ButhA.17866.a

DNA polymerase III, delta prime subunit (BTH_I2155)

CENTER ID: ButhA.17866.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.17866.a.A1.GE32883 full length 1 344
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.4969
RefSeq: YP_442676.1
UniProt: Q2SWM3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIYPWQTDDW ARLQALRAHW PHALLLYGQA GIGKLAFARH LAKGFLCETP RENGEPCGAC AACTWFEQGN HPDYRIVVPE ALAAELPGAA DEPKAADADE GGKKTKTPSK EIKIEQVRAL VDFCGIASHR GGARVVVLYP AEALNVAAAN ALLKTLEEPP SGVVFLLVSA RIDRLLPTIV SRCRQWPMTV PAPDAAAAWL AAQNVADANA LLAEAGGAPL AALALASDEN RPLRDWTLAQ LAAGAACDPF ACGETLQKLP VPVVLGWLQR WLYDLLAERA AGAPRYFPAQ RAALARCAAA VDANAFARLL KTVTRQRAVE NHPLSARLVF EELFLGYREL FASS
NT Sequence
atgatctatc cgtggcaaac cgacgactgg gcccgcctgc aggcgctgcg cgcgcattgg ccgcacgcgc tgttgctgta cggccaggcg ggcatcggca agctcgcctt cgctcggcat ctcgcgaagg gcttcttgtg cgaaacgccg cgcgagaacg gcgagccgtg cggcgcgtgc gccgcgtgca cgtggttcga gcagggcaac caccccgact accggatcgt cgtgccggag gcgctcgcgg ccgagctgcc gggcgcggcc gacgagccga aggccgccga tgccgacgag ggcggcaaga aaaccaagac gccgagcaag gaaatcaaga tcgagcaggt gcgcgcgctt gtcgacttct gcgggatcgc gtcgcatcgc ggcggcgcgc gcgtcgtcgt gctgtatccg gccgaggcgc tcaatgtcgc ggctgcgaac gcgctgctca agacgctcga ggagccgccg tcgggcgtcg tgttcctgct cgtgtccgcg cgcatcgacc ggctgctgcc gacgatcgtg agccgctgcc ggcaatggcc gatgacggtg cccgcacccg atgcggccgc cgcgtggctt gccgcgcaga acgtggcgga cgcgaacgcg cttctcgccg aagcgggcgg cgcgccgctc gccgcgctcg cgctggcgag cgacgagaac cggccgctgc gcgactggac gctcgcacag ctcgcggcgg gcgccgcgtg cgatccgttc gcgtgcggcg agacgctgca gaagctgccc gtgcccgtcg tgctcggctg gctgcagcgc tggctgtacg acctgctcgc cgagcgcgcg gcgggcgcgc cgcgctattt ccccgcgcag cgcgcggcgc tcgcgcgctg cgcggcggcc gtcgacgcga atgcattcgc gcgcttgctg aagacggtca cgcggcagcg cgcggtggag aatcatccgc tcagcgcgcg cctcgtgttc gaagagttgt tcctcggtta tcgcgaattg ttcgcgtcgt cctga
Details for ButhA.17866.a.A1.GE32883
HARVESTED ON: 7/12/2011
SEQUENCED ON: 7/28/2011
EXPECTED MW: 39kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 86
PERCENT COVERAGE: 102
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMIYPWQTDD WARLQALRAH WPHALLLYGQ AGIGKLAFAR HLAKGFLCET PRENGEPCGA CAACTWFEQG NHPDYRIVVP EALAAELPGA ADEPKAADAD EGGKKTKTPS KEIKIEQVRA LVDFCGIASH RGGARVVVLY PAEALNVAAA NALLKTLEEP PSGVVFLLVS ARIDRLLPTI VSRCRQWPMT VPAPDAAAAW LAAQNVADAN ALLAEAGGAP LAALALASDE NRXLRDWTLA QLAAGAACDP FACGETLQKL PVPVXLGWLQ RWXYDLLAER AAGAPRYFPA QRAALARCXA XRXXXIRALA EXXXXXXXXX XSXXSXXXXX XXXXXXXXXX XXXXXXXXXX AXX
Validated NT Sequence
ttttgttnnn tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgatcta tccgtggcaa accgacgact gggcccgcct gcaggcgctg cgcgcgcatt ggccgcacgc gctgttgctg tacggccagg cgggcatcgg caagctcgcc ttcgctcggc atctcgcgaa gggcttcttg tgcgaaacgc cgcgcgagaa cggcgagccg tgcggcgcgt gcgccgcgtg cacgtggttc gagcagggca accaccccga ctaccggatc gtcgtgccgg aggcgctcgc ggccgagctg ccgggcgcgg ccgacgagcc gaaggccgcc gatgccgacg agggcggcaa gaaaaccaag acgccgagca aggaaatcaa gatcgagcag gtgcgcgcgc ttgtcgactt ctgcgggatc gcgtcgcatc gcggcggcgc gcgcgtcgtc gtgctgtatc cggccgaggc gctcaatgtc gcggctgcga acgcgctgct caagacgctc gaggagccgc cgtcgggcgt cgtgttcctg ctcgtgtccg cgcgcatcga ccggctgctg ccgacgatcg tgagccgctg ccggcaatgg ccgatgacgg tgcccgcacc cgatgcggcc gccgcgtggc ttgccgcgca gaacgtggcg gacgcgaacg cgcttctcgc cgaagcgggc ggcgcgccgc tcgccgcgct cgcgctggcg agcgacgaga accggncgct gcgcgactgg acgctcgcac agctcgcggc gggcgccgcg tgcgatccgt tcgcgtgcgg cgagacgctg cagaagctgc ccgtgcccgt cgngctcggc tggctgcagc gctggntgta cgacctgctc gccgagcgcg cggcgggcgc gccgcgctat ttccccgcgc agcgcgcggc gctcgcgcgc tgcncggcnn nncgacncga nngcattcgc gcgcttgctg aannnngncn cnngcnnnnn nnnnnnngnn natcnncnnn ntcannnnnc nnnnnngntc annnnnnnnn nnnntnnnnn nntnnnnnnn cgncctannn aannnnnnna nnnnntnntg cannnnnnnt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMIYPWQTDD WARLQALRAH WPHALLLYGQ AGIGKLAFAR HLAKGFLCET PRENGEPCGA CAACTWFEQG NHPDYRIVVP EALAAELPGA ADEPKAADAD EGGKKTKTPS KEIKIEQVRA LVDFCGIASH RGGARVVVLY PAEALNVAAA NALLKTLEEP PSGVVFLLVS ARIDRLLPTI VSRCRQWPMT VPAPDAAAAW LAAQNVADAN ALLAEAGGAP LAALALASDE NRPLRDWTLA QLAAGAACDP FACGETLQKL PVPVVLGWLQ RWLYDLLAER AAGAPRYFPA QRAALARCAA AVDANAFARL LKTVTRQRAV ENHPLSARLV FEELFLGYRE LFASS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gatctatccg tggcaaaccg acgactgggc ccgcctgcag gcgctgcgcg cgcattggcc gcacgcgctg ttgctgtacg gccaggcggg catcggcaag ctcgccttcg ctcggcatct cgcgaagggc ttcttgtgcg aaacgccgcg cgagaacggc gagccgtgcg gcgcgtgcgc cgcgtgcacg tggttcgagc agggcaacca ccccgactac cggatcgtcg tgccggaggc gctcgcggcc gagctgccgg gcgcggccga cgagccgaag gccgccgatg ccgacgaggg cggcaagaaa accaagacgc cgagcaagga aatcaagatc gagcaggtgc gcgcgcttgt cgacttctgc gggatcgcgt cgcatcgcgg cggcgcgcgc gtcgtcgtgc tgtatccggc cgaggcgctc aatgtcgcgg ctgcgaacgc gctgctcaag acgctcgagg agccgccgtc gggcgtcgtg ttcctgctcg tgtccgcgcg catcgaccgg ctgctgccga cgatcgtgag ccgctgccgg caatggccga tgacggtgcc cgcacccgat gcggccgccg cgtggcttgc cgcgcagaac gtggcggacg cgaacgcgct tctcgccgaa gcgggcggcg cgccgctcgc cgcgctcgcg ctggcgagcg acgagaaccg gccgctgcgc gactggacgc tcgcacagct cgcggcgggc gccgcgtgcg atccgttcgc gtgcggcgag acgctgcaga agctgcccgt gcccgtcgtg ctcggctggc tgcagcgctg gctgtacgac ctgctcgccg agcgcgcggc gggcgcgccg cgctatttcc ccgcgcagcg cgcggcgctc gcgcgctgcg cggcggccgt cgacgcgaat gcattcgcgc gcttgctgaa gacggtcacg cggcagcgcg cggtggagaa tcatccgctc agcgcgcgcc tcgtgttcga agagttgttc ctcggttatc gcgaattgtt cgcgtcgtcc taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa