagcgtg gaaggcgccc gtgcggctcg tgctgtcggg cggcgcggcc gatgcgatcg tgcgcgcgct gaccgtcccg cacacgcggc acgacacgct cgtgttgacg ggcctcgcgc tgatcgcgca ttcggcgtga
Details for ButhA.17924.a.A1.GE32929
| HARVESTED ON: | 7/12/2011 |
| SEQUENCED ON: | 3/3/2015 |
| EXPECTED MW: | 29kDa |
| OBSERVED MW: | 29kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSGVCLLID AGNSRIKWAL ADTGRHFVTS GAFEHADDTP DWSTLPAPRG AWISNVAGDA AAARIDALID AHWPALPRTV VRACAAQCGV TNGYAEPARL GSDRWAGLIG AHAAFPGEHL LIATFGTATT LEALRADGRF TGGLIAPGWA LMMRSLGMHT AQLPTVSIDA ATSLLDELAA NDAHAPFAID TPHALSAGCL QAQAGLIERA WRDLEKAWKA PVRLVLSGGA ADAIVRALTV PHTRHDTLVL TGLALIAHSA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagcg gtgtgtgctt gctgatcgac gcgggcaaca gccgcatcaa gtgggcgctc gcggacaccg ggcgccattt cgtcacgagc ggcgcgttcg agcacgcgga cgacacgccg gactggtcga cgctgcccgc gccgcgcggc gcgtggatct cgaatgtcgc cggcgacgcg gccgccgcgc gcatcgacgc gctgatcgac gcgcactggc ccgcgctgcc gcgcaccgtc gtgcgcgcgt gcgccgcgca gtgcggcgtg accaacggct acgcggaacc cgcgcgcctc ggcagcgacc gctgggcggg gctcatcggc gcgcacgccg cgtttccggg cgaacatctg ctgatcgcga cgttcggcac ggcaacgaca ctcgaagcgc tgcgcgcgga cggccgcttc acgggcgggc tgatcgcgcc cggctgggcg ctgatgatgc gctcgctcgg catgcacacc gcgcagctgc cgacggtgtc gatcgacgcg gcgacgagcc tgctcgacga gctcgccgcg aacgacgcgc acgcgccgtt cgcgatcgac acgccgcatg cgctgtccgc cggctgcctg caggcgcagg cggggctcat cgagcgcgcg tggcgcgatc tcgagaaagc gtggaaggcg cccgtgcggc tcgtgctgtc gggcggcgcg gccgatgcga tcgtgcgcgc gctgaccgtc ccgcacacgc ggcacgacac gctcgtgttg acgggcctcg cgctgatcgc gcattcggcg taataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSGVCLLID AGNSRIKWAL ADTGRHFVTS GAFEHADDTP DWSTLPAPRG AWISNVAGDA AAARIDALID AHWPALPRTV VRACAAQCGV TNGYAEPARL GSDRWAGLIG AHAAFPGEHL LIATFGTATT LEALRADGRF TGGLIAPGWA LMMRSLGMHT AQLPTVSIDA ATSLLDELAA NDAHAPFAID TPHALSAGCL QAQAGLIERA WRDLEKAWKA PVRLVLSGGA ADAIVRALTV PHTRHDTLVL TGLALIAHSA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcggtgtg tgcttgctga tcgacgcggg caacagccgc atcaagtggg cgctcgcgga caccgggcgc catttcgtca cgagcggcgc gttcgagcac gcggacgaca cgccggactg gtcgacgctg cccgcgccgc gcggcgcgtg gatctcgaat gtcgccggcg acgcggccgc cgcgcgcatc gacgcgctga tcgacgcgca ctggcccgcg ctgccgcgca ccgtcgtgcg cgcgtgcgcc gcgcagtgcg gcgtgaccaa cggctacgcg gaacccgcgc gcctcggcag cgaccgctgg gcggggctca tcggcgcgca cgccgcgttt ccgggcgaac atctgctgat cgcgacgttc ggcacggcaa cgacactcga agcgctgcgc gcggacggcc gcttcacggg cgggctgatc gcgcccggct gggcgctgat gatgcgctcg ctcggcatgc acaccgcgca gctgccgacg gtgtcgatcg acgcggcgac gagcctgctc gacgagctcg ccgcgaacga cgcgcacgcg ccgttcgcga tcgacacgcc gcatgcgctg tccgccggct gcctgcaggc gcaggcgggg ctcatcgagc gcgcgtggcg cgatctcgag aaagcgtgga aggcgcccgt gcggctcgtg ctgtcgggcg gcgcggccga tgcgatcgtg cgcgcgctga ccgtcccgca cacgcggcac gacacgctcg tgttgacggg cctcgcgctg atcgcgcatt cggcgtaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for ButhA.17924.a.A1.PD00301
| PURIFICATION DATe: | 11/26/2013 |
| CONCENTRATION: | 19.98mg/ml |
| OBSERVED MW: | 29kDa |
| EXPRESSION LEVEL: | Data Unavailable |
| PROTEIN PURIFICATION BUFFER: | 20mM Tris-HCl, pH 8.0, 200mM NaCl, 1% glycerol, 1mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 81 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSGVCLLID AGNSRIKWAL ADTGRHFVTS GAFEHADDTP DWSTLPAPRG AWISNVAGDA AAARIDALID AHWPALPRTV VRACAAQCGV TNGYAEPARL GSDRWAGLIG AHAAFPGEHL LIATFGTATT LEALRADGRF TGGLIAPGWA LMMRSLGMHT AQLPTVSIDA ATSLLDELAA NDAHAPFAID TPHALSAGCL QAQAGLIERA WRDLEKAWKA PVRLVLSGGA ADAIVRALTV PHTRHDTLVL TGLALIAHSA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagcg gtgtgtgctt gctgatcgac gcgggcaaca gccgcatcaa gtgggcgctc gcggacaccg ggcgccattt cgtcacgagc ggcgcgttcg agcacgcgga cgacacgccg gactggtcga cgctgcccgc gccgcgcggc gcgtggatct cgaatgtcgc cggcgacgcg gccgccgcgc gcatcgacgc gctgatcgac gcgcactggc ccgcgctgcc gcgcaccgtc gtgcgcgcgt gcgccgcgca gtgcggcgtg accaacggct acgcggaacc cgcgcgcctc ggcagcgacc gctgggcggg gctcatcggc gcgcacgccg cgtttccggg cgaacatctg ctgatcgcga cgttcggcac ggcaacgaca ctcgaagcgc tgcgcgcgga cggccgcttc acgggcgggc tgatcgcgcc cggctgggcg ctgatgatgc gctcgctcgg catgcacacc gcgcagctgc cgacggtgtc gatcgacgcg gcgacgagcc tgctcgacga gctcgccgcg aacgacgcgc acgcgccgtt cgcgatcgac acgccgcatg cgctgtccgc cggctgcctg caggcgcagg cggggctcat cgagcgcgcg tggcgcgatc tcgagaaagc gtggaaggcg cccgtgcggc tcgtgctgtc gggcggcgcg gccgatgcga tcgtgcgcgc gctgaccgtc ccgcacacgc ggcacgacac gctcgtgttg acgggcctcg cgctgatcgc gcattcggcg taataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSGVCLLID AGNSRIKWAL ADTGRHFVTS GAFEHADDTP DWSTLPAPRG AWISNVAGDA AAARIDALID AHWPALPRTV VRACAAQCGV TNGYAEPARL GSDRWAGLIG AHAAFPGEHL LIATFGTATT LEALRADGRF TGGLIAPGWA LMMRSLGMHT AQLPTVSIDA ATSLLDELAA NDAHAPFAID TPHALSAGCL QAQAGLIERA WRDLEKAWKA PVRLVLSGGA ADAIVRALTV PHTRHDTLVL TGLALIAHSA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCGGTGTG TGCTTGCTGA TCGACGCGGG CAACAGCCGC ATCAAGTGGG CGCTCGCGGA CACCGGGCGC CATTTCGTCA CGAGCGGCGC GTTCGAGCAC GCGGACGACA CGCCGGACTG GTCGACGCTG CCCGCGCCGC GCGGCGCGTG GATCTCGAAT GTCGCCGGCG ACGCGGCCGC CGCGCGCATC GACGCGCTGA TCGACGCGCA CTGGCCCGCG CTGCCGCGCA CCGTCGTGCG CGCGTGCGCC GCGCAGTGCG GCGTGACCAA CGGCTACGCG GAACCCGCGC GCCTCGGCAG CGACCGCTGG GCGGGGCTCA TCGGCGCGCA CGCCGCGTTT CCGGGCGAAC ATCTGCTGAT CGCGACGTTC GGCACGGCAA CGACACTCGA AGCGCTGCGC GCGGACGGCC GCTTCACGGG CGGGCTGATC GCGCCCGGCT GGGCGCTGAT GATGCGCTCG CTCGGCATGC ACACCGCGCA GCTGCCGACG GTGTCGATCG ACGCGGCGAC GAGCCTGCTC GACGAGCTCG CCGCGAACGA CGCGCACGCG CCGTTCGCGA TCGACACGCC GCATGCGCTG TCCGCCGGCT GCCTGCAGGC GCAGGCGGGG CTCATCGAGC GCGCGTGGCG CGATCTCGAG AAAGCGTGGA AGGCGCCCGT GCGGCTCGTG CTGTCGGGCG GCGCGGCCGA TGCGATCGTG CGCGCGCTGA CCGTCCCGCA CACGCGGCAC GACACGCTCG TGTTGACGGG CCTCGCGCTG ATCGCGCATT CGGCGTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA
Details for ButhA.17924.a.A1.PS01175
| PURIFICATION DATe: | 8/31/2011 |
| CONCENTRATION: | 36.13mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 1 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSGVCLLID AGNSRIKWAL ADTGRHFVTS GAFEHADDTP DWSTLPAPRG AWISNVAGDA AAARIDALID AHWPALPRTV VRACAAQCGV TNGYAEPARL GSDRWAGLIG AHAAFPGEHL LIATFGTATT LEALRADGRF TGGLIAPGWA LMMRSLGMHT AQLPTVSIDA ATSLLDELAA NDAHAPFAID TPHALSAGCL QAQAGLIERA WRDLEKAWKA PVRLVLSGGA ADAIVRALTV PHTRHDTLVL TGLALIAHSA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagcg gtgtgtgctt gctgatcgac gcgggcaaca gccgcatcaa gtgggcgctc gcggacaccg ggcgccattt cgtcacgagc ggcgcgttcg agcacgcgga cgacacgccg gactggtcga cgctgcccgc gccgcgcggc gcgtggatct cgaatgtcgc cggcgacgcg gccgccgcgc gcatcgacgc gctgatcgac gcgcactggc ccgcgctgcc gcgcaccgtc gtgcgcgcgt gcgccgcgca gtgcggcgtg accaacggct acgcggaacc cgcgcgcctc ggcagcgacc gctgggcggg gctcatcggc gcgcacgccg cgtttccggg cgaacatctg ctgatcgcga cgttcggcac ggcaacgaca ctcgaagcgc tgcgcgcgga cggccgcttc acgggcgggc tgatcgcgcc cggctgggcg ctgatgatgc gctcgctcgg catgcacacc gcgcagctgc cgacggtgtc gatcgacgcg gcgacgagcc tgctcgacga gctcgccgcg aacgacgcgc acgcgccgtt cgcgatcgac acgccgcatg cgctgtccgc cggctgcctg caggcgcagg cggggctcat cgagcgcgcg tggcgcgatc tcgagaaagc gtggaaggcg cccgtgcggc tcgtgctgtc gggcggcgcg gccgatgcga tcgtgcgcgc gctgaccgtc ccgcacacgc ggcacgacac gctcgtgttg acgggcctcg cgctgatcgc gcattcggcg taataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSGVCLLID AGNSRIKWAL ADTGRHFVTS GAFEHADDTP DWSTLPAPRG AWISNVAGDA AAARIDALID AHWPALPRTV VRACAAQCGV TNGYAEPARL GSDRWAGLIG AHAAFPGEHL LIATFGTATT LEALRADGRF TGGLIAPGWA LMMRSLGMHT AQLPTVSIDA ATSLLDELAA NDAHAPFAID TPHALSAGCL QAQAGLIERA WRDLEKAWKA PVRLVLSGGA ADAIVRALTV PHTRHDTLVL TGLALIAHSA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCGGTGTG TGCTTGCTGA TCGACGCGGG CAACAGCCGC ATCAAGTGGG CGCTCGCGGA CACCGGGCGC CATTTCGTCA CGAGCGGCGC GTTCGAGCAC GCGGACGACA CGCCGGACTG GTCGACGCTG CCCGCGCCGC GCGGCGCGTG GATCTCGAAT GTCGCCGGCG ACGCGGCCGC CGCGCGCATC GACGCGCTGA TCGACGCGCA CTGGCCCGCG CTGCCGCGCA CCGTCGTGCG CGCGTGCGCC GCGCAGTGCG GCGTGACCAA CGGCTACGCG GAACCCGCGC GCCTCGGCAG CGACCGCTGG GCGGGGCTCA TCGGCGCGCA CGCCGCGTTT CCGGGCGAAC ATCTGCTGAT CGCGACGTTC GGCACGGCAA CGACACTCGA AGCGCTGCGC GCGGACGGCC GCTTCACGGG CGGGCTGATC GCGCCCGGCT GGGCGCTGAT GATGCGCTCG CTCGGCATGC ACACCGCGCA GCTGCCGACG GTGTCGATCG ACGCGGCGAC GAGCCTGCTC GACGAGCTCG CCGCGAACGA CGCGCACGCG CCGTTCGCGA TCGACACGCC GCATGCGCTG TCCGCCGGCT GCCTGCAGGC GCAGGCGGGG CTCATCGAGC GCGCGTGGCG CGATCTCGAG AAAGCGTGGA AGGCGCCCGT GCGGCTCGTG CTGTCGGGCG GCGCGGCCGA TGCGATCGTG CGCGCGCTGA CCGTCCCGCA CACGCGGCAC GACACGCTCG TGTTGACGGG CCTCGCGCTG ATCGCGCATT CGGCGTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA