ButhA.17622.a

Hydroxymethylglutaryl-coenzyme A reductase family protein (Uncharacterized protein)

CENTER ID: ButhA.17622.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.17622.a.B1.GE41319 full length 1 270
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.1670
UniProt: Q2T540

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLPTFDAGET RIAVAFPSFA NRLPLVRLGR QGLVFRARGA MPPVCGLPRS ATLYLDDKPL CTLRLVIREV ERCDDGAHDL TMQPSAANGD ALLWHALRTR CRHAHAPLAQ RAPGERATTA TQAAPRRHDS AKRAESGAPH AVGTGTCVRM TRSAAFRLAD RGDALFFADW LEYHFDELRA LAQGLSPRLH LNELERNLMG DEVDVRFVYD IDGHADRRML TDCARQACDW IAAEVRRRFD LPIAEQRFGA RKPRRATRAI RFRRSNSGRT
NT Sequence
atgctcccta cgttcgatgc cggcgaaacc cgcatcgccg tcgcgttccc atcattcgcc aatcgcctgc cgctcgtgcg gctcggccgg caggggctcg tatttcgcgc gcgcggcgcg atgccgcccg tctgcgggct gccgcgctcc gcaaccctgt atctcgacga caagccgctc tgcacgctgc ggctcgtgat tcgcgaagtc gagcgctgcg acgacggcgc gcacgatctg acaatgcagc cgtccgcggc gaacggcgac gcgctgctct ggcacgcgct gcgcacgcga tgccggcacg cgcacgcgcc gctcgcgcag cgcgcgccgg gcgagcgcgc gacaacggcg acgcaggccg cgccccgccg gcacgactcc gcgaagcgcg ccgaatcggg cgcgccgcat gcggtcggaa ccggcacctg cgtgcgcatg acgcgcagcg cggcgttccg cctcgcggat cgcggcgacg cgctcttttt cgccgattgg ctcgaatacc acttcgacga gttgcgcgcg ctcgcgcaag gcctgtcgcc gcggctgcat ctgaacgagc tcgagcggaa cctgatgggt gacgaagtcg acgttcgctt cgtctacgac atcgacggcc acgcggaccg ccggatgctc accgattgcg cgcgccaggc atgcgactgg attgcggcgg aagtgcgccg ccgcttcgat ctgccgatcg ccgagcagcg cttcggcgcg cgcaagccgc gccgcgcgac gcgcgctatt cgttttcgtc gaagtaatag tggccgaact tga
Details for ButhA.17622.a.B1.GE41319
HARVESTED ON: 3/31/2017
SEQUENCED ON: 4/10/2017
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 46
PERCENT COVERAGE: 74
Validated AA Sequence
MAHHHHHHML PTFDAGETRI AVAFPSFANR LPLVXXGRQG LVFRARGAMP PVCGLPRSGN PVSRRQAALH AAARDSRSRA LRRRRARSDN AAVRGERRRA ALARAAHAMP ARARAARAAR AGRARDNGDA GRAPPARLRE ARRIGRAACG RNRHLRAHDA QRGVPPRGSR RRALFRRLAR IPLRRVARAR ARPVAAAASE RARAEPDG
Validated NT Sequence
cncgcggcgc ggcttnnngc gcgccgaagc gctgctcggc gatcggcaga tcgaagcggc ggcgcacttc cgcccgcaat ccagtcgcat gcctggcgcg cgcaatcggt gagcatccgg cggtccgcgt ggccgtcgat gtcgtagacg aagcgaacgt cgacttcgtc acccatcagg ttccgctcga gctcgttcag atgcagccgc ggcgacaggc cttgcgcgag cgcgcgcaac tcgtcgaagt ggtattcgag ccaatcggcg aaaaagagcg cgtcgccgcg atccgcgagg cggaacgccg cgctgcgcgt catgcgcacg caggtgccgg ttccgaccgc atgcggcgcg cccgattcgg cgcgcttcgc ggagtcgtgc cggcggggcg cggcctgcgt cgccgttgtc gcgcgctcgc ccggcgcgcg ctgcgcgagc ggcgcgtgcg cgtgccggca tcgcgtgcgc agcgcgtgcc agagcagcgc gtcgccgttc gccgcggacg gctgcattgt cagatcgtgc gcgccgtcgt cgcagcgctc gacttcgcga atcacgagcc gcagcgtgca gagcggcttg tcgtcgagat acagggttgc cggagcgcgg cagcccgcag acgggcggca tcgcgccgcg cgcgcgaaat acgagcccct gccggccgnn nnncacgagc ggcaggcgat tggcgaatga tgggaacgcg acggcgatgc gggtttcgcc ggcatcgaac gtagggagca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHML PTFDAGETRI AVAFPSFANR LPLVRLGRQG LVFRARGAMP PVCGLPRSAT LYLDDKPLCT LRLVIREVER CDDGAHDLTM QPSAANGDAL LWHALRTRCR HAHAPLAQRA PGERATTATQ AAPRRHDSAK RAESGAPHAV GTGTCVRMTR SAAFRLADRG DALFFADWLE YHFDELRALA QGLSPRLHLN ELERNLMGDE VDVRFVYDID GHADRRMLTD CARQACDWIA AEVRRRFDLP IAEQRFGARK PRRATRAIRF RRSNSGRT
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctcc ctacgttcga tgccggcgaa acccgcatcg ccgtcgcgtt cccatcattc gccaatcgcc tgccgctcgt gcggctcggc cggcaggggc tcgtatttcg cgcgcgcggc gcgatgccgc ccgtctgcgg gctgccgcgc tccgcaaccc tgtatctcga cgacaagccg ctctgcacgc tgcggctcgt gattcgcgaa gtcgagcgct gcgacgacgg cgcgcacgat ctgacaatgc agccgtccgc ggcgaacggc gacgcgctgc tctggcacgc gctgcgcacg cgatgccggc acgcgcacgc gccgctcgcg cagcgcgcgc cgggcgagcg cgcgacaacg gcgacgcagg ccgcgccccg ccggcacgac tccgcgaagc gcgccgaatc gggcgcgccg catgcggtcg gaaccggcac ctgcgtgcgc atgacgcgca gcgcggcgtt ccgcctcgcg gatcgcggcg acgcgctctt tttcgccgat tggctcgaat accacttcga cgagttgcgc gcgctcgcgc aaggcctgtc gccgcggctg catctgaacg agctcgagcg gaacctgatg ggtgacgaag tcgacgttcg cttcgtctac gacatcgacg gccacgcgga ccgccggatg ctcaccgatt gcgcgcgcca ggcatgcgac tggattgcgg cggaagtgcg ccgccgcttc gatctgccga tcgccgagca gcgcttcggc gcgcgcaagc cgcgccgcgc gacgcgcgct attcgttttc gtcgaagtaa tagtggccga acttgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat