SSGCID: Target Page

ButhA.17922.a

50S ribosomal protein L2 (BTH_I3065) (rplB)

CENTER ID:	ButhA.17922.a
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.17922.a.A1.GE32851	full length		1	275

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.5956
RefSeq:	YP_443569.1
UniProt:	Q2SU30

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAIVKVKPTS PGRRAMVKVV NKDLHKGKPH AALLDTQNSK AGRNNNGRIT TRHQGGGHKH HYRVIDFRRT KDGIPAKVER LEYDPNRSAN IALVLYADGE RRYIIAPKGV TVGQQLMSGS EAPIRAGNTL PIRNIPVGTT IHCIEMLPGK GAQMARSAGT SAMLLAREGV YAQVRLRSGE IRRVHIECRA TIGEVGNEEH SLRQIGKAGA NRWRGIRPTV RGVAMNPIDH PHGGGEGRTA AGRDPVSPWG TPTKGFRTRR NKRTTTMIVQ RRHKR

NT Sequence

atggcaatcg tcaaagtcaa gccgacatcg ccgggtcgcc gcgcgatggt caaggtggtc aacaaggatc tgcacaaggg caagccgcac gcagcgctgc tcgacacgca gaactccaag gctggccgca acaacaacgg tcgcattacg acgcgtcacc agggcggcgg tcacaagcac cactaccgcg tgatcgattt ccgtcgcacg aaggacggca tcccggcgaa ggtcgagcgt ctcgagtacg acccgaaccg cagcgcgaac atcgcgctcg tgctgtacgc agacggcgag cgccgctaca tcatcgcgcc gaagggcgtg acggtgggcc agcagctgat gtcgggctcg gaagcgccga tccgcgcggg caacacgctg ccgatccgca acattccggt cggtacgacg attcactgca tcgaaatgct gccgggcaag ggcgcgcaga tggcgcgttc ggctggtacg tcggcgatgc tgctcgcacg cgaaggcgtg tacgcgcagg ttcgtctgcg ttcgggcgaa atccgccgcg tgcatatcga gtgccgcgcg acgatcggtg aagtcggtaa cgaagagcac agcctgcgcc agatcggcaa ggccggtgcg aaccgctggc gcggtatccg cccgacggtg cgtggcgtcg caatgaaccc gatcgatcac ccgcacggtg gtggtgaggg ccgtactgct gcgggccgcg acccggtgag cccgtggggc acgccgacga agggcttccg tacgcgtcgc aacaagcgca cgacgacgat gatcgtccag cgccgtcaca agcgttaa

Details for ButhA.17922.a.A1.GE32851

HARVESTED ON:	6/28/2011
SEQUENCED ON:	7/22/2011
EXPECTED MW:	32kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMAIVKVKPT SPGRRAMVKV VNKDLHKGKP HAALLDTQNS KAGRNNNGRI TTRHQGGGHK HHYRVIDFRR TKDGIPAKVE RLEYDPNRSA NIALVLYADG ERRYIIAPKG VTVGQQLMSG SEAPIRAGNT LPIRNIPVGT TIHCIEMLPG KGAQMARSAG TSAMLLAREG VYAQVRLRSG EIRRVHIECR ATIGEVGNEE HSLRQIGKAG ANRWRGIRPT VRGVAMNPID HPHGGGEGRT AAGRDPVSPW GTPTKGFRTR RNKRTTTMIV QRRHKR

Validated NT Sequence

agaacttgtt cgtgtgttta ttaacgcttg tgacggcgct ggacgatcat cgtcgtcgtg cgcttgttgc gacgcgtacg gaagcccttc gtcggcgtgc cccacgggct caccgggtcg cggcccgcag cagtacggcc ctcaccacca ccgtgcgggt gatcgatcgg gttcattgcg acgccacgca ccgtcgggcg gataccgcgc cagcggttcg caccggcctt gccgatctgg cgcaggctgt gctcttcgtt accgacttca ccgatcgtcg cgcggcactc gatatgcacg cggcggattt cgcccgaacg cagacgaacc tgcgcgtaca cgccttcgcg tgcgagcagc atcgccgacg taccagccga acgcgccatc tgcgcgccct tgcccggcag catttcgatg cagtgaatcg tcgtaccgac cggaatgttg cggatcggca gcgtgttgcc cgcgcggatc ggcgcttccg agcccgacat cagctgctgg cccaccgtca cgcccttcgg cgcgatgatg tagcggcgct cgccgtctgc gtacagcacg agcgcgatgt tcgcgctgcg gttcgggtcg tactcgagac gctcgacctt cgccgggatg ccgtccttcg tgcgacggaa atcgatcacg cggtagtggt gcttgtgacc gccgccctgg tgacgcgtcg taatgcgacc gttgttgttg cggccagcct tggagttctg cgtgtcgagc agcgctgcgt gcggcttgcc cttgtgcaga tccttgttga ccaccttgac catcgcgcgg cgacccggcg atgtcggctt gactttgacg attgccatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca tggtatatct ccntcttaa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcaatcgtc aaagtcaagc cgacatcgcc gggtcgccgc gcgatggtca aggtggtcaa caaggatctg cacaagggca agccgcacgc agcgctgctc gacacgcaga actccaaggc tggccgcaac aacaacggtc gcattacgac gcgtcaccag ggcggcggtc acaagcacca ctaccgcgtg atcgatttcc gtcgcacgaa ggacggcatc ccggcgaagg tcgagcgtct cgagtacgac ccgaaccgca gcgcgaacat cgcgctcgtg ctgtacgcag acggcgagcg ccgctacatc atcgcgccga agggcgtgac ggtgggccag cagctgatgt cgggctcgga agcgccgatc cgcgcgggca acacgctgcc gatccgcaac attccggtcg gtacgacgat tcactgcatc gaaatgctgc cgggcaaggg cgcgcagatg gcgcgttcgg ctggtacgtc ggcgatgctg ctcgcacgcg aaggcgtgta cgcgcaggtt cgtctgcgtt cgggcgaaat ccgccgcgtg catatcgagt gccgcgcgac gatcggtgaa gtcggtaacg aagagcacag cctgcgccag atcggcaagg ccggtgcgaa ccgctggcgc ggtatccgcc cgacggtgcg tggcgtcgca atgaacccga tcgatcaccc gcacggtggt ggtgagggcc gtactgctgc gggccgcgac ccggtgagcc cgtggggcac gccgacgaag ggcttccgta cgcgtcgcaa caagcgcacg acgacgatga tcgtccagcg ccgtcacaag cgttaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa