ButhA.17333.a

Aminotransferase, DegT/DnrJ/EryC1/StrS family (BTH_I2193)

CENTER ID: ButhA.17333.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.17333.a.A1.GE32829 full length 1 383
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.5012
RefSeq: YP_442714.1
UniProt: Q2SWI5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSQTSVPFLP FVKPEIDEET IQGVADVLRS GWITTGPQCQ RFEAALSEYC GGRPVRVFNS GTATLEIGLR IAGVGPGDEV ITTPASWVAT SNVILETGAT PVFVDIDPAT RNLDLDLVEK AITPRTKALI PVYLAGLPVD MDRLYAIARA HQLRVIEDAA QALGSTWRGQ RIGAIGDIVS FSFHPNKNIT SIEGGALVLN DEAEATLAQK YRLQGITRTG LDGMDCDVLG GKYNLTDVAA RVGWGQLAHL ERFNATRRTL ARRYFDAFAG GPALALGVGL PVADFEHGNW HMFQITLPLE KLSISRGEFM EQLKQRGIGS GVHYPAIHLF TLYRARGFKE GMFPHAERFG ASTVTLPLFT QMREADVQRV VDAVNQICEQ YGK
NT Sequence
atgagccaga cttccgttcc tttcctgccg ttcgtcaaac ccgagatcga cgaggaaacc atccagggcg tggccgacgt gctgcgctcc ggctggatca cgaccggccc gcagtgccag cgcttcgagg cagcgctgtc cgagtattgc ggcgggcgtc ccgtgcgggt gttcaactcc ggcacggcga cgctcgagat cggcctgcgg atcgcgggcg taggccccgg cgacgaagtg atcacgacgc ccgcgtcgtg ggtcgcgacg agcaacgtga tcctcgagac gggcgcgacg cccgtgttcg tcgacatcga tcccgcgacg cgcaacctcg atctcgatct cgtcgaaaag gcgatcacgc cgcgcacgaa ggcgctcatc ccggtgtacc tcgcgggcct gcccgtcgac atggaccgcc tctatgcgat cgcgcgcgcg catcagcttc gcgtgatcga ggacgccgcg caggcgctcg gctcgacgtg gcgcggccag cgcatcgggg cgatcggcga catcgtgtcg ttcagcttcc atccgaacaa gaacatcacg tcgatcgaag gcggcgcgct cgtgctcaac gacgaagccg aggcgacgct cgcgcagaag taccggctgc agggcatcac gcgcacgggg ctcgacggca tggactgcga cgtgctcggc ggcaagtaca atctgaccga cgtcgccgcg cgcgtcggct ggggccagct cgcgcatctc gaacgcttca acgcgacgcg ccgcacgctt gcgcgccgct acttcgacgc gttcgcgggc ggcccggcgc tcgcgctcgg cgtcgggctg cccgtcgcgg atttcgagca cggcaactgg cacatgttcc agatcacgct gccgctcgag aagctgtcga tctcgcgcgg cgagttcatg gagcagttga agcaacgcgg catcggctcg ggcgtccatt acccggccat tcaccttttc acgctgtacc gcgcgcgcgg cttcaaggag ggcatgttcc cccacgccga gcgcttcggc gcgtcgaccg tcacgctgcc gctcttcacg cagatgcgcg aagcggacgt gcaacgggtc gtcgacgccg tcaaccagat ttgcgaacaa tacggaaaat aa
Details for ButhA.17333.a.A1.GE32829
HARVESTED ON: 6/28/2011
SEQUENCED ON: 7/22/2011
EXPECTED MW: 44kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 82
PERCENT COVERAGE: 94
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSQTSVPFL PFVKPEIDEE TIQGVADVLR SGWITTGPQC QRFEAALSEY CGGRPVRVFN SGTATLEIGL RIAGVGPGDE VITTPASWVA TSNVILETGA TPVFVDIDPA TRNLDLDLVE KAITPRTKAL IPVYLAGLPV DMDRLYAIAR AHQLRVIEDA AQALGSTWRG QRIGAIGDIV SFSFHPNKNI TSIEGGALVL NDEAEATLAQ KYRLQGITRT GLDGMDCDVL GGKYNLTDVA ARVGWGQLAH LERFNATRRX LARRYFDAFA GGPALALGVG LPVAXXEXGN WXMXXITLPL XXCRSRXAXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXTXXXXX XXXXXXXXXP XX
Validated NT Sequence
attttgtnnn ntttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagcc agacttccgt tcctttcctg ccgttcgtca aacccgagat cgacgaggaa accatccagg gcgtggccga cgtgctgcgc tccggctgga tcacgaccgg cccgcagtgc cagcgcttcg aggcagcgct gtccgagtat tgcggcgggc gtcccgtgcg ggtgttcaac tccggcacgg cgacgctcga gatcggcctg cggatcgcgg gcgtaggccc cggcgacgaa gtgatcacga cgcccgcgtc gtgggtcgcg acgagcaacg tgatcctcga gacgggcgcg acgcccgtgt tcgtcgacat cgatcccgcg acgcgcaacc tcgatctcga tctcgtcgaa aaggcgatca cgccgcgcac gaaggcgctc atcccggtgt acctcgcggg cctgcccgtc gacatggacc gcctctatgc gatcgcgcgc gcgcatcagc ttcgcgtgat cgaggacgcc gcgcaggcgc tcggctcgac gtggcgcggc cagcgcatcg gggcgatcgg cgacatcgtg tcgttcagct tccatccgaa caagaacatc acgtcgatcg aaggcggcgc gctcgtgctc aacgacgaag ccgaggcgac gctcgcgcag aagtaccggc tgcagggcat cacgcgcacg gggctcgacg gcatggactg cgacgtgctc ggcggcaagt acaatctgac cgacgtcgcc gcgcgcgtcg gctggggcca gctcgcgcat ctcgaacgct tcaacgcgac gcgccgcang cttgcgcgcc gctacttcga cgcgttcgcg ggcggnccgg cgctcgcnct cggcgtcggg ctgcccgtcg cggnttncga gcanggcaac tggnacatgt tncanatcac gctgccgctc naganctgtc gatcncgcgn ggcgagnncn tnnnnantnn nnannngnat nnnnnnnnnt cnnnncnnnn tnncnttnnn ncnnnncnnn ngnngntnnn nnnnnnnnnc cnnnnnnann nnnnnnnnnn nnacnnnnnn nnntnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnccn nnnntnnna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSQTSVPFL PFVKPEIDEE TIQGVADVLR SGWITTGPQC QRFEAALSEY CGGRPVRVFN SGTATLEIGL RIAGVGPGDE VITTPASWVA TSNVILETGA TPVFVDIDPA TRNLDLDLVE KAITPRTKAL IPVYLAGLPV DMDRLYAIAR AHQLRVIEDA AQALGSTWRG QRIGAIGDIV SFSFHPNKNI TSIEGGALVL NDEAEATLAQ KYRLQGITRT GLDGMDCDVL GGKYNLTDVA ARVGWGQLAH LERFNATRRT LARRYFDAFA GGPALALGVG LPVADFEHGN WHMFQITLPL EKLSISRGEF MEQLKQRGIG SGVHYPAIHL FTLYRARGFK EGMFPHAERF GASTVTLPLF TQMREADVQR VVDAVNQICE QYGK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagccagact tccgttcctt tcctgccgtt cgtcaaaccc gagatcgacg aggaaaccat ccagggcgtg gccgacgtgc tgcgctccgg ctggatcacg accggcccgc agtgccagcg cttcgaggca gcgctgtccg agtattgcgg cgggcgtccc gtgcgggtgt tcaactccgg cacggcgacg ctcgagatcg gcctgcggat cgcgggcgta ggccccggcg acgaagtgat cacgacgccc gcgtcgtggg tcgcgacgag caacgtgatc ctcgagacgg gcgcgacgcc cgtgttcgtc gacatcgatc ccgcgacgcg caacctcgat ctcgatctcg tcgaaaaggc gatcacgccg cgcacgaagg cgctcatccc ggtgtacctc gcgggcctgc ccgtcgacat ggaccgcctc tatgcgatcg cgcgcgcgca tcagcttcgc gtgatcgagg acgccgcgca ggcgctcggc tcgacgtggc gcggccagcg catcggggcg atcggcgaca tcgtgtcgtt cagcttccat ccgaacaaga acatcacgtc gatcgaaggc ggcgcgctcg tgctcaacga cgaagccgag gcgacgctcg cgcagaagta ccggctgcag ggcatcacgc gcacggggct cgacggcatg gactgcgacg tgctcggcgg caagtacaat ctgaccgacg tcgccgcgcg cgtcggctgg ggccagctcg cgcatctcga acgcttcaac gcgacgcgcc gcacgcttgc gcgccgctac ttcgacgcgt tcgcgggcgg cccggcgctc gcgctcggcg tcgggctgcc cgtcgcggat ttcgagcacg gcaactggca catgttccag atcacgctgc cgctcgagaa gctgtcgatc tcgcgcggcg agttcatgga gcagttgaag caacgcggca tcggctcggg cgtccattac ccggccattc accttttcac gctgtaccgc gcgcgcggct tcaaggaggg catgttcccc cacgccgagc gcttcggcgc gtcgaccgtc acgctgccgc tcttcacgca gatgcgcgaa gcggacgtgc aacgggtcgt cgacgccgtc aaccagattt gcgaacaata cggaaaataa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa