ButhA.17369.a

ATP synthase subunit alpha 1 (EC 3.6.3.14) (ATP synthase F1 sector subunit alpha 1) (F-ATPase subunit alpha 1) (BTH_I3310) (atpA1)

CENTER ID: ButhA.17369.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.17369.a.A1.GE32776 full length 1 513
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.17369.a.A1.PD00491 full length 1 513
ButhA.17369.a.A1.PW35957 full length 1 513

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.6197
RefSeq: YP_443802.1
UniProt: Q2STE7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQLNPSEISE LIKSRIQGLE ASADVRNQGT VISVTDGIVR IHGLSDVMQG EMLEFPGNTF GLALNLERDS VGAVILGEYE HISEGDIVKT TGRILEVPVG PELVGRVVDA LGNPIDGKGP VNAKLTDAIE KIAPGVIWRK SVSQPVQTGL KSIDSMVPIG RGQRELIIGD RQCGKTAVAI DTIINQKGKD LICIYVAIGQ KASSIMNVVR KLEETGALEY TIVVAASASE SAAMQYLAPY AGCTMGEYFR DRGQDALIIY DDLTKQAWAY RQISLLLRRP PGREAYPGDV FYLHSRLLER AARVSEEYVE KFTNGEVKGK SGSLTALPVI ETQAGDVTAF VPTNVISITD GQIFLETDLF NAGIRPAINA GVSVSRVGGA AQTKVVKKLS GGIRTDLAQY RELAAFAQFA SDLDEATRKQ LERGRRVTEL LKQPQYQPLQ VWELAVSLFS ANNGYLDDLD VKDVLPFEKG LREYLKTSHA DLIKRIEDTK DLSKDDESAL HAAIKDFKKS GAY
NT Sequence
atgcaactca atccctctga gatcagcgag ctgatcaaga gccggatcca gggccttgaa gcgagcgcag acgttcgcaa ccagggcacc gtgatctccg tgaccgacgg tatcgtgcgc attcacggcc tgtcggacgt gatgcagggc gaaatgctcg aatttccggg caacacgttc ggcctcgcgc tgaacctcga gcgcgactcg gtcggcgcgg tgattctcgg cgaatacgag cacatctcgg aaggcgacat cgtcaagacg acgggccgca ttctcgaagt gccggtcggc ccggaactcg tcggtcgcgt ggtcgacgcg ctcggtaacc cgatcgacgg caagggcccg gtcaacgcga agctgaccga cgcgatcgaa aagatcgccc cgggcgtgat ctggcgtaag tcggtgtcgc agccggtgca gacgggcctg aagtcgatcg actcgatggt gccgatcggc cgcggccagc gcgagctgat catcggcgac cgtcagtgcg gcaagaccgc ggtggcgatc gacacgatca tcaaccagaa gggcaaggac ctcatctgta tctacgtcgc gatcggccag aaggcttcgt cgatcatgaa cgtggtgcgc aagctcgagg aaaccggcgc gctcgaatac acgatcgtcg tcgccgcgtc ggcgtcggaa tcggccgcga tgcaatacct cgcgccgtac gccggctgca cgatgggcga atacttccgc gatcgcggcc aggacgccct catcatttac gacgatttga ccaagcaagc ttgggcgtat cgtcagatct cgctgctgct gcgccgtccg ccgggccgcg aagcgtaccc gggcgacgtg ttctatctgc actcgcgtct gctcgagcgc gcggcgcgcg tgtcggaaga gtatgtcgag aagttcacga acggtgaagt gaagggcaag agcggctcgc tgacggcgct gcccgtcatc gaaacgcagg cgggcgacgt gaccgcgttc gttccgacga acgtgatctc gatcaccgac ggccagatct tcctggaaac cgacctgttc aacgccggca tccgccccgc gatcaacgcg ggcgtgtcgg tgtcgcgagt cggcggcgcc gcgcagacga aggtcgtgaa gaagctgtcg ggcggtatcc gtaccgacct cgcgcagtac cgcgaactgg ccgcgttcgc gcagttcgca tcggatctcg acgaagcgac ccgcaagcag ctcgagcgcg gccgccgcgt gacggaactg ctcaagcagc cgcaatacca gccgctgcag gtgtgggagc tggccgtgtc gctgttctcg gcgaacaacg gctatctcga cgacctcgac gtcaaggacg tcctgccgtt cgagaagggc ctgcgcgagt atctgaagac gagccacgct gacctcatca agcgcatcga agacacgaaa gacttgtcga aggacgacga gagcgcgctg cacgccgcga tcaaggactt caagaagtcg ggtgcctatt ga
Details for ButhA.17369.a.A1.GE32776
HARVESTED ON: 6/20/2011
SEQUENCED ON: 7/14/2011
EXPECTED MW: 58kDa
OBSERVED MW: 60kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMQLNPSEIS ELIKSRIQGL EASADVRNQG TVISVTDGIV RIHGLSDVMQ GEMLEFPGNT FGLALNLERD SVGAVILGEY EHISEGDIVK TTGRILEVPV GPELVGRVVD ALGNPIDGKG PVNAKLTDAI EKIAPGVIWR KSVSQPVQTG LKSIDSMVPI GRGQRELIIG DRQCGKTAVA IDTIINQXGK DLICIYVAIG QKASSIMNVV RKLEETGALE YTIVVAASAS ESAAMQYLAP YAGCTMGEYF RDRGQDALII YDDLTKQAWA YRQISLLLRR PPGREAYPGD VFYLHSRLLE RAARVSEEYV EKFTNGEVKG KSGSLTALPV IETQAGDVTA FVPTNVISIT DGQIFLETDL FNAGIRPAIN AGVSVSRVGG AAQTKVVKKL SGGIRTDLAQ YRELAAFAQF ASDLDEATRK QLERGRRVTE LLKQPQYQPL QVWELAVSLF SANNGYLDDL DVKDVLPFEK GLREYLKTSH ADLIKRIEDT KDLSKDDESA LHAAIKDFKK SGAY
Validated NT Sequence
ttattaatag gcacccgact tcttgaagtc cttgatcgcg gcgtgcagcg cgctctcgtc gtccttcgac aagtctttcg tgtcttcgat gcgcttgatg aggtcagcgt ggctcgtctt cagatactcg cgcaggccct tctcgaacgg caggacgtcc ttgacgtcga ggtcgtcgag atagccgttg ttcgccgaga acagcgacac ggccagctcc cacacctgca gcggctggta ttgcggctgc ttgagcagtt ccgtcacgcg gcggccgcgc tcgagctgct tgcgggtcgc ttcgtcgaga tccgatgcga actgcgcgaa cgcggccagt tcgcggtact gcgcgaggtc ggtacggata ccgcccgaca gcttcttcac gaccttcgtc tgcgcggcgc cgccgactcg cgacaccgac acgcccgcgt tgatcgcggg gcggatgccg gcgttgaaca ggtcggtttc caggaagatc tggccgtcgg tgatcgagat cacgttcgtc ggaacgaacg cggtcacgtc gcccgcctgc gtttcgatga cgggcagcgc cgtcagcgag ccgctcttgc ccttcacttc accgttcgtg aacttctcga catactcttc cgacacgcgc gccgcgcgct cgagcagacg cgagtgcaga tagaacacgt cgcccgggta cgcttcgcgg cccggcggac ggcgcagcag cagcgagatc tgacgatacg cccaagcttg cttggtcaaa tcgtcgtaaa tgatgagggc gtcctggccg cgatcgcgga agtattcgcc catcgtgcag ccggcgtacg gcgcgaggta ttgcatcgcg gccgattccg acgccgacgc ggcgacgacg atcgtgtatt cgagcgcgcc ggtttcctcg agcttgcgca ccacgttcat gatcgacgaa gccttctggc cgatcgcgac gtagatacag atgaggtcct tgccnttctg gttgatgatc gtgtcgatcg ccaccgcggt cttgccgcac tgacggtcgc cgatgatcag ctcgcgctgg ccgcggccga tcggcaccat cgagtcgatc gacttcaggc ccgtctgcac cggctgcgac accgacttac gccagatcac gcccggggcg atcttttcga tcgcgtcggt cagcttcgcg ttgaccgggc ccttgccgtc gatcgggtta ccgagcgcgt cgaccacgcg accgacgagt tccgggccga ccggcacttc gagaatgcgg cccgtcgtct tgacgatgtc gccttccgag atgtgctcgt attcgccgag aatcaccgcg ccgaccgagt cgcgctcgag gttcagcgcg aggccgaacg tgttgcccgg aaattcgagc atttcgccct gcatcacgtc cgacaggccg tgaatgcgca cgataccgtc ggtcacggag atcacggtgc cctggttgcg aacgtctgcg ctcgcttcaa ggccctggat ccggctcttg atcagctcgc tgatctcaga gggattgagt tgcatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQLNPSEIS ELIKSRIQGL EASADVRNQG TVISVTDGIV RIHGLSDVMQ GEMLEFPGNT FGLALNLERD SVGAVILGEY EHISEGDIVK TTGRILEVPV GPELVGRVVD ALGNPIDGKG PVNAKLTDAI EKIAPGVIWR KSVSQPVQTG LKSIDSMVPI GRGQRELIIG DRQCGKTAVA IDTIINQKGK DLICIYVAIG QKASSIMNVV RKLEETGALE YTIVVAASAS ESAAMQYLAP YAGCTMGEYF RDRGQDALII YDDLTKQAWA YRQISLLLRR PPGREAYPGD VFYLHSRLLE RAARVSEEYV EKFTNGEVKG KSGSLTALPV IETQAGDVTA FVPTNVISIT DGQIFLETDL FNAGIRPAIN AGVSVSRVGG AAQTKVVKKL SGGIRTDLAQ YRELAAFAQF ASDLDEATRK QLERGRRVTE LLKQPQYQPL QVWELAVSLF SANNGYLDDL DVKDVLPFEK GLREYLKTSH ADLIKRIEDT KDLSKDDESA LHAAIKDFKK SGAY
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcaactcaat ccctctgaga tcagcgagct gatcaagagc cggatccagg gccttgaagc gagcgcagac gttcgcaacc agggcaccgt gatctccgtg accgacggta tcgtgcgcat tcacggcctg tcggacgtga tgcagggcga aatgctcgaa tttccgggca acacgttcgg cctcgcgctg aacctcgagc gcgactcggt cggcgcggtg attctcggcg aatacgagca catctcggaa ggcgacatcg tcaagacgac gggccgcatt ctcgaagtgc cggtcggccc ggaactcgtc ggtcgcgtgg tcgacgcgct cggtaacccg atcgacggca agggcccggt caacgcgaag ctgaccgacg cgatcgaaaa gatcgccccg ggcgtgatct ggcgtaagtc ggtgtcgcag ccggtgcaga cgggcctgaa gtcgatcgac tcgatggtgc cgatcggccg cggccagcgc gagctgatca tcggcgaccg tcagtgcggc aagaccgcgg tggcgatcga cacgatcatc aaccagaagg gcaaggacct catctgtatc tacgtcgcga tcggccagaa ggcttcgtcg atcatgaacg tggtgcgcaa gctcgaggaa accggcgcgc tcgaatacac gatcgtcgtc gccgcgtcgg cgtcggaatc ggccgcgatg caatacctcg cgccgtacgc cggctgcacg atgggcgaat acttccgcga tcgcggccag gacgccctca tcatttacga cgatttgacc aagcaagctt gggcgtatcg tcagatctcg ctgctgctgc gccgtccgcc gggccgcgaa gcgtacccgg gcgacgtgtt ctatctgcac tcgcgtctgc tcgagcgcgc ggcgcgcgtg tcggaagagt atgtcgagaa gttcacgaac ggtgaagtga agggcaagag cggctcgctg acggcgctgc ccgtcatcga aacgcaggcg ggcgacgtga ccgcgttcgt tccgacgaac gtgatctcga tcaccgacgg ccagatcttc ctggaaaccg acctgttcaa cgccggcatc cgccccgcga tcaacgcggg cgtgtcggtg tcgcgagtcg gcggcgccgc gcagacgaag gtcgtgaaga agctgtcggg cggtatccgt accgacctcg cgcagtaccg cgaactggcc gcgttcgcgc agttcgcatc ggatctcgac gaagcgaccc gcaagcagct cgagcgcggc cgccgcgtga cggaactgct caagcagccg caataccagc cgctgcaggt gtgggagctg gccgtgtcgc tgttctcggc gaacaacggc tatctcgacg acctcgacgt caaggacgtc ctgccgttcg agaagggcct gcgcgagtat ctgaagacga gccacgctga cctcatcaag cgcatcgaag acacgaaaga cttgtcgaag gacgacgaga gcgcgctgca cgccgcgatc aaggacttca agaagtcggg tgcctattaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for ButhA.17369.a.A1.PD00491
PURIFICATION DATe: 4/5/2017
CONCENTRATION: 15.25mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25mM tris pH 8.0, 200mM NaCl, 1% glycerol, 1mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 100µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMQLNPSEIS ELIKSRIQGL EASADVRNQG TVISVTDGIV RIHGLSDVMQ GEMLEFPGNT FGLALNLERD SVGAVILGEY EHISEGDIVK TTGRILEVPV GPELVGRVVD ALGNPIDGKG PVNAKLTDAI EKIAPGVIWR KSVSQPVQTG LKSIDSMVPI GRGQRELIIG DRQCGKTAVA IDTIINQXGK DLICIYVAIG QKASSIMNVV RKLEETGALE YTIVVAASAS ESAAMQYLAP YAGCTMGEYF RDRGQDALII YDDLTKQAWA YRQISLLLRR PPGREAYPGD VFYLHSRLLE RAARVSEEYV EKFTNGEVKG KSGSLTALPV IETQAGDVTA FVPTNVISIT DGQIFLETDL FNAGIRPAIN AGVSVSRVGG AAQTKVVKKL SGGIRTDLAQ YRELAAFAQF ASDLDEATRK QLERGRRVTE LLKQPQYQPL QVWELAVSLF SANNGYLDDL DVKDVLPFEK GLREYLKTSH ADLIKRIEDT KDLSKDDESA LHAAIKDFKK SGAY
Validated NT Sequence
ttattaatag gcacccgact tcttgaagtc cttgatcgcg gcgtgcagcg cgctctcgtc gtccttcgac aagtctttcg tgtcttcgat gcgcttgatg aggtcagcgt ggctcgtctt cagatactcg cgcaggccct tctcgaacgg caggacgtcc ttgacgtcga ggtcgtcgag atagccgttg ttcgccgaga acagcgacac ggccagctcc cacacctgca gcggctggta ttgcggctgc ttgagcagtt ccgtcacgcg gcggccgcgc tcgagctgct tgcgggtcgc ttcgtcgaga tccgatgcga actgcgcgaa cgcggccagt tcgcggtact gcgcgaggtc ggtacggata ccgcccgaca gcttcttcac gaccttcgtc tgcgcggcgc cgccgactcg cgacaccgac acgcccgcgt tgatcgcggg gcggatgccg gcgttgaaca ggtcggtttc caggaagatc tggccgtcgg tgatcgagat cacgttcgtc ggaacgaacg cggtcacgtc gcccgcctgc gtttcgatga cgggcagcgc cgtcagcgag ccgctcttgc ccttcacttc accgttcgtg aacttctcga catactcttc cgacacgcgc gccgcgcgct cgagcagacg cgagtgcaga tagaacacgt cgcccgggta cgcttcgcgg cccggcggac ggcgcagcag cagcgagatc tgacgatacg cccaagcttg cttggtcaaa tcgtcgtaaa tgatgagggc gtcctggccg cgatcgcgga agtattcgcc catcgtgcag ccggcgtacg gcgcgaggta ttgcatcgcg gccgattccg acgccgacgc ggcgacgacg atcgtgtatt cgagcgcgcc ggtttcctcg agcttgcgca ccacgttcat gatcgacgaa gccttctggc cgatcgcgac gtagatacag atgaggtcct tgccnttctg gttgatgatc gtgtcgatcg ccaccgcggt cttgccgcac tgacggtcgc cgatgatcag ctcgcgctgg ccgcggccga tcggcaccat cgagtcgatc gacttcaggc ccgtctgcac cggctgcgac accgacttac gccagatcac gcccggggcg atcttttcga tcgcgtcggt cagcttcgcg ttgaccgggc ccttgccgtc gatcgggtta ccgagcgcgt cgaccacgcg accgacgagt tccgggccga ccggcacttc gagaatgcgg cccgtcgtct tgacgatgtc gccttccgag atgtgctcgt attcgccgag aatcaccgcg ccgaccgagt cgcgctcgag gttcagcgcg aggccgaacg tgttgcccgg aaattcgagc atttcgccct gcatcacgtc cgacaggccg tgaatgcgca cgataccgtc ggtcacggag atcacggtgc cctggttgcg aacgtctgcg ctcgcttcaa ggccctggat ccggctcttg atcagctcgc tgatctcaga gggattgagt tgcatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQLNPSEIS ELIKSRIQGL EASADVRNQG TVISVTDGIV RIHGLSDVMQ GEMLEFPGNT FGLALNLERD SVGAVILGEY EHISEGDIVK TTGRILEVPV GPELVGRVVD ALGNPIDGKG PVNAKLTDAI EKIAPGVIWR KSVSQPVQTG LKSIDSMVPI GRGQRELIIG DRQCGKTAVA IDTIINQKGK DLICIYVAIG QKASSIMNVV RKLEETGALE YTIVVAASAS ESAAMQYLAP YAGCTMGEYF RDRGQDALII YDDLTKQAWA YRQISLLLRR PPGREAYPGD VFYLHSRLLE RAARVSEEYV EKFTNGEVKG KSGSLTALPV IETQAGDVTA FVPTNVISIT DGQIFLETDL FNAGIRPAIN AGVSVSRVGG AAQTKVVKKL SGGIRTDLAQ YRELAAFAQF ASDLDEATRK QLERGRRVTE LLKQPQYQPL QVWELAVSLF SANNGYLDDL DVKDVLPFEK GLREYLKTSH ADLIKRIEDT KDLSKDDESA LHAAIKDFKK SGAY
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCAACTCAAT CCCTCTGAGA TCAGCGAGCT GATCAAGAGC CGGATCCAGG GCCTTGAAGC GAGCGCAGAC GTTCGCAACC AGGGCACCGT GATCTCCGTG ACCGACGGTA TCGTGCGCAT TCACGGCCTG TCGGACGTGA TGCAGGGCGA AATGCTCGAA TTTCCGGGCA ACACGTTCGG CCTCGCGCTG AACCTCGAGC GCGACTCGGT CGGCGCGGTG ATTCTCGGCG AATACGAGCA CATCTCGGAA GGCGACATCG TCAAGACGAC GGGCCGCATT CTCGAAGTGC CGGTCGGCCC GGAACTCGTC GGTCGCGTGG TCGACGCGCT CGGTAACCCG ATCGACGGCA AGGGCCCGGT CAACGCGAAG CTGACCGACG CGATCGAAAA GATCGCCCCG GGCGTGATCT GGCGTAAGTC GGTGTCGCAG CCGGTGCAGA CGGGCCTGAA GTCGATCGAC TCGATGGTGC CGATCGGCCG CGGCCAGCGC GAGCTGATCA TCGGCGACCG TCAGTGCGGC AAGACCGCGG TGGCGATCGA CACGATCATC AACCAGAAGG GCAAGGACCT CATCTGTATC TACGTCGCGA TCGGCCAGAA GGCTTCGTCG ATCATGAACG TGGTGCGCAA GCTCGAGGAA ACCGGCGCGC TCGAATACAC GATCGTCGTC GCCGCGTCGG CGTCGGAATC GGCCGCGATG CAATACCTCG CGCCGTACGC CGGCTGCACG ATGGGCGAAT ACTTCCGCGA TCGCGGCCAG GACGCCCTCA TCATTTACGA CGATTTGACC AAGCAAGCTT GGGCGTATCG TCAGATCTCG CTGCTGCTGC GCCGTCCGCC GGGCCGCGAA GCGTACCCGG GCGACGTGTT CTATCTGCAC TCGCGTCTGC TCGAGCGCGC GGCGCGCGTG TCGGAAGAGT ATGTCGAGAA GTTCACGAAC GGTGAAGTGA AGGGCAAGAG CGGCTCGCTG ACGGCGCTGC CCGTCATCGA AACGCAGGCG GGCGACGTGA CCGCGTTCGT TCCGACGAAC GTGATCTCGA TCACCGACGG CCAGATCTTC CTGGAAACCG ACCTGTTCAA CGCCGGCATC CGCCCCGCGA TCAACGCGGG CGTGTCGGTG TCGCGAGTCG GCGGCGCCGC GCAGACGAAG GTCGTGAAGA AGCTGTCGGG CGGTATCCGT ACCGACCTCG CGCAGTACCG CGAACTGGCC GCGTTCGCGC AGTTCGCATC GGATCTCGAC GAAGCGACCC GCAAGCAGCT CGAGCGCGGC CGCCGCGTGA CGGAACTGCT CAAGCAGCCG CAATACCAGC CGCTGCAGGT GTGGGAGCTG GCCGTGTCGC TGTTCTCGGC GAACAACGGC TATCTCGACG ACCTCGACGT CAAGGACGTC CTGCCGTTCG AGAAGGGCCT GCGCGAGTAT CTGAAGACGA GCCACGCTGA CCTCATCAAG CGCATCGAAG ACACGAAAGA CTTGTCGAAG GACGACGAGA GCGCGCTGCA CGCCGCGATC AAGGACTTCA AGAAGTCGGG TGCCTATTAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA
Details for ButhA.17369.a.A1.PW35957
PURIFICATION DATe: 10/14/2011
CONCENTRATION: 27.51mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 100µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMQLNPSEIS ELIKSRIQGL EASADVRNQG TVISVTDGIV RIHGLSDVMQ GEMLEFPGNT FGLALNLERD SVGAVILGEY EHISEGDIVK TTGRILEVPV GPELVGRVVD ALGNPIDGKG PVNAKLTDAI EKIAPGVIWR KSVSQPVQTG LKSIDSMVPI GRGQRELIIG DRQCGKTAVA IDTIINQXGK DLICIYVAIG QKASSIMNVV RKLEETGALE YTIVVAASAS ESAAMQYLAP YAGCTMGEYF RDRGQDALII YDDLTKQAWA YRQISLLLRR PPGREAYPGD VFYLHSRLLE RAARVSEEYV EKFTNGEVKG KSGSLTALPV IETQAGDVTA FVPTNVISIT DGQIFLETDL FNAGIRPAIN AGVSVSRVGG AAQTKVVKKL SGGIRTDLAQ YRELAAFAQF ASDLDEATRK QLERGRRVTE LLKQPQYQPL QVWELAVSLF SANNGYLDDL DVKDVLPFEK GLREYLKTSH ADLIKRIEDT KDLSKDDESA LHAAIKDFKK SGAY
Validated NT Sequence
ttattaatag gcacccgact tcttgaagtc cttgatcgcg gcgtgcagcg cgctctcgtc gtccttcgac aagtctttcg tgtcttcgat gcgcttgatg aggtcagcgt ggctcgtctt cagatactcg cgcaggccct tctcgaacgg caggacgtcc ttgacgtcga ggtcgtcgag atagccgttg ttcgccgaga acagcgacac ggccagctcc cacacctgca gcggctggta ttgcggctgc ttgagcagtt ccgtcacgcg gcggccgcgc tcgagctgct tgcgggtcgc ttcgtcgaga tccgatgcga actgcgcgaa cgcggccagt tcgcggtact gcgcgaggtc ggtacggata ccgcccgaca gcttcttcac gaccttcgtc tgcgcggcgc cgccgactcg cgacaccgac acgcccgcgt tgatcgcggg gcggatgccg gcgttgaaca ggtcggtttc caggaagatc tggccgtcgg tgatcgagat cacgttcgtc ggaacgaacg cggtcacgtc gcccgcctgc gtttcgatga cgggcagcgc cgtcagcgag ccgctcttgc ccttcacttc accgttcgtg aacttctcga catactcttc cgacacgcgc gccgcgcgct cgagcagacg cgagtgcaga tagaacacgt cgcccgggta cgcttcgcgg cccggcggac ggcgcagcag cagcgagatc tgacgatacg cccaagcttg cttggtcaaa tcgtcgtaaa tgatgagggc gtcctggccg cgatcgcgga agtattcgcc catcgtgcag ccggcgtacg gcgcgaggta ttgcatcgcg gccgattccg acgccgacgc ggcgacgacg atcgtgtatt cgagcgcgcc ggtttcctcg agcttgcgca ccacgttcat gatcgacgaa gccttctggc cgatcgcgac gtagatacag atgaggtcct tgccnttctg gttgatgatc gtgtcgatcg ccaccgcggt cttgccgcac tgacggtcgc cgatgatcag ctcgcgctgg ccgcggccga tcggcaccat cgagtcgatc gacttcaggc ccgtctgcac cggctgcgac accgacttac gccagatcac gcccggggcg atcttttcga tcgcgtcggt cagcttcgcg ttgaccgggc ccttgccgtc gatcgggtta ccgagcgcgt cgaccacgcg accgacgagt tccgggccga ccggcacttc gagaatgcgg cccgtcgtct tgacgatgtc gccttccgag atgtgctcgt attcgccgag aatcaccgcg ccgaccgagt cgcgctcgag gttcagcgcg aggccgaacg tgttgcccgg aaattcgagc atttcgccct gcatcacgtc cgacaggccg tgaatgcgca cgataccgtc ggtcacggag atcacggtgc cctggttgcg aacgtctgcg ctcgcttcaa ggccctggat ccggctcttg atcagctcgc tgatctcaga gggattgagt tgcatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQLNPSEIS ELIKSRIQGL EASADVRNQG TVISVTDGIV RIHGLSDVMQ GEMLEFPGNT FGLALNLERD SVGAVILGEY EHISEGDIVK TTGRILEVPV GPELVGRVVD ALGNPIDGKG PVNAKLTDAI EKIAPGVIWR KSVSQPVQTG LKSIDSMVPI GRGQRELIIG DRQCGKTAVA IDTIINQKGK DLICIYVAIG QKASSIMNVV RKLEETGALE YTIVVAASAS ESAAMQYLAP YAGCTMGEYF RDRGQDALII YDDLTKQAWA YRQISLLLRR PPGREAYPGD VFYLHSRLLE RAARVSEEYV EKFTNGEVKG KSGSLTALPV IETQAGDVTA FVPTNVISIT DGQIFLETDL FNAGIRPAIN AGVSVSRVGG AAQTKVVKKL SGGIRTDLAQ YRELAAFAQF ASDLDEATRK QLERGRRVTE LLKQPQYQPL QVWELAVSLF SANNGYLDDL DVKDVLPFEK GLREYLKTSH ADLIKRIEDT KDLSKDDESA LHAAIKDFKK SGAY
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCAACTCAAT CCCTCTGAGA TCAGCGAGCT GATCAAGAGC CGGATCCAGG GCCTTGAAGC GAGCGCAGAC GTTCGCAACC AGGGCACCGT GATCTCCGTG ACCGACGGTA TCGTGCGCAT TCACGGCCTG TCGGACGTGA TGCAGGGCGA AATGCTCGAA TTTCCGGGCA ACACGTTCGG CCTCGCGCTG AACCTCGAGC GCGACTCGGT CGGCGCGGTG ATTCTCGGCG AATACGAGCA CATCTCGGAA GGCGACATCG TCAAGACGAC GGGCCGCATT CTCGAAGTGC CGGTCGGCCC GGAACTCGTC GGTCGCGTGG TCGACGCGCT CGGTAACCCG ATCGACGGCA AGGGCCCGGT CAACGCGAAG CTGACCGACG CGATCGAAAA GATCGCCCCG GGCGTGATCT GGCGTAAGTC GGTGTCGCAG CCGGTGCAGA CGGGCCTGAA GTCGATCGAC TCGATGGTGC CGATCGGCCG CGGCCAGCGC GAGCTGATCA TCGGCGACCG TCAGTGCGGC AAGACCGCGG TGGCGATCGA CACGATCATC AACCAGAAGG GCAAGGACCT CATCTGTATC TACGTCGCGA TCGGCCAGAA GGCTTCGTCG ATCATGAACG TGGTGCGCAA GCTCGAGGAA ACCGGCGCGC TCGAATACAC GATCGTCGTC GCCGCGTCGG CGTCGGAATC GGCCGCGATG CAATACCTCG CGCCGTACGC CGGCTGCACG ATGGGCGAAT ACTTCCGCGA TCGCGGCCAG GACGCCCTCA TCATTTACGA CGATTTGACC AAGCAAGCTT GGGCGTATCG TCAGATCTCG CTGCTGCTGC GCCGTCCGCC GGGCCGCGAA GCGTACCCGG GCGACGTGTT CTATCTGCAC TCGCGTCTGC TCGAGCGCGC GGCGCGCGTG TCGGAAGAGT ATGTCGAGAA GTTCACGAAC GGTGAAGTGA AGGGCAAGAG CGGCTCGCTG ACGGCGCTGC CCGTCATCGA AACGCAGGCG GGCGACGTGA CCGCGTTCGT TCCGACGAAC GTGATCTCGA TCACCGACGG CCAGATCTTC CTGGAAACCG ACCTGTTCAA CGCCGGCATC CGCCCCGCGA TCAACGCGGG CGTGTCGGTG TCGCGAGTCG GCGGCGCCGC GCAGACGAAG GTCGTGAAGA AGCTGTCGGG CGGTATCCGT ACCGACCTCG CGCAGTACCG CGAACTGGCC GCGTTCGCGC AGTTCGCATC GGATCTCGAC GAAGCGACCC GCAAGCAGCT CGAGCGCGGC CGCCGCGTGA CGGAACTGCT CAAGCAGCCG CAATACCAGC CGCTGCAGGT GTGGGAGCTG GCCGTGTCGC TGTTCTCGGC GAACAACGGC TATCTCGACG ACCTCGACGT CAAGGACGTC CTGCCGTTCG AGAAGGGCCT GCGCGAGTAT CTGAAGACGA GCCACGCTGA CCTCATCAAG CGCATCGAAG ACACGAAAGA CTTGTCGAAG GACGACGAGA GCGCGCTGCA CGCCGCGATC AAGGACTTCA AGAAGTCGGG TGCCTATTAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA