SSGCID: Target Page

ButhA.17868.a

Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8) (Polynucleotide phosphorylase) (PNPase) (BTH_I1056) (pnp)

CENTER ID:	ButhA.17868.a
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.17868.a.A1.GE32887	full length		1	713

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.3817
RefSeq:	YP_441610.1
UniProt:	Q2SZN9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSLFNKIVKE FQWGQHKVRL ETGEIARQAS GAVLVDVEDT VVLATVVGAK SAKPGQDFFP LTVDYIEKTY SAGKIPGGFF RREGRPSEHE TLTSRLIDRP LRPLFPEGFY NEVQVVIHVL SVNPEVPADI PALIGASAAL AVSGLPFNGP VGAARVAYVN NEYVLNPTRE QIKASRLDLV VAGTERAVLM VESEADQLPE DVMLGAVVFG HEQMQTAIDA IHELVREGGK PEWDWQPAPK DEALNARVTE LAQPELLAAY QIRDKQARST KLKEVYAATS AKLEEEALAA GTVAADKATV GNILFDLEAK IVRGQILSGE PRIDGRDTRT VRPIEIRTGV LPRTHGSALF TRGETQALVV ATLGTKGDEQ IIDALEGEYR DRFMLHYNMP PFATGETGRV GSPKRREIGH GRLAKRALVA CLPSADEFGY SIRVVSEITE SNGSSSMASV CGGCLALMDA GVPMKAHVAG IAMGLILEGN KFAVLTDILG DEDHLGDMDF KVAGTAEGVT ALQMDIKIQG ITKEIMQVAL AQAKEGRMHI LGKMKEAVAG ANTQLSEFAP RMITIKINPE KIRDVIGKGG SVIRALTEET GTTIDISDDG VVTIASTNSE GMAEAKKRIE NITAEIEVGQ VYEGTVLKLL DFGAIVNLLP GKDGLLHISE IVNERVKDIN DYLKEGQQVK VKVIQTDEKG RVRLSAKALL NEAAGQTDTP PQQ

NT Sequence

atgtctctgt tcaataaaat cgtgaaggaa ttccagtggg gccagcacaa ggtgcgcctc gaaaccggcg aaatcgcgcg ccaggctagc ggcgccgtcc tcgtcgacgt cgaggacacc gtcgtgctcg cgaccgtcgt cggcgcgaag tcggcgaagc cggggcagga tttcttcccg ctcactgtcg actacatcga gaagacctac tcggccggca agatcccggg cggtttcttc cgccgcgaag gccgtccgtc cgagcacgaa acgctgacgt cgcgcctcat cgaccggccg ctgcgcccgc tcttcccgga aggtttctat aacgaagtgc aggtcgtgat tcacgtgctg tcggtgaacc cggaagtccc ggcggacatc ccggcgctca tcggcgcatc ggctgcgctc gccgtgtccg gcctgccgtt caacggcccg gtcggcgccg cgcgcgtcgc gtacgtgaac aatgaatacg tgctgaaccc gacgcgcgag cagatcaagg cgtcgcgcct cgacctcgtc gtcgcgggca cggagcgcgc ggtgctgatg gtcgagtccg aggccgatca gttgccggaa gacgtgatgc tcggcgcggt cgtgttcggc catgagcaga tgcagaccgc gatcgacgcg atccacgaac tcgtgcgcga aggcggcaag cccgagtggg actggcagcc ggcgccgaag gacgaggcgc tgaacgcacg cgtgaccgag ctcgcgcagc cggaactgct ggcggcctac cagatccgcg acaagcaggc gcgctcgacg aagctgaagg aagtgtacgc ggcaacctcg gcgaagctgg aggaagaggc gctggcggcc ggcacggtcg cggccgacaa ggcaaccgtc ggcaacatcc tgttcgacct cgaggcaaag atcgtccgcg gccagatcct gagcggcgag ccgcgcatcg acggccgcga cacccgcacc gtgcgcccga tcgagatccg cacgggcgtg ctgccgcgca cccacggctc ggcgctgttc acgcgcggcg agacacaagc gctggtggtc gcgacgctcg gcacgaaggg cgatgagcag atcatcgacg cgctcgaagg cgagtaccgc gaccgcttca tgctccacta caacatgccc ccgttcgcga ccggcgaaac gggccgcgtc ggctcgccga agcgtcgcga aatcggccac ggccggctcg cgaagcgcgc gctcgtcgcg tgcctgccga gcgccgacga attcggctac tcgatccgcg tcgtgtcgga aatcaccgag tcgaacggtt cgtcgtcgat ggcgtcggtg tgcggcggct gcctcgcgct gatggacgcc ggcgtgccga tgaaggcgca cgtcgcgggc atcgcgatgg gcctcatcct cgagggcaac aagttcgcgg tgttgaccga catcctcggc gacgaagatc acctcggcga catggacttc aaggtggccg gcacggcgga aggcgtgacg gcgctgcaga tggacatcaa gatccagggc atcacgaagg aaatcatgca ggtcgcgctc gcgcaggcga aggaaggccg catgcacatc ctcggcaaga tgaaggaagc ggtcgcgggt gcgaacacgc agctgtccga attcgcgccg cgcatgatca cgatcaagat caacccggaa aagatccgtg acgtgatcgg caagggcggt tcggtgatcc gcgcgctgac ggaagaaacc ggcacgacga tcgacatctc cgacgacggc gtcgtgacga ttgcgagcac gaacagcgaa ggcatggccg aagcgaagaa gcgcatcgag aacatcacgg ccgagatcga agtcggtcag gtgtacgaag gcacggtgtt gaagctcctc gatttcggcg cgatcgtgaa cctgctgccg ggcaaggacg gtctgctgca catctccgag atcgtcaacg agcgcgtgaa ggacatcaac gactacctga aggaaggtca gcaggtgaag gtcaaggtga tccagacgga cgagaagggg cgcgtgcgtt tgtcggcgaa ggcgctgctg aacgaggcgg ctgggcagac ggatacgccg ccgcagcagt aa

Details for ButhA.17868.a.A1.GE32887

HARVESTED ON:	7/12/2011
SEQUENCED ON:	7/28/2011
EXPECTED MW:	79kDa
OBSERVED MW:	79kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	82
PERCENT COVERAGE:	51

Validated AA Sequence

Validated NT Sequence

ttgtttaact ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgtctctg ttcaataaaa tcgtgaagga attccagtgg ggccagcaca aggtgcgcct cgaaaccggc gaaatcgcgc gccaggctag cggcgccgtc ctcgtcgacg tcgaggacac cgtcgtgctc gcgaccgtcg tcggcgcgaa gtcggcgaag ccggggcagg atttcttccc gctcactgtc gactacatcg agaagaccta ctcggccggc aagatcccgg gcggtttctt ccgccgcgaa ggccgtccgt ccgagcacga aacgctgacg tcgcgcctca tcgaccggcc gctgcgcccg ctcttcccgg aaggtttcta taacgaagtg caggtcgtga ttcacgtgct gtcggtgaac ccggaagtcc cggcggacat cccggcgctc atcggcgcat cggctgcgct cgccgtgtcc ggcctgccgt tcaacggccc ggtcggcgcc gcgcgcgtcg cgtacgtgaa caatgaatac gtgctgaacc cgacgcgcga gcagatcaag gcgtcgcgcc tcgacctcgt cgtcgcgggc acggagcgcg cggtgctgat ggtcgagtcc gaggccgatc agttgccgga agacgtgatg ctcggcgcgg tcgtgttcgg ccatgagcag atgcagaccg cgatcgacgc gatccacgaa ctcgtgcgcg aangcggcaa gcccgagtgg gactggcagc cggcgccgaa nnacgaggcg ctgaacgcac gcgtgaccga gctcgcgcag ccggaactgc tggcggccta ccagatccgc gacaagcagg cgcgctcgac gaanctgaan gaagtgtacn cggcaacctc ngcgaanctg gangaagang cgctggcggc cggcacggnc gcngccgana ngcaaccgtc ggcannntnc tgntcgacnt cnagcnanna tcgtcncngn ngntcnngan cgncgancnn gnatcgnngn nnnncnncng cnncnncgnc nnatcnanat nngnnnggnn nnnnncnnnn ancnnnnnnn nngnannntn nnannnnnnn nnnnnnnnnn nncngnnnnn aanna

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSLFNKIVK EFQWGQHKVR LETGEIARQA SGAVLVDVED TVVLATVVGA KSAKPGQDFF PLTVDYIEKT YSAGKIPGGF FRREGRPSEH ETLTSRLIDR PLRPLFPEGF YNEVQVVIHV LSVNPEVPAD IPALIGASAA LAVSGLPFNG PVGAARVAYV NNEYVLNPTR EQIKASRLDL VVAGTERAVL MVESEADQLP EDVMLGAVVF GHEQMQTAID AIHELVREGG KPEWDWQPAP KDEALNARVT ELAQPELLAA YQIRDKQARS TKLKEVYAAT SAKLEEEALA AGTVAADKAT VGNILFDLEA KIVRGQILSG EPRIDGRDTR TVRPIEIRTG VLPRTHGSAL FTRGETQALV VATLGTKGDE QIIDALEGEY RDRFMLHYNM PPFATGETGR VGSPKRREIG HGRLAKRALV ACLPSADEFG YSIRVVSEIT ESNGSSSMAS VCGGCLALMD AGVPMKAHVA GIAMGLILEG NKFAVLTDIL GDEDHLGDMD FKVAGTAEGV TALQMDIKIQ GITKEIMQVA LAQAKEGRMH ILGKMKEAVA GANTQLSEFA PRMITIKINP EKIRDVIGKG GSVIRALTEE TGTTIDISDD GVVTIASTNS EGMAEAKKRI ENITAEIEVG QVYEGTVLKL LDFGAIVNLL PGKDGLLHIS EIVNERVKDI NDYLKEGQQV KVKVIQTDEK GRVRLSAKAL LNEAAGQTDT PPQQ

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtctctgttc aataaaatcg tgaaggaatt ccagtggggc cagcacaagg tgcgcctcga aaccggcgaa atcgcgcgcc aggctagcgg cgccgtcctc gtcgacgtcg aggacaccgt cgtgctcgcg accgtcgtcg gcgcgaagtc ggcgaagccg gggcaggatt tcttcccgct cactgtcgac tacatcgaga agacctactc ggccggcaag atcccgggcg gtttcttccg ccgcgaaggc cgtccgtccg agcacgaaac gctgacgtcg cgcctcatcg accggccgct gcgcccgctc ttcccggaag gtttctataa cgaagtgcag gtcgtgattc acgtgctgtc ggtgaacccg gaagtcccgg cggacatccc ggcgctcatc ggcgcatcgg ctgcgctcgc cgtgtccggc ctgccgttca acggcccggt cggcgccgcg cgcgtcgcgt acgtgaacaa tgaatacgtg ctgaacccga cgcgcgagca gatcaaggcg tcgcgcctcg acctcgtcgt cgcgggcacg gagcgcgcgg tgctgatggt cgagtccgag gccgatcagt tgccggaaga cgtgatgctc ggcgcggtcg tgttcggcca tgagcagatg cagaccgcga tcgacgcgat ccacgaactc gtgcgcgaag gcggcaagcc cgagtgggac tggcagccgg cgccgaagga cgaggcgctg aacgcacgcg tgaccgagct cgcgcagccg gaactgctgg cggcctacca gatccgcgac aagcaggcgc gctcgacgaa gctgaaggaa gtgtacgcgg caacctcggc gaagctggag gaagaggcgc tggcggccgg cacggtcgcg gccgacaagg caaccgtcgg caacatcctg ttcgacctcg aggcaaagat cgtccgcggc cagatcctga gcggcgagcc gcgcatcgac ggccgcgaca cccgcaccgt gcgcccgatc gagatccgca cgggcgtgct gccgcgcacc cacggctcgg cgctgttcac gcgcggcgag acacaagcgc tggtggtcgc gacgctcggc acgaagggcg atgagcagat catcgacgcg ctcgaaggcg agtaccgcga ccgcttcatg ctccactaca acatgccccc gttcgcgacc ggcgaaacgg gccgcgtcgg ctcgccgaag cgtcgcgaaa tcggccacgg ccggctcgcg aagcgcgcgc tcgtcgcgtg cctgccgagc gccgacgaat tcggctactc gatccgcgtc gtgtcggaaa tcaccgagtc gaacggttcg tcgtcgatgg cgtcggtgtg cggcggctgc ctcgcgctga tggacgccgg cgtgccgatg aaggcgcacg tcgcgggcat cgcgatgggc ctcatcctcg agggcaacaa gttcgcggtg ttgaccgaca tcctcggcga cgaagatcac ctcggcgaca tggacttcaa ggtggccggc acggcggaag gcgtgacggc gctgcagatg gacatcaaga tccagggcat cacgaaggaa atcatgcagg tcgcgctcgc gcaggcgaag gaaggccgca tgcacatcct cggcaagatg aaggaagcgg tcgcgggtgc gaacacgcag ctgtccgaat tcgcgccgcg catgatcacg atcaagatca acccggaaaa gatccgtgac gtgatcggca agggcggttc ggtgatccgc gcgctgacgg aagaaaccgg cacgacgatc gacatctccg acgacggcgt cgtgacgatt gcgagcacga acagcgaagg catggccgaa gcgaagaagc gcatcgagaa catcacggcc gagatcgaag tcggtcaggt gtacgaaggc acggtgttga agctcctcga tttcggcgcg atcgtgaacc tgctgccggg caaggacggt ctgctgcaca tctccgagat cgtcaacgag cgcgtgaagg acatcaacga ctacctgaag gaaggtcagc aggtgaaggt caaggtgatc cagacggacg agaaggggcg cgtgcgtttg tcggcgaagg cgctgctgaa cgaggcggct gggcagacgg atacgccgcc gcagcagtaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa