SSGCID: Target Page

ButhA.17867.b

Electron transfer flavoprotein, alpha subunit (BTH_I1654)

CENTER ID:	ButhA.17867.b
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.17867.b.A1.GE32886	full length		1	311

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.4440
RefSeq:	YP_442191.1
UniProt:	Q2SY08

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTILVIAEHD NASIKAATLN TVAAATKIGG DIHVLVAGHN AQGAADAAAK IAGVSKVLLA DAPQLADGLA ENVEATALNV AKDYSHILAP ATAYGKNIAP RIAAKLDVAQ ISDITAVDSA DTFERPIYAG NAIATVQSSD PIKVITVRAT GFDPVAAQGG GASVEKIDAA ADAGISQFVS REVTKLDRPE LTSASIIVSG GRGLGSGENY TKVLEPLADK LQAALGASRA AVDAGYVPND YQVGQTGKIV APQLYIAVGI SGAIQHLAGM KDSKVIVAIN KDPEAPIFSV ADYGLVGDLF TLVPELVNEL G

NT Sequence

atgacgattc tggttattgc ggaacatgac aacgcgtcga tcaaggccgc cacgctgaac acggtggccg cggcaacgaa gatcggcggc gacattcacg tgctggtggc gggccacaat gcgcagggcg cggcggacgc ggcggcgaag attgcgggcg tgtcgaaggt gctgctggcc gacgcgccgc aactggcgga cggtcttgcg gaaaacgtcg aggcgaccgc gctgaacgtc gcgaaggact actcgcacat cctcgcgccg gcgaccgcgt acggcaagaa catcgcgccg cgcatcgcgg cgaagctgga cgtcgcgcag atctcggaca tcacggcggt cgattcggcc gacacgttcg agcgcccgat ctacgcgggc aacgcgatcg cgacggtgca gtcgagcgat ccgatcaagg tgatcacggt gcgcgcgacg ggcttcgatc cggtcgcggc gcaaggcggc ggcgcgtcgg tcgagaagat cgacgcggcg gcggacgcgg gcatctcgca gttcgtgagc cgcgaagtga cgaagctcga tcgtccggaa ctgacgagcg cgagcatcat cgtgtcgggc ggccggggcc tcggcagcgg cgagaactac acgaaggtgc tggagccgct ggcggacaag ctgcaggcgg cgctgggcgc atcgcgcgcg gcggtggacg cgggctacgt gccgaacgat tatcaagtgg gccagacggg caagatcgtc gcgccgcagc tgtacatcgc ggtgggcatc tcgggcgcga ttcagcacct ggcggggatg aaggattcga aggtgatcgt cgcgatcaac aaggacccgg aagcgccgat cttcagcgtg gccgattacg gcctcgtcgg cgatctgttc acgctcgtgc cggaacttgt gaacgagctc ggctga

Details for ButhA.17867.b.A1.GE32886

HARVESTED ON:	7/12/2011
SEQUENCED ON:	7/28/2011
EXPECTED MW:	33kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	89
PERCENT COVERAGE:	107

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMTILVIAEH DNASIKAATL NTVAAATKIG GDIHVLVAGH NAQGAADAAA KIAGVSKVLL ADAPQLADGL AENVEATALN VAKDYSHILA PATAYGKNIA PRIAAKLDVA QISDITAVDS ADTFERPIYA GNAIATVQSS DPIKVITVRA TGFDPVAAQG GGASVEKIDA AADAGISQFV SREVTKLDRP ELTSASIIVS GGRGLGSGEN YTKVLEPLAD KLXAALGASR AAVDAGYVPN DYQVGQTGKI VAPQLYIAVG ISGAIQHLAG MXDSXGDRRD QQGPXSADLQ RGXXRXXXRS XHAXAXXXXS XXXXSTXXXX XXXXXXAXXX XXXLXXA

Validated NT Sequence

tttnttnnnt ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgacgatt ctggttattg cggaacatga caacgcgtcg atcaaggccg ccacgctgaa cacggtggcc gcggcaacga agatcggcgg cgacattcac gtgctggtgg cgggccacaa tgcgcagggc gcggcggacg cggcggcgaa gattgcgggc gtgtcgaagg tgctgctggc cgacgcgccg caactggcgg acggtcttgc ggaaaacgtc gaggcgaccg cgctgaacgt cgcgaaggac tactcgcaca tcctcgcgcc ggcgaccgcg tacggcaaga acatcgcgcc gcgcatcgcg gcgaagctgg acgtcgcgca gatctcggac atcacggcgg tcgattcggc cgacacgttc gagcgcccga tctacgcggg caacgcgatc gcgacggtgc agtcgagcga tccgatcaag gtgatcacgg tgcgcgcgac gggcttcgat ccggtcgcgg cgcaaggcgg cggcgcgtcg gtcgagaaga tcgacgcggc ggcggacgcg ggcatctcgc agttcgtgag ccgcgaagtg acgaagctcg atcgtccgga actgacgagc gcgagcatca tcgtgtcggg cggccggggc ctcggcagcg gcgagaacta cacgaaggtg ctggagccgc tggcggacaa gctgcangcg gcgctgggcg catcgcgcgc ggcggtggac gcgggctacg tgccgaacga ttatcaagtg ggccagacgg gcaagatcgt cgcgccgcag ctgtacatcg cggtgggcat ctcgggcgcg attcagcacc tggcggggat gaangattcg aanggtgatc gtcgcgatca acaaggaccc ngaagcgccg atcttcagcg tggnnganta cgncntcnnn ggcgatctgn tcacgctcnn gccngnantn nnangagcnc nnctannann agcacnanan nnngcannnn ctnncnnngn ncnncngcnn ncnaanncgn anngnngctg nnnnntgctg c

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMTILVIAEH DNASIKAATL NTVAAATKIG GDIHVLVAGH NAQGAADAAA KIAGVSKVLL ADAPQLADGL AENVEATALN VAKDYSHILA PATAYGKNIA PRIAAKLDVA QISDITAVDS ADTFERPIYA GNAIATVQSS DPIKVITVRA TGFDPVAAQG GGASVEKIDA AADAGISQFV SREVTKLDRP ELTSASIIVS GGRGLGSGEN YTKVLEPLAD KLQAALGASR AAVDAGYVPN DYQVGQTGKI VAPQLYIAVG ISGAIQHLAG MKDSKVIVAI NKDPEAPIFS VADYGLVGDL FTLVPELVNE LG

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacgattctg gttattgcgg aacatgacaa cgcgtcgatc aaggccgcca cgctgaacac ggtggccgcg gcaacgaaga tcggcggcga cattcacgtg ctggtggcgg gccacaatgc gcagggcgcg gcggacgcgg cggcgaagat tgcgggcgtg tcgaaggtgc tgctggccga cgcgccgcaa ctggcggacg gtcttgcgga aaacgtcgag gcgaccgcgc tgaacgtcgc gaaggactac tcgcacatcc tcgcgccggc gaccgcgtac ggcaagaaca tcgcgccgcg catcgcggcg aagctggacg tcgcgcagat ctcggacatc acggcggtcg attcggccga cacgttcgag cgcccgatct acgcgggcaa cgcgatcgcg acggtgcagt cgagcgatcc gatcaaggtg atcacggtgc gcgcgacggg cttcgatccg gtcgcggcgc aaggcggcgg cgcgtcggtc gagaagatcg acgcggcggc ggacgcgggc atctcgcagt tcgtgagccg cgaagtgacg aagctcgatc gtccggaact gacgagcgcg agcatcatcg tgtcgggcgg ccggggcctc ggcagcggcg agaactacac gaaggtgctg gagccgctgg cggacaagct gcaggcggcg ctgggcgcat cgcgcgcggc ggtggacgcg ggctacgtgc cgaacgatta tcaagtgggc cagacgggca agatcgtcgc gccgcagctg tacatcgcgg tgggcatctc gggcgcgatt cagcacctgg cggggatgaa ggattcgaag gtgatcgtcg cgatcaacaa ggacccggaa gcgccgatct tcagcgtggc cgattacggc ctcgtcggcg atctgttcac gctcgtgccg gaacttgtga acgagctcgg ctaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa