ButhA.17253.b

Phospholipase C

CENTER ID: ButhA.17253.b
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.17253.b.A2.GE34857 Mature protein( ButhA.17253.b ) 51 749
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.88
RefSeq: YP_438280.1
UniProt: Q2T969

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRASPVHRSV NSLQGLRNMN SRRRFLQGTA STGIAAATLA AFPLSIRRAL AIPANNRTGT IRDVEHIVIL MQENRSFDNY FGTLRGVRGF GDRFGIPLPN ALTVWQQRNA AGALVLPYHL DGSKGNAQRV SGTPHSWDDG QNAWDGGRMY QWPRYKNAAS MGYFRESELP FQFALANAFT ICDAYHCSMH AGTNSNRMFM WTGTNGPTGA GVASVVNEWD DIGPSALGYE WKTYPERLQE AGVSWKVYQN MPDNFTDNSL AGFKQYRRAN EASGKPVSNS DKVVSPAYDP ASDDVRNPLY KGIANTMPDG GFLGTFKEDI RNGKLPQVSW VVAPAAYSEH PGPSSPVQGA WYIQETLDAL TAMPDVWSKT VLLVNFDEND GYFDHVPSPS APSLNPDGTP AGKTTLPDAD IAFERFIHPK PPGAKSQPQP DARVYGPGVR VPMYVISPWS RGGWVNSQVF DHTSTLRFIE ARFGVRELNI SAFRRAVCGD LTSAFNFVQP NNEPLPTLAG RKTRAQADAL RAAQQQMPQI VPPANGQLPR QAAGTRPSRA LPYELHVHAV AGTLDGTMRL AFVNTGRAAA VFHVYDKLNL GRLPRRYMVE AGKQLDDAWA SMTDNGGRYD LWVLGPNGFH RHFTGDMNRL RAGGAPVPEV RVEYDRKHGD VQLLLRNEGA RECVFTVRAK AYRSDGPWTF KVKGGAERKH HWKLDGSGNW YDFVVTCDAD AGYARRFAGR VETGEHSVSD PAMGFGDRF
NT Sequence
GTGCGCGCAT CGCCGGTTCA TCGCTCGGTC AATTCACTGC AAGGACTCCG GAACATGAAC TCTCGTCGCC GATTTCTGCA AGGCACCGCA TCCACGGGCA TCGCGGCCGC CACGCTGGCC GCATTCCCGC TCAGCATTCG CCGCGCGCTC GCGATTCCCG CGAACAACCG GACCGGAACC ATTCGGGACG TCGAGCACAT CGTCATCCTG ATGCAGGAGA ACCGTTCGTT CGACAACTAC TTCGGCACGC TGCGCGGCGT GCGCGGCTTC GGCGATCGCT TCGGCATTCC GCTGCCCAAC GCGCTGACGG TCTGGCAGCA GCGCAACGCG GCAGGCGCGC TGGTGTTGCC TTACCACCTG GACGGCAGCA AGGGCAACGC GCAGCGCGTG AGCGGCACGC CGCACTCGTG GGACGACGGC CAGAATGCGT GGGACGGCGG GCGCATGTAC CAATGGCCGC GCTACAAGAA TGCGGCGTCG ATGGGCTACT TCCGCGAGAG CGAGCTGCCG TTCCAGTTCG CGCTCGCGAA TGCGTTCACG ATCTGCGACG CCTACCACTG CTCGATGCAC GCGGGCACCA ACTCCAATCG CATGTTCATG TGGACCGGCA CCAACGGCCC GACCGGCGCG GGCGTGGCCT CGGTGGTGAA CGAGTGGGAC GACATCGGCC CCTCCGCGCT CGGCTACGAG TGGAAGACCT ATCCCGAGCG TCTGCAGGAA GCCGGGGTGA GCTGGAAGGT CTACCAGAAC ATGCCCGACA ACTTCACCGA CAACTCGCTC GCCGGCTTCA AGCAATACCG CCGCGCGAAC GAGGCGTCGG GCAAGCCGGT GAGCAACAGC GACAAGGTGG TGTCGCCCGC ATACGATCCG GCGAGCGACG ACGTTCGCAA CCCGCTGTAC AAGGGCATCG CCAACACGAT GCCCGACGGC GGCTTCCTCG GCACGTTCAA GGAAGACATC CGCAACGGCA AGCTGCCGCA GGTGTCGTGG GTGGTGGCGC CGGCCGCGTA TTCCGAGCAC CCTGGGCCGT CGAGCCCGGT GCAGGGCGCT TGGTACATCC AGGAGACGCT CGATGCGCTC ACCGCCATGC CCGACGTCTG GAGCAAGACC GTGCTGCTCG TGAATTTCGA CGAGAACGAC GGCTACTTCG ATCACGTGCC TTCGCCGTCG GCGCCGTCGC TGAACCCGGA CGGCACGCCG GCCGGCAAGA CCACGCTGCC CGACGCCGAC ATCGCGTTCG AGCGCTTCAT CCATCCGAAG CCGCCGGGCG CGAAGAGCCA GCCGCAGCCC GACGCTCGCG TGTATGGCCC CGGCGTGCGC GTGCCGATGT ACGTGATCTC GCCGTGGAGC CGCGGCGGCT GGGTCAACTC GCAGGTATTC GACCATACGT CGACGCTGCG CTTCATCGAG GCGCGCTTCG GCGTTCGCGA ACTCAATATT TCCGCGTTCC GCCGCGCGGT ATGCGGCGAC CTGACGAGCG CCTTCAACTT CGTGCAGCCG AACAACGAGC CGTTGCCCAC GCTCGCCGGC CGCAAGACCC GCGCGCAGGC CGATGCGCTG CGCGCCGCGC AGCAGCAGAT GCCGCAGATC GTGCCGCCCG CGAACGGGCA ACTGCCGCGC CAGGCGGCGG GCACGCGGCC TTCGCGCGCA TTGCCGTACG AACTGCACGT TCATGCCGTA GCCGGCACGC TCGACGGCAC GATGCGCCTC GCATTCGTCA ACACGGGCCG GGCCGCGGCC GTGTTCCACG TCTACGACAA GCTCAATCTG GGCCGCCTGC CGCGCCGCTA CATGGTCGAG GCCGGCAAGC AGCTCGACGA CGCGTGGGCC TCGATGACGG ACAACGGCGG CCGGTACGAC CTGTGGGTGC TCGGGCCGAA CGGCTTCCAC CGCCACTTCA CGGGCGACAT GAACCGCTTG CGCGCCGGCG GCGCGCCCGT GCCCGAAGTG CGCGTGGAGT ACGACCGCAA GCACGGCGAC GTGCAATTGC TGTTGCGTAA CGAAGGGGCG CGCGAGTGCG TGTTCACCGT GCGCGCGAAG GCGTACCGCA GCGATGGCCC GTGGACCTTC AAGGTCAAGG GCGGCGCCGA GCGCAAGCAT CACTGGAAGC TGGACGGCAG CGGCAACTGG TACGACTTCG TCGTGACCTG CGATGCCGAC GCCGGCTACG CGCGCCGTTT TGCCGGCCGG GTGGAAACCG GCGAGCACTC CGTCAGCGAT CCGGCGATGG GGTTCGGCGA TCGGTTC
Details for ButhA.17253.b.A2.GE34857
HARVESTED ON: 4/17/2012
SEQUENCED ON: 4/17/2012
EXPECTED MW: 79kDa
OBSERVED MW: 80kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 42
PERCENT COVERAGE: 28
Validated AA Sequence
MGTLEAQTQG PGSMAIPANN RTGTIXDVEH IVXLXXENRS FDNYFXXXRG VRGFXXXFXX PLPXXLTVWX XRNAAXXXXX XYHLDGSXXX XXCXXPXMPX PATRAVLPXG WKXAXXPXXX XXWXXAIGSX XQHXQXLXXX FSRIXLLXXX XXXXXXXXQS HVHVDRHXRP XXXXXXLXGE XVXXXXXLRA RLXXRXIPXX XXKPG
Validated NT Sequence
ttnnnnnnnt taagaaggag anataccntg gctcatcacc atcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atggcgattc ccgcgaacaa ccggaccgga accattcngg acgtcgagca catcgtcntc ctgnngnang agaaccgttc gttcgacaac tacttcgnnn nnntgcgcgg cgtgcgcggc ttcngnnann gcttcnncnt tccgctgccc nnnnngctga cggtctggnn acnacgcaac gcggcnngnn cgntgntgtn gcnttaccac ctggacggca gcnnnancng nnannactgc nnnngncctg ngatgccnan nccggctacg cgcgcngttt tgccggnngg gtggaaacng gcnagnnntc cgnnnnnnnn nnnnnnntgg gngncggcga tcggntctna taancagcac naacaanttc tgcngncnan cttctcnagg atccngctgc tannnnnnnc nnnnngannn nnnnngnnag anntccaatc ncatgttcat gtggaccggc accnacggcc cganngncnc gnnngtgnnc tcngtggnga acnagtggna cnacntcngn ccctccgcgc tcggctacnn nnnagancta tcccnanngn ctgnnngaag ccgggntgan ntggaangtc tacnanaaca tgcccnacaa cttcnccnac nactcgctcg ccggcttcna gcaataccgn cgcgcgaacg angcgtcngg naagccggtg agcnacacna cnangtggng tcgnncgcnt acnannnngc nangnnnnac gntcacnnnc tntacnanng ncatcnccaa cncnatgncc nacngcngcn gnncnnnnaa ngannncatn nnnnncggca anntgcnnnn nngtcgtggn gnggngncnn cnnnnantnn cnannaccnn ggnnngtcnn nnnnntgnnn nctnnnnnnn ncngnnnang ctcnatgnnn nnncnncntn nnnnnncgtc ngnngcagac ntgctgcnnn nnnnnnnnng acnannngnn nnnanttcga tnnnnnnnnn nnngnnnnnc nnnnnnnnnn nnnngnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAIPANNRT GTIRDVEHIV ILMQENRSFD NYFGTLRGVR GFGDRFGIPL PNALTVWQQR NAAGALVLPY HLDGSKGNAQ RVSGTPHSWD DGQNAWDGGR MYQWPRYKNA ASMGYFRESE LPFQFALANA FTICDAYHCS MHAGTNSNRM FMWTGTNGPT GAGVASVVNE WDDIGPSALG YEWKTYPERL QEAGVSWKVY QNMPDNFTDN SLAGFKQYRR ANEASGKPVS NSDKVVSPAY DPASDDVRNP LYKGIANTMP DGGFLGTFKE DIRNGKLPQV SWVVAPAAYS EHPGPSSPVQ GAWYIQETLD ALTAMPDVWS KTVLLVNFDE NDGYFDHVPS PSAPSLNPDG TPAGKTTLPD ADIAFERFIH PKPPGAKSQP QPDARVYGPG VRVPMYVISP WSRGGWVNSQ VFDHTSTLRF IEARFGVREL NISAFRRAVC GDLTSAFNFV QPNNEPLPTL AGRKTRAQAD ALRAAQQQMP QIVPPANGQL PRQAAGTRPS RALPYELHVH AVAGTLDGTM RLAFVNTGRA AAVFHVYDKL NLGRLPRRYM VEAGKQLDDA WASMTDNGGR YDLWVLGPNG FHRHFTGDMN RLRAGGAPVP EVRVEYDRKH GDVQLLLRNE GARECVFTVR AKAYRSDGPW TFKVKGGAER KHHWKLDGSG NWYDFVVTCD ADAGYARRFA GRVETGEHSV SDPAMGFGDR F
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgattccc gcgaacaacc ggaccggaac cattcgggac gtcgagcaca tcgtcatcct gatgcaggag aaccgttcgt tcgacaacta cttcggcacg ctgcgcggcg tgcgcggctt cggcgatcgc ttcggcattc cgctgcccaa cgcgctgacg gtctggcagc agcgcaacgc ggcaggcgcg ctggtgttgc cttaccacct ggacggcagc aagggcaacg cgcagcgcgt gagcggcacg ccgcactcgt gggacgacgg ccagaatgcg tgggacggcg ggcgcatgta ccaatggccg cgctacaaga atgcggcgtc gatgggctac ttccgcgaga gcgagctgcc gttccagttc gcgctcgcga atgcgttcac gatctgcgac gcctaccact gctcgatgca cgcgggcacc aactccaatc gcatgttcat gtggaccggc accaacggcc cgaccggcgc gggcgtggcc tcggtggtga acgagtggga cgacatcggc ccctccgcgc tcggctacga gtggaagacc tatcccgagc gtctgcagga agccggggtg agctggaagg tctaccagaa catgcccgac aacttcaccg acaactcgct cgccggcttc aagcaatacc gccgcgcgaa cgaggcgtcg ggcaagccgg tgagcaacag cgacaaggtg gtgtcgcccg catacgatcc ggcgagcgac gacgttcgca acccgctgta caagggcatc gccaacacga tgcccgacgg cggcttcctc ggcacgttca aggaagacat ccgcaacggc aagctgccgc aggtgtcgtg ggtggtggcg ccggccgcgt attccgagca ccctgggccg tcgagcccgg tgcagggcgc ttggtacatc caggagacgc tcgatgcgct caccgccatg cccgacgtct ggagcaagac cgtgctgctc gtgaatttcg acgagaacga cggctacttc gatcacgtgc cttcgccgtc ggcgccgtcg ctgaacccgg acggcacgcc ggccggcaag accacgctgc ccgacgccga catcgcgttc gagcgcttca tccatccgaa gccgccgggc gcgaagagcc agccgcagcc cgacgctcgc gtgtatggcc ccggcgtgcg cgtgccgatg tacgtgatct cgccgtggag ccgcggcggc tgggtcaact cgcaggtatt cgaccatacg tcgacgctgc gcttcatcga ggcgcgcttc ggcgttcgcg aactcaatat ttccgcgttc cgccgcgcgg tatgcggcga cctgacgagc gccttcaact tcgtgcagcc gaacaacgag ccgttgccca cgctcgccgg ccgcaagacc cgcgcgcagg ccgatgcgct gcgcgccgcg cagcagcaga tgccgcagat cgtgccgccc gcgaacgggc aactgccgcg ccaggcggcg ggcacgcggc cttcgcgcgc attgccgtac gaactgcacg ttcatgccgt agccggcacg ctcgacggca cgatgcgcct cgcattcgtc aacacgggcc gggccgcggc cgtgttccac gtctacgaca agctcaatct gggccgcctg ccgcgccgct acatggtcga ggccggcaag cagctcgacg acgcgtgggc ctcgatgacg gacaacggcg gccggtacga cctgtgggtg ctcgggccga acggcttcca ccgccacttc acgggcgaca tgaaccgctt gcgcgccggc ggcgcgcccg tgcccgaagt gcgcgtggag tacgaccgca agcacggcga cgtgcaattg ctgttgcgta acgaaggggc gcgcgagtgc gtgttcaccg tgcgcgcgaa ggcgtaccgc agcgatggcc cgtggacctt caaggtcaag ggcggcgccg agcgcaagca tcactggaag ctggacggca gcggcaactg gtacgacttc gtcgtgacct gcgatgccga cgccggctac gcgcgccgtt ttgccggccg ggtggaaacc ggcgagcact ccgtcagcga tccggcgatg gggttcggcg atcggttcta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a