ButhA.17230.b

1-deoxy-D-xylulose-5-phosphate synthase (EC 2.2.1.7) (1-deoxyxylulose-5-phosphate synthase) (DXP synthase) (DXPS) (BTH_II0614) (dxs)

CENTER ID:	ButhA.17230.b
ORGANISM:	Burkholderia thailandensis E264
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ButhA.17230.b.A1.GE32773	full length		1	634

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_129068
PATRIC ID:	fig\|271848.6.peg.699
RefSeq:	YP_438814.1
UniProt:	Q2T7N5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MYDLLKTIDD PADLRRLDRR QLQPLADELR AFVLDSVSKT GGHLSSNLGT VELTIALHYV FNTPDDRIVW DVGHQTYPHK ILTGRRDGMK TLRQFDGISG FPRRSESEYD TFGTAHSSTS ISAALGMAIG SKLNGDDRFS IAVIGDGAMT AGMAFEAMNN AGVSEDAKLL VILNDNDMSI SPPVGALNRH LARLMSGRFY AAARAGVERV LSVAPPVLEL ARKLEEHAKG MVVPATLFEE FGFNYIGPID GHDLDSLIPT LQNIKELRGP QFLHVVTKKG QGYKLAEADP VLYHGPGKFN PAEGIKPSAT PAKKTYTQVF GEWLCDAAEL DARVVGITPA MREGSGMVEF EKRFPERYYD VGIAEQHAVT FAGGLATEGL KPVVAIYSTF LQRAYDQLIH DVALQNLPVV FAIDRAGLVG ADGATHAGAY DLAFLRCIPN MTVMAASDEN ECRQMLHTAL QQPNPTAVRY PRGAGTGVAT VKAFTEIPLG KGEVRRRTSQ PDGKRVAILA FGTMVAPSLA AADALDATVA NMRFVKPIDA ELVRELAQTH DYLVTVEEGC VMGGAGSACV EAMMEGGAVR PVLQLGLPDR FVDHGDPAKL LAMCGLDGDG IAKSIRERFL NHAANVASPA KRVA

NT Sequence

atgtacgact tgctgaaaac catcgacgac ccggcggacc tgcgccgtct cgatcgtcgc caactgcagc cgctcgccga cgagctgcgc gcgttcgttc tcgacagcgt gtcgaagacg ggcggccatt tgtcgtccaa tctcggcacg gtcgagctga cgatcgcgct gcattacgtg ttcaacacgc cggacgaccg gatcgtgtgg gacgtcggtc accagaccta tccgcacaag atcctgacgg gccgccgcga cgggatgaag acgctgcgcc agttcgacgg catctcgggc tttccgcgcc gctcggaatc cgaatacgac acgttcggca ccgcgcactc gagcacgtcg atctcggctg cgctcgggat ggcgatcggc agcaagctga acggcgacga ccgcttctcg atcgcggtga tcggcgacgg cgcgatgacg gccggcatgg cgttcgaggc gatgaacaac gcgggcgtgt ccgaggacgc gaagctcctc gtgatcctga acgacaacga catgtcgatc tcgccgcccg tcggcgcgct gaaccgccat ctcgcgcgcc tgatgtcggg ccgcttctac gcggccgcgc gcgcgggcgt cgaacgcgtg ctgagcgtcg cgccgcccgt gctcgagctc gcgcgcaagc tcgaggagca tgcgaagggc atggtggtgc ccgcgacgct cttcgaggaa ttcggcttca actacatcgg gccgatcgac ggccacgatc tcgattcgct gatcccgacg ctgcagaaca tcaaggaact gcgcggcccg cagttcctgc acgtcgtgac gaagaaggga cagggctaca agctcgctga ggccgatccg gtgctctatc acgggcccgg caagttcaac ccggcggaag gcatcaagcc gtccgcgacg ccggcgaaga agacctacac gcaggtattc ggcgaatggc tgtgcgacgc ggcggagctc gatgcgcgcg tggtcggcat cacgcccgcg atgcgcgaag gctcgggcat ggtcgagttc gagaagcgct tcccggagcg ctactacgac gtcggcattg ccgagcagca cgcggtgacg ttcgcgggcg gcctcgcgac ggaagggctc aagcccgtcg tcgcgatcta ctcgactttc ctgcagcgcg cctatgatca gctgattcac gacgtcgcgc tgcagaacct gcccgtcgtg ttcgcgatcg atcgcgcggg cctcgtcggc gcggacggcg cgacgcatgc gggcgcatac gatctcgcat tcctgcgctg catcccgaac atgacggtga tggccgcgtc ggacgagaac gagtgtcgcc agatgctgca caccgcgctg cagcagccga acccgaccgc ggtccgttat ccgcgcggcg caggcacggg cgttgcgacc gtcaaggcgt tcaccgagat tccgctcggc aagggcgaag tgcgccgtcg cacgtcgcag ccggacggca agcgcgtcgc gattctcgcg ttcggcacga tggtcgcgcc gtcgcttgcg gccgccgacg cgctcgacgc gaccgtggcg aacatgcgtt tcgtgaagcc gatcgacgcc gagctcgttc gagagctcgc gcagacgcac gactacctcg tcaccgtcga ggaaggctgc gtgatgggcg gcgcgggctc cgcgtgcgtc gaggcgatga tggaaggcgg cgcggtccgt ccggtgctgc agctcggcct gccggaccgc ttcgtcgatc acggcgatcc ggcgaagctg cttgcgatgt gcggtctcga cggcgatggc atcgcgaaat cgattcgcga gcgcttcctg aaccacgcgg cgaacgtcgc gagtccggcg aagcgcgtcg cataa

Details for ButhA.17230.b.A1.GE32773

HARVESTED ON:	6/20/2011
SEQUENCED ON:	5/1/2015
EXPECTED MW:	70kDa
OBSERVED MW:	70kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	76
PERCENT COVERAGE:	58

Validated AA Sequence

MGTLEAQTQG PGSMYDLLKT IDDPADLRRL DRRQLQPLAD ELRAFVLDSV SKTGGHLSSN LGTVELTIAL HYVFNTPDDR IVWDVGHQTY PHKILTGRRD GMKTLRQFDG ISGFPRRSES EYDTFGTAHS STSISAALGM AIGSKLNGDD RFSIAVIGDG AMTAGMAFEA MNNAGVSEDA KLLVILNDND MSISPPVGAL NRHLARLMSG RFYAAARAGV ERVLSVAPPV LELARKLEEH AKGMVVPATL FEEFGFNYIG PIDGHDLDSL IPTLQNIXGT ARXAVPXRXD XXXXGXXLAX XXPXXXXXXX XXXXXXXXXX XXXXXXXXXR RYSAXXCATR RSXMRAWSAS RPRCAKXRAW SSSRSASRSA TTTSALPSST R

Validated NT Sequence

ttattatgcg acgcgcttcg ccggactcgc gacgttcgcc gcgtggttca ggaagcgctc gcgaatcgat ttcgcgatgc catcgccgtc gagaccgcac atcgcaagca gcttcgccgg atcgccgtga tcgacgaagc ggtccggcag gccgagctgc agcaccggac ggaccgcgcc gccttccatc atcgcctcga cgcacgcgga gcccgcgccg cccatcacgc agccttcctc gacggtgacg aggtagtcgt gcgtctgcgc gagctctcga acgagctcgg cgtcgatcgg cttcacgaaa cgcatgttcg ccacggtcgc gtcgagcgcg tcggcggccg caagcgacgg cgcgaccatc gtgccgaacg cgagaatcgc gacgcgcttg ccgtccggct gcgacgtgcg acggcgcact tcgcccttgc cgagcggaat ctcggtgaac gccttgacgg tcgcaacgcc cgtgcctgcg ccgcgcggat aacggaccgc ggtcgggttc ggctgctgca gcgcggtgtg cagcatctgg cgacactcgt tctcgtccga cgcggccatc accgtcatgt tcgggatgca gcgcaggaat gcgagatcgt atgcgcccgc atgcgtcgcg ccgtccgcgc cgacgaggcc cgcgcgatcg atcgcgaaca cgacgggcag gttctgcagc gcgacgtcgt gaatcagctg atcataggcg cgctgcagga aagtcgagta gatcgcgacg acgggcttga gcccttccgt cgcgaggccg cccgcgaacg tcaccgcgtg ctgctcggca atgccgacgt cgtagtagcg ctccgggaag cgcttctcga actcgaccat gcccgancct tcgcgcatcg cgggcgtgat gccgaccacg cgcgcatcnn gctncgncgc gtcgcacann nnttcgccga atacctgcgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gnnnnnnnan nnannnncgg atngncntca gcgagctnnt agccnngtnc nnnntngtca ngacgnnnag gaactgcnnn ncgcgcagtt ccnntgatgt tctgcagcgt cgggatcagc gaatcgagat cgtggccgtc gatcggcccg atgtagttga agccgaattc ctcgaagagc gtcgcgggca ccaccatgcc cttcgcatgc tcctcgagct tgcgcgcgag ctcgagcacg ggcggcgcga cgctcagcac gcgttcgacg cccgcgcgcg cggccgcgta gaagcggccc gacatcaggc gcgcgagatg gcggttcagc gcgccgacgg gcggcgagat cgacatgtcg ttgtcgttca ggatcacgag gagcttcgcg tcctcggaca cgcccgcgtt gttcatcgcc tcgaacgcca tgccggccgt catcgcgccg tcgccgatca ccgcgatcga gaagcggtcg tcgccgttca gcttgctgcc gatcgccatc ccgagcgcag ccgagatcga cgtgctcgag tgcgcggtgc cgaacgtgtc gtattcggat tccgagcggc gcggaaagcc cgagatgccg tcgaactggc gcagcgtctt catcccgtcg cggcggcccg tcaggatctt gtgcggatag gtctggtgac cgacgtccca cacgatccgg tcgtccggcg tgttgaacac gtaatgcagc gcgatcgtca gctcgaccgt gccgagattg gacgacaaat ggccgcccgt cttcgacacg ctgtcgagaa cgaacgcgcg cagctcgtcg gcgagcggct gcagttggcg acgatcgaga cggcgcaggt ccgccgggtc gtcgatggtt ttcagcaagt cgtacatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatgagcc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMYDLLKTID DPADLRRLDR RQLQPLADEL RAFVLDSVSK TGGHLSSNLG TVELTIALHY VFNTPDDRIV WDVGHQTYPH KILTGRRDGM KTLRQFDGIS GFPRRSESEY DTFGTAHSST SISAALGMAI GSKLNGDDRF SIAVIGDGAM TAGMAFEAMN NAGVSEDAKL LVILNDNDMS ISPPVGALNR HLARLMSGRF YAAARAGVER VLSVAPPVLE LARKLEEHAK GMVVPATLFE EFGFNYIGPI DGHDLDSLIP TLQNIKELRG PQFLHVVTKK GQGYKLAEAD PVLYHGPGKF NPAEGIKPSA TPAKKTYTQV FGEWLCDAAE LDARVVGITP AMREGSGMVE FEKRFPERYY DVGIAEQHAV TFAGGLATEG LKPVVAIYST FLQRAYDQLI HDVALQNLPV VFAIDRAGLV GADGATHAGA YDLAFLRCIP NMTVMAASDE NECRQMLHTA LQQPNPTAVR YPRGAGTGVA TVKAFTEIPL GKGEVRRRTS QPDGKRVAIL AFGTMVAPSL AAADALDATV ANMRFVKPID AELVRELAQT HDYLVTVEEG CVMGGAGSAC VEAMMEGGAV RPVLQLGLPD RFVDHGDPAK LLAMCGLDGD GIAKSIRERF LNHAANVASP AKRVA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtacgacttg ctgaaaacca tcgacgaccc ggcggacctg cgccgtctcg atcgtcgcca actgcagccg ctcgccgacg agctgcgcgc gttcgttctc gacagcgtgt cgaagacggg cggccatttg tcgtccaatc tcggcacggt cgagctgacg atcgcgctgc attacgtgtt caacacgccg gacgaccgga tcgtgtggga cgtcggtcac cagacctatc cgcacaagat cctgacgggc cgccgcgacg ggatgaagac gctgcgccag ttcgacggca tctcgggctt tccgcgccgc tcggaatccg aatacgacac gttcggcacc gcgcactcga gcacgtcgat ctcggctgcg ctcgggatgg cgatcggcag caagctgaac ggcgacgacc gcttctcgat cgcggtgatc ggcgacggcg cgatgacggc cggcatggcg ttcgaggcga tgaacaacgc gggcgtgtcc gaggacgcga agctcctcgt gatcctgaac gacaacgaca tgtcgatctc gccgcccgtc ggcgcgctga accgccatct cgcgcgcctg atgtcgggcc gcttctacgc ggccgcgcgc gcgggcgtcg aacgcgtgct gagcgtcgcg ccgcccgtgc tcgagctcgc gcgcaagctc gaggagcatg cgaagggcat ggtggtgccc gcgacgctct tcgaggaatt cggcttcaac tacatcgggc cgatcgacgg ccacgatctc gattcgctga tcccgacgct gcagaacatc aaggaactgc gcggcccgca gttcctgcac gtcgtgacga agaagggaca gggctacaag ctcgctgagg ccgatccggt gctctatcac gggcccggca agttcaaccc ggcggaaggc atcaagccgt ccgcgacgcc ggcgaagaag acctacacgc aggtattcgg cgaatggctg tgcgacgcgg cggagctcga tgcgcgcgtg gtcggcatca cgcccgcgat gcgcgaaggc tcgggcatgg tcgagttcga gaagcgcttc ccggagcgct actacgacgt cggcattgcc gagcagcacg cggtgacgtt cgcgggcggc ctcgcgacgg aagggctcaa gcccgtcgtc gcgatctact cgactttcct gcagcgcgcc tatgatcagc tgattcacga cgtcgcgctg cagaacctgc ccgtcgtgtt cgcgatcgat cgcgcgggcc tcgtcggcgc ggacggcgcg acgcatgcgg gcgcatacga tctcgcattc ctgcgctgca tcccgaacat gacggtgatg gccgcgtcgg acgagaacga gtgtcgccag atgctgcaca ccgcgctgca gcagccgaac ccgaccgcgg tccgttatcc gcgcggcgca ggcacgggcg ttgcgaccgt caaggcgttc accgagattc cgctcggcaa gggcgaagtg cgccgtcgca cgtcgcagcc ggacggcaag cgcgtcgcga ttctcgcgtt cggcacgatg gtcgcgccgt cgcttgcggc cgccgacgcg ctcgacgcga ccgtggcgaa catgcgtttc gtgaagccga tcgacgccga gctcgttcga gagctcgcgc agacgcacga ctacctcgtc accgtcgagg aaggctgcgt gatgggcggc gcgggctccg cgtgcgtcga ggcgatgatg gaaggcggcg cggtccgtcc ggtgctgcag ctcggcctgc cggaccgctt cgtcgatcac ggcgatccgg cgaagctgct tgcgatgtgc ggtctcgacg gcgatggcat cgcgaaatcg attcgcgagc gcttcctgaa ccacgcggcg aacgtcgcga gtccggcgaa gcgcgtcgca taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa