SSGCID: Target Page

ButhA.17747.b

ParB family protein (BTH_II2372)

CENTER ID:	ButhA.17747.b
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.17747.b.A1.GE32836	full length		1	355

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.17747.b.A1.PW33440	full length		1	355

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.2642
RefSeq:	YP_440559.1
UniProt:	Q2T2P0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKPSQFAKGF QARPDITTSE KRTALDRLNA IEGLVKSEAS TPAVPASKSS KKNIPVTIVE TPPTTDPIDE SSQYRTWRIE NRYQPGQVIE LPLKAIKPSP FNPRHFYLKS SIAELAVNLA KQGQQQAIHV IPDYDNPGSY FVSDGGRRVR ALKEANKESV KAIVIDLPIG IQSYKLGYDL NVQRDSQTVF DNAIVWKRFL EEKYFQSQKE LAEHLGLDES TVAVALSIGK LPEAVMQEMV ARPDRFGSNM AYQVGRYHTA RGTDSTLRLI NKILSDDLST RQVADIVKGR ASAQESPKPA SRQRYAQRHE IKLNGMSVGD LKSYGEDRLE LRLRGLTREK RDEILQQLER MLLSN

NT Sequence

atgaaaccct cccaattcgc caaaggcttc caagcgcgcc ccgacatcac gaccagcgaa aaacgcacgg cgctcgatcg actcaatgcg atcgaaggcc tcgtcaaatc cgaagcctcg acacccgcgg ttcccgccag caaatcgtcg aagaagaaca tcccggtcac aatcgtcgaa acgccgccca ccacggaccc gatcgacgaa tcgagccaat atcgcacctg gcgaatcgag aatcgctatc agcccggcca ggtgatcgaa ctcccgctca aggcgatcaa accgagcccc ttcaacccgc ggcacttcta tttgaagtcg tcgatcgcgg aactggcagt caatctggcg aagcagggtc agcaacaagc gatccacgtc atccccgatt acgacaaccc cggctcgtac ttcgtcagcg acggcggccg ccgcgtccgt gcactgaagg aagcgaacaa ggaatcggtc aaggcgatcg tcatcgacct gccgatcggc attcaaagct acaagctcgg ctacgacctc aacgtccaac gtgattcaca aacggtcttc gataacgcaa tcgtctggaa gcgctttctc gaggaaaagt atttccagag ccagaaggaa ttggccgagc atctcgggct cgacgaatcg acggtcgccg ttgcgctctc aatcggcaag ttgcccgaag ccgtgatgca ggaaatggtt gcgcgcccgg atcgcttcgg ctcgaacatg gcgtatcagg tcggccgtta ccacaccgcg cgcggcaccg actcgacact gcgtctcatc aacaagatcc tgtccgacga tctcagcact cgtcaggtcg ccgatatcgt gaagggaagg gcgagcgcgc aagaaagccc gaagcccgcg agtcgacagc ggtatgcgca gcgacatgag atcaagctga acggcatgtc ggtcggcgat ctgaagtcgt acggcgaaga tcgtctggag ttgcggctgc gcggcctgac gcgagaaaag cgggatgaaa tcctgcagca gctcgaacgg atgctgttgt cgaattga

Details for ButhA.17747.b.A1.GE32836

HARVESTED ON:	6/28/2011
SEQUENCED ON:	7/22/2011
EXPECTED MW:	42kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	94

Validated AA Sequence

Validated NT Sequence

aattcgacaa cagcatccgt tcgagctgct gcaggatttc atcccgcttt tctcgcgtca ggccgcgcag ccgcaactcc agacgatctt cgccgtacga cttcagatcg ccgaccgaca tgccgttcag cttgatctca tgtcgctgcg cataccgctg tcgactcgcg ggcttcgggc tttcttgcgc gctcgccctt cccttcacga tatcggcgac ctgacgagtg ctgagatcgt cggacaggat cttgttgatg agacgcagtg tcgagtcggt gccgcgcgcg gtgtggtaac ggccgacctg atacgccatg ttcgagccga agcgatccgg gcgcgcaacc atttcctgca tcacggcttc gggcaacttg ccgattgaga gcgcaacggc gaccgtcgat tcgtcgagcc cgagatgctc ggccaattcc ttctggctct ggaaatactt ttcctcgaga aagcgcttcc agacgattgc gttatcgaag accgtttgtg aatcacgttg gacgttgagg tcgtagccga gcttgtagct ttgaatgccg atcggcaggt cgatgacgat cgccttgacc gattccttgt tcgcttcctt cagtgcacgg acgcggcggc cgccgtcgct gacgaagtac gagccggggt tgtcgtaatc ggggatgacg tggatcgctt gttgctgacc ctgcttcgcc agattgactg ccagttccgc gatcgacgac ttcaaataga agtgccgcgg gttgaagggg ctcggtttga tcgccttgag cgggagttcg atcacctggc cgggctgata gcgattctcg attcgccagg tgcgatattg gctcgattcg tcgatcgggt ccgtggtggg cggcgtttcg acgattgtga ccgggatgtt cttcttcgac gatttgctgg cgggaaccgc gggtgtcgag gcttcggatt tgacgaggcc ttcgatcgca ttgagtcgat cgagcgccgt gcgtttttcg ctggtcgtga tgtcggggcg cgcttggaag cctttggcga attgggaggg tttcatcgaa ccaggaccct gggtctgagc ttccagggt

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMKPSQFAKG FQARPDITTS EKRTALDRLN AIEGLVKSEA STPAVPASKS SKKNIPVTIV ETPPTTDPID ESSQYRTWRI ENRYQPGQVI ELPLKAIKPS PFNPRHFYLK SSIAELAVNL AKQGQQQAIH VIPDYDNPGS YFVSDGGRRV RALKEANKES VKAIVIDLPI GIQSYKLGYD LNVQRDSQTV FDNAIVWKRF LEEKYFQSQK ELAEHLGLDE STVAVALSIG KLPEAVMQEM VARPDRFGSN MAYQVGRYHT ARGTDSTLRL INKILSDDLS TRQVADIVKG RASAQESPKP ASRQRYAQRH EIKLNGMSVG DLKSYGEDRL ELRLRGLTRE KRDEILQQLE RMLLSN

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaaccctcc caattcgcca aaggcttcca agcgcgcccc gacatcacga ccagcgaaaa acgcacggcg ctcgatcgac tcaatgcgat cgaaggcctc gtcaaatccg aagcctcgac acccgcggtt cccgccagca aatcgtcgaa gaagaacatc ccggtcacaa tcgtcgaaac gccgcccacc acggacccga tcgacgaatc gagccaatat cgcacctggc gaatcgagaa tcgctatcag cccggccagg tgatcgaact cccgctcaag gcgatcaaac cgagcccctt caacccgcgg cacttctatt tgaagtcgtc gatcgcggaa ctggcagtca atctggcgaa gcagggtcag caacaagcga tccacgtcat ccccgattac gacaaccccg gctcgtactt cgtcagcgac ggcggccgcc gcgtccgtgc actgaaggaa gcgaacaagg aatcggtcaa ggcgatcgtc atcgacctgc cgatcggcat tcaaagctac aagctcggct acgacctcaa cgtccaacgt gattcacaaa cggtcttcga taacgcaatc gtctggaagc gctttctcga ggaaaagtat ttccagagcc agaaggaatt ggccgagcat ctcgggctcg acgaatcgac ggtcgccgtt gcgctctcaa tcggcaagtt gcccgaagcc gtgatgcagg aaatggttgc gcgcccggat cgcttcggct cgaacatggc gtatcaggtc ggccgttacc acaccgcgcg cggcaccgac tcgacactgc gtctcatcaa caagatcctg tccgacgatc tcagcactcg tcaggtcgcc gatatcgtga agggaagggc gagcgcgcaa gaaagcccga agcccgcgag tcgacagcgg tatgcgcagc gacatgagat caagctgaac ggcatgtcgg tcggcgatct gaagtcgtac ggcgaagatc gtctggagtt gcggctgcgc ggcctgacgc gagaaaagcg ggatgaaatc ctgcagcagc tcgaacggat gctgttgtcg aattaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa

Details for ButhA.17747.b.A1.PW33440

PURIFICATION DATe:	8/26/2011
CONCENTRATION:	28.15mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	6
VIAL VOLUME:	100µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	94

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAAACCCTCC CAATTCGCCA AAGGCTTCCA AGCGCGCCCC GACATCACGA CCAGCGAAAA ACGCACGGCG CTCGATCGAC TCAATGCGAT CGAAGGCCTC GTCAAATCCG AAGCCTCGAC ACCCGCGGTT CCCGCCAGCA AATCGTCGAA GAAGAACATC CCGGTCACAA TCGTCGAAAC GCCGCCCACC ACGGACCCGA TCGACGAATC GAGCCAATAT CGCACCTGGC GAATCGAGAA TCGCTATCAG CCCGGCCAGG TGATCGAACT CCCGCTCAAG GCGATCAAAC CGAGCCCCTT CAACCCGCGG CACTTCTATT TGAAGTCGTC GATCGCGGAA CTGGCAGTCA ATCTGGCGAA GCAGGGTCAG CAACAAGCGA TCCACGTCAT CCCCGATTAC GACAACCCCG GCTCGTACTT CGTCAGCGAC GGCGGCCGCC GCGTCCGTGC ACTGAAGGAA GCGAACAAGG AATCGGTCAA GGCGATCGTC ATCGACCTGC CGATCGGCAT TCAAAGCTAC AAGCTCGGCT ACGACCTCAA CGTCCAACGT GATTCACAAA CGGTCTTCGA TAACGCAATC GTCTGGAAGC GCTTTCTCGA GGAAAAGTAT TTCCAGAGCC AGAAGGAATT GGCCGAGCAT CTCGGGCTCG ACGAATCGAC GGTCGCCGTT GCGCTCTCAA TCGGCAAGTT GCCCGAAGCC GTGATGCAGG AAATGGTTGC GCGCCCGGAT CGCTTCGGCT CGAACATGGC GTATCAGGTC GGCCGTTACC ACACCGCGCG CGGCACCGAC TCGACACTGC GTCTCATCAA CAAGATCCTG TCCGACGATC TCAGCACTCG TCAGGTCGCC GATATCGTGA AGGGAAGGGC GAGCGCGCAA GAAAGCCCGA AGCCCGCGAG TCGACAGCGG TATGCGCAGC GACATGAGAT CAAGCTGAAC GGCATGTCGG TCGGCGATCT GAAGTCGTAC GGCGAAGATC GTCTGGAGTT GCGGCTGCGC GGCCTGACGC GAGAAAAGCG GGATGAAATC CTGCAGCAGC TCGAACGGAT GCTGTTGTCG AATTAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA