ButhA.17369.b

Transcription termination factor Rho (EC 3.6.4.-) (ATP-dependent helicase Rho) (BTH_I2217) (rho)

CENTER ID: ButhA.17369.b
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: diffraction
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.17369.b.A1.GE32777 full length 1 420
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
Molecular models
METHOD RESULTS
comparative modelling Robetta_81922
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_132915
PATRIC ID: fig|271848.6.peg.5037
RefSeq: YP_442738.1
UniProt: Q2SWG1
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MHLSELKSLH VSELIEMANG LEIENANRLR KQELMFAILK KRAKTGETIF GDGTLEVLPD GFGFLRSPEM SYLASTDDIY ISPSQIRRFN LHTGDTIEGE VRTPKDGERY FALVKVDKVN GQPPEASKHK IMFENLTPLH PNKPLWLERE MRGEENVTGR IIDMIAPIGK GQRGLLVASP KSGKTVMLQH IAHAIKQNHP DVILFVLLID ERPEEVTEMQ RSVAGEVIAS TFDEPATRHV QVAEMVIEKA KRLVEMKHDV VILLDSITRL ARAYNTVIPA SGKVLTGGVD ANALQRPKRF FGAARNIEEG GSLTIIGTAL IETGSRMDDV IYEEFKGTGN MEVHLERRLA EKRVYPSINL NKSGTRREEM LIKPEILQKI WVLRKFIHDM DEVEAMEFLL DKIRQTKNNA EFFDLMRRGG
NT Sequence
atgcatttat ccgagctcaa gtctctgcac gtctccgaat tgatcgaaat ggccaacggc ctggagatcg aaaacgcgaa ccgcctgcgc aagcaggagt tgatgttcgc cattctcaaa aagcgcgcca agacgggaga aacgatcttc ggcgacggca cgctcgaagt gctgcccgac ggcttcggct tcctgcgctc gccggaaatg tcgtacctcg cgagcacgga cgacatctac atcagcccgt cgcagatccg ccgcttcaac ctgcacaccg gcgacacgat cgaaggcgaa gtccgcacgc cgaaggacgg cgagcgctac ttcgcgctcg tgaaggtcga caaagtcaac gggcagccgc ccgaggcctc gaaacacaag atcatgttcg agaacctcac gccgctgcac ccgaacaagc cgctgtggct cgagcgcgaa atgcgcggtg aagagaacgt gacgggccgc atcatcgaca tgatcgcgcc gatcggcaag ggccagcgcg gcctgctcgt cgcgtcgccg aagtccggca agaccgtgat gcttcagcac atcgcgcacg cgatcaagca gaatcacccg gacgtgatcc tgttcgtgct gctgatcgac gaacgccccg aagaagtgac cgaaatgcag cgctcggtcg cgggcgaagt gatcgcgtcg acgttcgacg aaccggccac gcgccacgtg caggtcgccg aaatggtgat cgagaaggcc aagcgcctcg tcgagatgaa gcacgacgtc gtgatcctgc tcgattcgat cacgcgtctc gcacgcgcgt acaacaccgt gattccggca tcgggcaagg tgctcacggg cggcgtcgac gcgaacgcgc tgcagcgtcc gaagcgcttc ttcggcgcgg cgcgcaacat cgaggaaggc ggctcgctca cgatcatcgg caccgcgctg atcgaaaccg gcagccgcat ggacgacgtg atctacgaag agttcaaggg caccggcaac atggaagtgc acctcgagcg ccgcctcgcg gaaaagcgcg tctatccgtc gatcaacctg aacaagtcgg gcacgcgccg cgaggaaatg ctgatcaagc ccgagatcct tcagaagatc tgggtgctgc gcaagttcat ccacgacatg gacgaagtcg aggcgatgga attcctgctc gacaagatcc gccagacgaa gaacaacgcc gagttcttcg atctgatgcg ccgcggcggc tga
Details for ButhA.17369.b.A1.GE32777
HARVESTED ON: 6/20/2011
SEQUENCED ON: 1/10/2017
EXPECTED MW: 49kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MGTLEAQTQG PGSMHLSELK SLHVSELIEM ANGLEIENAN RLRKQELMFA ILKKRAKTGE TIFGDGTLEV LPDGFGFLRS PEMSYLASTD DIYISPSQIR RFNLHTGDTI EGEVRTPKDG ERYFALVKVD KVNGQPPEAS KHKIMFENLT PLHPNKPLWL EREMRGEENV TGRIIDMIAP IGKGQRGLLV ASPKSGKTVM LQHIAHAIKQ NHPDVILFVL LIDERPEEVT EMQRSVAGEV IASTFDEPAT RHVQVAEMVI EKAKRLVEMK HDVVILLDSI TRLARAYNTV IPASGKVLTG GVDANALQRP KRFFGAARNI EEGGSLTIIG TALIETGSRM DDVIYEEFKG TGNMEVHLER RLAEKRVYPS INLNKSGTRR EEMLIKPEIL QKIWVLRKFI HDMDEVEAME FLLDKIRQTK NNAEFFDLMR RGG
Validated NT Sequence
ttattagccg ccgcggcgca tcagatcgaa gaactcggcg ttgttcttcg tctggcggat cttgtcgagc aggaattcca tcgcctcgac ttcgtccatg tcgtggatga acttgcgcag cacccagatc ttctgaagga tctcgggctt gatcagcatt tcctcgcggc gcgtgcccga cttgttcagg ttgatcgacg gatagacgcg cttttccgcg aggcggcgct cgaggtgcac ttccatgttg ccggtgccct tgaactcttc gtagatcacg tcgtccatgc ggctgccggt ttcgatcagc gcggtgccga tgatcgtgag cgagccgcct tcctcgatgt tgcgcgccgc gccgaagaag cgcttcggac gctgcagcgc gttcgcgtcg acgccgcccg tgagcacctt gcccgatgcc ggaatcacgg tgttgtacgc gcgtgcgaga cgcgtgatcg aatcgagcag gatcacgacg tcgtgcttca tctcgacgag gcgcttggcc ttctcgatca ccatttcggc gacctgcacg tggcgcgtgg ccggttcgtc gaacgtcgac gcgatcactt cgcccgcgac cgagcgctgc atttcggtca cttcttcggg gcgttcgtcg atcagcagca cgaacaggat cacgtccggg tgattctgct tgatcgcgtg cgcgatgtgc tgaagcatca cggtcttgcc ggacttcggc gacgcgacga gcaggccgcg ctggcccttg ccgatcggcg cgatcatgtc gatgatgcgg cccgtcacgt tctcttcacc gcgcatttcg cgctcgagcc acagcggctt gttcgggtgc agcggcgtga ggttctcgaa catgatcttg tgtttcgagg cctcgggcgg ctgcccgttg actttgtcga ccttcacgag cgcgaagtag cgctcgccgt ccttcggcgt gcggacttcg ccttcgatcg tgtcgccggt gtgcaggttg aagcggcgga tctgcgacgg gctgatgtag atgtcgtccg tgctcgcgag gtacgacatt tccggcgagc gcaggaagcc gaagccgtcg ggcagcactt cgagcgtgcc gtcgccgaag atcgtttctc ccgtcttggc gcgctttttg agaatggcga acatcaactc ctgcttgcgc aggcggttcg cgttttcgat ctccaggccg ttggccattt cgatcaattc ggagacgtgc agagacttga gctcggataa atgcatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagcccnt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMHLSELKSL HVSELIEMAN GLEIENANRL RKQELMFAIL KKRAKTGETI FGDGTLEVLP DGFGFLRSPE MSYLASTDDI YISPSQIRRF NLHTGDTIEG EVRTPKDGER YFALVKVDKV NGQPPEASKH KIMFENLTPL HPNKPLWLER EMRGEENVTG RIIDMIAPIG KGQRGLLVAS PKSGKTVMLQ HIAHAIKQNH PDVILFVLLI DERPEEVTEM QRSVAGEVIA STFDEPATRH VQVAEMVIEK AKRLVEMKHD VVILLDSITR LARAYNTVIP ASGKVLTGGV DANALQRPKR FFGAARNIEE GGSLTIIGTA LIETGSRMDD VIYEEFKGTG NMEVHLERRL AEKRVYPSIN LNKSGTRREE MLIKPEILQK IWVLRKFIHD MDEVEAMEFL LDKIRQTKNN AEFFDLMRRG G
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcatttatcc gagctcaagt ctctgcacgt ctccgaattg atcgaaatgg ccaacggcct ggagatcgaa aacgcgaacc gcctgcgcaa gcaggagttg atgttcgcca ttctcaaaaa gcgcgccaag acgggagaaa cgatcttcgg cgacggcacg ctcgaagtgc tgcccgacgg cttcggcttc ctgcgctcgc cggaaatgtc gtacctcgcg agcacggacg acatctacat cagcccgtcg cagatccgcc gcttcaacct gcacaccggc gacacgatcg aaggcgaagt ccgcacgccg aaggacggcg agcgctactt cgcgctcgtg aaggtcgaca aagtcaacgg gcagccgccc gaggcctcga aacacaagat catgttcgag aacctcacgc cgctgcaccc gaacaagccg ctgtggctcg agcgcgaaat gcgcggtgaa gagaacgtga cgggccgcat catcgacatg atcgcgccga tcggcaaggg ccagcgcggc ctgctcgtcg cgtcgccgaa gtccggcaag accgtgatgc ttcagcacat cgcgcacgcg atcaagcaga atcacccgga cgtgatcctg ttcgtgctgc tgatcgacga acgccccgaa gaagtgaccg aaatgcagcg ctcggtcgcg ggcgaagtga tcgcgtcgac gttcgacgaa ccggccacgc gccacgtgca ggtcgccgaa atggtgatcg agaaggccaa gcgcctcgtc gagatgaagc acgacgtcgt gatcctgctc gattcgatca cgcgtctcgc acgcgcgtac aacaccgtga ttccggcatc gggcaaggtg ctcacgggcg gcgtcgacgc gaacgcgctg cagcgtccga agcgcttctt cggcgcggcg cgcaacatcg aggaaggcgg ctcgctcacg atcatcggca ccgcgctgat cgaaaccggc agccgcatgg acgacgtgat ctacgaagag ttcaagggca ccggcaacat ggaagtgcac ctcgagcgcc gcctcgcgga aaagcgcgtc tatccgtcga tcaacctgaa caagtcgggc acgcgccgcg aggaaatgct gatcaagccc gagatccttc agaagatctg ggtgctgcgc aagttcatcc acgacatgga cgaagtcgag gcgatggaat tcctgctcga caagatccgc cagacgaaga acaacgccga gttcttcgat ctgatgcgcc gcggcggcta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a