ButhA.17369.c
ATP synthase subunit beta 1 (EC 3.6.3.14) (ATP synthase F1 sector subunit beta 1) (F-ATPase subunit beta 1) (BTH_I3308) (atpD1)
| CENTER ID: | ButhA.17369.c |
| ORGANISM: | Burkholderia thailandensis E264 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.17369.c.A1.GE32778 | full length | 1 | 464 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ButhA.17369.c.A1.PW35952 | full length | 1 | 464 |
Structures
4Q4L
DEPOSITED: 4/14/2014
DETERMINATION: XRay
CLONE: ButhA.17369.c.A1.GE32778
PROTEIN: ButhA.17369.c.A1.PW35952
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_127099 |
| PATRIC ID: | fig|271848.6.peg.6195 |
| RefSeq: | YP_443800.1 |
| UniProt: | Q2STE9 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSTAALVEGK IVQCIGAVID VEFPRESMPK IYDALILEGS ELTLEVQQQL GDGVVRTICL GASDGLRRGV VVKNTGNPIS VPVGKPTLGR IMDVLGRPID EAGPIESENK RSIHQKAPAF DELSPSTELL ETGIKVIDLI CPFAKGGKVG LFGGAGVGKT VNMMELINNI AKEHGGYSVF AGVGERTREG NDFYHEMKDS NVLDKVALVY GQMNEPPGNR LRVALTGLTM AEHFRDEGLD VLFFVDNIYR FTLAGTEVSA LLGRMPSAVG YQPTLAEEMG KLQERITSTK KGSITSVQAV YVPADDLTDP SPATTFGHLD ATVVLSRDIA SLGIYPAVDP LDSTSRQIDP NVIGEEHYSI TRRVQQTLQR YKELRDIIAI LGMDELSPED KLSVARARKI QRFLSQPFHV AEVFTGSPGK YVPLKETIRG FKMIVDGECD HLPEQAFYMV GTIDEAFEKA KKIQ
NT Sequence
atgagtactg ctgctttggt agaaggcaag atcgtacagt gcatcggcgc cgttatcgac gtggaatttc cgcgcgagag catgccgaag atctacgacg cgctcattct ggaaggctcg gaactgacgc tcgaagtcca gcagcagctg ggcgacggcg tggtccgcac catctgtctg ggggcatccg acggcctgcg ccgcggcgtc gtggtgaaga atacgggcaa tccgatttcg gtgccggtcg gcaagccgac cctcggccgc atcatggacg tgctcggccg cccgatcgac gaagcgggcc cgatcgagag cgaaaacaag cgctcgatcc accagaaggc gccggcgttc gacgagctgt cgccgtcgac cgaactgctt gaaacgggca tcaaggtcat cgacctgatc tgcccgttcg cgaagggcgg caaggttggc ctgttcggcg gtgctggcgt gggcaagacc gtcaacatga tggagctcat caacaacatc gcgaaggaac acggcggtta ctccgtgttc gcgggcgtgg gcgagcgtac ccgtgaaggg aacgacttct atcacgaaat gaaggactcg aacgttctcg acaaggtcgc gctggtgtac ggccagatga acgagccgcc gggcaaccgt ctgcgcgtcg cgctgacggg cctcacgatg gccgagcact tccgtgacga aggcctcgac gtgctgttct tcgtcgacaa catctaccgt ttcacgctgg ccggtaccga agtgtcggca ctgctcggcc gtatgccgtc ggccgtgggc tatcagccga cgctggccga ggaaatgggc aagctgcagg aacgcatcac gtcgaccaag aagggctcga tcacgtcggt tcaggccgtg tacgtccctg cggacgactt gaccgacccg tcgccggcca cgaccttcgg ccacctggac gcaaccgtcg ttctgtcgcg tgacatcgct tcgctgggca tctacccggc ggtcgacccg ctcgactcga cgtcgcgcca gatcgacccg aacgtgatcg gcgaagagca ctactcgatc acgcgtcgcg ttcagcagac gctgcagcgc tacaaggaac tgcgcgacat catcgcgatt ctgggcatgg acgaactgtc gccggaagac aagctgtcgg tcgcacgtgc gcgtaagatc cagcgtttcc tgtcgcagcc gttccacgtc gccgaagtgt tcacgggctc gccgggcaag tacgtgccgc tgaaggaaac gatccgcggc ttcaagatga tcgtcgacgg cgagtgcgac cacctgccgg aacaggcgtt ctacatggtc ggcacgatcg acgaagcctt cgagaaggcc aagaagatcc agtaa
Details for ButhA.17369.c.A1.GE32778
| HARVESTED ON: | 6/20/2011 |
| SEQUENCED ON: | 7/14/2011 |
| EXPECTED MW: | 52kDa |
| OBSERVED MW: | 50kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Validated AA Sequence
MGTLEAQTQG PGSMSTAALV EGKIVQCIGA VIDVEFPRES MPKIYDALIL EGSELTLEVQ QQLGDGVVRT ICLGASDGLR RGVVVKNTGN PISVPVGKPT LGRIMDVLGR PIDEAGPIES ENKRSIHQKA PAFDELSPST ELLETGIKVI DLICPFAKGG KVGLFGGAGV GKTVNMMELI NNIAKEHGGY SVFAGVGERT REGNDFYHEM KDSNVLDKVA LVYGQMNEPP GNRLRVALTG LTMAEHFRDE GLDVLFFVDN IYRFTLAGTE VSALLGRMPS AVGYQPTLAE EMGKLQERIT STKKGSITSV QAVYVPADDL TDPSPATTFG HLDATVVLSR DIASLGIYPA VDPLDSTSRQ IDPNVIGEEH YSITRRVQQT LQRYKELRDI IAILGMDELS PEDKLSVARA RKIQRFLSQP FHVAEVFTGS PGKYVPLKET IRGFKMIVDG ECDHLPEQAF YMVGTIDEAF EKAKKIQ
Validated NT Sequence
ttattactgg atcttcttgg ccttctcgaa ggcttcgtcg atcgtgccga ccatgtagaa cgcctgttcc ggcaggtggt cgcactcgcc gtcgacgatc atcttgaagc cgcggatcgt ttccttcagc ggcacgtact tgcccggcga gcccgtgaac acttcggcga cgtggaacgg ctgcgacagg aaacgctgga tcttacgcgc acgtgcgacc gacagcttgt cttccggcga cagttcgtcc atgcccagaa tcgcgatgat gtcgcgcagt tccttgtagc gctgcagcgt ctgctgaacg cgacgcgtga tcgagtagtg ctcttcgccg atcacgttcg ggtcgatctg gcgcgacgtc gagtcgagcg ggtcgaccgc cgggtagatg cccagcgaag cgatgtcacg cgacagaacg acggttgcgt ccaggtggcc gaaggtcgtg gccggcgacg ggtcggtcaa gtcgtccgca gggacgtaca cggcctgaac cgacgtgatc gagcccttct tggtcgacgt gatgcgttcc tgcagcttgc ccatttcctc ggccagcgtc ggctgatagc ccacggccga cggcatacgg ccgagcagtg ccgacacttc ggtaccggcc agcgtgaaac ggtagatgtt gtcgacgaag aacagcacgt cgaggccttc gtcacggaag tgctcggcca tcgtgaggcc cgtcagcgcg acgcgcagac ggttgcccgg cggctcgttc atctggccgt acaccagcgc gaccttgtcg agaacgttcg agtccttcat ttcgtgatag aagtcgttcc cttcacgggt acgctcgccc acgcccgcga acacggagta accgccgtgt tccttcgcga tgttgttgat gagctccatc atgttgacgg tcttgcccac gccagcaccg ccgaacaggc caaccttgcc gcccttcgcg aacgggcaga tcaggtcgat gaccttgatg cccgtttcaa gcagttcggt cgacggcgac agctcgtcga acgccggcgc cttctggtgg atcgagcgct tgttttcgct ctcgatcggg cccgcttcgt cgatcgggcg gccgagcacg tccatgatgc ggccgagggt cggcttgccg accggcaccg aaatcggatt gcccgtattc ttcaccacga cgccgcggcg caggccgtcg gatgccccca gacagatggt gcggaccacg ccgtcgccca gctgctgctg gacttcgagc gtcagttccg agccttccag aatgagcgcg tcgtagatct tcggcatgct ctcgcgcgga aattccacgt cgataacggc gccgatgcac tgtacgatct tgccttctac caaagcagca gtactcatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSTAALVEG KIVQCIGAVI DVEFPRESMP KIYDALILEG SELTLEVQQQ LGDGVVRTIC LGASDGLRRG VVVKNTGNPI SVPVGKPTLG RIMDVLGRPI DEAGPIESEN KRSIHQKAPA FDELSPSTEL LETGIKVIDL ICPFAKGGKV GLFGGAGVGK TVNMMELINN IAKEHGGYSV FAGVGERTRE GNDFYHEMKD SNVLDKVALV YGQMNEPPGN RLRVALTGLT MAEHFRDEGL DVLFFVDNIY RFTLAGTEVS ALLGRMPSAV GYQPTLAEEM GKLQERITST KKGSITSVQA VYVPADDLTD PSPATTFGHL DATVVLSRDI ASLGIYPAVD PLDSTSRQID PNVIGEEHYS ITRRVQQTLQ RYKELRDIIA ILGMDELSPE DKLSVARARK IQRFLSQPFH VAEVFTGSPG KYVPLKETIR GFKMIVDGEC DHLPEQAFYM VGTIDEAFEK AKKIQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagtactgct gctttggtag aaggcaagat cgtacagtgc atcggcgccg ttatcgacgt ggaatttccg cgcgagagca tgccgaagat ctacgacgcg ctcattctgg aaggctcgga actgacgctc gaagtccagc agcagctggg cgacggcgtg gtccgcacca tctgtctggg ggcatccgac ggcctgcgcc gcggcgtcgt ggtgaagaat acgggcaatc cgatttcggt gccggtcggc aagccgaccc tcggccgcat catggacgtg ctcggccgcc cgatcgacga agcgggcccg atcgagagcg aaaacaagcg ctcgatccac cagaaggcgc cggcgttcga cgagctgtcg ccgtcgaccg aactgcttga aacgggcatc aaggtcatcg acctgatctg cccgttcgcg aagggcggca aggttggcct gttcggcggt gctggcgtgg gcaagaccgt caacatgatg gagctcatca acaacatcgc gaaggaacac ggcggttact ccgtgttcgc gggcgtgggc gagcgtaccc gtgaagggaa cgacttctat cacgaaatga aggactcgaa cgttctcgac aaggtcgcgc tggtgtacgg ccagatgaac gagccgccgg gcaaccgtct gcgcgtcgcg ctgacgggcc tcacgatggc cgagcacttc cgtgacgaag gcctcgacgt gctgttcttc gtcgacaaca tctaccgttt cacgctggcc ggtaccgaag tgtcggcact gctcggccgt atgccgtcgg ccgtgggcta tcagccgacg ctggccgagg aaatgggcaa gctgcaggaa cgcatcacgt cgaccaagaa gggctcgatc acgtcggttc aggccgtgta cgtccctgcg gacgacttga ccgacccgtc gccggccacg accttcggcc acctggacgc aaccgtcgtt ctgtcgcgtg acatcgcttc gctgggcatc tacccggcgg tcgacccgct cgactcgacg tcgcgccaga tcgacccgaa cgtgatcggc gaagagcact actcgatcac gcgtcgcgtt cagcagacgc tgcagcgcta caaggaactg cgcgacatca tcgcgattct gggcatggac gaactgtcgc cggaagacaa gctgtcggtc gcacgtgcgc gtaagatcca gcgtttcctg tcgcagccgt tccacgtcgc cgaagtgttc acgggctcgc cgggcaagta cgtgccgctg aaggaaacga tccgcggctt caagatgatc gtcgacggcg agtgcgacca cctgccggaa caggcgttct acatggtcgg cacgatcgac gaagccttcg agaaggccaa gaagatccag taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for ButhA.17369.c.A1.PW35952
| PURIFICATION DATe: | 3/31/2012 |
| CONCENTRATION: | 29.27mg/ml |
| OBSERVED MW: | 56kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 12 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMSTAALV EGKIVQCIGA VIDVEFPRES MPKIYDALIL EGSELTLEVQ QQLGDGVVRT ICLGASDGLR RGVVVKNTGN PISVPVGKPT LGRIMDVLGR PIDEAGPIES ENKRSIHQKA PAFDELSPST ELLETGIKVI DLICPFAKGG KVGLFGGAGV GKTVNMMELI NNIAKEHGGY SVFAGVGERT REGNDFYHEM KDSNVLDKVA LVYGQMNEPP GNRLRVALTG LTMAEHFRDE GLDVLFFVDN IYRFTLAGTE VSALLGRMPS AVGYQPTLAE EMGKLQERIT STKKGSITSV QAVYVPADDL TDPSPATTFG HLDATVVLSR DIASLGIYPA VDPLDSTSRQ IDPNVIGEEH YSITRRVQQT LQRYKELRDI IAILGMDELS PEDKLSVARA RKIQRFLSQP FHVAEVFTGS PGKYVPLKET IRGFKMIVDG ECDHLPEQAF YMVGTIDEAF EKAKKIQ
Validated NT Sequence
ttattactgg atcttcttgg ccttctcgaa ggcttcgtcg atcgtgccga ccatgtagaa cgcctgttcc ggcaggtggt cgcactcgcc gtcgacgatc atcttgaagc cgcggatcgt ttccttcagc ggcacgtact tgcccggcga gcccgtgaac acttcggcga cgtggaacgg ctgcgacagg aaacgctgga tcttacgcgc acgtgcgacc gacagcttgt cttccggcga cagttcgtcc atgcccagaa tcgcgatgat gtcgcgcagt tccttgtagc gctgcagcgt ctgctgaacg cgacgcgtga tcgagtagtg ctcttcgccg atcacgttcg ggtcgatctg gcgcgacgtc gagtcgagcg ggtcgaccgc cgggtagatg cccagcgaag cgatgtcacg cgacagaacg acggttgcgt ccaggtggcc gaaggtcgtg gccggcgacg ggtcggtcaa gtcgtccgca gggacgtaca cggcctgaac cgacgtgatc gagcccttct tggtcgacgt gatgcgttcc tgcagcttgc ccatttcctc ggccagcgtc ggctgatagc ccacggccga cggcatacgg ccgagcagtg ccgacacttc ggtaccggcc agcgtgaaac ggtagatgtt gtcgacgaag aacagcacgt cgaggccttc gtcacggaag tgctcggcca tcgtgaggcc cgtcagcgcg acgcgcagac ggttgcccgg cggctcgttc atctggccgt acaccagcgc gaccttgtcg agaacgttcg agtccttcat ttcgtgatag aagtcgttcc cttcacgggt acgctcgccc acgcccgcga acacggagta accgccgtgt tccttcgcga tgttgttgat gagctccatc atgttgacgg tcttgcccac gccagcaccg ccgaacaggc caaccttgcc gcccttcgcg aacgggcaga tcaggtcgat gaccttgatg cccgtttcaa gcagttcggt cgacggcgac agctcgtcga acgccggcgc cttctggtgg atcgagcgct tgttttcgct ctcgatcggg cccgcttcgt cgatcgggcg gccgagcacg tccatgatgc ggccgagggt cggcttgccg accggcaccg aaatcggatt gcccgtattc ttcaccacga cgccgcggcg caggccgtcg gatgccccca gacagatggt gcggaccacg ccgtcgccca gctgctgctg gacttcgagc gtcagttccg agccttccag aatgagcgcg tcgtagatct tcggcatgct ctcgcgcgga aattccacgt cgataacggc gccgatgcac tgtacgatct tgccttctac caaagcagca gtactcatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSTAALVEG KIVQCIGAVI DVEFPRESMP KIYDALILEG SELTLEVQQQ LGDGVVRTIC LGASDGLRRG VVVKNTGNPI SVPVGKPTLG RIMDVLGRPI DEAGPIESEN KRSIHQKAPA FDELSPSTEL LETGIKVIDL ICPFAKGGKV GLFGGAGVGK TVNMMELINN IAKEHGGYSV FAGVGERTRE GNDFYHEMKD SNVLDKVALV YGQMNEPPGN RLRVALTGLT MAEHFRDEGL DVLFFVDNIY RFTLAGTEVS ALLGRMPSAV GYQPTLAEEM GKLQERITST KKGSITSVQA VYVPADDLTD PSPATTFGHL DATVVLSRDI ASLGIYPAVD PLDSTSRQID PNVIGEEHYS ITRRVQQTLQ RYKELRDIIA ILGMDELSPE DKLSVARARK IQRFLSQPFH VAEVFTGSPG KYVPLKETIR GFKMIVDGEC DHLPEQAFYM VGTIDEAFEK AKKIQ
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGTACTGCT GCTTTGGTAG AAGGCAAGAT CGTACAGTGC ATCGGCGCCG TTATCGACGT GGAATTTCCG CGCGAGAGCA TGCCGAAGAT CTACGACGCG CTCATTCTGG AAGGCTCGGA ACTGACGCTC GAAGTCCAGC AGCAGCTGGG CGACGGCGTG GTCCGCACCA TCTGTCTGGG GGCATCCGAC GGCCTGCGCC GCGGCGTCGT GGTGAAGAAT ACGGGCAATC CGATTTCGGT GCCGGTCGGC AAGCCGACCC TCGGCCGCAT CATGGACGTG CTCGGCCGCC CGATCGACGA AGCGGGCCCG ATCGAGAGCG AAAACAAGCG CTCGATCCAC CAGAAGGCGC CGGCGTTCGA CGAGCTGTCG CCGTCGACCG AACTGCTTGA AACGGGCATC AAGGTCATCG ACCTGATCTG CCCGTTCGCG AAGGGCGGCA AGGTTGGCCT GTTCGGCGGT GCTGGCGTGG GCAAGACCGT CAACATGATG GAGCTCATCA ACAACATCGC GAAGGAACAC GGCGGTTACT CCGTGTTCGC GGGCGTGGGC GAGCGTACCC GTGAAGGGAA CGACTTCTAT CACGAAATGA AGGACTCGAA CGTTCTCGAC AAGGTCGCGC TGGTGTACGG CCAGATGAAC GAGCCGCCGG GCAACCGTCT GCGCGTCGCG CTGACGGGCC TCACGATGGC CGAGCACTTC CGTGACGAAG GCCTCGACGT GCTGTTCTTC GTCGACAACA TCTACCGTTT CACGCTGGCC GGTACCGAAG TGTCGGCACT GCTCGGCCGT ATGCCGTCGG CCGTGGGCTA TCAGCCGACG CTGGCCGAGG AAATGGGCAA GCTGCAGGAA CGCATCACGT CGACCAAGAA GGGCTCGATC ACGTCGGTTC AGGCCGTGTA CGTCCCTGCG GACGACTTGA CCGACCCGTC GCCGGCCACG ACCTTCGGCC ACCTGGACGC AACCGTCGTT CTGTCGCGTG ACATCGCTTC GCTGGGCATC TACCCGGCGG TCGACCCGCT CGACTCGACG TCGCGCCAGA TCGACCCGAA CGTGATCGGC GAAGAGCACT ACTCGATCAC GCGTCGCGTT CAGCAGACGC TGCAGCGCTA CAAGGAACTG CGCGACATCA TCGCGATTCT GGGCATGGAC GAACTGTCGC CGGAAGACAA GCTGTCGGTC GCACGTGCGC GTAAGATCCA GCGTTTCCTG TCGCAGCCGT TCCACGTCGC CGAAGTGTTC ACGGGCTCGC CGGGCAAGTA CGTGCCGCTG AAGGAAACGA TCCGCGGCTT CAAGATGATC GTCGACGGCG AGTGCGACCA CCTGCCGGAA CAGGCGTTCT ACATGGTCGG CACGATCGAC GAAGCCTTCG AGAAGGCCAA GAAGATCCAG TAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA