ButhA.17899.a

Folylpolyglutamate synthase/dihydrofolate synthase (BTH_II0683)

CENTER ID: ButhA.17899.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.17899.a.A1.GE32918 full length 1 437
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.17899.a.A1.PS01198 full length 1 437

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.771
RefSeq: YP_438882.1
UniProt: Q2T7G7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSTFSSLDAW LSHLESAHPV GIDMGLTRIG QVKDALKLEF ACPVITVGGT NGKGSTCAFI ETILVRAGYK VGCHTSPHLL RFNERARIDG RIVDDDELLP HFEAVEAART RLAEPVSLTY FEFTTLAIMH LFASRGLDAV ILEVGLGGRL DAVNVIDADC AIVTSIDVDH IEYLGDTREK IAFEKAGIFR PGKPAICGDP APPQTLVDHA AAIGADLWLV GRDFRFSTQP GSERQQWTYA GRDKRYPALA YPALRGANQL LNASAALAAL EALRERLPVS AQDIRLGLAN VELPGRFQVL PGKPLVLLDV AHNPHAAAVL AQNLDSMGYY PYTHAVFGAM ADKDLAGIVA RLKGAIDHWH LTDLPLPRAA SADVLERVLR GAGVENGAQH SVTRHASPAD AFLDALKSAS DNDRIVVFGS FYTVAGVMPV VDRRHDH
NT Sequence
atgagcacct tttcctctct cgacgcgtgg ctttcgcacc tcgaaagcgc gcatcccgtc ggcatcgaca tgggccttac ccgcatcggc caggtcaagg acgcgctgaa gctcgaattc gcgtgcccgg tgatcacggt cggcggcacg aacggcaagg gctcgacctg cgcgttcatc gagacgatcc tcgtgcgcgc gggctacaag gtcggttgcc acacgtcgcc gcatctgctg cgcttcaacg aacgcgcgcg catcgacggc cggatcgtcg acgacgacga actgctgccg catttcgagg cggtcgaggc ggcgcgcacg cgcctggcgg agccggtgtc gctcacgtac ttcgagttca cgacgctcgc gatcatgcat ctgttcgcgt cgcgcgggct cgacgcggtg atcctcgaag tggggctcgg cggccggctt gacgcggtca acgtgatcga cgccgattgc gcgatcgtga cgagcatcga cgtcgaccac atcgaatatc tcggcgacac gcgcgagaag atcgcattcg agaaggcggg catcttccgt ccgggcaagc ccgcgatctg cggcgacccg gcgccgccgc agacgcttgt cgaccatgcg gccgcgatcg gcgcggatct gtggctcgtc gggcgcgact tccgcttctc gacgcagccg ggcagcgagc gccagcaatg gacgtacgcc ggccgcgaca agcgctaccc ggcgctcgcg tatccggcgc tgcggggcgc gaaccagttg ctcaatgcgt cggcggcgct cgccgcgctc gaggcgctgc gcgagcggct gcccgtatcc gcgcaggaca tccggctcgg gctcgcgaac gtcgaactgc cagggcgctt ccaggtgctg cccggcaagc cgctcgtgct gctcgacgtc gcgcacaatc cgcacgcggc cgcggtgctc gcgcagaatc tcgattcgat gggctactac ccgtacacgc atgcggtgtt cggcgcgatg gccgacaagg atctcgcggg aatcgtcgcg cggctgaagg gcgcgatcga tcactggcat ctgaccgatc tgccgctgcc gcgcgcggct tcggccgacg tgctcgagcg cgtgctgcgc ggcgcgggcg tcgagaacgg cgcgcaacac agcgtcacgc gccacgccag tccggccgat gcgttcctcg atgcactaaa aagcgcatcc gacaatgata gaatcgtggt tttcggtagc ttctacacgg tagcgggcgt gatgcccgtc gtggaccgcc gccatgacca ctga
Details for ButhA.17899.a.A1.GE32918
HARVESTED ON: 7/12/2011
SEQUENCED ON: 7/28/2011
EXPECTED MW: 49kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 93
Validated AA Sequence
MSTFSSLDAW LSHLESAHPV GIDMGLTRIG QVKDALKLEF ACPVITVGGT NGKGSTCAFI ETILVRAGYK VGCHTSPHLL RFNERARIDG RIVDDDELLP HFEAVEAART RLAEPVSLTY FEFTTLAIMH LFASRGLDAV ILEVGLGGRL DAVNVIDADC AIVTSIDVDH IEYLGDTREK IAFEKAGIFR PGKPAICGDP APPQTLVDHA AAIGADLWLV GRDFRFSTQP GSERQQWTYA GRDKRYPALA YPALRGANQL LNASAALAAL EALRERLPVS AQDIRLGLAN VELPGRFQVL PGKPLVLLDV AHNPHAAAVL AQNLDSMGYY PYTHAVFGAM ADKDLAGIVA RLKGAIDHWH LTDLPLPRAA SADVLERVLR GAGVENGAQH SVTRHASPAD AFLDALKSAS DNDRIVVFGS FYTVAGV
Validated NT Sequence
catcacgccc gctaccgtgt agaagctacc gaaaaccacg attctatcat tgtcggatgc gctttttagt gcatcgagga acgcatcggc cggactggcg tggcgcgtga cgctgtgttg cgcgccgttc tcgacgcccg cgccgcgcag cacgcgctcg agcacgtcgg ccgaagccgc gcgcggcagc ggcagatcgg tcagatgcca gtgatcgatc gcgcccttca gccgcgcgac gattcccgcg agatccttgt cggccatcgc gccgaacacc gcatgcgtgt acgggtagta gcccatcgaa tcgagattct gcgcgagcac cgcggccgcg tgcggattgt gcgcgacgtc gagcagcacg agcggcttgc cgggcagcac ctggaagcgc cctggcagtt cgacgttcgc gagcccgagc cggatgtcct gcgcggatac gggcagccgc tcgcgcagcg cctcgagcgc ggcgagcgcc gccgacgcat tgagcaactg gttcgcgccc cgcagcgccg gatacgcgag cgccgggtag cgcttgtcgc ggccggcgta cgtccattgc tggcgctcgc tgcccggctg cgtcgagaag cggaagtcgc gcccgacgag ccacagatcc gcgccgatcg cggccgcatg gtcgacaagc gtctgcggcg gcgccgggtc gccgcagatc gcgggcttgc ccggacggaa gatgcccgcc ttctcgaatg cgatcttctc gcgcgtgtcg ccgagatatt cgatgtggtc gacgtcgatg ctcgtcacga tcgcgcaatc ggcgtcgatc acgttgaccg cgtcaagccg gccgccgagc cccacttcga ggatcaccgc gtcgagcccg cgcgacgcga acagatgcat gatcgcgagc gtcgtgaact cgaagtacgt gagcgacacc ggctccgcca ggcgcgtgcg cgccgcctcg accgcctcga aatgcggcag cagttcgtcg tcgtcgacga tccggccgtc gatgcgcgcg cgttcgttga agcgcagcag atgcggcgac gtgtggcaac cgaccttgta gcccgcgcgc acgaggatcg tctcgatgaa cgcgcaggtc gagcccttgc cgttcgtgcc gccgaccgtg atcaccgggc acgcgaattc gagcttcagc gcgtccttga cctggccgat gcgggtaagg cccatgtcga tgccgacggg atgcgcgctt tcgaggtgcg aaagccacgc gtcgagagag gaaaaggtgc tcatcgaacc aggaccctgg gtctgagctt ccagg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSTFSSLDA WLSHLESAHP VGIDMGLTRI GQVKDALKLE FACPVITVGG TNGKGSTCAF IETILVRAGY KVGCHTSPHL LRFNERARID GRIVDDDELL PHFEAVEAAR TRLAEPVSLT YFEFTTLAIM HLFASRGLDA VILEVGLGGR LDAVNVIDAD CAIVTSIDVD HIEYLGDTRE KIAFEKAGIF RPGKPAICGD PAPPQTLVDH AAAIGADLWL VGRDFRFSTQ PGSERQQWTY AGRDKRYPAL AYPALRGANQ LLNASAALAA LEALRERLPV SAQDIRLGLA NVELPGRFQV LPGKPLVLLD VAHNPHAAAV LAQNLDSMGY YPYTHAVFGA MADKDLAGIV ARLKGAIDHW HLTDLPLPRA ASADVLERVL RGAGVENGAQ HSVTRHASPA DAFLDALKSA SDNDRIVVFG SFYTVAGVMP VVDRRHDH
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcaccttt tcctctctcg acgcgtggct ttcgcacctc gaaagcgcgc atcccgtcgg catcgacatg ggccttaccc gcatcggcca ggtcaaggac gcgctgaagc tcgaattcgc gtgcccggtg atcacggtcg gcggcacgaa cggcaagggc tcgacctgcg cgttcatcga gacgatcctc gtgcgcgcgg gctacaaggt cggttgccac acgtcgccgc atctgctgcg cttcaacgaa cgcgcgcgca tcgacggccg gatcgtcgac gacgacgaac tgctgccgca tttcgaggcg gtcgaggcgg cgcgcacgcg cctggcggag ccggtgtcgc tcacgtactt cgagttcacg acgctcgcga tcatgcatct gttcgcgtcg cgcgggctcg acgcggtgat cctcgaagtg gggctcggcg gccggcttga cgcggtcaac gtgatcgacg ccgattgcgc gatcgtgacg agcatcgacg tcgaccacat cgaatatctc ggcgacacgc gcgagaagat cgcattcgag aaggcgggca tcttccgtcc gggcaagccc gcgatctgcg gcgacccggc gccgccgcag acgcttgtcg accatgcggc cgcgatcggc gcggatctgt ggctcgtcgg gcgcgacttc cgcttctcga cgcagccggg cagcgagcgc cagcaatgga cgtacgccgg ccgcgacaag cgctacccgg cgctcgcgta tccggcgctg cggggcgcga accagttgct caatgcgtcg gcggcgctcg ccgcgctcga ggcgctgcgc gagcggctgc ccgtatccgc gcaggacatc cggctcgggc tcgcgaacgt cgaactgcca gggcgcttcc aggtgctgcc cggcaagccg ctcgtgctgc tcgacgtcgc gcacaatccg cacgcggccg cggtgctcgc gcagaatctc gattcgatgg gctactaccc gtacacgcat gcggtgttcg gcgcgatggc cgacaaggat ctcgcgggaa tcgtcgcgcg gctgaagggc gcgatcgatc actggcatct gaccgatctg ccgctgccgc gcgcggcttc ggccgacgtg ctcgagcgcg tgctgcgcgg cgcgggcgtc gagaacggcg cgcaacacag cgtcacgcgc cacgccagtc cggccgatgc gttcctcgat gcactaaaaa gcgcatccga caatgataga atcgtggttt tcggtagctt ctacacggta gcgggcgtga tgcccgtcgt ggaccgccgc catgaccact aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for ButhA.17899.a.A1.PS01198
PURIFICATION DATe: 8/31/2011
CONCENTRATION: 65.25mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 93
Protocol Notes
notes unavailable
Validated AA Sequence
MSTFSSLDAW LSHLESAHPV GIDMGLTRIG QVKDALKLEF ACPVITVGGT NGKGSTCAFI ETILVRAGYK VGCHTSPHLL RFNERARIDG RIVDDDELLP HFEAVEAART RLAEPVSLTY FEFTTLAIMH LFASRGLDAV ILEVGLGGRL DAVNVIDADC AIVTSIDVDH IEYLGDTREK IAFEKAGIFR PGKPAICGDP APPQTLVDHA AAIGADLWLV GRDFRFSTQP GSERQQWTYA GRDKRYPALA YPALRGANQL LNASAALAAL EALRERLPVS AQDIRLGLAN VELPGRFQVL PGKPLVLLDV AHNPHAAAVL AQNLDSMGYY PYTHAVFGAM ADKDLAGIVA RLKGAIDHWH LTDLPLPRAA SADVLERVLR GAGVENGAQH SVTRHASPAD AFLDALKSAS DNDRIVVFGS FYTVAGV
Validated NT Sequence
catcacgccc gctaccgtgt agaagctacc gaaaaccacg attctatcat tgtcggatgc gctttttagt gcatcgagga acgcatcggc cggactggcg tggcgcgtga cgctgtgttg cgcgccgttc tcgacgcccg cgccgcgcag cacgcgctcg agcacgtcgg ccgaagccgc gcgcggcagc ggcagatcgg tcagatgcca gtgatcgatc gcgcccttca gccgcgcgac gattcccgcg agatccttgt cggccatcgc gccgaacacc gcatgcgtgt acgggtagta gcccatcgaa tcgagattct gcgcgagcac cgcggccgcg tgcggattgt gcgcgacgtc gagcagcacg agcggcttgc cgggcagcac ctggaagcgc cctggcagtt cgacgttcgc gagcccgagc cggatgtcct gcgcggatac gggcagccgc tcgcgcagcg cctcgagcgc ggcgagcgcc gccgacgcat tgagcaactg gttcgcgccc cgcagcgccg gatacgcgag cgccgggtag cgcttgtcgc ggccggcgta cgtccattgc tggcgctcgc tgcccggctg cgtcgagaag cggaagtcgc gcccgacgag ccacagatcc gcgccgatcg cggccgcatg gtcgacaagc gtctgcggcg gcgccgggtc gccgcagatc gcgggcttgc ccggacggaa gatgcccgcc ttctcgaatg cgatcttctc gcgcgtgtcg ccgagatatt cgatgtggtc gacgtcgatg ctcgtcacga tcgcgcaatc ggcgtcgatc acgttgaccg cgtcaagccg gccgccgagc cccacttcga ggatcaccgc gtcgagcccg cgcgacgcga acagatgcat gatcgcgagc gtcgtgaact cgaagtacgt gagcgacacc ggctccgcca ggcgcgtgcg cgccgcctcg accgcctcga aatgcggcag cagttcgtcg tcgtcgacga tccggccgtc gatgcgcgcg cgttcgttga agcgcagcag atgcggcgac gtgtggcaac cgaccttgta gcccgcgcgc acgaggatcg tctcgatgaa cgcgcaggtc gagcccttgc cgttcgtgcc gccgaccgtg atcaccgggc acgcgaattc gagcttcagc gcgtccttga cctggccgat gcgggtaagg cccatgtcga tgccgacggg atgcgcgctt tcgaggtgcg aaagccacgc gtcgagagag gaaaaggtgc tcatcgaacc aggaccctgg gtctgagctt ccagg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSTFSSLDA WLSHLESAHP VGIDMGLTRI GQVKDALKLE FACPVITVGG TNGKGSTCAF IETILVRAGY KVGCHTSPHL LRFNERARID GRIVDDDELL PHFEAVEAAR TRLAEPVSLT YFEFTTLAIM HLFASRGLDA VILEVGLGGR LDAVNVIDAD CAIVTSIDVD HIEYLGDTRE KIAFEKAGIF RPGKPAICGD PAPPQTLVDH AAAIGADLWL VGRDFRFSTQ PGSERQQWTY AGRDKRYPAL AYPALRGANQ LLNASAALAA LEALRERLPV SAQDIRLGLA NVELPGRFQV LPGKPLVLLD VAHNPHAAAV LAQNLDSMGY YPYTHAVFGA MADKDLAGIV ARLKGAIDHW HLTDLPLPRA ASADVLERVL RGAGVENGAQ HSVTRHASPA DAFLDALKSA SDNDRIVVFG SFYTVAGVMP VVDRRHDH
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCACCTTT TCCTCTCTCG ACGCGTGGCT TTCGCACCTC GAAAGCGCGC ATCCCGTCGG CATCGACATG GGCCTTACCC GCATCGGCCA GGTCAAGGAC GCGCTGAAGC TCGAATTCGC GTGCCCGGTG ATCACGGTCG GCGGCACGAA CGGCAAGGGC TCGACCTGCG CGTTCATCGA GACGATCCTC GTGCGCGCGG GCTACAAGGT CGGTTGCCAC ACGTCGCCGC ATCTGCTGCG CTTCAACGAA CGCGCGCGCA TCGACGGCCG GATCGTCGAC GACGACGAAC TGCTGCCGCA TTTCGAGGCG GTCGAGGCGG CGCGCACGCG CCTGGCGGAG CCGGTGTCGC TCACGTACTT CGAGTTCACG ACGCTCGCGA TCATGCATCT GTTCGCGTCG CGCGGGCTCG ACGCGGTGAT CCTCGAAGTG GGGCTCGGCG GCCGGCTTGA CGCGGTCAAC GTGATCGACG CCGATTGCGC GATCGTGACG AGCATCGACG TCGACCACAT CGAATATCTC GGCGACACGC GCGAGAAGAT CGCATTCGAG AAGGCGGGCA TCTTCCGTCC GGGCAAGCCC GCGATCTGCG GCGACCCGGC GCCGCCGCAG ACGCTTGTCG ACCATGCGGC CGCGATCGGC GCGGATCTGT GGCTCGTCGG GCGCGACTTC CGCTTCTCGA CGCAGCCGGG CAGCGAGCGC CAGCAATGGA CGTACGCCGG CCGCGACAAG CGCTACCCGG CGCTCGCGTA TCCGGCGCTG CGGGGCGCGA ACCAGTTGCT CAATGCGTCG GCGGCGCTCG CCGCGCTCGA GGCGCTGCGC GAGCGGCTGC CCGTATCCGC GCAGGACATC CGGCTCGGGC TCGCGAACGT CGAACTGCCA GGGCGCTTCC AGGTGCTGCC CGGCAAGCCG CTCGTGCTGC TCGACGTCGC GCACAATCCG CACGCGGCCG CGGTGCTCGC GCAGAATCTC GATTCGATGG GCTACTACCC GTACACGCAT GCGGTGTTCG GCGCGATGGC CGACAAGGAT CTCGCGGGAA TCGTCGCGCG GCTGAAGGGC GCGATCGATC ACTGGCATCT GACCGATCTG CCGCTGCCGC GCGCGGCTTC GGCCGACGTG CTCGAGCGCG TGCTGCGCGG CGCGGGCGTC GAGAACGGCG CGCAACACAG CGTCACGCGC CACGCCAGTC CGGCCGATGC GTTCCTCGAT GCACTAAAAA GCGCATCCGA CAATGATAGA ATCGTGGTTT TCGGTAGCTT CTACACGGTA GCGGGCGTGA TGCCCGTCGT GGACCGCCGC CATGACCACT AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA