ButhA.17885.a

Aspartate-semialdehyde dehydrogenase (EC 1.2.1.11) (BTH_II0675) (asd)

CENTER ID:	ButhA.17885.a
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	in PDB
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.17885.a.A1.GE32906	full length		1	373

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Structures

3UW3

DEPOSITED: 11/30/2011

DETERMINATION: XRay

Diffraction Image

CLONE: ButhA.17885.a.A1.GE32906

PROTEIN: ButhA.17885.a.A1.PS01196

Publications by SSGCID

Combining functional and structural genomics to sample the essential Burkholderia structome.

Abendroth J, Armour B, Barrett L, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Fox D, Gallagher LA, Gardberg AS, Gillespie A, Manoil C, Myler PJ, Nakazawa-Hewitt S, Napuli A, Nguyen MT, Patrapuvich R, Phan I, Stacy R, Staker BL, Stewart LJ, Van Voorhis WC

PLoS ONE - 2012

volume 8, issue 1, pages e53851

PMID: 23382856; PMCID: PMC3561365

PDB: 3D63, 3DAH, 3EIZ, 3EJ2, 3EK2, 3EZO, 3F0F, 3FTP, 3GK0, 3GK3, 3GVF, 3GWA, 3GWE, 3IML, 3SZ8, 3T4C, 3TML, 3TMQ, 3TXY, 3U7J, 3UE9, 3UK1, 3UK2, 3UND, 3UPT, 3URR, 3UW1, 3UW2, 3UW3, 3V2I, 3V7N, 3V8H, 3V9O, 3V9P, 3VAV, 4DDO, 4DFE, 4DHE, 4DHK, 4DUT, 4DZ4, 4E4T, 4E51, 4EFI, 4EG0, 4EGJ, 4EK2, 4EQY, 4EWG, 4EX5, 4EXQ, 4F2G, 4F32, 4F3N, 4F3Y, 4F4H, 4F7D, 4FK8, 4FRY, 4G1K, 4G67, 4G6Z, 4GHK, 4H3Y, 4H3Z, 4H4G

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_146655
PATRIC ID:	fig\|271848.6.peg.763
RefSeq:	YP_438874.1
UniProt:	Q2T7H5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNVGLVGWRG MVGSVLMQRM QEEGDFDLIE PVFFSTSNAG GKAPSFAKNE TTLKDATSID DLKKCDVIIT CQGGDYTNDV FPKLRAAGWN GYWIDAASSL RMKDDAVIIL DPVNLNVIKD ALVNGTKNFI GGNCTVSLML MALGGLFREN LVDWMTAMTY QAASGAGAQN MRELLAQMGT LNGAVAAQLA DPASAILDID RRVLAAMNGD AMPTSQFGVP LAGSLIPWID KDLGNGMSRE EWKGGAETNK ILGKPAMGEP GSVPVDGLCV RIGAMRCHSQ ALTIKLKKDV PLDEINGILA SANDWVKVVP NEREASMRDL SPAKVTGTLS VPVGRLRKLA MGGEYLSAFT VGDQLLWGAA EPLRRMLRIL LDK

NT Sequence

atgaacgtag gtctcgtagg ttggcgcggc atggtcggca gcgtgctgat gcagcgcatg caggaagaag gcgatttcga tctgatcgag ccggtgtttt tcagcaccag caacgcgggc ggcaaggcgc cgtcgttcgc gaaaaacgag accacgctca aggatgcgac gagcatcgac gacctgaaga agtgcgacgt gatcatcacg tgccagggcg gcgactacac gaacgacgtg ttcccgaagc tgcgcgcggc cggctggaac ggctactgga tcgacgcggc gtcgtcgctg cggatgaagg acgatgcggt gatcatcctc gatcccgtga acctgaacgt gatcaaggac gcgctcgtca acggcacgaa gaacttcatc ggcggcaact gcaccgtcag cctgatgctg atggcgctgg gcggcctgtt ccgcgagaat ctcgtcgact ggatgacggc gatgacctac caggccgcat cgggcgcggg cgcgcagaac atgcgcgagc tgctcgcgca gatgggtacg ctgaacggcg cggtggcggc gcagctcgcc gatccggcgt cggcgatcct cgacatcgac cgccgcgtgc tcgccgcgat gaacggcgac gcgatgccga cgagccagtt cggcgtgccg ctcgcaggct cgctgattcc gtggatcgac aaggatctcg gcaacgggat gtcgagggaa gaatggaagg gcggcgcgga aaccaacaag atcctcggca agccggcgat gggcgagccg ggctcggttc cggtcgacgg cctgtgcgtg cggatcggcg cgatgcggtg ccactcgcag gcgctcacga tcaagctgaa gaaggacgtg ccgctcgacg agatcaacgg catcctcgcg tcggcgaacg actgggtgaa ggtcgtgccg aacgagcgcg aggcgtcgat gcgcgatctg tcgccagcga aggtgacggg cacgctgtcg gtgccggtcg gccgcctgcg caagctcgcg atgggtggcg aatacctgtc ggcatttacc gtcggcgatc aattgctgtg gggcgcggcc gagccgctgc gccggatgct gcgcattctg ctcgacaagt aa

Details for ButhA.17885.a.A1.GE32906

HARVESTED ON:	7/12/2011
SEQUENCED ON:	7/28/2011
EXPECTED MW:	42kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	92

Validated AA Sequence

Validated NT Sequence

cagcatccgg cgcagcggct cggccgcgcc ccacagcaat tgatcgccga cggtaaatgc cgacaggtat tcgccaccca tcgcgagctt gcgcaggcgg ccgaccggca ccgacagcgt gcccgtcacc ttcgctggcg acagatcgcg catcgacgcc tcgcgctcgt tcggcacgac cttcacccag tcgttcgccg acgcgaggat gccgttgatc tcgtcgagcg gcacgtcctt cttcagcttg atcgtgagcg cctgcgagtg gcaccgcatc gcgccgatcc gcacgcacag gccgtcgacc ggaaccgagc ccggctcgcc catcgccggc ttgccgagga tcttgttggt ttccgcgccg cccttccatt cttccctcga catcccgttg ccgagatcct tgtcgatcca cggaatcagc gagcctgcga gcggcacgcc gaactggctc gtcggcatcg cgtcgccgtt catcgcggcg agcacgcggc ggtcgatgtc gaggatcgcc gacgccggat cggcgagctg cgccgccacc gcgccgttca gcgtacccat ctgcgcgagc agctcgcgca tgttctgcgc gcccgcgccc gatgcggcct ggtaggtcat cgccgtcatc cagtcgacga gattctcgcg gaacaggccg cccagcgcca tcagcatcag gctgacggtg cagttgccgc cgatgaagtt cttcgtgccg ttgacgagcg cgtccttgat cacgttcagg ttcacgggat cgaggatgat caccgcatcg tccttcatcc gcagcgacga cgccgcgtcg atccagtagc cgttccagcc ggccgcgcgc agcttcggga acacgtcgtt cgtgtagtcg ccgccctggc acgtgatgat cacgtcgcac ttcttcaggt cgtcgatgct cgtcgcatcc ttgagcgtgg tctcgttttt cgcgaacgac ggcgccttgc cgcccgcgtt gctggtgctg aaaaacaccg gctcgatcag atcgaaatcg ccttcttcct gcatgcgctg catcagcacg ctgccgacca tgccgcgcca acctacgaga cctacgttca tcgaaccagg accctgggtc tgagcttcca g

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMNVGLVGWR GMVGSVLMQR MQEEGDFDLI EPVFFSTSNA GGKAPSFAKN ETTLKDATSI DDLKKCDVII TCQGGDYTND VFPKLRAAGW NGYWIDAASS LRMKDDAVII LDPVNLNVIK DALVNGTKNF IGGNCTVSLM LMALGGLFRE NLVDWMTAMT YQAASGAGAQ NMRELLAQMG TLNGAVAAQL ADPASAILDI DRRVLAAMNG DAMPTSQFGV PLAGSLIPWI DKDLGNGMSR EEWKGGAETN KILGKPAMGE PGSVPVDGLC VRIGAMRCHS QALTIKLKKD VPLDEINGIL ASANDWVKVV PNEREASMRD LSPAKVTGTL SVPVGRLRKL AMGGEYLSAF TVGDQLLWGA AEPLRRMLRI LLDK

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaacgtaggt ctcgtaggtt ggcgcggcat ggtcggcagc gtgctgatgc agcgcatgca ggaagaaggc gatttcgatc tgatcgagcc ggtgtttttc agcaccagca acgcgggcgg caaggcgccg tcgttcgcga aaaacgagac cacgctcaag gatgcgacga gcatcgacga cctgaagaag tgcgacgtga tcatcacgtg ccagggcggc gactacacga acgacgtgtt cccgaagctg cgcgcggccg gctggaacgg ctactggatc gacgcggcgt cgtcgctgcg gatgaaggac gatgcggtga tcatcctcga tcccgtgaac ctgaacgtga tcaaggacgc gctcgtcaac ggcacgaaga acttcatcgg cggcaactgc accgtcagcc tgatgctgat ggcgctgggc ggcctgttcc gcgagaatct cgtcgactgg atgacggcga tgacctacca ggccgcatcg ggcgcgggcg cgcagaacat gcgcgagctg ctcgcgcaga tgggtacgct gaacggcgcg gtggcggcgc agctcgccga tccggcgtcg gcgatcctcg acatcgaccg ccgcgtgctc gccgcgatga acggcgacgc gatgccgacg agccagttcg gcgtgccgct cgcaggctcg ctgattccgt ggatcgacaa ggatctcggc aacgggatgt cgagggaaga atggaagggc ggcgcggaaa ccaacaagat cctcggcaag ccggcgatgg gcgagccggg ctcggttccg gtcgacggcc tgtgcgtgcg gatcggcgcg atgcggtgcc actcgcaggc gctcacgatc aagctgaaga aggacgtgcc gctcgacgag atcaacggca tcctcgcgtc ggcgaacgac tgggtgaagg tcgtgccgaa cgagcgcgag gcgtcgatgc gcgatctgtc gccagcgaag gtgacgggca cgctgtcggt gccggtcggc cgcctgcgca agctcgcgat gggtggcgaa tacctgtcgg catttaccgt cggcgatcaa ttgctgtggg gcgcggccga gccgctgcgc cggatgctgc gcattctgct cgacaagtaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa