ButhA.17253.a

Non-hemolytic phospholipase C

CENTER ID: ButhA.17253.a
ORGANISM: Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.17253.a.A1.GE34858 Full length( ButhA.17253.a ) 1 742
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ButhA.17253.a.A1.PS01564 Full length( ButhA.17253.a ) 1 742 unavailable

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|271848.6.peg.2977
RefSeq: YP_440875.1
UniProt: Q2T1S4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAPTTPPAQR RRPRNPPRIQ ADRFLPPPLP RRVKGENMTS ESRRRFLHAV AQSASAAAAL TVFPESIRRA LAIPAASRTG TIRDVEHIVV FMQENRSFDH YFGHLRGVRG YNDRFPITLP NGKPVWYQPS KANPGKPVLP FRLNTLTTSA QCIGDLDHSW YKTHAAIDGG RYDQWPANKT DMTMGYHVRE DIPFHYALAD AFTVCDNYFC SLPGPTHPNR SYLMTGTVDP TGKYGGPLLD NSDYVDGDAP PKYDLLTWTT YPERLEANGI SWQIYQQGTT GADPLNGNYG TNILQNFANF IDAKPGSSLY RRAQTARTLD DLKADVLANR LPQVSWLLPP AAFSEHPKYT PAYGANYTSQ ILDALTSNPD VWRKTVLFIM YDENDGFFDH VVPPQPATTR AQGLSTVPVD GEIHDVVNPG RGGSYTADGL PYGLGPRVPM TVVSPWTKGG FVCSQVFDHT SVIRFIAARF GVDEPNITPW RRAVCGDLTS AFDFRTPDAT LPPLPDTSNY RSIADQLCST KPAPSVPAAP SPVDPQEPGV RPARALPYEL HVNATVHGGA RLRIEFANRG EQGAHFYVYA SNRSDGPWRY TVGAHRSLHD EFDLGATNGA YAFTVYGPNG FVRVFEGLAA AEGHGRHAAA HPEVKAGYDV ANGNLYLKLT NHGGQSVRLT LADNAYGAPP RQITLRGGDE RVEQWALAPS HHWYDVTVSD GATGQFVRRF AGHVENGKSS YSDPAAVAPV TG
NT Sequence
ATGGCGCCGA CGACGCCCCC GGCGCAACGG CGGCGTCCGC GCAACCCGCC TCGAATCCAG GCTGATCGCT TTTTGCCGCC CCCTCTTCCC CGCAGAGTCA AAGGTGAAAA CATGACGTCA GAAAGCCGCC GCCGTTTCCT GCATGCCGTT GCGCAATCCG CGAGCGCCGC CGCCGCGCTC ACCGTGTTTC CCGAATCGAT CCGCCGCGCG CTCGCGATTC CCGCCGCGTC GCGCACGGGC ACGATCCGCG ATGTCGAGCA CATCGTCGTG TTCATGCAGG AAAACCGCTC GTTCGACCAC TACTTCGGAC ACCTGCGCGG CGTGCGCGGC TACAACGACC GCTTCCCGAT CACGCTGCCG AACGGCAAAC CGGTCTGGTA TCAGCCGTCG AAGGCGAACC CGGGCAAGCC GGTGCTGCCG TTTCGCCTGA ACACGCTGAC GACGAGCGCG CAGTGCATCG GCGACCTCGA TCACTCGTGG TACAAGACGC ACGCGGCGAT CGACGGCGGC CGCTACGACC AGTGGCCCGC GAACAAGACC GACATGACGA TGGGCTATCA CGTGCGCGAG GACATTCCGT TCCACTACGC GCTCGCCGAT GCATTCACCG TCTGCGACAA CTACTTCTGC TCGCTGCCCG GCCCGACGCA TCCGAACCGC TCGTATCTGA TGACGGGCAC CGTCGACCCA ACCGGCAAGT ACGGCGGCCC GCTTCTCGAC AACAGCGATT ACGTCGACGG CGACGCGCCG CCGAAATACG ACTTGCTCAC GTGGACCACG TACCCCGAGC GCCTCGAGGC GAACGGCATC TCGTGGCAGA TCTACCAGCA AGGCACGACG GGCGCCGATC CGCTGAACGG CAACTACGGG ACGAACATCC TGCAGAACTT CGCGAACTTC ATCGACGCGA AGCCGGGCTC GTCGCTGTAT CGGCGCGCGC AGACGGCGCG CACGCTCGAC GACCTGAAGG CCGACGTGCT CGCGAACAGG CTGCCGCAGG TGTCGTGGCT GCTGCCGCCC GCGGCATTCT CCGAGCATCC GAAATACACG CCCGCGTACG GCGCGAACTA CACGTCGCAA ATCCTCGACG CGCTGACGTC GAACCCCGAC GTGTGGCGCA AGACCGTGCT CTTCATCATG TACGACGAGA ACGACGGCTT CTTCGATCAC GTCGTGCCGC CGCAGCCGGC GACGACGCGC GCGCAGGGCC TGTCGACCGT GCCCGTCGAC GGCGAGATCC ACGACGTCGT GAACCCGGGC CGCGGCGGCA GCTACACGGC CGACGGCCTG CCGTACGGCC TCGGCCCGCG CGTGCCGATG ACGGTCGTGT CGCCGTGGAC GAAGGGCGGC TTCGTGTGCT CGCAGGTGTT CGACCATACG TCGGTGATCC GCTTCATCGC CGCGCGCTTC GGCGTCGACG AGCCGAACAT CACGCCGTGG CGCCGCGCGG TGTGCGGCGA CCTCACGTCC GCGTTCGACT TCCGCACGCC GGACGCGACA CTGCCGCCGC TGCCCGACAC GAGCAACTAT CGGTCGATCG CCGACCAGCT CTGCTCGACG AAGCCCGCGC CGAGCGTGCC CGCCGCGCCG AGTCCCGTCG ATCCGCAGGA GCCCGGCGTG CGCCCGGCGC GCGCGCTGCC GTACGAGCTG CACGTGAACG CGACGGTGCA CGGCGGCGCG CGCTTGCGCA TCGAGTTCGC GAATCGCGGC GAACAGGGCG CGCACTTCTA CGTGTACGCG TCGAATCGCA GCGACGGCCC GTGGCGCTAC ACGGTGGGCG CACACCGCTC GCTGCACGAC GAATTCGATC TCGGCGCGAC GAACGGCGCG TATGCGTTCA CCGTATATGG TCCGAACGGC TTCGTGCGCG TGTTCGAAGG GCTCGCCGCA GCCGAGGGGC ACGGCCGGCA TGCGGCCGCG CATCCGGAAG TGAAGGCGGG CTACGACGTC GCGAACGGCA ACCTGTATCT GAAGCTCACG AATCACGGCG GACAGAGCGT GCGACTGACG CTCGCCGACA ACGCGTACGG CGCGCCGCCG CGCCAGATCA CGCTGCGCGG CGGCGACGAG CGGGTGGAGC AATGGGCGCT TGCCCCGAGC CATCACTGGT ATGACGTGAC GGTGTCGGAC GGCGCGACCG GCCAGTTCGT GCGCCGCTTC GCCGGGCACG TCGAAAACGG CAAGTCGAGC TATTCCGATC CGGCAGCAGT CGCGCCCGTC ACGGGC
Details for ButhA.17253.a.A1.GE34858
HARVESTED ON: 4/17/2012
SEQUENCED ON: 4/17/2012
EXPECTED MW: 83kDa
OBSERVED MW: 80kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 93
PERCENT COVERAGE: 38
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAPTTPPAX RRRPRNPPRI XADRFLPPPL PRXVXXENMT SESRRRFLHA VAXSASAAAA LTVFPESIRR ALAIPAASRT GTIRDVEHIX XFMQENRSFD HYFGHLRGVX XXXXRFPITL PNGKPVWYQP SKANPGKPVL PFXLXTLTTS AQCIGDLDHS WYKTHAAIDG GRYDQWPANK TDMXMGYHVR EDIPFHYALA DAFTVCDNYF CSLPGPTHPN RSYLMTGTVD PTGKYGGPLL DNXDYVDGDA PPKYDLXTWT TYPERLEANG XSW
Validated NT Sequence
ttaagaagga gnnataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggcgccg acgacgcccc cggcgcancg gcggcgtccg cgcaacccgc ctcgaatccn ggctgatcgc tttttgccgc cccctcttcc ccgcanagtc nnnngtgaaa acatgacgtc agaaagccgc cgccgtttcc tgcatgccgt tgcgcantcc gcgagcgccg ccgccgcgct caccgtgttt cccgaatcga tccgccgcgc gctcgcgatt cccgccgcgt cgcgcacggg nacgatccgc gatgtcgagc acatcgncnt gttcatgcag gaaaaccgct cgttcgacca ctacttcgga cacctgcgcg gcgtnnnnnn ctannnnnnc cgcttcccga tcacgctgcc gaacggcaaa ccggtctggt atcagccgtc naaggcgaac ccgggcaagc cggtgctgcc gtttnncctg annacnctga cgacgagcgc gcagtgcatc ggcgacctcg atcactcgtg gtacaagacg cacgcggcga tcgacggcgg ccgctacgac cagtggcccg cgaacaagac cgacatgncg atgggctatc acgtgcgcga ggacattccg ttccactacg cgctcgccga tgcattcacc gtctgcgaca actacttctg ctcgctgccc ggnccgacgc atccgaaccg ctcgtatctg atgacgggca ccgtcgaccc naccggcaag tacggcggcc cgcttctcga caacngcgat tacgtcgacg gcgacgcgcc gccgaaatac gacttgntca cgtggaccac gtaccccgag cgcctcgagg cnaacggnnt ctcgtggna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAPTTPPAQ RRRPRNPPRI QADRFLPPPL PRRVKGENMT SESRRRFLHA VAQSASAAAA LTVFPESIRR ALAIPAASRT GTIRDVEHIV VFMQENRSFD HYFGHLRGVR GYNDRFPITL PNGKPVWYQP SKANPGKPVL PFRLNTLTTS AQCIGDLDHS WYKTHAAIDG GRYDQWPANK TDMTMGYHVR EDIPFHYALA DAFTVCDNYF CSLPGPTHPN RSYLMTGTVD PTGKYGGPLL DNSDYVDGDA PPKYDLLTWT TYPERLEANG ISWQIYQQGT TGADPLNGNY GTNILQNFAN FIDAKPGSSL YRRAQTARTL DDLKADVLAN RLPQVSWLLP PAAFSEHPKY TPAYGANYTS QILDALTSNP DVWRKTVLFI MYDENDGFFD HVVPPQPATT RAQGLSTVPV DGEIHDVVNP GRGGSYTADG LPYGLGPRVP MTVVSPWTKG GFVCSQVFDH TSVIRFIAAR FGVDEPNITP WRRAVCGDLT SAFDFRTPDA TLPPLPDTSN YRSIADQLCS TKPAPSVPAA PSPVDPQEPG VRPARALPYE LHVNATVHGG ARLRIEFANR GEQGAHFYVY ASNRSDGPWR YTVGAHRSLH DEFDLGATNG AYAFTVYGPN GFVRVFEGLA AAEGHGRHAA AHPEVKAGYD VANGNLYLKL TNHGGQSVRL TLADNAYGAP PRQITLRGGD ERVEQWALAP SHHWYDVTVS DGATGQFVRR FAGHVENGKS SYSDPAAVAP VTG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgccgacg acgcccccgg cgcaacggcg gcgtccgcgc aacccgcctc gaatccaggc tgatcgcttt ttgccgcccc ctcttccccg cagagtcaaa ggtgaaaaca tgacgtcaga aagccgccgc cgtttcctgc atgccgttgc gcaatccgcg agcgccgccg ccgcgctcac cgtgtttccc gaatcgatcc gccgcgcgct cgcgattccc gccgcgtcgc gcacgggcac gatccgcgat gtcgagcaca tcgtcgtgtt catgcaggaa aaccgctcgt tcgaccacta cttcggacac ctgcgcggcg tgcgcggcta caacgaccgc ttcccgatca cgctgccgaa cggcaaaccg gtctggtatc agccgtcgaa ggcgaacccg ggcaagccgg tgctgccgtt tcgcctgaac acgctgacga cgagcgcgca gtgcatcggc gacctcgatc actcgtggta caagacgcac gcggcgatcg acggcggccg ctacgaccag tggcccgcga acaagaccga catgacgatg ggctatcacg tgcgcgagga cattccgttc cactacgcgc tcgccgatgc attcaccgtc tgcgacaact acttctgctc gctgcccggc ccgacgcatc cgaaccgctc gtatctgatg acgggcaccg tcgacccaac cggcaagtac ggcggcccgc ttctcgacaa cagcgattac gtcgacggcg acgcgccgcc gaaatacgac ttgctcacgt ggaccacgta ccccgagcgc ctcgaggcga acggcatctc gtggcagatc taccagcaag gcacgacggg cgccgatccg ctgaacggca actacgggac gaacatcctg cagaacttcg cgaacttcat cgacgcgaag ccgggctcgt cgctgtatcg gcgcgcgcag acggcgcgca cgctcgacga cctgaaggcc gacgtgctcg cgaacaggct gccgcaggtg tcgtggctgc tgccgcccgc ggcattctcc gagcatccga aatacacgcc cgcgtacggc gcgaactaca cgtcgcaaat cctcgacgcg ctgacgtcga accccgacgt gtggcgcaag accgtgctct tcatcatgta cgacgagaac gacggcttct tcgatcacgt cgtgccgccg cagccggcga cgacgcgcgc gcagggcctg tcgaccgtgc ccgtcgacgg cgagatccac gacgtcgtga acccgggccg cggcggcagc tacacggccg acggcctgcc gtacggcctc ggcccgcgcg tgccgatgac ggtcgtgtcg ccgtggacga agggcggctt cgtgtgctcg caggtgttcg accatacgtc ggtgatccgc ttcatcgccg cgcgcttcgg cgtcgacgag ccgaacatca cgccgtggcg ccgcgcggtg tgcggcgacc tcacgtccgc gttcgacttc cgcacgccgg acgcgacact gccgccgctg cccgacacga gcaactatcg gtcgatcgcc gaccagctct gctcgacgaa gcccgcgccg agcgtgcccg ccgcgccgag tcccgtcgat ccgcaggagc ccggcgtgcg cccggcgcgc gcgctgccgt acgagctgca cgtgaacgcg acggtgcacg gcggcgcgcg cttgcgcatc gagttcgcga atcgcggcga acagggcgcg cacttctacg tgtacgcgtc gaatcgcagc gacggcccgt ggcgctacac ggtgggcgca caccgctcgc tgcacgacga attcgatctc ggcgcgacga acggcgcgta tgcgttcacc gtatatggtc cgaacggctt cgtgcgcgtg ttcgaagggc tcgccgcagc cgaggggcac ggccggcatg cggccgcgca tccggaagtg aaggcgggct acgacgtcgc gaacggcaac ctgtatctga agctcacgaa tcacggcgga cagagcgtgc gactgacgct cgccgacaac gcgtacggcg cgccgccgcg ccagatcacg ctgcgcggcg gcgacgagcg ggtggagcaa tgggcgcttg ccccgagcca tcactggtat gacgtgacgg tgtcggacgg cgcgaccggc cagttcgtgc gccgcttcgc cgggcacgtc gaaaacggca agtcgagcta ttccgatccg gcagcagtcg cgcccgtcac gggctaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for ButhA.17253.a.A1.PS01564
PURIFICATION DATe: 7/17/2012
CONCENTRATION: 49.1mg/ml
OBSERVED MW: 80kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): data unavailable
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 93
PERCENT COVERAGE: 38
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAPTTPPAX RRRPRNPPRI XADRFLPPPL PRXVXXENMT SESRRRFLHA VAXSASAAAA LTVFPESIRR ALAIPAASRT GTIRDVEHIX XFMQENRSFD HYFGHLRGVX XXXXRFPITL PNGKPVWYQP SKANPGKPVL PFXLXTLTTS AQCIGDLDHS WYKTHAAIDG GRYDQWPANK TDMXMGYHVR EDIPFHYALA DAFTVCDNYF CSLPGPTHPN RSYLMTGTVD PTGKYGGPLL DNXDYVDGDA PPKYDLXTWT TYPERLEANG XSW
Validated NT Sequence
ttaagaagga gnnataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggcgccg acgacgcccc cggcgcancg gcggcgtccg cgcaacccgc ctcgaatccn ggctgatcgc tttttgccgc cccctcttcc ccgcanagtc nnnngtgaaa acatgacgtc agaaagccgc cgccgtttcc tgcatgccgt tgcgcantcc gcgagcgccg ccgccgcgct caccgtgttt cccgaatcga tccgccgcgc gctcgcgatt cccgccgcgt cgcgcacggg nacgatccgc gatgtcgagc acatcgncnt gttcatgcag gaaaaccgct cgttcgacca ctacttcgga cacctgcgcg gcgtnnnnnn ctannnnnnc cgcttcccga tcacgctgcc gaacggcaaa ccggtctggt atcagccgtc naaggcgaac ccgggcaagc cggtgctgcc gtttnncctg annacnctga cgacgagcgc gcagtgcatc ggcgacctcg atcactcgtg gtacaagacg cacgcggcga tcgacggcgg ccgctacgac cagtggcccg cgaacaagac cgacatgncg atgggctatc acgtgcgcga ggacattccg ttccactacg cgctcgccga tgcattcacc gtctgcgaca actacttctg ctcgctgccc ggnccgacgc atccgaaccg ctcgtatctg atgacgggca ccgtcgaccc naccggcaag tacggcggcc cgcttctcga caacngcgat tacgtcgacg gcgacgcgcc gccgaaatac gacttgntca cgtggaccac gtaccccgag cgcctcgagg cnaacggnnt ctcgtggna
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAPTTPPAQ RRRPRNPPRI QADRFLPPPL PRRVKGENMT SESRRRFLHA VAQSASAAAA LTVFPESIRR ALAIPAASRT GTIRDVEHIV VFMQENRSFD HYFGHLRGVR GYNDRFPITL PNGKPVWYQP SKANPGKPVL PFRLNTLTTS AQCIGDLDHS WYKTHAAIDG GRYDQWPANK TDMTMGYHVR EDIPFHYALA DAFTVCDNYF CSLPGPTHPN RSYLMTGTVD PTGKYGGPLL DNSDYVDGDA PPKYDLLTWT TYPERLEANG ISWQIYQQGT TGADPLNGNY GTNILQNFAN FIDAKPGSSL YRRAQTARTL DDLKADVLAN RLPQVSWLLP PAAFSEHPKY TPAYGANYTS QILDALTSNP DVWRKTVLFI MYDENDGFFD HVVPPQPATT RAQGLSTVPV DGEIHDVVNP GRGGSYTADG LPYGLGPRVP MTVVSPWTKG GFVCSQVFDH TSVIRFIAAR FGVDEPNITP WRRAVCGDLT SAFDFRTPDA TLPPLPDTSN YRSIADQLCS TKPAPSVPAA PSPVDPQEPG VRPARALPYE LHVNATVHGG ARLRIEFANR GEQGAHFYVY ASNRSDGPWR YTVGAHRSLH DEFDLGATNG AYAFTVYGPN GFVRVFEGLA AAEGHGRHAA AHPEVKAGYD VANGNLYLKL TNHGGQSVRL TLADNAYGAP PRQITLRGGD ERVEQWALAP SHHWYDVTVS DGATGQFVRR FAGHVENGKS SYSDPAAVAP VTG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCGCCGACG ACGCCCCCGG CGCAACGGCG GCGTCCGCGC AACCCGCCTC GAATCCAGGC TGATCGCTTT TTGCCGCCCC CTCTTCCCCG CAGAGTCAAA GGTGAAAACA TGACGTCAGA AAGCCGCCGC CGTTTCCTGC ATGCCGTTGC GCAATCCGCG AGCGCCGCCG CCGCGCTCAC CGTGTTTCCC GAATCGATCC GCCGCGCGCT CGCGATTCCC GCCGCGTCGC GCACGGGCAC GATCCGCGAT GTCGAGCACA TCGTCGTGTT CATGCAGGAA AACCGCTCGT TCGACCACTA CTTCGGACAC CTGCGCGGCG TGCGCGGCTA CAACGACCGC TTCCCGATCA CGCTGCCGAA CGGCAAACCG GTCTGGTATC AGCCGTCGAA GGCGAACCCG GGCAAGCCGG TGCTGCCGTT TCGCCTGAAC ACGCTGACGA CGAGCGCGCA GTGCATCGGC GACCTCGATC ACTCGTGGTA CAAGACGCAC GCGGCGATCG ACGGCGGCCG CTACGACCAG TGGCCCGCGA ACAAGACCGA CATGACGATG GGCTATCACG TGCGCGAGGA CATTCCGTTC CACTACGCGC TCGCCGATGC ATTCACCGTC TGCGACAACT ACTTCTGCTC GCTGCCCGGC CCGACGCATC CGAACCGCTC GTATCTGATG ACGGGCACCG TCGACCCAAC CGGCAAGTAC GGCGGCCCGC TTCTCGACAA CAGCGATTAC GTCGACGGCG ACGCGCCGCC GAAATACGAC TTGCTCACGT GGACCACGTA CCCCGAGCGC CTCGAGGCGA ACGGCATCTC GTGGCAGATC TACCAGCAAG GCACGACGGG CGCCGATCCG CTGAACGGCA ACTACGGGAC GAACATCCTG CAGAACTTCG CGAACTTCAT CGACGCGAAG CCGGGCTCGT CGCTGTATCG GCGCGCGCAG ACGGCGCGCA CGCTCGACGA CCTGAAGGCC GACGTGCTCG CGAACAGGCT GCCGCAGGTG TCGTGGCTGC TGCCGCCCGC GGCATTCTCC GAGCATCCGA AATACACGCC CGCGTACGGC GCGAACTACA CGTCGCAAAT CCTCGACGCG CTGACGTCGA ACCCCGACGT GTGGCGCAAG ACCGTGCTCT TCATCATGTA CGACGAGAAC GACGGCTTCT TCGATCACGT CGTGCCGCCG CAGCCGGCGA CGACGCGCGC GCAGGGCCTG TCGACCGTGC CCGTCGACGG CGAGATCCAC GACGTCGTGA ACCCGGGCCG CGGCGGCAGC TACACGGCCG ACGGCCTGCC GTACGGCCTC GGCCCGCGCG TGCCGATGAC GGTCGTGTCG CCGTGGACGA AGGGCGGCTT CGTGTGCTCG CAGGTGTTCG ACCATACGTC GGTGATCCGC TTCATCGCCG CGCGCTTCGG CGTCGACGAG CCGAACATCA CGCCGTGGCG CCGCGCGGTG TGCGGCGACC TCACGTCCGC GTTCGACTTC CGCACGCCGG ACGCGACACT GCCGCCGCTG CCCGACACGA GCAACTATCG GTCGATCGCC GACCAGCTCT GCTCGACGAA GCCCGCGCCG AGCGTGCCCG CCGCGCCGAG TCCCGTCGAT CCGCAGGAGC CCGGCGTGCG CCCGGCGCGC GCGCTGCCGT ACGAGCTGCA CGTGAACGCG ACGGTGCACG GCGGCGCGCG CTTGCGCATC GAGTTCGCGA ATCGCGGCGA ACAGGGCGCG CACTTCTACG TGTACGCGTC GAATCGCAGC GACGGCCCGT GGCGCTACAC GGTGGGCGCA CACCGCTCGC TGCACGACGA ATTCGATCTC GGCGCGACGA ACGGCGCGTA TGCGTTCACC GTATATGGTC CGAACGGCTT CGTGCGCGTG TTCGAAGGGC TCGCCGCAGC CGAGGGGCAC GGCCGGCATG CGGCCGCGCA TCCGGAAGTG AAGGCGGGCT ACGACGTCGC GAACGGCAAC CTGTATCTGA AGCTCACGAA TCACGGCGGA CAGAGCGTGC GACTGACGCT CGCCGACAAC GCGTACGGCG CGCCGCCGCG CCAGATCACG CTGCGCGGCG GCGACGAGCG GGTGGAGCAA TGGGCGCTTG CCCCGAGCCA TCACTGGTAT GACGTGACGG TGTCGGACGG CGCGACCGGC CAGTTCGTGC GCCGCTTCGC CGGGCACGTC GAAAACGGCA AGTCGAGCTA TTCCGATCCG GCAGCAGTCG CGCCCGTCAC GGGCTAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA