SSGCID: Target Page

ButhA.17914.a

2-nonaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase (EC 1.14.13.-) (5-demethoxyubiquinone hydroxylase) (DMQ hydroxylase) (BTH_I1108) (coq7)

CENTER ID:	ButhA.17914.a
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.17914.a.A1.GE32843	full length		1	208

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.3864
RefSeq:	YP_441656.1
UniProt:	Q2SZJ3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLFDELISEF DRGLRSIAGV SRMSRPVPQP AAAAPAELSA AERKHAAGLM RVNHVGEVCA QALYQAQKFA TSSSELKEMF EHAAREEEDH LAWTAHRLKD LDSRPSLLNP LWYAGALAIG VVAGRLGDKV SLGFMAETER QVESHLDSHL SELPAADVES RAIVEQMRAD EVKHGKTATD AGGIELPMPA RMLMRAASKV MTSTAYYL

NT Sequence

atgctgtttg acgagttgat ctctgaattt gaccggggcc tgcgctcgat tgcgggcgtg agccgaatga gccgcccggt gccccagccc gccgcggcgg cgcctgccga gctttccgcc gccgagcgca agcatgcggc cgggctgatg cgggtgaatc acgtcggcga agtctgcgcg caggcgctct atcaggcgca gaagttcgcg acgtcttcgt ccgagctcaa ggaaatgttc gagcatgcgg cgcgggagga ggaggaccat ctcgcgtgga ccgcgcatcg tttgaaggat ctcgattcgc ggccgagcct gctgaacccg ctgtggtatg cgggcgcgct cgcgatcggc gtcgttgccg gacgcctcgg cgacaaggtg agcctgggct tcatggccga aacggagcgg caggtcgaga gccatctcga cagccattta tcggagttgc cggctgccga cgtcgagtcg cgcgcgatcg tcgagcagat gcgcgccgac gaagtcaagc acggcaagac ggcgaccgac gcgggcggca tcgagctgcc gatgccggcg cgcatgctga tgcgcgccgc atccaaagtc atgacaagca ctgcatacta tctgtga

Details for ButhA.17914.a.A1.GE32843

HARVESTED ON:	6/28/2011
SEQUENCED ON:	7/22/2011
EXPECTED MW:	24kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMLFDELISE FDRGLRSIAG VSRMSRPVPQ PAAAAPAELS AAERKHAAGL MRVNHVGEVC AQALYQAQKF ATSSSELKEM FEHAAREEED HLAWTAHRLK DLDSRPSLLN PLWYAGALAI GVVAGRLGDK VSLGFMAETE RQVESHLDSH LSELPAADVE SRAIVEQMRA DEVKHGKTAT DAGGIELPMP ARMLMRAASK VMTSTAYYL

Validated NT Sequence

tttnnttnac tttnnnnngg aganatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgctgtt tgacgagttg atctctgaat ttgaccgggg cctgcgctcg attgcgggcg tgagccgaat gagccgcccg gtgccccagc ccgccgcggc ggcgcctgcc gagctttccg ccgccgagcg caagcatgcg gccgggctga tgcgggtgaa tcacgtcggc gaagtctgcg cgcaggcgct ctatcaggcg cagaagttcg cgacgtcttc gtccgagctc aaggaaatgt tcgagcatgc ggcgcgggag gaggaggacc atctcgcgtg gaccgcgcat cgtttgaagg atctcgattc gcggccgagc ctgctgaacc cgctgtggta tgcgggcgcg ctcgcgatcg gcgtcgttgc cggacgcctc ggcgacaagg tgagcctggg cttcatggcc gaaacggagc ggcaggtcga gagccatctc gacagccatt tatcggagtt gccggctgcc gacgtcgagt cgcgcgcgat cgtcgagcag atgcgcgccg acgaagtcaa gcacggcaag acggcgaccg acgcgggcgg catcgagctg ccgatgccgg cgcgcatgct gatgcgcgcc gcatccaaag tcatgacnag cactgcatac tatctgtaat aaacagcacg aacaagttct gcagccnagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttgnct gctgccaccg ctnannnnan a

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gctgtttgac gagttgatct ctgaatttga ccggggcctg cgctcgattg cgggcgtgag ccgaatgagc cgcccggtgc cccagcccgc cgcggcggcg cctgccgagc tttccgccgc cgagcgcaag catgcggccg ggctgatgcg ggtgaatcac gtcggcgaag tctgcgcgca ggcgctctat caggcgcaga agttcgcgac gtcttcgtcc gagctcaagg aaatgttcga gcatgcggcg cgggaggagg aggaccatct cgcgtggacc gcgcatcgtt tgaaggatct cgattcgcgg ccgagcctgc tgaacccgct gtggtatgcg ggcgcgctcg cgatcggcgt cgttgccgga cgcctcggcg acaaggtgag cctgggcttc atggccgaaa cggagcggca ggtcgagagc catctcgaca gccatttatc ggagttgccg gctgccgacg tcgagtcgcg cgcgatcgtc gagcagatgc gcgccgacga agtcaagcac ggcaagacgg cgaccgacgc gggcggcatc gagctgccga tgccggcgcg catgctgatg cgcgccgcat ccaaagtcat gacaagcact gcatactatc tgtaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa