ButhA.17873.a

Acetyl-CoA carboxylase, biotin carboxyl carrier protein (BTH_I1163) (accB)

CENTER ID: ButhA.17873.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.17873.a.A1.GE32894 full length 1 157
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.3919
RefSeq: YP_441710.1
UniProt: Q2SZD9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDLRKLKTLI DLVSESGISE LEVTEGEGKV RIVKNAPPVY VQPSAGFAPQ VSAPAPVLTV PTEGAGAAAP AAAPAAAAPQ GHVVTSPMVG TFYRAPSPGA DPFVQVGDTV KEGQTLCIIE AMKLLNEIES DKAGVIKEIL VENGQAVEYG QPLFVIG
NT Sequence
atggaccttc gcaagctgaa aactctgatc gacctcgtct ccgaatccgg catctccgag ctggaagtca ccgaaggcga aggcaaggtg cgcatcgtca agaacgcgcc gccggtctat gtgcagccgt cggccggctt cgcgccgcag gtgagcgccc ccgcccctgt actgacggtg ccgaccgaag gcgcaggcgc tgcggcgcct gcagcggcgc ccgcggccgc cgcgccgcag ggccacgtcg tgacgtcgcc gatggtcggc acgttctacc gcgcgccgtc gccgggcgcc gatccgttcg tccaggtcgg cgacacggtc aaggaaggcc agacgctttg catcatcgaa gcgatgaagc tgctgaacga aatcgaatcg gacaaggccg gcgtcatcaa ggaaatcctc gtcgagaacg gccaggccgt cgaatacggc cagccgcttt tcgtgatcgg ctaa
Details for ButhA.17873.a.A1.GE32894
HARVESTED ON: 7/12/2011
SEQUENCED ON: 6/19/2015
EXPECTED MW: 18kDa
OBSERVED MW: 20kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMDLRKLKTL IDLVSESGIS ELEVTEGEGK VRIVKNAPPV YVQPSAGFAP QVSAPAPVLT VPTEGAGAAA PAAAPAAAAP QGHVVTSPMV GTFYRAPSPG ADPFVQVGDT VKEGQTLCII EAMKLLNEIE SDKAGVIKEI LVENGQAVEY GQPLFVIG
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggacc ttcgcaagct gaaaactctg atcgacctcg tctccgaatc cggcatctcc gagctggaag tcaccgaagg cgaaggcaag gtgcgcatcg tcaagaacgc gccgccggtc tatgtgcagc cgtcggccgg cttcgcgccg caggtgagcg cccccgcccc tgtactgacg gtgccgaccg aaggcgcagg cgctgcggcg cctgcagcgg cgcccgcggc cgccgcgccg cagggccacg tcgtgacgtc gccgatggtc ggcacgttct accgcgcgcc gtcgccgggc gccgatccgt tcgtccaggt cggcgacacg gtcaaggaag gccagacgct ttgcatcatc gaagcgatga agctgctgaa cgaaatcgaa tcggacaagg ccggcgtcat caaggaaatc ctcgtcgaga acggccaggc cgtcgaatac ggccagccgc ttttcgtgat cggctaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDLRKLKTL IDLVSESGIS ELEVTEGEGK VRIVKNAPPV YVQPSAGFAP QVSAPAPVLT VPTEGAGAAA PAAAPAAAAP QGHVVTSPMV GTFYRAPSPG ADPFVQVGDT VKEGQTLCII EAMKLLNEIE SDKAGVIKEI LVENGQAVEY GQPLFVIG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggaccttcgc aagctgaaaa ctctgatcga cctcgtctcc gaatccggca tctccgagct ggaagtcacc gaaggcgaag gcaaggtgcg catcgtcaag aacgcgccgc cggtctatgt gcagccgtcg gccggcttcg cgccgcaggt gagcgccccc gcccctgtac tgacggtgcc gaccgaaggc gcaggcgctg cggcgcctgc agcggcgccc gcggccgccg cgccgcaggg ccacgtcgtg acgtcgccga tggtcggcac gttctaccgc gcgccgtcgc cgggcgccga tccgttcgtc caggtcggcg acacggtcaa ggaaggccag acgctttgca tcatcgaagc gatgaagctg ctgaacgaaa tcgaatcgga caaggccggc gtcatcaagg aaatcctcgt cgagaacggc caggccgtcg aatacggcca gccgcttttc gtgatcggct aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa