SSGCID: Target Page

ButhA.17401.a

Putative uncharacterized protein (BTH_I2190)

CENTER ID:	ButhA.17401.a
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.17401.a.A1.GE32781	full length		1	351

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|271848.6.peg.5009
RefSeq:	YP_442711.1
UniProt:	Q2SWI8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKAKKVLILG VNGFIGHHLS KRILETTDWE VFGMDMQTDR LGDLVKHERM HFFEGDITIN KEWVEYHVKK CDVILPLVAI ATPATYVQQP LRVFELDFEA NLPIVRSAVK YKKHLVFPST SEVYGMCADE QFDPDASALT YGPINKPRWI YACSKQLMDR VIWGYGMEGL NFTLFRPFNW IGPGLDSIYT PKEGSSRVVT QFLGHIVRGE NISLVDGGSQ KRAFTYVDDG ISALMKIIEN PNGVATGKIY NIGNPNNNFS VRELANKMLE LAAEFPEYTD SAKRVKLVET TSGAYYGNGY QDVQNRVPKI ENTMRELGWA PQFTFDDALR QIFEAYRGHV ADARALVEQQ G

NT Sequence

atgaaagcta aaaaagtcct gatcctgggt gtgaacggct tcatcggcca tcacctgtcc aagcgcattc ttgaaaccac cgattgggaa gtgttcggca tggacatgca gaccgatcgg ctcggcgatc tcgtcaagca cgagcggatg catttcttcg aaggcgacat cacgatcaac aaggagtggg tcgagtatca cgtgaagaag tgcgacgtga tcctgccgct cgtcgcgatc gcgacgcccg ccacctacgt ccagcagccg ctgcgcgtgt tcgagctcga cttcgaggcg aacctgccca tcgtgcgctc ggccgtcaag tacaagaagc atctcgtgtt tccgtcgacg tccgaggtgt acgggatgtg cgcggacgag cagttcgatc cggacgcgtc cgccctcacc tacggcccga tcaacaagcc gcgctggatc tacgcgtgct ccaagcagtt gatggaccgc gtgatctggg gctacggaat ggaaggcctg aacttcacgc tgttccgccc gttcaactgg atcggcccgg gcctcgactc gatctacacg ccgaaggaag gcagctcgcg cgtcgtcacg caattcctcg gccacatcgt gcgcggcgag aacatcagcc tcgtcgacgg cggctcgcag aagcgcgcgt tcacgtacgt cgacgacggc atcagcgcgc tgatgaagat catcgagaat ccgaacggcg tcgcgacggg caagatctac aacatcggga atccgaacaa taacttctcg gtgcgcgagc tcgcgaacaa gatgctcgag ctcgcggcgg aattccccga gtacacggat tcggccaagc gcgtgaagct cgtcgaaacc acgtcgggcg cgtactacgg caacggctat caggatgtgc agaaccgcgt gccgaaaatc gagaacacga tgcgcgagct cggctgggcg ccgcagttca cgttcgacga cgcactgcgc cagatcttcg aggcgtaccg cggccacgtc gccgacgcgc gcgcgctcgt cgagcagcaa ggctga

Details for ButhA.17401.a.A1.GE32781

HARVESTED ON:	6/20/2011
SEQUENCED ON:	7/14/2011
EXPECTED MW:	42kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMKAKKVLIL GVNGFIGHHL SKRILETTDW EVFGMDMQTD RLGDLVKHER MHFFEGDITI NKEWVEYHVK KCDVILPLVA IATPATYVQQ PLRVFELDFE ANLPIVRSAV KYKKHLVFPS TSEVYGMCAD EQFDPDASAL TYGPINKPRW IYACSKQLMD RVIWGYGMEG LNFTLFRPFN WIGPGLDSIY TPKEGSSRVV TQFLGHIVRG ENISLVDGGS QKRAFTYVDD GISALMKIIE NPNGVATGKI YNIGNPNNNF SVRELANKML ELAAEFPEYT DSAKRVKLVE TTSGAYYGNG YQDVQNRVPK IENTMRELGW APQFTFDDAL RQIFEAYRGH VADARALVE

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaaag ctaaaaaagt cctgatcctg ggtgtgaacg gcttcatcgg ccatcacctg tccaagcgca ttcttgaaac caccgattgg gaagtgttcg gcatggacat gcagaccgat cggctcggcg atctcgtcaa gcacgagcgg atgcatttct tcgaaggcga catcacgatc aacaaggagt gggtcgagta tcacgtgaag aagtgcgacg tgatcctgcc gctcgtcgcg atcgcgacgc ccgccaccta cgtccagcag ccgctgcgcg tgttcgagct cgacttcgag gcgaacctgc ccatcgtgcg ctcggccgtc aagtacaaga agcatctcgt gtttccgtcg acgtccgagg tgtacgggat gtgcgcggac gagcagttcg atccggacgc gtccgccctc acctacggcc cgatcaacaa gccgcgctgg atctacgcgt gctccaagca gttgatggac cgcgtgatct ggggctacgg aatggaaggc ctgaacttca cgctgttccg cccgttcaac tggatcggcc cgggcctcga ctcgatctac acgccgaagg aaggcagctc gcgcgtcgtc acgcaattcc tcggccacat cgtgcgcggc gagaacatca gcctcgtcga cggcggctcg cagaagcgcg cgttcacgta cgtcgacgac ggcatcagcg cgctgatgaa gatcatcgag aatccgaacg gcgtcgcgac gggcaagatc tacaacatcg ggaatccgaa caataacttc tcggtgcgcg agctcgcgaa caagatgctc gagctcgcgg cggaattccc cgagtacacg gattcggcca agcgcgtgaa gctcgtcgaa accacgtcgg gcgcgtacta cggcaacggc tatcaggatg tgcagaaccg cgtgccgaaa atcgagaaca cgatgcgcga gctcggctgg gcgccgcagt tcacgttcga cgacgcactg cgccagatct tcgaggcgta ccgcggccac gtcgccgacg cgcgcgcgct cgtcgagcag

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaagctaaa aaagtcctga tcctgggtgt gaacggcttc atcggccatc acctgtccaa gcgcattctt gaaaccaccg attgggaagt gttcggcatg gacatgcaga ccgatcggct cggcgatctc gtcaagcacg agcggatgca tttcttcgaa ggcgacatca cgatcaacaa ggagtgggtc gagtatcacg tgaagaagtg cgacgtgatc ctgccgctcg tcgcgatcgc gacgcccgcc acctacgtcc agcagccgct gcgcgtgttc gagctcgact tcgaggcgaa cctgcccatc gtgcgctcgg ccgtcaagta caagaagcat ctcgtgtttc cgtcgacgtc cgaggtgtac gggatgtgcg cggacgagca gttcgatccg gacgcgtccg ccctcaccta cggcccgatc aacaagccgc gctggatcta cgcgtgctcc aagcagttga tggaccgcgt gatctggggc tacggaatgg aaggcctgaa cttcacgctg ttccgcccgt tcaactggat cggcccgggc ctcgactcga tctacacgcc gaaggaaggc agctcgcgcg tcgtcacgca attcctcggc cacatcgtgc gcggcgagaa catcagcctc gtcgacggcg gctcgcagaa gcgcgcgttc acgtacgtcg acgacggcat cagcgcgctg atgaagatca tcgagaatcc gaacggcgtc gcgacgggca agatctacaa catcgggaat ccgaacaata acttctcggt gcgcgagctc gcgaacaaga tgctcgagct cgcggcggaa ttccccgagt acacggattc ggccaagcgc gtgaagctcg tcgaaaccac gtcgggcgcg tactacggca acggctatca ggatgtgcag aaccgcgtgc cgaaaatcga gaacacgatg cgcgagctcg gctgggcgcc gcagttcacg ttcgacgacg cactgcgcca gatcttcgag gcgtaccgcg gccacgtcgc cgacgcgcgc gcgctcgtcg agcagcaagg ctaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa