ButhA.17449.a

YdjC-like family protein

CENTER ID: ButhA.17449.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.17449.a.B1.GE35973 full length 1 295
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.4557
RefSeq: YP_442300.1
UniProt: Q2SXP9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAACDAAPRA LIFTADDFGL HERVNAAVER AHRDGVLSAA SLMVGASAAR DAVERAKRLP SLAVGLHIVL ADGPATLPAR EIPALVGPDG RFRDAMAKDG CRFFFLPHVR AQLRREIRAQ FEAFAATGLA LDHVNTHKHF HLHPTVLSMI VDIGREFGLA AMRLPYEPST PFWLKPWIAL VRARLDRAGI AHNDYVVGIE RTGAMDEAAL LAALAKLPAG VGEIYSHPAE AGDGPITPSM RDYRPADELA ALLSPRVAAA VQAAGVACGG FADVFGGRAR ARTPRGARIS GAQPS
NT Sequence
gtggcggctt gcgacgcggc gccgcgcgcg ctgatcttca cggcggacga cttcggcctg catgagcggg tcaacgcggc cgtcgagcgt gcgcaccgag acggcgtgct ctcggccgcg agcctgatgg tcggcgcgag cgccgcgcgc gacgcggtcg agcgcgcgaa gcgcctgccc tcgctggccg tcggcctgca catcgtgctc gccgacgggc ccgcgacgtt gcccgcgcgc gagatcccgg cgctcgtcgg acccgacggg cgttttcgcg acgcgatggc gaaggacggc tgccgcttct tcttcctgcc gcacgtgcgg gcgcaactgc gccgcgagat ccgcgcgcag ttcgaggcgt tcgcggcgac cgggctcgcg ctcgatcacg tgaacacgca caagcatttc catctgcatc cgaccgtgct gtcgatgatc gtcgatatcg gccgcgagtt cgggcttgcc gcgatgcggc taccgtacga gccgtcgacg ccgttctggc tcaagccgtg gatcgcgctc gtgcgcgcgc ggctcgaccg cgcgggcatc gcgcacaacg actacgtcgt cggcatcgag cgcacgggcg cgatggacga ggcggcgctg ctcgccgcgc tcgcgaagct gccggcgggc gtcggcgaaa tctacagcca tccggcggag gcgggcgacg gcccgatcac gccgtcgatg cgcgattatc ggccggccga cgaactcgcc gcgctgctgt cgccgcgcgt cgcggccgcg gtgcaggcgg cgggcgtcgc gtgcggcggc ttcgccgacg tgttcggcgg ccgcgcgcgt gcgcgaacgc cgcgcggtgc gcggatcagc ggggcgcagc cgtcatga
Details for ButhA.17449.a.B1.GE35973
HARVESTED ON: 8/28/2012
SEQUENCED ON: 8/31/2012
EXPECTED MW: 32kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMV GACDAAPRAL IFTADDFGLH ERVNAAVERA HRDGVLSAAS LMVGASAARD AVERAKRLPS LAVGLHIVLA DGPATLPARE IPALVGPDGR FRDAMAKDGC RFFFLPHVRA QLRREIRAQF EAFAATGLAL DHVNTHKHFH LHPTVLSMIV DIGREFGLAA MRLPYEPSTP FWLKPWIALV RARLDRAGIA HNDYVVGIER TGAMDEAALL AALAKLPAGV GEIYSHPAXA GDGPITPSMR DYRPADELAA LLSPRVAAAV XAAGVACGGF ADVFGGRARA RTPRGARISG AXPS
Validated NT Sequence
attttgtttn ntttaagaag gaganatacc atggctcacc accaccacca ccatatggtg ggggcttgcg acgcggcgcc gcgcgcgctg atcttcacgg cggacgactt cggcctgcat gagcgggtca acgcggccgt cgagcgtgcg caccgagacg gcgtgctctc ggccgcgagc ctgatggtcg gcgcgagcgc cgcgcgcgac gcggtcgagc gcgcgaagcg cctgccctcg ctggccgtcg gcctgcacat cgtgctcgcc gacgggcccg cgacgttgcc cgcgcgcgag atcccggcgc tcgtcggacc cgacgggcgt tttcgcgacg cgatggcgaa ggacggctgc cgcttcttct tcctgccgca cgtgcgggcg caactgcgcc gcgagatccg cgcgcagttc gaggcgttcg cggcgaccgg gctcgcgctc gatcacgtga acacgcacaa gcatttccat ctgcatccga ccgtgctgtc gatgatcgtc gatatcggcc gcgagttcgg gcttgccgcg atgcggctac cgtacgagcc gtcgacgccg ttctggctca agccgtggat cgcgctcgtg cgcgcgcggc tcgaccgcgc gggcatcgcg cacaacgact acgtcgtcgg catcgagcgc acgggcgcga tggacgaggc ggcgctgctc gccgcgctcg cgaagctgcc ggcgggcgtc ggcgaaatct acagccatcc ggcggangcg ggcgacggcc cgatcacgcc gtcnatgcgc gattatcggc cggccgacga actcgccgcg ctgctgtcgc cgcgcgtcgc ggccgcggtg cnngcggcgg gcgtcgcgtg cggcggcttc gccgacgtgt tcggcggccg cgcgcgtgcg cgaacgccgc gcggngcgcg gatcagcggg gcgcanccgt cataagtgag taananagnn nncnnnnnnn nnnnnncccc
Expected Protein Sequence
MAHHHHHHMV AACDAAPRAL IFTADDFGLH ERVNAAVERA HRDGVLSAAS LMVGASAARD AVERAKRLPS LAVGLHIVLA DGPATLPARE IPALVGPDGR FRDAMAKDGC RFFFLPHVRA QLRREIRAQF EAFAATGLAL DHVNTHKHFH LHPTVLSMIV DIGREFGLAA MRLPYEPSTP FWLKPWIALV RARLDRAGIA HNDYVVGIER TGAMDEAALL AALAKLPAGV GEIYSHPAEA GDGPITPSMR DYRPADELAA LLSPRVAAAV QAAGVACGGF ADVFGGRARA RTPRGARISG AQPS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtgg cggcttgcga cgcggcgccg cgcgcgctga tcttcacggc ggacgacttc ggcctgcatg agcgggtcaa cgcggccgtc gagcgtgcgc accgagacgg cgtgctctcg gccgcgagcc tgatggtcgg cgcgagcgcc gcgcgcgacg cggtcgagcg cgcgaagcgc ctgccctcgc tggccgtcgg cctgcacatc gtgctcgccg acgggcccgc gacgttgccc gcgcgcgaga tcccggcgct cgtcggaccc gacgggcgtt ttcgcgacgc gatggcgaag gacggctgcc gcttcttctt cctgccgcac gtgcgggcgc aactgcgccg cgagatccgc gcgcagttcg aggcgttcgc ggcgaccggg ctcgcgctcg atcacgtgaa cacgcacaag catttccatc tgcatccgac cgtgctgtcg atgatcgtcg atatcggccg cgagttcggg cttgccgcga tgcggctacc gtacgagccg tcgacgccgt tctggctcaa gccgtggatc gcgctcgtgc gcgcgcggct cgaccgcgcg ggcatcgcgc acaacgacta cgtcgtcggc atcgagcgca cgggcgcgat ggacgaggcg gcgctgctcg ccgcgctcgc gaagctgccg gcgggcgtcg gcgaaatcta cagccatccg gcggaggcgg gcgacggccc gatcacgccg tcgatgcgcg attatcggcc ggccgacgaa ctcgccgcgc tgctgtcgcc gcgcgtcgcg gccgcggtgc aggcggcggg cgtcgcgtgc ggcggcttcg ccgacgtgtt cggcggccgc gcgcgtgcgc gaacgccgcg cggtgcgcgg atcagcgggg cgcagccgtc atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat