SSGCID: Target Page

ButhA.17879.a

tRNA(Ile)-lysidine synthase (EC 6.3.4.-) (tRNA(Ile)-2-lysyl-cytidine synthase) (tRNA(Ile)-lysidine synthetase) (BTH_I1944) (tilS)

CENTER ID:	ButhA.17879.a
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.17879.a.A1.GE32900	full length		1	494

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.17879.a.A1.PS01205	full length		1	494

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.4751
RefSeq:	YP_442474.1
UniProt:	Q2SX75

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MISPHEFSAD RVVLDAIGVA LSAPPDDAPI AIAYSGGLDS TVLLHAAARI AGAGRCIALH VHHGLSANAD AWLAHCAETA QALGVRFDAA RVDVPRASGQ GVEASARDVR YRALETMCAR YGARALWLAQ HADDQAETVL LQLLRGAGIA GLAAMAPQYR PALADVMRVR PLLHLLRAQL ERYAQQHALR WIDDESNADT RYARNALRVD VLPALAPHFP GFRDALGRAA QHAAAAQRLL DDLAAIDLRA VARDDARVLS RDALVVLDDE RGANLLRYWM RSLGLPGASA ARLAEMMKQL RAARDAYALR VDHAGWRLRL YRDDIQWEAV DGAASAAART MRADAAGDDT GEDTGDDAGD DTLSAARPPE CVLAWRGQEV WRVPGWRGTF VFSPVAAHEP DAVPEALLAA ADLRACARAG GERMRVWRGG PGRTLKNVFQ ERGVPAWQRD VPLLYVGDQL LFVPRIGVNR AAHDGADAPG GWRRIEWRPD MLIA

NT Sequence

gtgatctccc ctcatgaatt ttccgccgat cgcgtcgtgc tcgacgcgat cggcgttgcg ctgtccgcgc cgcccgacga tgcgccgatc gcgatcgcgt acagcggcgg cctcgattcg acggtgctgc tgcacgcggc cgcgcgcatc gcgggcgccg ggcgctgcat cgcgctgcat gtgcaccacg gcttgagcgc gaacgccgac gcgtggctcg cgcattgcgc ggaaactgcg caggcgctcg gcgtgcgatt cgacgcggcg cgcgtcgacg tgccgcgcgc gagcgggcag ggcgtcgagg cgagcgcgcg cgacgtgcgc tatcgcgcgc ttgagacgat gtgcgcgcgg tacggcgcgc gcgcgctgtg gctcgcgcag cacgcggacg atcaggcgga gacggtgctg ctgcaattgc tgcgcggcgc gggtatcgcg gggttggcgg cgatggcgcc gcagtaccgg ccggcgctcg ccgacgtcat gcgcgtgcgt ccgctcctgc atctgttgcg cgcgcagctc gagcgctacg cgcagcagca tgcgctgcgc tggatcgacg acgaatcgaa tgccgatacg cgctatgcgc gcaatgcgct gcgcgtcgac gtgcttcccg cgcttgcgcc gcactttccc ggttttcgcg atgcgctcgg ccgcgccgcg cagcatgcgg cggcggctca gcgtctgctc gacgatctcg ccgcgatcga tctgcgcgcg gtcgcgcgcg acgacgcccg cgtgttgtcg cgcgatgcgc tcgttgtgct cgacgacgag cgcggcgcga atctgctgcg ctactggatg cgctcgctcg ggctgccggg cgcgtcggcc gcgcgccttg ccgagatgat gaagcaactg cgcgcggcgc gcgacgcgta tgcgttgcgc gtcgatcatg cgggatggcg cctgcgtctt tatcgcgacg acattcaatg ggaagcggtc gacggcgctg catcggcggc ggcgcgaacg atgcgcgccg atgcggccgg cgacgacacg ggcgaggaca cgggtgacga cgcgggcgac gacacgctga gcgccgcacg gccgccggaa tgcgtgcttg cctggcgggg gcaggaggtg tggcgcgtgc ccggatggcg cggcaccttc gtattcagcc cggtcgcggc gcacgagccg gatgcggtgc cggaggcgtt gctcgcggcc gccgacctgc gcgcatgcgc gcgcgcggga ggcgagcgca tgcgcgtgtg gcgcggcggc ccggggcgca cgctgaagaa tgtgttccag gagcgcggcg tgccggcctg gcagcgcgac gtcccgctgt tgtacgtggg cgaccaactg ctgttcgtgc cgcgcatcgg cgtgaaccgt gcggcgcacg acggcgccga tgcgcccggc ggctggcggc gcatcgaatg gcggcccgac atgctgattg cgtag

Details for ButhA.17879.a.A1.GE32900

HARVESTED ON:	7/12/2011
SEQUENCED ON:	7/28/2011
EXPECTED MW:	55kDa
OBSERVED MW:	55kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	95

Validated AA Sequence

MVISPHEFSA DRVVLDAIGV ALSAPPDDAP IAIAYSGGLD STVLLHAAAR IAGAGRCIAL HVHHGLSANA DAWLAHCAET AQALGVRFDA ARVDVPRASG QGVEASARDV RYRALETMCA RYGARALWLA QHADDQAETV LLQLLRGAGI AGLAAMAPQY RPALADVMRV RPLLHLLRAQ LERYAQQHAL RWIDDESNAD TRYARNALRV DVLPALAPHF PGFRDALGRA AQHAAAAQRL LDDLAAIDLR AVARDDARVL SRDALVVLDD ERGANLLRYW MRSLGLPGAS AARLAEMMKQ LRAARDAYAL RVDHAGWRLR LYRDDIQWEA VDGAASAAAR TMRADAAGDD TGEDTGDDAG DDTLSAARPP ECVLAWRGQE VWRVPGWRGT FVFSPVAAHE PDAVPEALLA AADLRACARA GGERMRVWRG GPGRTLKNVF QERGVPAWQR DVPLLYVGDQ LLFVPRIGVN RAAHDGADAP GGWRRIEWRP DMLIA

Validated NT Sequence

ttattacgca atcagcatgt cgggccgcca ttcgatgcgc cgccagccgc cgggcgcatc ggcgccgtcg tgcgccgcac ggttcacgcc gatgcgcggc acgaacagca gttggtcgcc cacgtacaac agcgggacgt cgcgctgcca ggccggcacg ccgcgctcct ggaacacatt cttcagcgtg cgccccgggc cgccgcgcca cacgcgcatg cgctcgcctc ccgcgcgcgc gcatgcgcgc aggtcggcgg ccgcgagcaa cgcctccggc accgcatccg gctcgtgcgc cgcgaccggg ctgaatacga aggtgccgcg ccatccgggc acgcgccaca cctcctgccc ccgccaggca agcacgcatt ccggcggccg tgcggcgctc agcgtgtcgt cgcccgcgtc gtcacccgtg tcctcgcccg tgtcgtcgcc ggccgcatcg gcgcgcatcg ttcgcgccgc cgccgatgca gcgccgtcga ccgcttccca ttgaatgtcg tcgcgataaa gacgcaggcg ccatcccgca tgatcgacgc gcaacgcata cgcgtcgcgc gccgcgcgca gttgcttcat catctcggca aggcgcgcgg ccgacgcgcc cggcagcccg agcgagcgca tccagtagcg cagcagattc gcgccgcgct cgtcgtcgag cacaacgagc gcatcgcgcg acaacacgcg ggcgtcgtcg cgcgcgaccg cgcgcagatc gatcgcggcg agatcgtcga gcagacgctg agccgccgcc gcatgctgcg cggcgcggcc gagcgcatcg cgaaaaccgg gaaagtgcgg cgcaagcgcg ggaagcacgt cgacgcgcag cgcattgcgc gcatagcgcg tatcggcatt cgattcgtcg tcgatccagc gcagcgcatg ctgctgcgcg tagcgctcga gctgngcgcg caacagatgc aggagcggac gcacgcgcat gacgtcggcg agcgccggcc ggtactgcgg cgccatcgcc gccaaccccg cgatacccgc gccgcgcagc aattgcagca gcaccgtctc cgcctgatcg tccgcgtgct gcgcgagcca cagcgcgcgc gcgccgtacc gcgcgcacat cgtctcaagc gcgcgatagc gcacgtcgcg cgcgctcgcc tcgacgccct gcccgctcgc gcgcggcacg tcgacgcgcg ccgcgtcgaa tcgcacgccg agcgcctgcg cagtttccgc gcaatgcgcg agccacgcgt cggcgttcgc gctcaagccg tggtgcacat gcagcgcgat gcagcgcccg gcgcccgcga tgcgcgcggc cgcgtgcagc agcaccgtcg aatcgaggcc gccgctgtac gcgatcgcga tcggcgcatc gtcgggcggc gcggacagcg caacgccgat cgcgtcgagc acgacgcgat cggcggaaaa ttcatgaggg gagatcacca tcgaaccagg accctgggtc tgagcttcca gg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVISPHEFS ADRVVLDAIG VALSAPPDDA PIAIAYSGGL DSTVLLHAAA RIAGAGRCIA LHVHHGLSAN ADAWLAHCAE TAQALGVRFD AARVDVPRAS GQGVEASARD VRYRALETMC ARYGARALWL AQHADDQAET VLLQLLRGAG IAGLAAMAPQ YRPALADVMR VRPLLHLLRA QLERYAQQHA LRWIDDESNA DTRYARNALR VDVLPALAPH FPGFRDALGR AAQHAAAAQR LLDDLAAIDL RAVARDDARV LSRDALVVLD DERGANLLRY WMRSLGLPGA SAARLAEMMK QLRAARDAYA LRVDHAGWRL RLYRDDIQWE AVDGAASAAA RTMRADAAGD DTGEDTGDDA GDDTLSAARP PECVLAWRGQ EVWRVPGWRG TFVFSPVAAH EPDAVPEALL AAADLRACAR AGGERMRVWR GGPGRTLKNV FQERGVPAWQ RDVPLLYVGD QLLFVPRIGV NRAAHDGADA PGGWRRIEWR PDMLIA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgatctcc cctcatgaat tttccgccga tcgcgtcgtg ctcgacgcga tcggcgttgc gctgtccgcg ccgcccgacg atgcgccgat cgcgatcgcg tacagcggcg gcctcgattc gacggtgctg ctgcacgcgg ccgcgcgcat cgcgggcgcc gggcgctgca tcgcgctgca tgtgcaccac ggcttgagcg cgaacgccga cgcgtggctc gcgcattgcg cggaaactgc gcaggcgctc ggcgtgcgat tcgacgcggc gcgcgtcgac gtgccgcgcg cgagcgggca gggcgtcgag gcgagcgcgc gcgacgtgcg ctatcgcgcg cttgagacga tgtgcgcgcg gtacggcgcg cgcgcgctgt ggctcgcgca gcacgcggac gatcaggcgg agacggtgct gctgcaattg ctgcgcggcg cgggtatcgc ggggttggcg gcgatggcgc cgcagtaccg gccggcgctc gccgacgtca tgcgcgtgcg tccgctcctg catctgttgc gcgcgcagct cgagcgctac gcgcagcagc atgcgctgcg ctggatcgac gacgaatcga atgccgatac gcgctatgcg cgcaatgcgc tgcgcgtcga cgtgcttccc gcgcttgcgc cgcactttcc cggttttcgc gatgcgctcg gccgcgccgc gcagcatgcg gcggcggctc agcgtctgct cgacgatctc gccgcgatcg atctgcgcgc ggtcgcgcgc gacgacgccc gcgtgttgtc gcgcgatgcg ctcgttgtgc tcgacgacga gcgcggcgcg aatctgctgc gctactggat gcgctcgctc gggctgccgg gcgcgtcggc cgcgcgcctt gccgagatga tgaagcaact gcgcgcggcg cgcgacgcgt atgcgttgcg cgtcgatcat gcgggatggc gcctgcgtct ttatcgcgac gacattcaat gggaagcggt cgacggcgct gcatcggcgg cggcgcgaac gatgcgcgcc gatgcggccg gcgacgacac gggcgaggac acgggtgacg acgcgggcga cgacacgctg agcgccgcac ggccgccgga atgcgtgctt gcctggcggg ggcaggaggt gtggcgcgtg cccggatggc gcggcacctt cgtattcagc ccggtcgcgg cgcacgagcc ggatgcggtg ccggaggcgt tgctcgcggc cgccgacctg cgcgcatgcg cgcgcgcggg aggcgagcgc atgcgcgtgt ggcgcggcgg cccggggcgc acgctgaaga atgtgttcca ggagcgcggc gtgccggcct ggcagcgcga cgtcccgctg ttgtacgtgg gcgaccaact gctgttcgtg ccgcgcatcg gcgtgaaccg tgcggcgcac gacggcgccg atgcgcccgg cggctggcgg cgcatcgaat ggcggcccga catgctgatt gcgtaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa

Details for ButhA.17879.a.A1.PS01205

PURIFICATION DATe:	9/3/2011
CONCENTRATION:	20.03mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	1
VIAL VOLUME:	100µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	95

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGATCTCC CCTCATGAAT TTTCCGCCGA TCGCGTCGTG CTCGACGCGA TCGGCGTTGC GCTGTCCGCG CCGCCCGACG ATGCGCCGAT CGCGATCGCG TACAGCGGCG GCCTCGATTC GACGGTGCTG CTGCACGCGG CCGCGCGCAT CGCGGGCGCC GGGCGCTGCA TCGCGCTGCA TGTGCACCAC GGCTTGAGCG CGAACGCCGA CGCGTGGCTC GCGCATTGCG CGGAAACTGC GCAGGCGCTC GGCGTGCGAT TCGACGCGGC GCGCGTCGAC GTGCCGCGCG CGAGCGGGCA GGGCGTCGAG GCGAGCGCGC GCGACGTGCG CTATCGCGCG CTTGAGACGA TGTGCGCGCG GTACGGCGCG CGCGCGCTGT GGCTCGCGCA GCACGCGGAC GATCAGGCGG AGACGGTGCT GCTGCAATTG CTGCGCGGCG CGGGTATCGC GGGGTTGGCG GCGATGGCGC CGCAGTACCG GCCGGCGCTC GCCGACGTCA TGCGCGTGCG TCCGCTCCTG CATCTGTTGC GCGCGCAGCT CGAGCGCTAC GCGCAGCAGC ATGCGCTGCG CTGGATCGAC GACGAATCGA ATGCCGATAC GCGCTATGCG CGCAATGCGC TGCGCGTCGA CGTGCTTCCC GCGCTTGCGC CGCACTTTCC CGGTTTTCGC GATGCGCTCG GCCGCGCCGC GCAGCATGCG GCGGCGGCTC AGCGTCTGCT CGACGATCTC GCCGCGATCG ATCTGCGCGC GGTCGCGCGC GACGACGCCC GCGTGTTGTC GCGCGATGCG CTCGTTGTGC TCGACGACGA GCGCGGCGCG AATCTGCTGC GCTACTGGAT GCGCTCGCTC GGGCTGCCGG GCGCGTCGGC CGCGCGCCTT GCCGAGATGA TGAAGCAACT GCGCGCGGCG CGCGACGCGT ATGCGTTGCG CGTCGATCAT GCGGGATGGC GCCTGCGTCT TTATCGCGAC GACATTCAAT GGGAAGCGGT CGACGGCGCT GCATCGGCGG CGGCGCGAAC GATGCGCGCC GATGCGGCCG GCGACGACAC GGGCGAGGAC ACGGGTGACG ACGCGGGCGA CGACACGCTG AGCGCCGCAC GGCCGCCGGA ATGCGTGCTT GCCTGGCGGG GGCAGGAGGT GTGGCGCGTG CCCGGATGGC GCGGCACCTT CGTATTCAGC CCGGTCGCGG CGCACGAGCC GGATGCGGTG CCGGAGGCGT TGCTCGCGGC CGCCGACCTG CGCGCATGCG CGCGCGCGGG AGGCGAGCGC ATGCGCGTGT GGCGCGGCGG CCCGGGGCGC ACGCTGAAGA ATGTGTTCCA GGAGCGCGGC GTGCCGGCCT GGCAGCGCGA CGTCCCGCTG TTGTACGTGG GCGACCAACT GCTGTTCGTG CCGCGCATCG GCGTGAACCG TGCGGCGCAC GACGGCGCCG ATGCGCCCGG CGGCTGGCGG CGCATCGAAT GGCGGCCCGA CATGCTGATT GCGTAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA