ButhA.17336.a

Lipopolysaccharide heptosyltransferase I (EC 2.-.-.-) (BTH_I1490) (rfaC-1)

CENTER ID: ButhA.17336.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.17336.a.A1.GE34795 full length 1 355
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.4272
RefSeq: YP_442036.1
UniProt: Q2SYG3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPAARRDKIR PFGLCRPPPA IFLSVQKILI VRVSSLGDVV HNMPVIADIR RRHPDAQIDW LVEEGFADLV RLVDGVRNVL PFSLRRWRKR LSASQTWREI RAFRRRLAEQ RYDLVIDCQG LIKTAWVASW ARGPLVGLGN RTDGAGYEWP VRFFYDKRVP IAPRTHVVER SRQLVAAALG DPAPAPDDPI DFGLDTHGAA RALASLDLNL PVPYVVFVHA TSRADKQWPD DAWIGLGEAL VRRGASLVLP WGSDAERATS ERLAKAFGAA AIVPPKLSLP AVVGLIDGAA ATVGVDTGLV HIAAALKRPT VELYNFATAW RTGGYWSANV VNLGTAGAPP SLSQTKDALA SFGLL
NT Sequence
gtgccggccg cccggcgcga taaaatccgt cctttcggtc tgtgccggcc gccgccggcc atttttctca gcgtgcaaaa aattctgatc gtgcgcgtgt cgtcgctcgg cgacgtcgtg cataacatgc cggtgatcgc cgatattcgc cggcgccatc ccgatgcgca gatcgactgg ctcgtcgagg aaggcttcgc cgatctcgtg cggctcgtcg acggcgtgcg caacgtgctg ccgttctcgc tgcggcgctg gcgcaagcgc ctgagcgcat cgcagacgtg gcgcgagatc cgcgcattcc ggcggcgtct cgccgaacag cgctacgacc tcgtgatcga ctgccagggg ctcatcaaga ccgcgtgggt cgcgagctgg gcgcgcgggc cgctcgtcgg cctcggcaac cgcaccgacg gcgccggcta cgaatggccg gtgcgcttct tctacgacaa gcgcgtgccg atcgcgccgc gcacgcacgt cgtcgagcgc tcgcggcaac tcgtcgcggc ggcgctggga gaccccgcgc cggcgcccga cgatccgatc gacttcggcc tcgacacgca cggcgcggcg cgcgcgctcg cgtcgctcga tttgaacctg ccggtgcctt acgtcgtgtt cgtgcatgcg acgtcgcgcg ccgacaagca gtggcccgac gacgcgtgga tcggcctggg cgaggcgctc gtgcggcgcg gcgcgtcgct cgtgctgccg tggggcagcg acgccgagcg cgcgacgagc gagcggctcg cgaaggcgtt cggcgcggcg gcgatcgtgc cgccgaagct gtcgctgccc gcggtcgtcg gcttgatcga cggtgcggcc gcgacggtcg gcgtcgatac cggcctcgtc cacatcgcgg cggcgctcaa gcgcccgacc gtcgaactgt acaatttcgc gacagcctgg cgcacgggcg gctactggtc ggccaacgtc gtcaacctcg gcaccgccgg cgcgccgcca tccctttcgc agacgaagga cgccctcgcg tcgttcggcc tcttgtaa
Details for ButhA.17336.a.A1.GE34795
HARVESTED ON: 3/13/2012
SEQUENCED ON: 3/12/2012
EXPECTED MW: 41kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 72
PERCENT COVERAGE: 13
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVPAARQRD FHPFAAARSE KRHADRRKHV
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgc cggccgcccg gcagcgtgac tttcacccgt tcgccgcggc gcgctccgag aagcgccatg ccgaccggcg aaaacacgtt taaccagccg tgctcgaaat tggccgcgtc cgggtagacg atggtccagg tcatgtcctg gccggcagtc tcgtcgagca gcttgatctg cgagttcatc gtgacgacgt ttgcctgaat cttgtcgggt tcgacgatat cggcgcgctc gagcagcgta tcgagcattt cctgatagtg cggattgcgt tccgcatgct tttcaaggcg cgcgacgtcg agttcggtaa gttggtaaat cctgttcttc atgacagtct ccaaatgatc ggcttgggtg gcgtggccaa cctgcgggat cgcccggagc ctgactgact cggctccagg atggaggctg ctgtcagatc aagcgccggg ccgcggcgag cggcgctgca agcgggcttc gaaggagggg gcgcgtcgcc gccgcgacca cggccgcacg cgaaccgcac atgcggtgcg caagaaaggc gtggaaggtc ggcgtgcgca taacgaggcg atgaaacgaa aggcaaaaga cggggcaaac tttacnaata tacaacnnnc gaccatttag acagccggca gacggttctg cgaatcgatt ttccattgtt gctgctatgg cgcttttaac cggctttcgg tcgaaattaa ccggctttcg gtcgaaatac agtccgccat ttttatcatt ggcgcnacgc tgtatgtcta aagta
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVPAARRDK IRPFGLCRPP PAIFLSVQKI LIVRVSSLGD VVHNMPVIAD IRRRHPDAQI DWLVEEGFAD LVRLVDGVRN VLPFSLRRWR KRLSASQTWR EIRAFRRRLA EQRYDLVIDC QGLIKTAWVA SWARGPLVGL GNRTDGAGYE WPVRFFYDKR VPIAPRTHVV ERSRQLVAAA LGDPAPAPDD PIDFGLDTHG AARALASLDL NLPVPYVVFV HATSRADKQW PDDAWIGLGE ALVRRGASLV LPWGSDAERA TSERLAKAFG AAAIVPPKLS LPAVVGLIDG AAATVGVDTG LVHIAAALKR PTVELYNFAT AWRTGGYWSA NVVNLGTAGA PPSLSQTKDA LASFGLL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgccggcc gcccggcgcg ataaaatccg tcctttcggt ctgtgccggc cgccgccggc catttttctc agcgtgcaaa aaattctgat cgtgcgcgtg tcgtcgctcg gcgacgtcgt gcataacatg ccggtgatcg ccgatattcg ccggcgccat cccgatgcgc agatcgactg gctcgtcgag gaaggcttcg ccgatctcgt gcggctcgtc gacggcgtgc gcaacgtgct gccgttctcg ctgcggcgct ggcgcaagcg cctgagcgca tcgcagacgt ggcgcgagat ccgcgcattc cggcggcgtc tcgccgaaca gcgctacgac ctcgtgatcg actgccaggg gctcatcaag accgcgtggg tcgcgagctg ggcgcgcggg ccgctcgtcg gcctcggcaa ccgcaccgac ggcgccggct acgaatggcc ggtgcgcttc ttctacgaca agcgcgtgcc gatcgcgccg cgcacgcacg tcgtcgagcg ctcgcggcaa ctcgtcgcgg cggcgctggg agaccccgcg ccggcgcccg acgatccgat cgacttcggc ctcgacacgc acggcgcggc gcgcgcgctc gcgtcgctcg atttgaacct gccggtgcct tacgtcgtgt tcgtgcatgc gacgtcgcgc gccgacaagc agtggcccga cgacgcgtgg atcggcctgg gcgaggcgct cgtgcggcgc ggcgcgtcgc tcgtgctgcc gtggggcagc gacgccgagc gcgcgacgag cgagcggctc gcgaaggcgt tcggcgcggc ggcgatcgtg ccgccgaagc tgtcgctgcc cgcggtcgtc ggcttgatcg acggtgcggc cgcgacggtc ggcgtcgata ccggcctcgt ccacatcgcg gcggcgctca agcgcccgac cgtcgaactg tacaatttcg cgacagcctg gcgcacgggc ggctactggt cggccaacgt cgtcaacctc ggcaccgccg gcgcgccgcc atccctttcg cagacgaagg acgccctcgc gtcgttcggc ctcttgtaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa