SSGCID: Target Page

ButhA.17895.a

Multifunctional CCA protein (BTH_I0236) (cca)

CENTER ID:	ButhA.17895.a
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.17895.a.A1.GE32916	full length		1	432

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.17895.a.A1.PS01192	full length		1	432

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.2889
RefSeq:	YP_440794.3
UniProt:	Q2T205

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGRGRARRDP RDRHLRAVAM KIYAVGGAIR DALLGLPVRD RDYVVVGATP ERMAAQGFKP VGKDFPVFLH PDTREEYALA RTERKTAAGY HGFQFYYAPD VTLEQDLVRR DLTINAMARE VSPDGALVGP VVDPFGGQAD LRAKLFRHVG GAFVEDPVRV LRVARFAARF ADFAVAPETS ALMREMVDAG EVDALVPERV WQELARGLME AKPSRMFAVL RECGALVRIL PEIDALFGVP QRADYHPEVD TGVHVMMVID HAAKQGYSLP VRFAALTHDL GKATTPADAL PRHIGHEGRS VDLLKPLCER LRVPNECRDL ALVVAREHGN LHRVMGMGAA ALVRLFERAD ALRKPARFAE ALQASEADAR GRLGLETTSY PQAERLRQAL VAARSVDAGA IAQGLAGEPA KIKDAVHRAR VRAVAQATGV AE

NT Sequence

atgggtcgag ggcgcgcgcg gcgagacccg cgtgatcgac atctacgggc cgtcgcgatg aagatctacg cggtgggcgg cgcgattcgc gacgcactgc tcggcctgcc ggtgcgtgat cgcgactacg tcgtcgtcgg cgcgacgccc gagcggatgg ccgcgcaagg cttcaagccg gtcggcaagg attttccggt gttcctgcat ccggacacgc gcgaggaata cgcgctcgcg cgcaccgagc gcaagacggc ggccggctat cacggcttcc agttctatta cgcgcccgac gtcacgctcg agcaggatct cgtgcggcgc gacctgacga tcaacgcgat ggcgcgcgaa gtgagcccgg acggcgcgct cgtcgggccg gtcgtcgatc cgttcggcgg gcaggcggat ttgcgcgcga agctgttccg gcacgtcggc ggcgcgttcg tcgaggaccc ggtgcgggtg ctgcgcgtcg cgcgcttcgc cgcgcgcttc gccgattttg cggttgcgcc cgagacgtcc gcgctgatgc gcgagatggt cgacgcgggc gaggtcgacg cgctcgtgcc cgagcgcgtg tggcaggagc tcgcgcgcgg gctgatggaa gcgaagccgt cgcggatgtt tgcggtgctg cgcgagtgcg gcgcgctcgt gcgaatcctg cccgagatcg acgcgctctt cggcgtgccg cagcgcgccg actatcaccc cgaggtcgac acgggcgtgc acgtgatgat ggtgatcgat cacgcggcga agcagggcta ttcgctgccg gtgcgcttcg cggcgctcac gcacgacctc ggcaaggcga cgacgcccgc cgacgcgctg ccgcgccaca tcggccacga gggccgcagc gtcgatctgc tgaagccgct gtgcgagcgg ctgcgggtgc cgaacgagtg ccgcgatctg gcgctcgtcg tcgcgcgcga gcacggcaac ctgcatcggg tgatggggat gggcgcggcc gcgctggtgc gcctgttcga gcgcgccgac gcgctgcgca agccggcgcg cttcgccgag gcgctgcagg cgagcgaggc cgacgcgcgc ggccggctcg gcctcgagac gacgtcctat ccgcaggcgg agcggctgcg gcaggcgctc gtcgccgcgc gttcggtcga tgcgggtgcg atcgcgcagg ggctcgcggg cgagcccgcg aagatcaagg atgcggtgca ccgcgcgcgc gtgcgcgcgg tcgcgcaggc gaccggcgtc gcggagtag

Details for ButhA.17895.a.A1.GE32916

HARVESTED ON:	7/12/2011
SEQUENCED ON:	7/28/2011
EXPECTED MW:	49kDa
OBSERVED MW:	49kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	84
PERCENT COVERAGE:	83

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMGRGRARRD PRDRHLRAVA MKIYAVGGAI RDALLGLPVR DRDYVVVGAT PERMAAQGFK PVGKDFPVFL HPDTREEYAL ARTERKTAAG YHGFQFYYAP DVTLEQDLVR RDLTINAMAR EVSPDGALVG PVVDPFGGQA DLRAKLFRHV GGAFVEDPVR VLRVARFAAR FADFAVAPET SALMREMVDA GEVDALVPER VWQELARGLM EAKPSRMFAV LRECGALVRI LPEIDALFGV PQRADYHPEV DTGVHVMMVI DHAAXQGYSL PXRFAALTHD LXXATXPADA LPXXSGHXXX XVXLLXPLCX XLXXXXXCXX SGXSXSXXXX AXXIGXWGXX XXXXXXXXXX XXXXXXXXX

Validated NT Sequence

ttttgtttna ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgggtc gagggcgcgc gcggcgagac ccgcgtgatc gacatctacg ggccgtcgcg atgaagatct acgcggtggg cggcgcgatt cgcgacgcac tgctcggcct gccggtgcgt gatcgcgact acgtcgtcgt cggcgcgacg cccgagcgga tggccgcgca aggcttcaag ccggtcggca aggattttcc ggtgttcctg catccggaca cgcgcgagga atacgcgctc gcgcgcaccg agcgcaagac ggcggccggc tatcacggct tccagttcta ttacgcgccc gacgtcacgc tcgagcagga tctcgtgcgg cgcgacctga cgatcaacgc gatggcgcgc gaagtgagcc cggacggcgc gctcgtcggg ccggtcgtcg atccgttcgg cgggcaggcg gatttgcgcg cgaagctgtt ccggcacgtc ggcggcgcgt tcgtcgagga cccggtgcgg gtgctgcgcg tcgcgcgctt cgccgcgcgc ttcgccgatt ttgcggttgc gcccgagacg tccgcgctga tgcgcgagat ggtcgacgcg ggcgaggtcg acgcgctcgt gcccgagcgc gtgtggcagg agctcgcgcg cgggctgatg gaagcgaagc cgtcgcggat gtttgcggtg ctgcgcgagt gcggcgcgct cgtgcgaatc ctgcccgaga tcgacgcgct cttcggcgtg ccgcagcgcg ccgactatca ccccgaggtc gacacgggcg tgcacgtgat gatggtgatc gatcacgcng cgaancaggg ctattcgctg ccggngcgct tcgcggcgct cacgcacgac ctcngcnagg cgacnanncc cgccgacgcg ctgccgcnnn catcnggcca cnaggncnnc ancgtcnatc tgctgaancc nctntgcgan nngctgcngn nnncgaanna gtgccnnnna tctggnnnnt cnncgtcnnn nnncnannnn gcaannnnca tcgggnnatg gggnngggnt gntnnnnnnn nnncnannnn nnnnnnannc cnnnnnnntt nntncnnnnn ncnnnnnnng

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMGRGRARRD PRDRHLRAVA MKIYAVGGAI RDALLGLPVR DRDYVVVGAT PERMAAQGFK PVGKDFPVFL HPDTREEYAL ARTERKTAAG YHGFQFYYAP DVTLEQDLVR RDLTINAMAR EVSPDGALVG PVVDPFGGQA DLRAKLFRHV GGAFVEDPVR VLRVARFAAR FADFAVAPET SALMREMVDA GEVDALVPER VWQELARGLM EAKPSRMFAV LRECGALVRI LPEIDALFGV PQRADYHPEV DTGVHVMMVI DHAAKQGYSL PVRFAALTHD LGKATTPADA LPRHIGHEGR SVDLLKPLCE RLRVPNECRD LALVVAREHG NLHRVMGMGA AALVRLFERA DALRKPARFA EALQASEADA RGRLGLETTS YPQAERLRQA LVAARSVDAG AIAQGLAGEP AKIKDAVHRA RVRAVAQATG VAE

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggtcgaggg cgcgcgcggc gagacccgcg tgatcgacat ctacgggccg tcgcgatgaa gatctacgcg gtgggcggcg cgattcgcga cgcactgctc ggcctgccgg tgcgtgatcg cgactacgtc gtcgtcggcg cgacgcccga gcggatggcc gcgcaaggct tcaagccggt cggcaaggat tttccggtgt tcctgcatcc ggacacgcgc gaggaatacg cgctcgcgcg caccgagcgc aagacggcgg ccggctatca cggcttccag ttctattacg cgcccgacgt cacgctcgag caggatctcg tgcggcgcga cctgacgatc aacgcgatgg cgcgcgaagt gagcccggac ggcgcgctcg tcgggccggt cgtcgatccg ttcggcgggc aggcggattt gcgcgcgaag ctgttccggc acgtcggcgg cgcgttcgtc gaggacccgg tgcgggtgct gcgcgtcgcg cgcttcgccg cgcgcttcgc cgattttgcg gttgcgcccg agacgtccgc gctgatgcgc gagatggtcg acgcgggcga ggtcgacgcg ctcgtgcccg agcgcgtgtg gcaggagctc gcgcgcgggc tgatggaagc gaagccgtcg cggatgtttg cggtgctgcg cgagtgcggc gcgctcgtgc gaatcctgcc cgagatcgac gcgctcttcg gcgtgccgca gcgcgccgac tatcaccccg aggtcgacac gggcgtgcac gtgatgatgg tgatcgatca cgcggcgaag cagggctatt cgctgccggt gcgcttcgcg gcgctcacgc acgacctcgg caaggcgacg acgcccgccg acgcgctgcc gcgccacatc ggccacgagg gccgcagcgt cgatctgctg aagccgctgt gcgagcggct gcgggtgccg aacgagtgcc gcgatctggc gctcgtcgtc gcgcgcgagc acggcaacct gcatcgggtg atggggatgg gcgcggccgc gctggtgcgc ctgttcgagc gcgccgacgc gctgcgcaag ccggcgcgct tcgccgaggc gctgcaggcg agcgaggccg acgcgcgcgg ccggctcggc ctcgagacga cgtcctatcc gcaggcggag cggctgcggc aggcgctcgt cgccgcgcgt tcggtcgatg cgggtgcgat cgcgcagggg ctcgcgggcg agcccgcgaa gatcaaggat gcggtgcacc gcgcgcgcgt gcgcgcggtc gcgcaggcga ccggcgtcgc ggagtaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa

Details for ButhA.17895.a.A1.PS01192

PURIFICATION DATe:	8/24/2011
CONCENTRATION:	57.3mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	2
VIAL VOLUME:	200µl
PERCENT IDENTITY:	84
PERCENT COVERAGE:	83

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMGRGRARRD PRDRHLRAVA MKIYAVGGAI RDALLGLPVR DRDYVVVGAT PERMAAQGFK PVGKDFPVFL HPDTREEYAL ARTERKTAAG YHGFQFYYAP DVTLEQDLVR RDLTINAMAR EVSPDGALVG PVVDPFGGQA DLRAKLFRHV GGAFVEDPVR VLRVARFAAR FADFAVAPET SALMREMVDA GEVDALVPER VWQELARGLM EAKPSRMFAV LRECGALVRI LPEIDALFGV PQRADYHPEV DTGVHVMMVI DHAAKQGYSL PVRFAALTHD LGKATTPADA LPRHIGHEGR SVDLLKPLCE RLRVPNECRD LALVVAREHG NLHRVMGMGA AALVRLFERA DALRKPARFA EALQASEADA RGRLGLETTS YPQAERLRQA LVAARSVDAG AIAQGLAGEP AKIKDAVHRA RVRAVAQATG VAE

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGGTCGAGGG CGCGCGCGGC GAGACCCGCG TGATCGACAT CTACGGGCCG TCGCGATGAA GATCTACGCG GTGGGCGGCG CGATTCGCGA CGCACTGCTC GGCCTGCCGG TGCGTGATCG CGACTACGTC GTCGTCGGCG CGACGCCCGA GCGGATGGCC GCGCAAGGCT TCAAGCCGGT CGGCAAGGAT TTTCCGGTGT TCCTGCATCC GGACACGCGC GAGGAATACG CGCTCGCGCG CACCGAGCGC AAGACGGCGG CCGGCTATCA CGGCTTCCAG TTCTATTACG CGCCCGACGT CACGCTCGAG CAGGATCTCG TGCGGCGCGA CCTGACGATC AACGCGATGG CGCGCGAAGT GAGCCCGGAC GGCGCGCTCG TCGGGCCGGT CGTCGATCCG TTCGGCGGGC AGGCGGATTT GCGCGCGAAG CTGTTCCGGC ACGTCGGCGG CGCGTTCGTC GAGGACCCGG TGCGGGTGCT GCGCGTCGCG CGCTTCGCCG CGCGCTTCGC CGATTTTGCG GTTGCGCCCG AGACGTCCGC GCTGATGCGC GAGATGGTCG ACGCGGGCGA GGTCGACGCG CTCGTGCCCG AGCGCGTGTG GCAGGAGCTC GCGCGCGGGC TGATGGAAGC GAAGCCGTCG CGGATGTTTG CGGTGCTGCG CGAGTGCGGC GCGCTCGTGC GAATCCTGCC CGAGATCGAC GCGCTCTTCG GCGTGCCGCA GCGCGCCGAC TATCACCCCG AGGTCGACAC GGGCGTGCAC GTGATGATGG TGATCGATCA CGCGGCGAAG CAGGGCTATT CGCTGCCGGT GCGCTTCGCG GCGCTCACGC ACGACCTCGG CAAGGCGACG ACGCCCGCCG ACGCGCTGCC GCGCCACATC GGCCACGAGG GCCGCAGCGT CGATCTGCTG AAGCCGCTGT GCGAGCGGCT GCGGGTGCCG AACGAGTGCC GCGATCTGGC GCTCGTCGTC GCGCGCGAGC ACGGCAACCT GCATCGGGTG ATGGGGATGG GCGCGGCCGC GCTGGTGCGC CTGTTCGAGC GCGCCGACGC GCTGCGCAAG CCGGCGCGCT TCGCCGAGGC GCTGCAGGCG AGCGAGGCCG ACGCGCGCGG CCGGCTCGGC CTCGAGACGA CGTCCTATCC GCAGGCGGAG CGGCTGCGGC AGGCGCTCGT CGCCGCGCGT TCGGTCGATG CGGGTGCGAT CGCGCAGGGG CTCGCGGGCG AGCCCGCGAA GATCAAGGAT GCGGTGCACC GCGCGCGCGT GCGCGCGGTC GCGCAGGCGA CCGGCGTCGC GGAGTAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA